Gametocytogenesis induction in culturedPlasmodium falciparum and further development of the gametocytes to ookinetes in prolonged culture (original) (raw)

Abstract

Gametocytogenesis induction medium (RPMI-FSC reactive medium), prepared with a mixture of the culture supernatant from anti-Plasmodium falciparum antibody-producing hybridoma cells and hybridoma cell lysate, consistently induced gametocytogenesis in cultured_P. falciparum_. Gametocytogenesis was induced in three strains (0662, FCB 1, and FCR-3) and one clone (R·FCR-3) as has previously been described in two other strains (Ono et al. 1986). Both caffeine (2 m_M_/ml) and calmodulin (5 μg/ml) enhanced the effects of RPMI-FSC reactive medium. Furthermore, gametocytes that appeared after treatment with RPMI-FSC reactive medium continued their development to ookinete formation in candle jar culture with Waymouth's medium.

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Authors and Affiliations

1. Department of Protozoology, Research Institute for Microbial Diseases, Osaka University, 565, Suita, Osaka, Japan
   Tadasuke Ono & Toshio Nakabayashi

Authors

1. Tadasuke Ono
2. Toshio Nakabayashi

Additional information

This study was supported in part by two Grants-in-Aid for Scientific Research (no. 61570193, 1986–1988; no. 61480140, 1986–1988) from the Ministry of Education, Science and Culture of Japan

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Ono, T., Nakabayashi, T. Gametocytogenesis induction in cultured_Plasmodium falciparum_ and further development of the gametocytes to ookinetes in prolonged culture.Parasitol Res 75, 348–352 (1989). https://doi.org/10.1007/BF00931129

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• Accepted: 01 September 1988
• Issue date: May 1989
• DOI: https://doi.org/10.1007/BF00931129

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