Light and electron microscopic studies on the effect of praziquantel onSchistosoma mansoni, Dicrocoelium dendriticum, andFasciola hepatica (trematoda) in vitro (original) (raw)

Abstract

The fine structure of the tegument of three trematode species,Schistosoma mansoni, Dicrocoelium dendriticum, and_Fasciola hepatica_, was studied by means of light scanning (SEM) and transmission electron microscopy (TEM) after in vitro exposure to 0, 1,10, and 100 μg/ml of the anthelmintic praziquantel for 5, 15, 30, and 60 min. In_S. mansoni_ and_D. dendriticum_ the resulting vacuolization of the tegument was confined to numerous small areas scattered all over the surface of the parasites and this finally led to the disruption of the apical tegumental layer. No changes were found in the tegument of_F. hepatica_ after treatment with praziquantel.

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Abbreviations

B:

Bubbles

BL:

Basement layer

CM:

Circular musculature

GA:

Genital atrium

GC:

Gynaecophoric canal

IB:

Inclusion bodies

IM:

Interstitial material

LM:

Longitudinal musculature

MI:

Mitochondria

OS:

Oral sucker

R:

Ridges

S:

Spine

SP:

Surface pits

T:

Tubercle

TG:

Tegument

V:

Vacuoles

VS:

Ventral sucker

SEM:

Scanning electron microscopy

TEM:

Transmission electron microscopy

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Authors and Affiliations

1. Institut für Zoologie II der Universität Düsseldorf, Universitätsstr. 1, D-4000, Düsseldorf 1, Germany
   B. Becker & H. Mehlhorn
2. Bayer Pharma-Forschungszentrum, Institut für Chemotherapie, D-5600, Wuppertal 1, Germany
   P. Andrews & H. Thomas
3. Institut für Parasitologie der Universität Zürich, CH-8057, Zürich, Switzerland
   J. Eckert

Authors

1. B. Becker
2. H. Mehlhorn
3. P. Andrews
4. H. Thomas
5. J. Eckert

Additional information

Dedicated to Professor G. Piekarski on the occasion of his 70th birthday

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Becker, B., Mehlhorn, H., Andrews, P. et al. Light and electron microscopic studies on the effect of praziquantel on_Schistosoma mansoni, Dicrocoelium dendriticum_, and_Fasciola hepatica_ (trematoda) in vitro.Z. Parasitenkd. 63, 113–128 (1980). https://doi.org/10.1007/BF00927527

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• Received: 21 February 1980
• Published: 01 June 1980
• Issue date: June 1980
• DOI: https://doi.org/10.1007/BF00927527

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