Genetic variability among Fasciola hepatica samples from different host species and geographical localities in Spain revealed by the novel SRAP marker (original) (raw)

Abstract

A collection of 483 samples representing Fasciola from six naturally infected host species and 16 localities in Spain, previously identified morphologically and genetically as Fasciola hepatica, was characterized by a novel genetic marker, namely sequence-related amplified polymorphism (SRAP), aiming to reveal genetic variability within F. hepatica in Spain. Visualization of amplification fragments was carried out on 6% denaturing polyacrylamide gels, followed by staining with 0.1% AgNO3 solution. Ten SRAP primer combinations were tested—six of them turned out to be polymorphic. Thirty-four representative F. hepatica samples from six host species and 16 geographical localities showed polymorphic banding patterns using SRAP primer combinations and were grouped into four major clusters using the unweighted pair-group method with arithmetic averages, indicating the existence of genetic variability within the examined F. hepatica samples. These four clusters were not related to particular host species and/or geographical origins of the samples. The results of the present study revealed that SRAP markers were useful in revealing sufficient polymorphism in F. hepatica samples from Spain and had implications for studying the population genetic structure of the Spanish F. hepatica. To our knowledge, this is the first application of SRAP marker to study genetic variation in parasites of human and animal health significance.

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Acknowledgements

Project support was provided in part by grants from the Program for Changjiang Scholars and Innovative Research Teams in Chinese Universities (Grant No. IRT0723) to XQZ, and a grant from Euphrates University-Syria to SA. Research activities in Spain were partially supported by the Plan Andaluz de Investigación (group RNM-118). The authors are indebted to Adolfo Paz-Silva (Departamento de Patología Animal, Parasitología y Enfermedades Parasitarias, Facultad de Veterinaria, Universidad de Santiago de Compostela), Ana L. García-Pérez and Ramón A. Juste (Sanidad Animal, Instituto Vasco de Investigación y Desarrollo Agrario, NEIKER), Margarita Buades (Head of the Sección de Mataderos, Islas Baleares) and Santiago Lavín (Universidad Autónoma de Barcelona) for providing F. hepatica samples and for their helpful comments. The experiments comply with the current laws of the countries in which the experiments were performed.

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Authors and Affiliations

1. College of Veterinary Medicine, South China Agricultural University, 483 Wushan Street, Tianhe District, Guangzhou, Guangdong Province, 510642, People’s Republic of China
   S. Alasaad, Q. Y. Li, R. Q. Lin & X. Q. Zhu
2. Departamento de Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén, Campus Las Lagunillas, s. n., 23071, Jaén, Spain
   S. Alasaad & J. M. Pérez
3. Parque Cinegético Experimental de Hosquillo, Servicio de Caza, Delegación Provincial de Medio Ambiente, C/ Colón nº 2, 16001, Cuenca, Spain
   P. Martín-Atance
4. Espacio Natural de Sierra Nevada, Carretera Antigua de Sierra Nevada, Km 7.5, 18071, Pinos Genil, Granada, Spain
   J. E. Granados
5. Departamento de Patología Animal, Parasitología y Enfermedades Parasitarias, Facultad de Veterinaria, Universidad de Santiago de Compostela, 27071, Lugo, Spain
   P. Díez-Baños

Authors

1. S. Alasaad
2. Q. Y. Li
3. R. Q. Lin
4. P. Martín-Atance
5. J. E. Granados
6. P. Díez-Baños
7. J. M. Pérez
8. X. Q. Zhu

Corresponding author

Correspondence toX. Q. Zhu.

Additional information

S. Alasaad and Q. Y. Li contributed equally to this work.

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Alasaad, S., Li, Q.Y., Lin, R.Q. et al. Genetic variability among Fasciola hepatica samples from different host species and geographical localities in Spain revealed by the novel SRAP marker.Parasitol Res 103, 181–186 (2008). https://doi.org/10.1007/s00436-008-0952-1

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• Received: 11 January 2008
• Accepted: 27 February 2008
• Published: 27 March 2008
• Issue date: June 2008
• DOI: https://doi.org/10.1007/s00436-008-0952-1

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