Prevention of diabetic glomerulopathy in streptozotocin diabetic rats by insulin treatment (original) (raw)

We’re sorry, something doesn't seem to be working properly.

Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Summary

A single antibody radioimmunoassay has been used to measure albumin excretion in 3 groups of female Wistar rats. Two groups had Streptozotocin diabetes and were treated daily with insulin for 6 months. In one of the diabetic groups good glycaemic control was attempted and throughout the 6 months plasma glucose levels were fairly close to normal (92 ± 33 mg/100 ml at 2300 h and 186 ± 9 mg/100 ml at 0800 h). In the other diabetic group poor control was intended and the group had consistent high plasma glucose levels (576 ± 89 mg/100 ml and 460 ± 43 mg/100 ml). The third group was a non-diabetic control group. — Albumin excretion was measured on two occasions: before the induction of diabetes and after 6 months of diabetes. The geometric mean albumin excretion increased from 0.38 to 2.56 mg/24 h in the 18 non-diabetic controls. In the 20 diabetic rats in “good” control the geometric mean albumin excretion increased from 0.37 to 1.58 mg/ 24 h (NS compared with controls) and in the group of 22 rats in poor control albumin excretion increased from 0.35 to 6.54 mg/24 h. — The increase in albumin excretion in rats in poor control differed significantly both from that of the non-diabetic controls (2p = 0.023) and from that of the “well-controlled” diabetic rats (2p = 0.00011).

Article PDF

Similar content being viewed by others

References

  1. Bell E T (1953) Renal vascular disease in diabetes mellitus. Diabetes 2:376–389
    Google Scholar
  2. Hägg E (1974) Renal lesions in rats with long-term alloxan diabetes. A semiquantitative light microscopic study with particular reference to the glomeruli. Acta Pathol Microbiol Scand (A) 82:199–210
    Google Scholar
  3. Hostetter T H, Troy J L, Brenner B M (1978) Glomerular dynamics in rats with diabetes mellitus. Kidney Int 14:725
    Google Scholar
  4. Keen H, Chlouverakis C, Fuller J, Jarrett R J (1969) The concomitants of raised blood sugar: studies in newly-detected hyperglycaemics. II. Urinary albumin excretion, blood pressure and their relation to blood sugar levels. Guys Hosp Rep 118:247–254
    Google Scholar
  5. Knowles H C (1974) Magnitude of the renal failure problem in diabetic patients. Kidney Int 6:S2-S7
    Google Scholar
  6. Mauer S M, Sutherland D E R, Steffes M W, Leonard R J, Najarian J S, Michael A F, Brown D M (1974) Pancreatic islet transplantation. Effects on the glomerular lesions of experimental diabetes in the rat. Diabetes 23:748–753
    Google Scholar
  7. Mauer S M, Brown D M, Matas A J, Steffes M W (1978) Effects of pancreatic islet transplantation on the increased urinary albumin excretion rates in intact and uninephrectomized rats with diabetes mellitus. Diabetes 27:959–964
    Google Scholar
  8. Miles M D, Mogensen C E, Gundersen H J G (1970) Radioimmunoassay for urinary albumin using a single antibody. Scand J Clin Lab Invest 26:5–11
    Google Scholar
  9. Mogensen C E (1976) Renal function changes in diabetes. Diabetes 25:872–879
    Google Scholar
  10. Mogensen C E, Andersen M J F (1975) Increased kidney size and glomerular filtration rate in untreated juvenile diabetes: Normalization by insulin-treatment. Diabetologia 11:221–224
    Google Scholar
  11. Rasch R (1979) Control of blood glucose levels in the streptozotocin diabetic rat using a long-acting heat-treated insulin. Diabetologia 16:185–190
    Google Scholar
  12. Rasch R (1979) Prevention of diabetic glomerulopathy in streptozotocin diabetic rats by insulin treatment. Kidney size and glomerular volume. Diabetologia 16:125–128
    Google Scholar
  13. Rasch R (1979) Prevention of diabetic glomerulopathy in streptozotocin diabetic rats by insulin treatment. Glomerular basement membrane thickness. Diabetologia 16:319–324
    Google Scholar
  14. Rasch R, Mogensen CE (in press) Urinary excretion of albumin and total protein in normal and streptozotocin diabetic rats. Acta Endocrinol (kbh)
  15. Rasch R (1979) Prevention of diabetic glomerulopathy in streptozotocin diabetic rats by insulin treatment. The mesangial regions. Diabetologia 17:243–248
    Google Scholar
  16. Schlichtkrull J (1958) Insulin crystals. Thesis. Ejnar Munksgaards Publishers, Copenhagen, p 97
    Google Scholar
  17. Seyer-Hansen K (1977) Renal hypertrophy in experimental diabetes: Relation to severity of diabetes. Diabetologia 13:141–143
    Google Scholar
  18. Seyer-Hansen K (1978) Renal hypertrophy in experimental diabetes: A comparison to compensatory hypertrophy. Diabetologia 14:325–328
    Google Scholar
  19. Viberti G C, Pickup J C, Jarrett R J, Keen H (1979) Effect of control of blood glucose on urinary excretion of albumin and β 2 microglobulin in insulin-dependent diabetics. N Engl J Med 300:638–641
    Google Scholar
  20. Ørskov H, Olsen T S, Nielsen K, Rafaelsen O J, Lundbæk K (1965) Kidney lesions in rats with severe long-term alloxan diabetes. I. Influence of age, alloxan damage and insulin administration. Diabetologia 1:172–179
    Google Scholar
  21. Østerby R (1972) Morphometric studies of the peripheral glomerular basement membrane in early juvenile diabetes. I. Development of initial basement membrane thickening. Diabetologia 8:84–92
    Google Scholar

Download references

Author information

Authors and Affiliations

  1. Second University Clinic of Internal Medicine, The University Institute of Pathology, Aarhus Kommunehospital, Denmark
    R. Rasch
  2. Department of Cell Biology, Institute of Anatomy, University of Aarhus, Aarhus, Denmark
    R. Rasch

Authors

  1. R. Rasch
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

Rasch, R. Prevention of diabetic glomerulopathy in streptozotocin diabetic rats by insulin treatment.Diabetologia 18, 413–416 (1980). https://doi.org/10.1007/BF00276823

Download citation

Key words