Changes in Sodium or Glucose Filtration Rate Modulate Expression of Glucose Transporters in Renal Proximal Tubular Cells of Rat (original) (raw)

Abstract.

Renal glucose reabsorption is mediated by luminal sodium-glucose cotransporters (SGLTs) and basolateral facilitative glucose transporters (GLUTs). The modulators of these transporters are not known, and their substrates glucose and Na+ are potential candidates. In this study we examined the role of glucose and Na+ filtration rate on gene expression of glucose transporters in renal proximal tubule. SGLT1, SGLT2, GLUT1 and GLUT2 mRNAs were assessed by Northern blotting; and GLUT1 and GLUT2 proteins were assessed by Western blotting. Renal cortex and medulla samples from control rats (C), diabetic rats (D) with glycosuria, and insulin-resistant 15-month old rats (I) without glycosuria; and from normal (NS), low (LS), and high (HS) Na+-diet fed rats were studied. Compared to C and I rats, D rats increased (P < 0.05) gene expression of SGLT2 by ∼36%, SGLT1 by ∼20%, and GLUT2 by ∼100%, and reduced (P < 0.05) gene expression of GLUT1 by more than 50%. Compared to NS rats, HS rats increased (P < 0.05) SGLT2, GLUT2, and GLUT1 expression by ∼100%, with no change in SGLT1 mRNA expression, and LS rats increased (P < 0.05) GLUT1 gene expression by ∼150%, with no changes in other transporters. In summary, the results showed that changes in glucose or Na+ filtrated rate modulate the glucose transporters gene expression in epithelial cells of the renal proximal tubule.

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Authors and Affiliations

1. Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil, , , , , , BR
   S. Vestri, M.M. Okamoto, H.S. de Freitas, R. Aparecida dos Santos, M.T. Nunes & U.F. Machado
2. Department of Biomedical Sciences, School of Veterinary Medicine, Hokkaido University, Sapporo, Japan, , , , , , JP
   M. Morimatsu
3. Department of Nephrology, School of Medicine, São Paulo University, São Paulo, Brazil, , , , , , BR
   J.C. Heimann

Authors

1. S. Vestri
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2. M.M. Okamoto
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3. H.S. de Freitas
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4. R. Aparecida dos Santos
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5. M.T. Nunes
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6. M. Morimatsu
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7. J.C. Heimann
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8. U.F. Machado
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Received: 14 July 2000/Revised: 8 March 2001

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Vestri, S., Okamoto, M., de Freitas, H. et al. Changes in Sodium or Glucose Filtration Rate Modulate Expression of Glucose Transporters in Renal Proximal Tubular Cells of Rat.J. Membrane Biol. 182, 105–112 (2001). https://doi.org/10.1007/s00232-001-0036-y

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• Issue Date: July 2001
• DOI: https://doi.org/10.1007/s00232-001-0036-y