Isolation and phylogenetic characterization of bat astroviruses in southern China (original) (raw)

Abstract

Astroviruses are associated with acute gastroenteritis of human and many animal species. Recently, two studies have reported that novel astroviruses were found in bats. In order to extensively understand the genetic and phylogenetic characterization of bat astroviruses, we tested fecal samples of nine bat species that were collected at four natural habitats in three areas of southern China. The geographic distributions of the bats involved differed from previous reports. Three out of nine species of bats were observed to harbor astroviruses. These included Miniopterus schreibersii, Scotophilus kuhlii, and Rousettus leschenaultia. Phylogenetic analysis based on amino acid sequences of partial ORFs of astroviruses revealed that the bat astroviruses are not only divergent from previously described human and other animal astroviruses but also show remarkable diversity among themselves. However, five bat astroviruses were phylogenetically related to mink astrovirus, ovine astrovirus, and the recently discovered human astroviruses VA1, VA2, and VA3. The results indicate that astroviruses may have adapted to the Chiroptera, and bats may transmit astroviruses to humans and other animals, or vice versa.

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References

  1. Anderson EJ (2010) Prevention and treatment of viral diarrhea in pediatrics. Expert Rev Anti Infect Ther 8:205–217
    Article PubMed Google Scholar
  2. Baxendale W, Mebatsion T (2004) The isolation and characterisation of astroviruses from chickens. Avian Pathol 33:364–370
    Article PubMed CAS Google Scholar
  3. Calisher CH, Childs JE, Field HE, Holmes KV, Schountz T (2006) Bats: important reservoir hosts of emerging viruses. Clin Microbiol Rev 19:531–545
    Article PubMed Google Scholar
  4. Chu DK, Poon LL, Guan Y, Peiris JS (2008) Novel astroviruses in insectivorous bats. J Virol 82:9107–9114
    Article PubMed CAS Google Scholar
  5. Chu DK, Chin AW, Smith GJ, Chan KH, Guan Y, Peiris JS, Poon LL (2010) Detection of novel astroviruses in urban brown rats and previously known astroviruses in humans. J Gen Virol 91:2457–2462
    Article PubMed CAS Google Scholar
  6. Dennehy PH, Nelson SM, Spangenberger S, Noel JS, Monroe SS, Glass RI (2001) A prospective case–control study of the role of astrovirus in acute diarrhea among hospitalized young children. J Infect Dis 184:10–15
    Article PubMed CAS Google Scholar
  7. Finkbeiner SR, Kirkwood CD, Wang D (2008) Complete genome sequence of a highly divergent astrovirus isolated from a child with acute diarrhea. Virol J 5:117
    PubMed Google Scholar
  8. Finkbeiner SR, Li Y, Ruone S, Conrardy C, Gregoricus N, Toney D, Virgin HW, Anderson LJ, Vinje J, Wang D, Tong S (2009) Identification of a novel astrovirus (astrovirus VA1) associated with an outbreak of acute gastroenteritis. J Virol 83:10836–10839
    Article PubMed CAS Google Scholar
  9. Glass RI, Noel J, Mitchell D, Herrmann JE, Blacklow NR, Pickering LK, Dennehy P, Ruiz-Palacios G, de Guerrero ML, Monroe SS (1996) The changing epidemiology of astrovirus-associated gastroenteritis: a review. Arch Virol Suppl 12:287–300
    PubMed CAS Google Scholar
  10. Jiang B, Monroe SS, Koonin EV, Stine SE, Glass RI (1993) RNA sequence of astrovirus: distinctive genomic organization and a putative retrovirus-like ribosomal frame shifting signal that directs the viral replicase synthesis. Proc Natl Acad Sci USA 90:10539–10543
    Article PubMed CAS Google Scholar
  11. Koci MD, Schultz-Cherry S (2002) Avian astroviruses. Avian Pathol 31:213–227
    Article PubMed CAS Google Scholar
  12. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23:2947–2948
    Article PubMed CAS Google Scholar
  13. Lee TW, Kurtz JB (1994) Prevalence of human astrovirus serotypes in the Oxford region 1976–92, with evidence for two new serotypes. Epidemiol Infect 112:187–193
    Article PubMed CAS Google Scholar
  14. Poon LL, Chu DK, Chan KH, Wong OK, Ellis TM, Leung YH, Lau SK, Woo PC, Suen KY, Yuen KY, Guan Y, Peiris JS (2005) Identification of a novel coronavirus in bats. J Virol 79:2001–2009
    Article PubMed CAS Google Scholar
  15. Rivera R, Nollens HH, Venn-Watson S, Gulland FM, Wellehan JJ (2010) Characterization of phylogenetically diverse astroviruses of marine mammals. J Gen Virol 91:166–173
    Article PubMed CAS Google Scholar
  16. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599
    Article PubMed CAS Google Scholar
  17. Taylor MB, Walter J, Berke T, Cubitt WD, Mitchell DK, Matson DO (2001) Characterisation of a South African human astrovirus as type 8 by antigenic and genetic analyses. J Med Virol 64:256–261
    Article PubMed CAS Google Scholar
  18. Toffan A, Jonassen CM, De Battisti C, Schiavon E, Kofstad T, Capua I, Cattoli G (2009) Genetic characterization of a new astrovirus detected in dogs suffering from diarrhoea. Vet Microbiol 139:147–152
    Article PubMed CAS Google Scholar
  19. Zhu HC, Chu DK, Liu W, Dong BQ, Zhang SY, Zhang JX, Li LF, Vijaykrishna D, Smith GJ, Chen HL, Poon LL, Peiris JS, Guan Y (2009) Detection of diverse astroviruses from bats in China. J Gen Virol 90:883–887
    Article PubMed CAS Google Scholar

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Acknowledgments

We sincerely thank Dr. Poon for helpful suggestions and discussions. This work was supported by National Natural Science Foundation of China (Grant No. 30571610), National Natural Science Foundation of Guangdong Province (Grant No. 8151051501000056, 8151009101000005), and Guangdong Province “211 Project”.

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Authors and Affiliations

  1. Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, 1838 Guangzhou North Road, Guangzhou, 510515, Guangdong, China
    Jianpeng Xiao, Jiandong Li, Guifang Hu, Yixiong Chen, Zhiyong Chen, Yuxue Liao, Jie Zhou, Xuemei Ke, Lizhen Ma, Shan Liu, Junhua Zhou, Yingchun Dai, Hongbiao Chen, Shouyi Yu & Qing Chen
  2. Key Laboratory of Prevention and Control of Emerging Infectious Diseases of Guangdong Higher Education Institutes, Southern Medical University, Guangzhou, 510515, China
    Jianpeng Xiao, Lizhen Ma, Shan Liu & Qing Chen
  3. College of Life Science, Hainan Normal University, Haikou, 571158, China
    Zhong Chen
  4. College of Life Science, Guangzhou University, Guangzhou, 510006, China
    Yi Wu

Authors

  1. Jianpeng Xiao
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  2. Jiandong Li
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  3. Guifang Hu
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  4. Zhong Chen
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  5. Yi Wu
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  6. Yixiong Chen
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  7. Zhiyong Chen
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  8. Yuxue Liao
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  9. Jie Zhou
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  10. Xuemei Ke
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  11. Lizhen Ma
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  12. Shan Liu
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  13. Junhua Zhou
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  14. Yingchun Dai
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  15. Hongbiao Chen
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  16. Shouyi Yu
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  17. Qing Chen
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Corresponding author

Correspondence toQing Chen.

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Xiao, J., Li, J., Hu, G. et al. Isolation and phylogenetic characterization of bat astroviruses in southern China.Arch Virol 156, 1415–1423 (2011). https://doi.org/10.1007/s00705-011-1011-2

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