Cell fusion corrects the 4-nitroquinoline 1-oxide sensitivity of Werner syndrome fibroblast cell lines (original) (raw)
Abstract
We have shown that Werner syndrome (WRN) fibroblast cell lines are unusually sensitive to the DNA-damaging agent 4-nitroquinoline 1-oxide (4NQO), though not to gamma radiation or to hydrogen peroxide. The fusion of 4NQO-sensitive WRN and 4NQO-resistant control fibroblast cell lines generated proliferating WRN × control cell hybrids that expressed WRN protein and were 4NQO-resistant. These results establish the recessive nature of 4NQO sensitivity in WRN cell lines and provide a cellular assay for WRN protein function.
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Authors and Affiliations
- University of Washington, Department of Pathology, Box 357705, Seattle, WA 98195–7705, USA
P.R. Prince, C.E. Ogburn, M.J. Moser, G.M. Martin & R.J. Monnat Jr - University of Washington, Department of Biostatistics, Seattle, WA 98195, USA
M.J. Emond
Authors
- P.R. Prince
- C.E. Ogburn
- M.J. Moser
- M.J. Emond
- G.M. Martin
- R.J. Monnat Jr
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Prince, P., Ogburn, C., Moser, M. et al. Cell fusion corrects the 4-nitroquinoline 1-oxide sensitivity of Werner syndrome fibroblast cell lines.Hum Genet 105, 132–138 (1999). https://doi.org/10.1007/s004399900078
- Received: 01 March 1999
- Accepted: 30 April 1999
- Published: 28 June 1999
- Issue date: August 1999
- DOI: https://doi.org/10.1007/s004399900078