Preservation of C. elegans Tissue Via High-Pressure Freezing and Freeze-Substitution for Ultrastructural Analysis and Immunocytochemistry (original) (raw)
References
White, J. G., Southgate, E., Thomson, J. N., and Brenner, S. (1986) The structure of the nervous system of the nematode Caenorhabditis elegans. Phil. Trans. R. Soc. Lond.B314, 1–340. Google Scholar
Albertson, D. G. and Thomson, J. N. (1976) The pharynx of Caenorhabditis elegans. Philos. Trans. R. Soc. Lond. B. Biol. Sci.275, 299–325. ArticlePubMedCAS Google Scholar
Epstein, H. F., Waterston, R. H., and Brenner, S. (1974) A mutant affecting the heavy chain of myosin in Caenorhabditis elegans. J. Mol. Biol.90, 291–300. ArticlePubMedCAS Google Scholar
Ward, S., Thomson, N., White, J. G., and Brenner, S. (1975) Electron microscopical reconstruction of the anterior sensory anatomy of the nematode Caenorhabditis elegans. J. Comp. Neurol.160, 313–337. ArticlePubMedCAS Google Scholar
Waterston, R. H., Epstein, H. F., and Brenner, S. (1974) Paramyosin of Caenorhabditis elegans. J. Mol. Biol.90, 285–290. ArticlePubMedCAS Google Scholar
Bosher, J. M., Hahn, B. S., Legouis, R., et al. (2003) The Caenorhabditis elegans vab-10 spectraplakin isoforms protect the epidermis against internal and external forces. J. Cell Biol.161, 757–768. ArticlePubMedCAS Google Scholar
Dernburg, A. F., McDonald, K., Moulder, G., Barstead, R., Dresser, M., and Villeneuve, A. M. (1998) Meiotic recombination in C. elegans initiates by a conserved mechanism and is dispensable for homologous chromosome synapsis. Cell94, 387–398. ArticlePubMedCAS Google Scholar
Favre, R., Cermola, M., Paiva Nunes, C., Hermann, R., Muller, M., and Bazzicalupo, P. (1998) Immuno-cross-reactivity of CUT-1 and cuticlin epitopes between Ascaris lumbricoides, Caenorhabditis elegans, and Heterorhabditis. J. Strut. Biol.123, 1–7. Google Scholar
Favre, R., Hermann, R., Cermola, M., Hohenberg, H., Muller, M., and Bazzicalupo, P. (1995) Immuno-gold-labelling of CUT-1, CUT-2 and cuticlin epitopes in Caenorhabditis elegans and Heterorhabditis sp. processed by high pressure freezing and freeze-substitution. J. Submicrosc. Cytol. Pathol.27, 341–347. PubMedCAS Google Scholar
Howe, M., McDonald, K. L., Albertson, D. G., and Meyer, B. J. (2001) HIM-10 Is required for kinetochore structure and function on Caenorhabditis elegans holocentric chromosomes. J. Cell Biol.153, 1227–1238. ArticlePubMedCAS Google Scholar
Kirkham, M., Muller-Reichert, T., Oegema, K., Grill, S., and Hyman, A. A. (2003) SAS-4 is a C. elegans centriolar protein that controls centrosome size. Cell.112, 575–587. ArticlePubMedCAS Google Scholar
Koppen, M., Simske, J. S., Sims, P. A., et al. (2001) Cooperative regulation of AJM-1 controls junctional integrity in Caenorhabditis elegans epithelia. Nat. Cell Biol.3, 983–991. ArticlePubMedCAS Google Scholar
Leapman, R. D., Kocsis, E., Zhang, G., Talbot, T. L., and Laquerriere, P. (2004) Three-dimensional distributions of elements in biological samples by energy-filtered electron tomography. Ultramicroscopy100, 115–125. ArticlePubMedCAS Google Scholar
MacQueen, A. J., Colaiacovo, M. P., McDonald, K., and Villeneuve, A. M. (2002) Synapsis-dependent and-independent mechanisms stabilize homolog pairing during meiotic prophase in C. elegans. Genes Dev.16, 2428–2442. ArticlePubMedCAS Google Scholar
Rappleye, C. A., Paredez, A. R., Smith, C. W., McDonald, K. L., and Aroian, R. V. (1999) The coronin-like protein POD-1 is required for anterior-posterior axis formation and cellular architecture in the nematode Caenorhabditis elegans. Genes Dev.13, 2838–2851. ArticlePubMedCAS Google Scholar
Rolls, M. M., Hall, D. H., Victor, M., Stelzer, E. H., and Rapoport, T. A. (2002) Targeting of rough endoplasmic reticulum membrane proteins and ribosomes in invertebrate neurons. Mol. Biol. Cell.13, 1778–1791. ArticlePubMedCAS Google Scholar
Rostaing, P., Weimer, R. M., Jorgensen, E. M., Triller, A., and Bessereau, J. L. (2004) Preservation of immunoreactivity and fine structure of adult C. Elegans tissues using high-pressure freezing. J. Histochem. Cytochem.52, 1–12. PubMedCAS Google Scholar
Williams-Masson, E. M., Heid, P. J., Lavin, C. A., and Hardin, J. (1998) The cellular mechanism of epithelial rearrangement during morphogenesis of the Caenorhabditis elegans dorsal hypodermis. Dev. Biol.204, 263–276. ArticlePubMedCAS Google Scholar
Yeh, E., Kawano, T., Weimer, R. M., Bessereau, J. L., and Zhen, M. (2005) Identification of genes involved in synaptogenesis using a fluorescent active zone marker in Caenorhabditis elegans. J. Neurosci.25, 3833–3841. ArticlePubMedCAS Google Scholar
Gilkey, J. C. and Staehelin, L. A. (1986) Advances in ultrarapid freezing for the preservation of cellular ultrastructure. J. Electron Micros. Tech.3, 177–210. Article Google Scholar
Brüggeller, P. and Mayer, E. (1980) Complete vitrification in pure liquid water and dilute aqueous solutions. Nature288, 569–571. Article Google Scholar
Dubochet, J. and McDowall, A. W. (1981) Vitrification of pure water for electron microscopy. J. Microsc.124, RP3–RP4. Google Scholar
Al-Amoudi, A., Chang, J. J., Leforestier, A., et al (2004) Cryo-electron microscopy of vitreous sections. Embo. J.23, 3583–3588. ArticlePubMedCAS Google Scholar
Walther, P. (2003) Recent progress in freeze-fracturing of high-pressure frozen samples. J. Microsc.212, 34–43. ArticlePubMedCAS Google Scholar
McDonald, K. (1997) Freeze substitution. In: Cells: A Laboratory Manual, (Spector, D. L., Goldman, R. D., and Leinwand, L. A., eds.), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 130.1–130.7. Google Scholar
Steinbrecht, R. A. and Muller, M. (1987) Freeze-substitution and freeze-drying. In: Cryotechniques in Biological Electron Microscopy, (Steinbrecht, S. R. and Zierold, K., eds.), Springer-Verlag, Berlin, 149–172. Google Scholar
Erickson, P. A., Geoffrey, P. L., and Fisher, S. K. (1993) Postembeding immunocytochemical techniques for light and electron microscopy. In: Methods in Cell Biology: Antibodies in Cell Biology, (Asai, D. J., ed.), Academic Press, Inc, San Diego, CA, pp. 283–310. Google Scholar
Brenner, S. (1974) The genetics of Caenorhabditis elegans. Genetics77, 71–94. PubMedCAS Google Scholar
Hayat, M. A. (1989) Colloidal Gold: Principles, Methods, and Applications, Academic Press, San Diego, CA. Google Scholar
Verkleij, A. J. and Leunissen, J. L. M. (1989) Immuno-Gold Labeling in Cell Biology, CRC Press, Boca Raton, FL. Google Scholar
McDonald, K. (1999) High-pressure freezing for preservation of high resolution fine structure and antigenicity for immunolabeling. Methods Mol. Biol.117, 77–97. ArticlePubMedCAS Google Scholar
Walther, P. and Ziegler, A. (2002) Freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water. J. Microsc.208, 3–10. ArticlePubMedCAS Google Scholar
Giddings, T. H. (2003) Freeze-substitution protocols for improved visualization of membranes in high-pressure frozen samples. J. Microsc.212, 53–61. ArticlePubMedCAS Google Scholar
Zhen, M. and Jin, Y. (1999) The liprin protein SYD-2 regulates the differentiation of presynaptic termini in C. elegans. Nature401, 371–375. PubMedCAS Google Scholar
Li, X. and Sze, H. (1999) A 100 kDa polypeptide associates with the V0 membrane sector but not with the active oat vacuolar H(+)-ATPase, suggesting a role in assembly. Plant J.17, 19–30. ArticlePubMed Google Scholar