Geographical diversity and genetic relationships among Cedrus species estimated by AFLP (original) (raw)

Abstract

Genetic diversity was described in 17 cedar populations covering the geographical range of the four species of the genus Cedrus. The study was conducted using amplified fragment length polymorphism (AFLP) on haploid tissues (megagametophytes). Eleven selective AFLP primer pairs generated a total of 107 polymorphic amplification products. Correspondence and genetic distance analyses indicated that Cedrus deodara constitutes a separate gene pool from the Mediterranean cedars. Within Mediterranean cedars, we distinguished two groups: the first one is made of Cedrus atlantica, while the second one is made of Cedrus libani and Cedrus brevifolia, these latter two species being genetically similar despite important divergence previously observed for morphological and physiological traits. The lowest intrapopulation variability was found in the two C. deodara populations analyzed. Surprisingly, C. brevifolia, the endemic taxon from the island of Cyprus that is found in small and fragmented populations, showed one of the highest levels of diversity. This unexpected pattern of diversity and differentiation observed for C. brevifolia suggests a recent divergence rather than a relictual, declining population. Patterns of diversity within- and among-populations were used to test divergence and fragmentation hypotheses and to draw conclusions for the conservation of Cedrus gene pools.

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Acknowledgements

We would like to thank Drs. Michel Bariteau (France), Zeki Kaya, Fikret Isik (Turkey), Youssef Bardouka (Syria), Nizar Hani (Lebanon), and Neophyton (Cyprus) for supplying cedar seeds. This work was supported by an INCO-DC program (ERBIC 18CT 970 200).

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Author notes

1. Magida Bou Dagher-Kharrat
   Present address: Département Sciences de la Vie et de la Terre, Université Saint Joseph, Campus Sciences et Technologies, Mar Roukos, Mkalles, BP 1514 Riad el Solh, Beyrouth, 1107 2050, Lebanon
2. Stéphanie Mariette
   Present address: INRA, Unité de recherches Espèces Fruitières et Vigne, Domaine de la Grande Ferrade, 71 avenue Edouard Bourlaux, BP 81, 33883, Villenave d’Ornon Cedex, France

Authors and Affiliations

1. Université Pierre et Marie Curie-Paris6, CNRS FRE 2846, PCMP, Case 156, 4 place Jussieu, Paris, 75005, France
   Magida Bou Dagher-Kharrat & Arnould Savouré
2. UMR Biodiversité Gènes et Ecosystèmes, INRA, 69 route d’Arcachon, 33612, Cestas Cedex, France
   Stéphanie Mariette & Christophe Plomion
3. INRA, UR629 Recherches Forestières Méditerranéennes, Domaine Saint Paul, Site Agroparc, 84914, Avignon Cedex 9, France
   François Lefèvre & Bruno Fady
4. Institut Polytechnique La Salle Beauvais, Rue Pierre Waguet, 60026, Beauvais Cedex, France
   Ghislaine Grenier-de March

Authors

1. Magida Bou Dagher-Kharrat
2. Stéphanie Mariette
3. François Lefèvre
4. Bruno Fady
5. Ghislaine Grenier-de March
6. Christophe Plomion
7. Arnould Savouré

Corresponding author

Correspondence toArnould Savouré.

Additional information

Communicated by D. Grattapaglia

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Dagher-Kharrat, M.B., Mariette, S., Lefèvre, F. et al. Geographical diversity and genetic relationships among Cedrus species estimated by AFLP.Tree Genetics & Genomes 3, 275–285 (2007). https://doi.org/10.1007/s11295-006-0065-x

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• Received: 13 March 2006
• Revised: 04 September 2006
• Accepted: 20 September 2006
• Published: 21 November 2006
• Issue date: July 2007
• DOI: https://doi.org/10.1007/s11295-006-0065-x

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