Carnosic acid reduces cytokine-induced adhesion molecules expression and monocyte adhesion to endothelial cells (original) (raw)

Abstract

Background

Expression of cell adhesion molecules on the endothelium and the attachment of monocytes to endothelium may play a major role in the early atherogenic process.

Aim of the study

We investigated the effects of carnosic acid on the adhesion of U937 cells to IL-1β-treated human umbilical vein endothelial cells (HUVECs), as well as on the expression of adhesion molecules.

Results

Our data showed that pretreatment with 10 and 20 µmol/l carnosic acid significantly reduced the number of U937 cells adhering to IL-1β-treated HUVECs. In addition, we found that 20 µmol/l carnosic was more effective than 10 µmol/l carnosic acid at inhibiting expression of cell adhesion molecules (ICAM-1, VCAM-1, and E-selectin), the nuclear translocation of NF-κB subunits p65 and p50, and the production of ROS in IL-1β-stimulated HUVECs.

Conclusions

We conclude that carnosic acid inhibits IL-1β-induced ICAM-1, VCAM-1 and E-selectin expression in HUVECs through a mechanism that involves NFκB. We propose that the reduction in binding of human monocytic cell line U937 to IL-1β-treated HUVECs is due to the anti-inflammatory properties of carnosic acid.

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Acknowledgment

This work was supported by the National Science Council of Taiwan (NSC 96-2320-B-309-004-MY2) and Changhua Christian Hospital (9-CCH-CJCU-02).

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Authors and Affiliations

  1. Dept. of Health Science, Chang Jung Christian University, 396 Chang Jung Rd., Sec. 1, Kway Jen, Tainan, 71101, Taiwan
    Ya-Mei Yu PhD, MPH
  2. Dept. of Nutrition, China Medical University, Taichung, Taiwan
    Chin-Huei Lin MS
  3. Dept. of Biochemistry, China Medical University, Taichung, Taiwan
    Hsu-Chin Chan PhD
  4. Dept. of Obstetrics and Gynecology, Changhua Christian Hospital, Changhua, Taiwan
    Hong-Der Tsai MD

Authors

  1. Ya-Mei Yu PhD, MPH
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  2. Chin-Huei Lin MS
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  3. Hsu-Chin Chan PhD
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  4. Hong-Der Tsai MD
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Corresponding author

Correspondence toYa-Mei Yu PhD, MPH.

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Yu, YM., Lin, CH., Chan, HC. et al. Carnosic acid reduces cytokine-induced adhesion molecules expression and monocyte adhesion to endothelial cells.Eur J Nutr 48, 101–106 (2009). https://doi.org/10.1007/s00394-008-0768-x

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