Structure of tbe gene for Xenopus transcription factor TMIIIA (original) (raw)

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Department of Genetics, Stanford University School of Medicine

Stanford, CA 94305, USA

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Department of Genetics, Stanford University School of Medicine

Stanford, CA 94305, USA

Search for other works by this author on:

Department of Genetics, Stanford University School of Medicine

Stanford, CA 94305, USA

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Received:

02 January 1986

Accepted:

04 February 1986

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J.Yun Tso, David J.Van Den Berg, Laurence Jay Korn, Structure of tbe gene for Xenopus transcription factor TMIIIA , Nucleic Acids Research, Volume 14, Issue 5, 11 March 1986, Pages 2187–2200, https://doi.org/10.1093/nar/14.5.2187
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Abstract

The eucaryotic transcription factor TFIIIA ia required for 58 RNA transcription in Xenopus, and changes in the level of TFIIIA have been implicated in the differential expression of 58 RNA genes. In this paper, we report the isolation and sequencing of the X. laevis TFIIIA gene. The gene is approximately 11 kb in length and conaiats of 9 coding segaenta separated by 8 introna. A sequence of 30 aaino acid reaiduea is known to repeat imperfectly 9 times in tandem within the TFIIIA protein, and Miller et al. (EMBO J. 4, 1609–1614, 1985) proposed that TFIIIA evolved by duplication of a primordial 30 amino acid residue unit. Our results from DHA sequence analysis support their proposal by showing that some of the exon-intron boundaries correspond closely to the repeating unit. We also found that the 5' flanking sequence of the TTIIIA gene contains a TATA box (TATATAA) at position −32 and a CAAT box (GCCAATCC) at poaition -96 and that the aite of polyadenylation ia 255 residuea 3' of the stop codon. Finally, we have shown that the coding sequence of the TFIIIA gene is significantly polymorphic.

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