Opisthorchis viverrini: relationships between egg counts, worms recovered and antibody levels within an endemic community in Northeast Thailand | Parasitology | Cambridge Core (original) (raw)

Summary

Three techniques for estimating the intensity of Opisthorchis viverrini infection in individuals from a Northeast That community are compared. Egg counts were determined using a quantitative formalin/ethyl acetate technique, worm burdens were estimated by expulsion chemotherapy and antibody levels were measured by ELISA. Log-transformed worm and egg counts were closely correlated (r = 0·80), suggesting that both measurements provide good assessments of relative intensity of infection. However, no Opisthorchis worms were recovered from 34 people with high egg counts; probably due to problems with the expulsion technique in some individuals. Examination of egg production per fluke indicated that each fluke contributed an average of 180 eggs per gram (epg) of faeces and fecundity was negatively associated with total worm burden. Serum IgG levels correlated significantly with Opisthorchis egg count (r = 0·61) at two independent assessments. Although significant associations were observed between antibody levels and echinostome infection, analysis suggested that these reflected independent associations between these two variables and Opisthorchis infection and age. We conclude that all three measurements are useful for epidemiological studies.

References

Bunnag, D. & Harinasuta, T. (1981). Studies on the chemotherapy of human opisthorchiasis – III. Minimum effective dose of praziquantel South east Asian Journal of Tropical Medicine and Public Health 12, 413–17.Google Scholar PubMed

Elkins, D. B., Haswell-ELKINS, M. & Anderson, R. M. (1986). The epidemiology and control of intestinal helminths in the Pulicat Lake region of Southern India. I. Study design and pre- and post-treatment observations on Ascaris lumbricoides infection. Transactions of the Royal Society of Tropical Medicine and Hygiene 80, 774–92.CrossRef Google Scholar

Elkins, D. B., Haswell-ELKINS, M. R., Mairiang, E., Mairiang, P., Sithithaworn, P., Kaewkes, S., Bhuddhisawasdi, V. & Uttaravichien, T. (1990). A high frequency of hepatobiliary disease and suspected cholangiocarcinoma associated with heavy Opisthorchis viverrini infection in a small community in Northeast Thailand. Transactions of the Royal Society of Tropical Medicine and Hygiene 84, 715–19.CrossRef Google Scholar

Feldheim, W. & Knoblock, J. (1982). Serodiagnosis of Opisthorchis viverrini infestation by an enzyme immunoassay. Tropenmedizin und Parasitologie 33, 8–10.Google Scholar

Flavell, D. J., Flavell, S. U. & Field, G. F. (1983). Opisthorchis viverrini: the relationship between egg production, worm size and intensity of infection. Transactions of the Royal Society of Tropical Medicine and Hygiene 77, 538–45.CrossRef Google Scholar PubMed

Flavell, D. J., Pattanapanyasat, K. & Flavell, S. U. (1980). Opisthorchis viverrini: partial success in adoptively transferring immunity with spleen cells and serum in the hamster. Journal of Helminthology 54, 191–7.CrossRef Google Scholar PubMed

Hall, A. (1981). Aspects of parasitic infection and host nutrition. Ph.D. thesis, University of Cambridge.Google Scholar

Harinasuta, T., Riganti, M. & Bunnag, D. (1984). Opisthorchis viverrini infection: pathogenesis and clinical features. Drug Research 34, 1167–9.Google Scholar PubMed

Hou, P. C. (1956). The relationship between primary carcinoma of the liver and infestation with Clonorchis sinensis. Journal of Pathology and Bacteriology 72, 239–46.Google Scholar PubMed

Kurathong, S., Brockelman, W. Y., Lerdverasirikul, P., Wongpaitoon, V., Kanjanapitak, A., Varavithya, W., Upatham, E. S. & Viyanant, V. (1984). Consistency of fecal egg output in patients with opisthorchiasis viverrini. American Journal of Tropical Medicine and Hygiene 33, 73–5.CrossRef Google Scholar

Preuksaraj, S. (1984). Public health aspects of opisthorchiasis in Thailand. Drug Research 34, 1119–20.Google Scholar PubMed

Radomyos, P., Bunnag, D. & Harinasuta, T. (1984). Worms recovered in stools following praziquantel treatment. Drug Research 34, 1215.Google Scholar

Ramsey, R. J., Sithithaworn, P., Prociv, P., Moorhouse, D. E. & Methaphat, C. (1989). Density dependent fecundity of Opisthorchis viverrini in humans, based on faecal recovery of flukes. Transactions of the Royal Society of Tropical Medicine and Hygiene 83, 241–2.CrossRef Google Scholar

Sithithaworn, P., Tesana, S., Pipitgool, V., Kaewkes, S., Pairojkul, C., Sripa, B., Paupairoj, A. & Thaiklar, K. (1991). Relationship between faecal egg count and worm burden of Opisthorchis viverrini in human autopsy cases. Parasitology 102, 277–81.CrossRef Google Scholar PubMed

Sonakul, D., Koompirochana, C., Chinda, K. & Stitnimakarn, T. (1978). Hepatic carcinoma with opisthorchiasis. Southeast Asian Journal of Tropical Medicine and Public Health 9, 215–19.Google Scholar PubMed

Srivatanakul, P., Viyanant, V., Kurathong, S. & Tiwawech, D. (1985). Enzyme-linked immunosorbent assay for detection of Opisthorchis viverrini infection. Southeast Asian Journal of Tropical Medicine and Public Health 16, 234–9.Google Scholar PubMed

Upatham, E. S., Viyanant, V., Kurathong, S., Rojborwonwitaya, J., Brockelman, W. Y., Ardsungnoen, S., Lee, P. & Vajrasthira, S. (1984). Relationship between prevalence and intensity of Opisthorchis viverrini infection, and clinical symptoms and signs in a rural community in northeast Thailand. Bulletin of the World Health Organization 62, 451–61.Google Scholar

Wongratanacheewin, S., Bunnag, D., Vaeusorn, N. & Sirisinha, S. (1988). Characterization of humoral immune response in the serum and bile of patients with opisthorchiasis and its application in immunodiagnosis. American Journal of Tropical Medicine and Hygiene 38, 356–62.CrossRef Google Scholar PubMed