Rapid increase of an immediate early gene messenger RNA in hippocampal neurons by synaptic NMDA receptor activation (original) (raw)
- Letter
- Published: 10 August 1989
Nature volume 340, pages 474–476 (1989)Cite this article
- 1888 Accesses
- 933 Citations
- 2 Altmetric
- Metrics details
Abstract
RECENT studies in invertebrates indicate that a rapid genomic response to neuronal stimulation has a critical role in long-term changes in synaptic efficacy1. Because several of the genes (immedi-ately early genes; lEGs) that respond rapidly to growth factor stimulation of vertebrate cells _in vitro_2-7 are also activated by neuronal stimulation _in vivo_8-13, attention has focused on the possibility that they play a part in synaptic plasticity in vertebrate nervous systems. Four lEGs thought to encode transcription fac-tors, _zif/268_5 (also termed _Egr-l_l4, _NGFI-A_15, _Krox24_16), _C-fOS_17, c-_jun_18, and _jun-B_7 are rapidly induced in the brain by seizure activity8,11,13, and we have now studied the induction of these genes in a well-characterized model of synaptic plasticity in the verte-brate brain—-long-term potentiation (LTP) of the perforant path-granule cell (pp-gc) synapse _in vivo_19. We found that high-frequency (but not low-frequency) stimulation of the pp-gc synapse markedly increases zif/268 messenger RNA (mRNA) levels in the ipsilateral granule cell neurons; mRNA of c-fos, c-jun and jun-B is less consistently increased. The stimulus frequency and intensity required to increase zif/268 mRNA levels are similar to those required to induce LTP, which is also seen only ipsilaterally, and both responses are blocked by NMDA-receptor antagonists as well as by convergent synaptic inhibitory inputs already known to block LTP20. Accordingly, zif/268 mRNA levels and LTP seem to be regulated by similar synaptic mechanisms.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Additional access options:
Similar content being viewed by others
References
- Montarolo, P. G. et al. Science 234, 1249–1254 (1986).
Article ADS CAS Google Scholar - Cochran, B. H., Keffel, A. C. & Stiles, C. D. Cell 33, 939–947 (1983).
Article CAS Google Scholar - Lau, L. F. & Nathans, D. EMBO J. 4, 3145–3151 (1985).
Article CAS Google Scholar - Greenberg, M. E., Ziff, E. B. & Greene, L. A. Science 234, 80–83 (1986).
Article ADS CAS Google Scholar - Christy, B. A., Lau, L. F. & Nathans, D. Proc. natn. Acad. Sci. U.S.A. 85, 7857–7861 (1988).
Article ADS CAS Google Scholar - Ryder, K. & Nathans, D. Proc. natn. Acad. Sci. U.S.A. 85, 8464–8467 (1988).
Article ADS CAS Google Scholar - Ryder, L., Lau, L. F. & Nathans, D. Proc. natn. Acad. Sci. U.S.A. 85, 1487–1491 (1988).
Article ADS CAS Google Scholar - Morgan, J. I., Cohen, D. R., Hempstead, J. L. & Curran, T. Science 237, 192–197 (1987).
Article ADS CAS Google Scholar - Sagar, S. M., Sharp, F. R. & Curran, T. Science 240, 1328–1331 (1988).
Article ADS CAS Google Scholar - Hunt, S. P., Pini, A. & Evan, G. Nature 328, 632–634 (1987).
Article ADS CAS Google Scholar - Dragunow, M. & Robertson, H. A. Nature 329, 441–442 (1987).
Article ADS CAS Google Scholar - Douglas, R. M., Dragunow, M. & Robertson, H. A. Molec. Brain Res. 4, 259–262 (1988).
Article Google Scholar - Saffen, D. W. et al. Proc. natn. Acad. Sci. U.S.A. 85, 7795–7799 (1988).
Article ADS CAS Google Scholar - Sukhatme, V. P. et al. Cell 53, 37–43 (1988).
Article CAS Google Scholar - Milbrandt, J. Science 238, 797–799 (1987).
Article ADS CAS Google Scholar - Lemaire, P., Revelant, O., Bravo, R. & Charnay, P. Proc. natn. Acad. Sci. U.S.A. 85, 4691–4695 (1988).
Article ADS CAS Google Scholar - Franza, B. R. Jr, Rauscher, F. J. III, Josephs, S. F. & Curran, T. Science 239, 1150–1153 (1988).
Article ADS CAS Google Scholar - Rauscher, F. J. III et al. Science 240, 1010–1016 (1988).
Article ADS CAS Google Scholar - Bliss, T. V. P. & Lynch, M. A. in Long-term Potentiation: From Biophysics to Behavior, 3–72 (Alan R. Liss, Inc., 1988).
Google Scholar - Douglas, R. M., Goddard, G. V. & Riives, M. Brain Res. 240, 259–272 (1982).
Article CAS Google Scholar - McNaughton, B. L., Douglas, R. M. & Goddard, G. V. Brain Res. 157, 277–293 (1978).
Article CAS Google Scholar - Bliss, T. V. P., Goddard, G. V. & Riives, M. J. Physiol., Lond. 334, 475–491 (1983).
Article CAS Google Scholar - Goldowitz, D. W., White, F., Steward, O., Cotman, C. W. & Lynch, G. S. Expl Neurol. 47, 433–441 (1975).
Article CAS Google Scholar - Lau, L. F. & Nathans, D. Proc. natn. Acad. Sci. U.S.A. 84, 1182–1186 (1987).
Article ADS CAS Google Scholar - Errington, M. L., Lynch, M. A. & Bliss, T. V. P. Neuroscience 20, 279–284 (1987).
Article CAS Google Scholar - Nicoll, R. A., Kauer, J. A. & Malenka, R. C. Neuron 1, 97–103 (1988).
Article CAS Google Scholar - Wong, E. H. F. et al. Proc. natn. Acad. Sci. U.S.A. 83, 7104–7108 (1986).
Article ADS CAS Google Scholar - Abraham, W. C. & Mason, S. E. Brain Res. 462, 40–46 (1988).
Article CAS Google Scholar - Murphy, D. E., Hutchinson, A. J., Hurt, S. D., Williams, M. & Sills, M. A. Br. J. Pharmac. 95, 932–938 (1988).
Article CAS Google Scholar - Lodge, D. et al. Br. J. Pharmac. 95, 957–965 (1988).
Article CAS Google Scholar
Author information
Authors and Affiliations
- Departments of Neuroscience, Neurology, Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland, 21205, USA
Andrew J. Cole, David W. Saffen, Jay M. Baraban & Paul F. Worley
Authors
- Andrew J. Cole
You can also search for this author inPubMed Google Scholar - David W. Saffen
You can also search for this author inPubMed Google Scholar - Jay M. Baraban
You can also search for this author inPubMed Google Scholar - Paul F. Worley
You can also search for this author inPubMed Google Scholar
Rights and permissions
About this article
Cite this article
Cole, A., Saffen, D., Baraban, J. et al. Rapid increase of an immediate early gene messenger RNA in hippocampal neurons by synaptic NMDA receptor activation.Nature 340, 474–476 (1989). https://doi.org/10.1038/340474a0
- Received: 07 April 1989
- Accepted: 07 July 1989
- Issue Date: 10 August 1989
- DOI: https://doi.org/10.1038/340474a0