Speciation of Selenoamino Acids and Organoselenium Compounds inSelenium-enriched Yeast Using High-performance LiquidChromatography–Inductively Coupled Plasma Mass Spectrometry (original) (raw)

As part of an ongoing study to identify selenium compounds with cancer chemopreventive activity, selenium-enriched yeast was analyzed by HPLC–ICP-MS. More than twenty selenium-containing species were found in hot water and enzymatic hydrolysis extracts of the yeast. Trifluoroacetic acid was used as an ion-pairing agent in a water–methanol mobile phase with reversed-phase chromatography on an octylsilane stationary phase. The presence of selenocystine, selenomethionine and methylselenocysteine was confirmed by comparative retention of standards. The column efficiency was 8500 theoretical plates and the mobile phase was compatible with standard ICP-MS operating conditions.

You have access to this article

Please wait while we load your content... Something went wrong. Try again?