Fluorescence imaging with near-infrared light: new technological advances that enable in vivo molecular imaging (original) (raw)
Abstract.
A recent development in biomedical imaging is the non-invasive mapping of molecular events in intact tissues using fluorescence. Underpinning to this development is the discovery of bio-compatible, specific fluorescent probes and proteins and the development of highly sensitive imaging technologies for in vivo fluorescent detection. Of particular interest are fluorochromes that emit in the near infrared (NIR), a spectral window, whereas hemoglobin and water absorb minimally so as to allow photons to penetrate for several centimetres in tissue. In this review article we concentrate on optical imaging technologies used for non-invasive imaging of the distribution of such probes. We illuminate the advantages and limitations of simple photographic methods and turn our attention to fluorescence-mediated molecular tomography (FMT), a technique that can three-dimensionally image gene expression by resolving fluorescence activation in deep tissues. We describe theoretical specifics, and we provide insight into its in vivo capacity and the sensitivity achieved. Finally, we discuss its clinical feasibility.
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Authors and Affiliations
- Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Building 149, 13th Street 5406, Charlestown MA 02129–2060, USA, , , , ,
Vasilis Ntziachristos, Christoph Bremer & Ralph Weissleder
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- Vasilis Ntziachristos
You can also search for this author inPubMed Google Scholar - Christoph Bremer
You can also search for this author inPubMed Google Scholar - Ralph Weissleder
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Ntziachristos, V., Bremer, C. & Weissleder, R. Fluorescence imaging with near-infrared light: new technological advances that enable in vivo molecular imaging.Eur Radiol 13, 195–208 (2003). https://doi.org/10.1007/s00330-002-1524-x
- Received: 23 March 2002
- Accepted: 15 April 2002
- Issue Date: January 2003
- DOI: https://doi.org/10.1007/s00330-002-1524-x