Schistosoma mansoni: The effects of a subcurative dose of praziquantel on the ultrastructure of worms in vivo (original) (raw)
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Summary
The in vivo effects of a subcutaneously-administered subcurative dose (200mg/kg body weight of mouse) of praziquantel on the structure of_Schistosoma mansoni_ was investigated. Worms were collected at varying times post-treatment and processed for both SEM and TEM examination.
Praziquantel caused extensive structural changes to both male and female worms within 15 min of treatment although variations in the amount of drug-induced damaged was observed between male and female worms.
In female worms although some tegumental vacuolation was observed within 15 min, the major structural change was an often extensive vacuolisation of the subtegumental tissues followed by varying degrees of structural disruption to the subtegumental and gastrodermal musculature.
In male worms the initial effects were a vacuolisation of parts of the dorsal tegument and loss of tegumental cytoplasm due to the pinching off of evaginations of the outer surface.
With increasing time post-treatment there was an increase in the amount of tegumental damage, particularly in male worms, with total disruption of parts of the outer surface occurring. Also in male worms there was an increase in the amounts of vacuolisation of the parenchymal tissues and in the degree of structural damage to the musculature. In those female worms where subtegumental damage was not extensive changes in the structure of the differentiating vitelline cells were noted.
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References
- Andrews P (1978) Effect of praziquantel on the metabolism of_Schistosoma mansoni_. 4th International Congress of Parasitology. Warsaw. Section D: 5–6
- Andrews P (1981) A summary of the efficacy of praziquantel against schistosomes in animal experiments and notes on its mode of action. Arzneim-Forsch/Drug Res 31:538–541
Google Scholar - Andrews P, Thomas H (1979) The effect of praziquantel on_Hymenolepis diminuta_ in vitro. Tropenmed Parasit 30:391–400
Google Scholar - Andrews P, Thomas H, Weber H (1980) The in vitro uptake of14C-praziquantel by cestodes, trematodes and a nematode. J Parasitol 66:920–925
Google Scholar - Becker B, Mehlhorn H, Andrews P, Eckert J (1980a) Light and electron microscopic studies on the effect of praziquantel on_Schistosoma mansoni, Dicrocoelium dendriticum_, and_Fasciola hepatica_ (Trematoda) in vitro. Z Parasitenkd 63:113–128
Google Scholar - Becker B, Mehlhorn H, Andrews P, Thomas H (1980b) Scanning and transmission electron microscope studies on the efficacy of praziquantel on_Hymenolepis nana_ (Cestoda) in vitro. Z Parasitenkd 61:121–133
Google Scholar - Becker B, Mehlhorn H, Andrews P, Thomas H (1981) Ultrastructural investigations on the effect of praziquantel on the tegument of five species of cestodes. Z Parasitenkd 64:257–269
Google Scholar - Coles GC (1979) The effect of praziquantel on_Schistosoma mansoni_. J Helminthol 53:31–33
Google Scholar - Duvall RH, Dewitt WB (1967) An improved perfusion technique for recovering adult schistosomes from laboratory animals. Am J Trop Med Hyg 16:483–486
Google Scholar - Erasmus DA (1973) A comparative study of the reproductive system of mature, immature and “unisexual” female_Schistosoma mansoni_. Parasitology 67:165–183
Google Scholar - Erasmus DA, Pipiel I (1980)Schistosoma mansoni: Drug induced changes in the cell population of the vitelline gland. Exp Parasitol 50:171–187
Google Scholar - Fetterer RH, Pax RA, Bennett JL (1980) Praziquantel, potassium and 2,4-dinitrophenol: Analysis of their action on the musculature of_Schistosoma mansoni_. Eur J Pharmacol 64:31–38
Google Scholar - Gönnert R (1955) Schistosomiasis — Studien. III. Über die Einwirkung von Miracil D auf_Schistosoma mansoni_ im Mäuseversuch und die Verteilung des Pigmentes in der Wirtsleber. Z Tropenmed 6:257–279
Google Scholar - Gönnert R, Andrews P (1977) Praziquantel a new broad — spectrum antischistosomal agent. Z Parasitenkd 52:129–150
Google Scholar - Hockley DJ (1973) Ultrastructure of the tegument of Schistosoma. Adv Parasitol 11:233–305
Google Scholar - Kusel JR, Sher A, Perez H, Clegg JA, Smithers SR (1975) Use of radioisotopes in the study of specific schistosome membrane antigens. In: “Isotopes and radiation in parasitology”. IV. Vienna: Int Atomic Energy Agency
Google Scholar - Magzoub M (1971) Electron microscopy of normal and treated_Schistosoma mansoni_. Sudan Med J 9:178–182
Google Scholar - McLaren DJ (1980)Schistosoma mansoni: The parasite surface in relation to host immunity. In: “Tropical Medicine Research Studies 1”. Research Studies press: John Wiley Press, Chichester
Google Scholar - Mehlhorn H, Becker B, Andrews P, Thomas H, Frenkel JK (1981) In vivo and in vitro experiments on the effects of praziquantel on_Schistosoma mansoni_. Arzneim-Forsch/Drug Res 31:544–554
Google Scholar - Moore GA (1977) Ultrastructural changes due to treatment in vivo of_Schistosoma mansoni_ with Hycanthone. Trans R Soc Trop Med Hyg 71:115
Google Scholar - Morris GP (1970) The effects of 1,7- bis (p-aminophenoxy) heptane on the ultrastructure of adult_Schistosoma mansoni_. J Parasitol 56: section II 241–242
Google Scholar - Otubanjo OA (1981)Schistosoma mansoni: Astiban-induced damage to tegument and the male reproductive system. Exp Parasitol 52:161–170
Google Scholar - Oyerinde JPO (1976) The effects of 1,7 bis (p-aminophenoxy) heptane on adult_Schistosoma mansoni_ in mice. Nigerian Med J 6:392–397
Google Scholar - Pax RA, Bennett JL, Fetterer RH (1978) A benzodiazepine derivative and praziquantel: effects on musculature of_Schistosoma mansoni_ and_Schistosoma japonicum_. Naunyn-Schmiedeberg's Arch Pharmacol 304:309–315
Google Scholar - Perez H, Terry RJ (1973) The killing of adult_Schistosoma mansoni_ in vitro in the presence of antisera to host antigenic determinants and peritoneal cells. Int J Parasitol 3:499–503
Google Scholar - Simpson AJG, McLaren DJ (1982)Schistosoma mansoni: tegumental damage as a consequence of lectin binding. Exp Parasitol 53:105–116
Google Scholar - Smithers SR, Terry RJ (1965) Infection of laboratory hosts with cercariae of_Schistosoma mansoni_ and the recovery of adult worms. Parasitology 55:695–700
Google Scholar - Smithers SR, Terry RJ (1967) Resistance to experimental infection with_Schistosoma mansoni_ in rhesus monkeys induced by the transfer of adult worms. Trans R Soc Trop Med Hyg 61:517–533
Google Scholar - Thomas H, Gönnert R (1977) The efficacy of praziquantel against cestodes in animals. Z Parasitenkd 52:117–127
Google Scholar - Threadgold LT, Brennan G (1978)Fasciola hepatica: Basal infolds and associated vacuoles of the tegument. Exp Parasitol 46:300–316
Google Scholar - Voge M, Bueding E (1980)Schistosoma mansoni: tegumental surface alterations induced by subcurative doses of the schistosomicide amoscanate. Exp Parasitol 50:251–259
Google Scholar - Watts SDM, Orpin A, MacCormick C (1979) Lysosomes and tegument pathology in the chemotherapy of schistosomiasis with 1,7- bis (p-aminophenoxy) heptane (153 C51). Parasitology 78:287–294
Google Scholar - Webbe G, James C (1977) A comparison of the susceptibility to praziquantel of_Schistosoma haematobium, S. japonicum, S. mansoni, S. intercalatum_ and_S. mattheei_ in hamsters. Z Parasitenkd 52:169–177
Google Scholar - Wilson RA, Barnes PE (1974) An in vitro investigation of dynamic processes occurring in the schistosome tegument, using compounds known to disrupt secretory processess. Parasitology 68:259–270
Google Scholar - Wilson RA, Barnes PE (1977) The formation and turnover of the membranocalyx of the tegument of_Schistosoma mansoni_. Parasitology 74:61–71
Google Scholar - Xiao SH, Wang CY, Jiao PY, Yu YG, Yuan XJ (1981a) Effect of praziquantel on glycogen content and (1-14C) glucose uptake in_Schistosoma japonicum_. Acta Pharmacologia Sinica 2:204–211
Google Scholar - Xiao SH, Yu YG, Wang CY, Jiao PY, Yuan XJ, Liang Y (1981b) The uptake and distribution of3H-pyquiton in_Schistosoma japonicum_. Acta Pharmaceutica Sinica 16:488–493
Google Scholar
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Authors and Affiliations
- Department of Zoology, University College, CF1 1XL, Cardiff, Wales, UK
M. K. Shaw & D. A. Erasmus
Authors
- M. K. Shaw
- D. A. Erasmus
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Shaw, M.K., Erasmus, D.A. Schistosoma mansoni: The effects of a subcurative dose of praziquantel on the ultrastructure of worms in vivo.Z. Parasitenkd. 69, 73–90 (1983). https://doi.org/10.1007/BF00934012
- Accepted: 20 September 1982
- Issue date: January 1983
- DOI: https://doi.org/10.1007/BF00934012