PIG-B: A homemade monophasic cocktail for the extraction of RNA (original) (raw)

Abstract

An inexpensive monophasic reagent has been developed for the extraction of total RNA from cells or tissues. The main ingredients of the reagent arePhenol,Isoamyl alcohol,Guanidinium isothiocyanate, andBeta-mercaptoethanol (PIG-B). The quality and yield of RNA obtained by this reagent is at par with that obtained by TRIzol, an expensive but widely used monophasic reagent available commercially. The complete composition and method of preparation of PIG-B is provided to aid preparation of the reagent in the laboratory.

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References

  1. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1982)Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Gold Spring Harbor, NY.
    Google Scholar
  2. Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.Anal. Biochem. 162, 156–159.
    Article PubMed CAS Google Scholar
  3. Chomczynski, P. (1993) A reagent for the singlestep simultaneous isolation of RNA, DNA, and proteins from cell and tissue samples.Biotechniques 15, 532–536.
    PubMed CAS Google Scholar
  4. Sarkar, G. and Bolander, M. E. (1994) The “Looped Oligo” method for generating reference molecules for quantitative PCR.Biotechniques 17, 864–866.
    PubMed CAS Google Scholar
  5. Sarkar, G. and Sommer, S. S. (1989) Access to a messenger RNA sequence or its protein product is not limited by tissue or species specificity.Science 244, 331–334.
    Article PubMed CAS Google Scholar
  6. Kirby, K. S. (1956) A new method for the isolation of nucleic acids from mammalian tissues.Biochem. J. 64, 405–411.
    PubMed CAS Google Scholar

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Authors and Affiliations

  1. Department of Orthopedics, Mayo Clinic, 3-15, Medical Sciences Building, Rochester, MN
    Kristy Weber, Mark E. Bolander & Gobinda Sarkar

Authors

  1. Kristy Weber
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  2. Mark E. Bolander
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  3. Gobinda Sarkar
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Correspondence toGobinda Sarkar.

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Weber, K., Bolander, M.E. & Sarkar, G. PIG-B: A homemade monophasic cocktail for the extraction of RNA.Mol Biotechnol 9, 73–77 (1998). https://doi.org/10.1007/BF02752699

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