A comparative ultrastructural and histochemical study of the metacercarial cyst walls of four species ofParagonimus (Troglotrematidae: Trematoda) (original) (raw)

Abstract

The metacercarial cyst walls of_Paragonimus westermani, P. miyazakii, P. ohirai_, and_P. iloktsuenensis_ were examined using ultrastructural and histochemical techniques. The cyst walls of_P. westermani, P. miyazakii_, and_P. ohirai_ were found to have five distinct layers, whereas the wall of_P. iloktsuenensis_ had only two. The so-called outer cyst wall recognized on light microscopy by Miyazaki (1961) was composed of layers I–III and the so-called inner cyst wall comprised layers IV and V. The outermost layer (I) consisted of collagen fibers and probably originated from the host. Layer III detached easily from layer IV on removal of the cysts from the host tissues. Layer IV was composed of proteoglycans, and layer V consisted of protein alone. A regular hexagonal pattern in layer V appeared to be characteristic of_P. westermani_. Although the basic morphological patterns observed in the layers constituting the cyst walls were similar in all species examined, the thickness of each layer was apparently different. This characteristic seems to be a suitable criterion for the identification of metacercarial cysts of_Paragonimus_ species.

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Authors and Affiliations

1. Department of Parasitology, Faculty of Medicine, Kyushu University, 812, Fukuoka, Japan
   Y. S. Lou, T. Fujino, K. Morita & Y. Ishii

Authors

1. Y. S. Lou
2. T. Fujino
3. K. Morita
4. Y. Ishii

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Lou, Y.S., Fujino, T., Morita, K. et al. A comparative ultrastructural and histochemical study of the metacercarial cyst walls of four species of_Paragonimus_ (Troglotrematidae: Trematoda).Parasitol Res 78, 457–462 (1992). https://doi.org/10.1007/BF00931563

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• Accepted: 10 March 1992
• Issue date: June 1992
• DOI: https://doi.org/10.1007/BF00931563

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