Occurrence and biochemical characteristics of cestode lymphocyte mitogens | Parasitology | Cambridge Core (original) (raw)

Summary

The cestodes Echinococcus granulosus, Taenia multiceps, T. pisiformis, T. hydatigena, Hymenolepis diminuta, Moniezia expansa and Anoplocephala perfoliata all produced substances that stimulated thymidine incorporation by whole blood lymphocyte cultures. This mitogenic activity was demonstrated in metacestode cyst fluids, live protoscoleces and scoleces, parasite culture supernatants, and extracts of adult parasites. T. multiceps metacestode cyst fluid mitogen adhered to, but would not pass through, cellulose dialysis tubing. This adherence was reduced or prevented by D-glucose and by proteins. The mitogen was weakly anionic. With Sephadex G75 gel filtration, its elution volume was greatly decreased when the elution buffer contained 0·5 M D-glucose. Mitogenic activity was protease resistant and could be separated by gel filtration from all the cyst fluid proteins.

References

Dixon, J. B., Jenkins, P. & Allan, D. (1982). Immune recognition of Echinococcus granulosus. 1. Parasite activated primary transformation by normal murine lymph node cells. Parasite Immunology 4, 33–45.CrossRefGoogle ScholarPubMed

Goldstein, I. J. & Hayes, C. E. (1978). The lectins: carbohydrate binding proteins of plants and animals. In Advances in Carbohydrate Chemistry and Biochemistry, vol. 35, pp. 128–270, (ed. Stuart, R. and Horton, D.). New York: Academic Press.Google Scholar

Judson, D. G. & Dixon, J. B. (1985). Depression of lymphocyte reactivity by granulocytes in equine whole blood culture. Veterinary Immunology and Immunopathology 8, 289–95.Google Scholar

Judson, D. G., Dixon, J. B., Skerritt, G. C. & Stallbaumer, M. (1984). Mitogenic effect of Coenurus cerebralis cyst fluid. Research in Veterinary Science 37, 128.CrossRefGoogle ScholarPubMed

Laemmli, U. K. & Favre, M. (1973). Maturation of the head of the bacteriophage T4. 1. DNA packing events. Journal of Molecular Biology 80, 575–99.CrossRefGoogle Scholar

Sealey, M., Ramos, C., Williams, K. & Ortiz-Ortiz, L. (1981). Taenia solium: mitogenic effect of larval extracts on murine B lymphocytes. Parasite Immunology 3, 299–307.CrossRefGoogle ScholarPubMed