The initial immune response during experimental cysticercosis is of the mixed Th1/Th2 type (original) (raw)

Abstract

The immunological events that occur during the initial stages of experimental cysticercosis are not known. The studies presented here examined the cytokines produced by peritoneal exudate cells (PECs), splenocytes and mesenteric lymph node (MLN) cells during the first week of infection with larval Taenia crassiceps in BALB/cJ mice. Proliferation assays determined that the earliest time when antigen-specific responses could be measured was 5 days post-infection. Concanavalin A (ConA) stimulation of host cells elicited an initial burst of IL-4 production at 24 h of infection and ConA-stimulated Th2-type cytokine production is predominant by 7 days post-infection. Thus, there are responses at day 1 of infection that seem to promote a Th2-type response. Stimulation of MLN cells, splenocytes and PECs with larval antigens supported previous reports of mixed Th1/Th2-type cytokine production with increases in interleukin (IL)-4, IL-10 and interferon (IFN)-γ. Ex vivo IFN-γ production by PECs from infected mice was increased at 3, 5 and 7 days post-infection, whereas at these times reduced ex vivo IL-10 production was observed. This ex vivo IFN-γ response preceded an increasing IL-10 production by PECs between 3 and 7 days post-infection in parasite-specific and ConA-induced proliferation assays. Thus, infection with larval T. crassiceps results in an initial response mediated by IFN-γ that is quickly followed by an increase in IL-10 production and subsequent reduction in the amount of IFN-γ being produced.

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References

• Bojalil R, Terrazas L, Govezensky T, Sciutto E, Larralde, C (1993) Thymus-related cellular immune mechanisms in sex associated resistance to experimental murine cysticercosis (Taenia crassiceps). J Parasitol 79:384–389
  PubMed Google Scholar
• Cruz-Revilla C, Rosas G, Fragoso G, Lopez-Casillas F, Toledo A, Larralde C, Sciutto, E (2000) Taenia crassiceps cysticercosis: protective effect and immune response elicited by DNA immunization. J. Parasitol 86:67–74
  Google Scholar
• Freeman R (1962) Studies on the biology of Taenia crassiceps (Zender 1980) Rudolphi, 1810 (Cestoda). Can J Zool 40:969–990
  Google Scholar
• Hermanek J, Prokopic J (1989) Influence of thymic preparations of the result of experimental infection with Taenia crassiceps (Zeder, 1800) in ICR mice. Folia Parasitol 36:331–340
  PubMed Google Scholar
• Larralde C, Morales J, Terrazas LI, Govezensky T, Romano M (1995) Sex hormone changes induced by the parasite lead to feminization of the male host in murine Taenia crassiceps cysticercosis. J Steroid Biochem Mol Biol 52:575–580
  CAS PubMed Google Scholar
• Mosmann TR, Coffman RL (1989) Th1 and Th2 cells: different patterns of lymphokine secretion lead to different functional properties. Annu Rev Immunol 7:145–173
  CAS PubMed Google Scholar
• Mosmann TR, Sad S (1996) The expanding universe of T-cell subsets: Th1, Th2 and more. Immunol Today 17:138–146
  CAS PubMed Google Scholar
• Paul WE, Seder RA (1994) Lymphocyte responses and cytokines. Cell76: 241–251
• Robinson P, Atmar RL, Lewis DE, White AC (1997) Granuloma cytokines in murine cysticercosis. Infect Immun 65:2925–2931
  PubMed Google Scholar
• Spolski RJ, Corson J, Thomas PG, Kuhn RE (2000) Parasite-secreted products regulate the host response to larval Taenia crassiceps. Parasite Immunol 22:297–305
  Article PubMed Google Scholar
• Terrazas LI, Bojalil R, Morales J, Govezensk YT, Larralde C (1998) Shift from an early protective Th1 immune repsonse to a late permissive Th2 response in murine cysticercosis (Taenia crassiceps). J Parasitol 84:74–81
  CAS PubMed Google Scholar
• Terrazas LI, Cruz M, Rodriguez-Sosa M, Bojalil R, Garcia-Tamayo F, Larralde C (1999) Th1-type cytokines improve resistance to murine cysticercosis caused by Taenia crassiceps. Parasitol Res 85:135–141
  CAS PubMed Google Scholar
• Toenjes SA, Spolski RJ, Mooney KA, Kuhn RE (1999a) The systemic immune response of BALB/c mice infected with larval Taenia crassiceps is a mixed Th1/Th2-type response. Parasitology 118:623-633
  Article CAS PubMed Google Scholar
• Toenjes SA, Spolski RJ, Mooney KA, Kuhn RE (1999b) γδ T cells do not play a major role in controlling infection in experimental cysticercosis. Parasitology 119:413–418
  Article CAS PubMed Google Scholar
• Villa OF, Kuhn RE (1996) Mice infected with the larvae of Taenia crassiceps exhibit a Th2-like immune response with concomitant anergy and downregulation of Th1-asociated phenomena. Parasitology 122:561–570
  Google Scholar

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Acknowledgement

This research was supported by grant AI 35730 from the U.S. National Institutes of Health.

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1. Department of Biology, Wake Forest University, P.O. Box 7325, Winston-Salem, NC 27109, USA
   S. A. Toenjes & R. E. Kuhn

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1. S. A. Toenjes
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2. R. E. Kuhn
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Correspondence toR. E. Kuhn.

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Toenjes, S.A., Kuhn, R.E. The initial immune response during experimental cysticercosis is of the mixed Th1/Th2 type.Parasitol Res 89, 407–413 (2003). https://doi.org/10.1007/s00436-002-0788-z

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• Published: 17 December 2002
• Issue Date: March 2003
• DOI: https://doi.org/10.1007/s00436-002-0788-z

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