Requirement of malarial protease in the invasion of human red cells by merozoites ofPlasmodium falciparum (original) (raw)
Summary
Highly synchronous cultures of the erythrocyte stages of_Plasmodium falciparum_ were used to determine the effects of a number of protease inhibitors on parasite development and merozoite invasion. Leupeptin, N-tosyl-L-lysyl chloromethylketone and pepstatin at a concentration greater than 0.05 mM were deleterious to both parasite development and merozoite invasion whereas aprotinin, antipain, α-1-antitrypsin and soybean trypsin inhibitor had no effect at a concentration of 0.5 mM. On the other hand, N-tosyl-L-phenylalanyl chloromethylketone and phenylmethylsulfonylfluoride at a concentration of 1 mM and chymostatin at a concentration of 0.15 mM inhibited merozoite invasion but were not deleterious to parasite development. Pretreatment of red cells with these three inhibitors did not block merozoite invasion. These results suggested that a chymotrypsin-like activity of the merozoite is important in the invasion process.
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Authors and Affiliations
- Department of Biochemistry, Faculty of Science, Mahidol University, Rama VI Road, 10400, Bangkok, Thailand
Porn-ngarm Dejkriengkraikhul & Prapon Wilairat
Authors
- Porn-ngarm Dejkriengkraikhul
- Prapon Wilairat
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Dejkriengkraikhul, Pn., Wilairat, P. Requirement of malarial protease in the invasion of human red cells by merozoites of_Plasmodium falciparum_.Z. Parasitenkd. 69, 313–317 (1983). https://doi.org/10.1007/BF00927873
- Accepted: 18 January 1983
- Issue date: May 1983
- DOI: https://doi.org/10.1007/BF00927873