Interaction Between Calmodulin and Target Proteins (original) (raw)
Abstract
When myosin light chain kinase (MLCK) activity was measured at various different free Ca2+ concentrations, the activity attained abruptively the maximum value within a very narrow concentration range of Ca2+. A result of this phenomenon is shown in Fig.1 (curve e), accompanied with the Ca2+ binding curve of calmodulin (curve a) (1). Since the Ca2+-calmodulin is known to work as an activator of MLCK, a large difference between the enzyme activation curve and the Ca2+ binding curve of calmodulin indicates that Ca2+ effect on the MLCK activity can not directly be explained by the comparison of these two curves. Olwin et al. have already shown that the affinity of Ca2+ to calmodulin was largely enhanced by the addition of MLCK (2). It is expected that the analysis of enhancing effect of MLCK to-the Ca2+ binding to calmodulin may give an answer how the enzyme attains the maximum activity within such a narrow concentration range of Ca2+ and how the Ca2+ concentration is fixed to this range. The Ca2+ binding to calmodulin was measured in the presence of mastoparan (curve c in Fig. 1) (3). Mastoparan is a small peptide composed of 14 amino acid residues, and Malencik and Anderson have reported a strong binding constant of mastoparan to calmodulin (4).
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Authors and Affiliations
- Department of Chemistry and High Resolution, Faculty of Science, Hokkaido University, Sapporo 060, Japan
Koichi Yagi & Michio Yazawa - NMR Laboratory, Faculty of Science, Hokkaido University, Sapporo 060, Japan
Mitsuhiko Ikura & Kunio Hikichi
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- Koichi Yagi
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Editors and Affiliations
- Nagoya University, Nagoya, Japan
H. Hidaka - Swiss Federal Institute of Technology, Zurich, Switzerland
E. Carafoli - Baylor College of Medicine, Houston, Texas, USA
A. R. Means - Mie University School of Medicine, Mie, Japan
T. Tanaka
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© 1989 Plenum Press, New York
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Yagi, K., Yazawa, M., Ikura, M., Hikichi, K. (1989). Interaction Between Calmodulin and Target Proteins. In: Hidaka, H., Carafoli, E., Means, A.R., Tanaka, T. (eds) Calcium Protein Signaling. Advances in Experimental Medicine and Biology, vol 255. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5679-0\_16
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