Histochemical evidence of the catecholamine-associated nervous system in certain schistosome cercariae (original) (raw)

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Abstract

The localization of catecholamines was documented in the cercaria of_Schistosoma mansoni_ and_S. japonicum_ by a fluorescent histochmical method using glyoxylic acid (GA). Cell bodies and nerve fibres were spatially visualized in wholemount preparations, and the fluorescent traces were investigated. The nervous system was bilaterally symmetrical, showing a similar formation in both species. A pair of cerebral ganglia with a transverse commussure showed a complex network of fluorophores, and each radiated two sets of anterior and posterior longitudinal nerve cords. A third pair of longitudinal nerve cords was observed in the most dorsal area. A posterior transverse commissure was seen to connect the posterior longitudinal cords, and the posterior terminals of the postero-ventral cords communicated with the tail cords. The glyoxylic acid-induced fluorescence (GAIF) method was demonstrated to be quite suitable for neuroanatomical and neurophysiological investigations of larval forms.

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References

• Bennett J, Bueding E (1971) Localization of biogeneic amines in_Schistosoma mansoni_. Comp Biochem Physiol A39:859–867
  Google Scholar
• Björklund A, Lindvall O, Svensson LÅ (1972) Mechanisms of fluorophore formation in the histochemical glyoxylic acid method for monoamines. Histochemie 32:113–131
  Google Scholar
• Bruckner DA, Voge M (1974) The nervous system of larval_Schistosoma mansoni_ as revealed by acetylcholinesterase staining. J Parasitol 60:437–446
  Google Scholar
• Chiba T, Hwang BH, Williams TH (1976) A method for studying glyoxylic acid induced fluorescence and ultrastructure of monoamine neurons. Histochmistry 49:95–106
  Google Scholar
• Chou TCT, Bennett J, Bueding E (1972) Occurrence and concentrations of biogenic amines in trematodes. J Parasitol 58:1098–1102
  Google Scholar
• Fripp PJ (1967) Histochemical localization of esterase activity in schistosomes. Exp Parasitol 21:380–390
  Google Scholar
• Gianutsos G, Bennett JL (1977) The regional distribution of dopamine and norepinephrine in_Schistosoma mansoni_ and_Fasciola hepatica_. Comp Biochem Physiol C58:157–159
  Google Scholar
• König R (1979) Consecutive demonstration of catecholamines and dopamine-β-hydroxylase within the same specimen. Histochemistry 61:301–305
  Google Scholar
• Korkala O, Waris T (1977) The acetylcholinesterase reaction and catecholamine fluorescence in the glomus cells of rat carotid body. Experientia 33:1363–1364
  Google Scholar
• Lewert RM, Hopkins DR (1965) Cholinesterase activity in_Schistosoma mansoni_ cercariae. J Parasitol 51:616
  Google Scholar
• Lindvall O, Björklund A (1974) The glyoxylic acid fluorescence histochemical method: a detailed account of the methodology for the visualization of central catecholamine neurons. Histochemistry 39:97–127
  Google Scholar
• Lindvall O, Björklund A, Svensson LÅ (1974) Fluorophore formation from catecholamines and related compounds in the glyoxylic acid fluorescence histochemical method. Histochemistry 39:197–227
  Google Scholar
• Machado CRS, Machado ABM, Pellegrino J (1972) Catecholamine-containing neurons in_Schistosoma mansoni_. Z Zellforsch 124:230–237
  Google Scholar
• Mansour TE (1984) Serotonin receptors in parasitic worms. Adv Parasitol 23:1–36
  Google Scholar
• Nakajima Y (1987) Localization of catecholaminergic nerves in larval echinoderms. Zool Sci 4:293–299
  Google Scholar
• Niewiadomska K, Moczoń T (1982) The nervous system of_Diplostomun pseudospathaceum_ Niewiadomska, (Digenea, Diplostomatidae): I. Nervous system and chaetotaxy in the cercaria. Z Parasitenkd 68:295–304
  Google Scholar
• Pax RA, Siefker C, Bennett JL (1984)Schistosoma mansoni: differences in acetylcholine, dopamine, and serotonin control of circular and longitudinal parasite muscles. Exp Parasitol 58:314–324
  Google Scholar
• Reger JF (1976) Studies on the fine structure of cercarial tail muscle of_Schistosoma_ sp. (Trematoda). J Ultrastruct Res 57:77–86
  Google Scholar
• Sharpe MJ, Atkinson HJ (1980) Improved visualization of dopaminergic neurons in nematodes using the glyoxylic acid fluorescence method. J Zool 190:273–284
  Google Scholar
• Smyth JD, Halton DW (1983) The physiology of trematodes, 2nd edn. Cambridge University Press, Cambridge London New York New Rochelle Melbourne Sydney
  Google Scholar
• Tiekotter KL (1988) Histofluorescent and ultrastructural identification of aminergic processes in the opisthaptor of the marine monogene,Microcotyle sebastis (Polyopisthocotylea: Microcotylinae). Proc Helminthol Soc Wash 55:229–245
  Google Scholar
• Tomosky TK, Bennett JL, Bueding E (1974) Tryptaminergic and dopaminergic responses of_Schistosoma mansoni_. J Pharmacol Exp Ther 190:260–271
  Google Scholar

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Authors and Affiliations

1. Department of Parasitology, School of Medicine, University of the Ryukyus, 903-01, Nishihara, Okinawa, Japan
   Y. Orido

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Orido, Y. Histochemical evidence of the catecholamine-associated nervous system in certain schistosome cercariae.Parasitol Res 76, 146–149 (1989). https://doi.org/10.1007/BF00930837

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• Accepted: 15 August 1989
• Issue date: February 1989
• DOI: https://doi.org/10.1007/BF00930837

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