Newly found selenium-containing proteins in the tissues of the rat (original) (raw)

Abstract

The Se-containing proteins in 27 tissues of the rat were investigated by in vivo labeling with75Se-selenite, separation of the tissue homogenate proteins by SDS-polyacrylamide gel electrophoresis, and determination of the labeled proteins by autoradiography. By using Se-depleted rats and a75Se-tracer with a high specific activity, Se compounds present at only very low concentrations could be detected. Besides the 13 Se-containing proteins previously described, for which apparent molecular masses of 12, 15, 18, 20, 22, 25, 28, 34, 56, 60, 65, 70, and 75 kD have been found here, a further 1575Se-labeled bands, with apparent molecular masses of 8, 10, 15.5, 16.5, 24, 32, 34.5, 38, 40, 41, 44, 45, 46.5, 53 and 116 kD could be distinguished. Two-dimensional separation of the kidney homogenate proteins showed that some of the Se-containing bands could be resolved into several labeled spots. Most of the newly found compounds were present in various tissues, but with some the enrichment in certain tissues suggested specific sites of action.

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References

  1. J. T. Rotruck, A. L. Pope, H. E. Ganther, A. B. Swanson, D. G. Hafeman, and W. G. Hoekstra,Science 179, 588–590 (1973).
    Article PubMed CAS Google Scholar
  2. K. Takahashi, N. Avissar, J. Whitin, and H. Cohen,Arch. Biochem. Biophys. 256, 677–686 (1987).
    Article PubMed CAS Google Scholar
  3. F. F. Chu, J. H. Doroshow, and R. S. Esworthy,J. Biol. Chem. 268, 571–576 (1993).
    Google Scholar
  4. F. Ursini, M. Maiorino, and C. Gregolin,Biochim. Biophys. Acta 839, 62–70 (1985).
    PubMed CAS Google Scholar
  5. D. Behne, A. Kyriakopoulos, H. Meinhold, and J. Köhrle,Biochem. Biophys, Res. Commun. 173, 1143–1149 (1990).
    Article CAS Google Scholar
  6. J. R. Arthur, F. Nicol, and G. J. Beckett,Biochem. J. 272, 537–540 (1990).
    PubMed CAS Google Scholar
  7. M. J. Berry, L. Banu, and P. R. Larsen,Nature 349, 438–440 (1991).
    Article PubMed CAS Google Scholar
  8. W. Croteau, S. L. Whittemore, M. J. Schneider, and D. L. St. Germain,J. Biol. Chem. 270, 16569–16575 (1995).
    Article PubMed CAS Google Scholar
  9. R. Read, T. Bellew, J. G. Yang, K. E. Hill, I. S. Palmer, and R. F. Burk,J. Biol. Chem. 265, 17899–17905 (1990).
    PubMed CAS Google Scholar
  10. H. I. Calvin, G. W. Cooper, and E. Wallace,Gamete Res. 4, 139–149 (1981).
    Article CAS Google Scholar
  11. S. C. Vendeland, M. A. Beilstein, C. L. Chen, O. N. Jensen, E. Barofsky, and P. D. Whanger,J. Biol. Chem. 268, 17103–17107 (1993).
    PubMed CAS Google Scholar
  12. M. P. Bansal, R. G. Cook, K. G. Danielson, and D. Medina,J. Biol. Chem. 264, 13780–13784 (1989).
    PubMed CAS Google Scholar
  13. M. P. Bansal, T. Mukhopadhyay, J. Scott, R. G. Cook, R. Mukhopadhyay, and D. Medina,Carcinogenesis 11, 2071–2073 (1990).
    Article PubMed CAS Google Scholar
  14. W. C. Hawkes, E. C. Wilhelmsen, and A. L. Tappel,J. Inorg. Biochem. 23, 77–92 (1985).
    Article PubMed CAS Google Scholar
  15. K. G. Danielson and D. Medina,Cancer Res. 46, 4582–4589 (1986).
    PubMed CAS Google Scholar
  16. H. I. Calvin, K. Grosshans, S. R. Musicant-Shikora, and S. I. Turner,J. Reprod. Fert. 81, 1–11 (1987).
    Article CAS Google Scholar
  17. D. Behne, H. Hilmert, S. Scheid, H. Gessner, and W. Elger,Biochim. Biophys. Acta 966, 12–21 (1988).
    PubMed CAS Google Scholar
  18. J. K. Evenson and R. A. Sunde,Proc. Soc. Exp. Biol. Med. 187, 169–180 (1988).
    PubMed CAS Google Scholar
  19. D. Behne, S. Scheid, A. Kyriakopoulos, and H. Hilmert,Biochim. Biophys. Acta 1033, 219–225 (1990).
    PubMed CAS Google Scholar
  20. R. B. Hubbell, L. B. Mendel, and A. J. Wakeman,J. Nutr. 14, 273–285 (1937).
    CAS Google Scholar
  21. U. K. Laemmli,Nature 227, 680–685 (1970).
    Article PubMed CAS Google Scholar
  22. J. Klose, in_Modern Methods in Protein Chemistry_, H. L. Tschesche, ed., de Gruyter, Berlin, pp. 49–78 (1983).
    Google Scholar
  23. D. Behne, A. Kyriakopoulos, S. Scheid, and H. Gessner,J. Nutr. 121, 806–814 (1991).
    PubMed CAS Google Scholar
  24. C. Weiss-Nowak, A. Kyriakopoulos, and D. Behne,Fresenius J. Anal. Chem. 343, 92 (1992).
    Article Google Scholar
  25. I. Chambers, J. Frampton, P. Goldfarb, N. Affara, W. McBain, and P. R. Harrison,EMBO J. 5, 1221–1227 (1986).
    PubMed CAS Google Scholar

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Authors and Affiliations

  1. Department “Trace Elements in Health and Nutrition”, Hahn-Meitner-Institut, Glienicker Str. 100, D-14109, Berlin, Germany
    Dietrich Behne, Antonios Kyriakopoeulos, Christian Weiss-Nowak, Margrit Kalckloesch, Christian Westphal & Hildegard Gessner

Authors

  1. Dietrich Behne
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  2. Antonios Kyriakopoeulos
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  3. Christian Weiss-Nowak
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  4. Margrit Kalckloesch
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  5. Christian Westphal
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  6. Hildegard Gessner
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Correspondence toDietrich Behne.

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Behne, D., Kyriakopoeulos, A., Weiss-Nowak, C. et al. Newly found selenium-containing proteins in the tissues of the rat.Biol Trace Elem Res 55, 99–110 (1996). https://doi.org/10.1007/BF02784172

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