Nigel Lambert | University of East Anglia (original) (raw)

Papers by Nigel Lambert

Research paper thumbnail of Norleg

Nutrition & Food Science, 1991

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Research paper thumbnail of New Looks at Neglected Legumes

Nutrition & Food Science, 1989

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Research paper thumbnail of Modified procedure for rapid purification of protein disulphide-isomerase from bovine liver

Biochemical Society Transactions, 1982

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Research paper thumbnail of Chemical modification of essential residues in purified rat liver protein disulphide-isomerase

Biochemical Society Transactions, 1984

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Research paper thumbnail of Assessing patients' preferences for treatments for angina using a modified repertory grid method

Soc Sci Med 60 2585 2595, Jun 1, 2005

A current popular theme in medicine concerns whether and how patients should be involved in treat... more A current popular theme in medicine concerns whether and how patients should be involved in treatment choice. Assuming patient involvement is desirable, how should one go about eliciting preferences? A variety of quantitative and qualitative methods exist that may be used for this purpose, one of which is the repertory grid method. This method involves eliciting constructs (reasons) for preferences through comparing sets of three options. This method allows the structured elicitation of the reasons behind individual preferences, but also, when used with generalised procrustes analysis (GPA), allows aggregation of individual data to reveal general preference patterns. In this study the repertory grid method was used to examine patient preferences for angina treatments with the goal of, first, gaining some understanding of general patterns of patient preference, and second, examining the likely utility of the technique in this setting. A sample of 21 patients with mild and stable angina from two general practices in Norfolk, UK was interviewed using the repertory grid method to elicit the constructs underlying their preferences amongst seven angina treatments (including 'no treatment'). Individualised questionnaires were then produced and sent to the patients for self-completion, which required rating the extent to which each construct was relevant for each treatment (scored on visual analogue rating scales). Analysis of the ratings, using GPA, showed that the constructs clustered around two dimensions: 'some treatment' versus 'no treatment', and drug treatment versus surgical treatment. While some treatment was generally preferred to no treatment, individuals varied in preference for drug treatments or surgical treatments. Although the latter were generally perceived as 'effective' they were also perceived, for example, as 'invasive', 'frightening', related to 'negative experiences', and being more appropriate for when symptoms are severe ('proportionate'). We consider the implications of these results for involving patients in choosing amongst treatments.

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Research paper thumbnail of Understanding patients' preferences for treatment: the need for innovative methodologies

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Research paper thumbnail of A study of patient expectations in a Norfolk general practice: Patient expectations in a Norfolk general practice

Health Expectations an International Journal of Public Participation in Health Care and Health Policy, Sep 1, 2010

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Research paper thumbnail of Characterisation and crystallisation of an 11S seed storage globulin from coconut (Cocos nucifera)

Food Chemistry, 1990

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Research paper thumbnail of Smoking cessation and vegetable consumption : reducing cancer through communication?

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Research paper thumbnail of Lambert, N. & Freedman, R.B.Structural properties of homogeneous protein disulphide-isomerase from bovine liver purified by a rapid high-yielding procedure. Biochem. J. 213, 225−234

Biochemical Journal

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Research paper thumbnail of Poor diet and smoking : the big killers : comparing health education in two hazard domains

British Food Journal

Encouraging the UK public to quit smoking has been a public health feature for over a century to ... more Encouraging the UK public to quit smoking has been a public health feature for over a century to a greater or lesser degree. Persuading people to consume five or more portions of fruits and vegetables is a far newer health policy, with a history of only some ten years. The article compares the established anti-smoking campaign with that of the

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Research paper thumbnail of The latency of rat liver microsomal protein disulfide-isomerase

Biochemical Journal

ABSTRACT

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Research paper thumbnail of Protocol for purification of pea storage proteins and characterization of their aggregation state

Biochemical Society Transactions, 1986

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Research paper thumbnail of The dissociation of pea 11S storage protein by salt, as measured by light scattering

Biochemical Society Transactions, 1987

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Research paper thumbnail of Effect of ethoxyquin on glutathione levels and peroxidisability of rat liver membranes

Biochemical Society transactions, 1996

... of rat liver membranes. GEOFFREY W. PLUMB, NIGEL LAMBERT, STEPHEN J.CHAMBERS, HELEN L. CLARKE... more ... of rat liver membranes. GEOFFREY W. PLUMB, NIGEL LAMBERT, STEPHEN J.CHAMBERS, HELEN L. CLARKE, MARGARET M. MANSON, GORDON NEAL and GARY WILLIAMSON. Department of Food Molecular Biochemistry ...

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Research paper thumbnail of Influence of phenolics on protein binding to the antioxidant responsive element (ARE)

Biochemical Society transactions, 1996

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Research paper thumbnail of Purification of a cytosolic enzyme from human liver with phospholipid hydroperoxide glutathione peroxidase activity

The International journal of biochemistry

Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is a selenoprotein which inhibits perox... more Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is a selenoprotein which inhibits peroxidation of microsomes. The human enzyme, which may play an important role in protecting the cell from oxidative damage, has not been purified or characterized. PHGPx was isolated from human liver using ammonium sulphate fractionation, affinity chromatography on bromosulphophthalein-glutathione-agarose, gel filtration on Sephadex G-50, anion exchange chromatography on Mono Q resin and high resolution gel filtration on Superdex 75. The protein was purified about 112,000-fold, and 12 micrograms was obtained from 140 g of human liver with a 9% yield. PHGPx was active on hydrogen peroxide, cumene hydroperoxide, linoleic acid hydroperoxide and phosphatidylcholine hydroperoxide. The molecular weight, as estimated from non-denaturing gel filtration, was 16,100. The turnover number (37 degrees C, pH 7.6) on (beta-(13-hydroperoxy-cis-9, trans-11-octadecadienoyl)-gamma-palmitoyl)-L-alpha-phosphatid...

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Research paper thumbnail of Evaluation of the antioxidant properties of food extracts

Biochemical Society transactions, 1995

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Research paper thumbnail of Identification of protein disulphide-isomerase as a major acidic polypeptide in rat liver microsomal membranes

The Biochemical journal, 1983

Protein disulphide-isomerase was purified to homogeneity from rat liver by a rapid high-yielding ... more Protein disulphide-isomerase was purified to homogeneity from rat liver by a rapid high-yielding procedure. Structural properties of the pure enzyme were very similar to those of the bovine liver enzyme purified by the same method. The purified rat liver enzyme was subjected to two-dimensional gel electrophoresis in the presence and in the absence of microsomal membranes, and shown to co-electrophorese with a major acidic polypeptide clearly identifiable in the two-dimensional electrophoretic profile of microsomal membranes. This identification was confirmed by peptide 'mapping' of the pure enzyme and of the defined spot from a two-dimensional electrophoresis gel.

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Research paper thumbnail of Kinetics and specificity of homogeneous protein disulphide-isomerase in protein disulphide isomerization and in thiol-protein-disulphide oxidoreduction

The Biochemical journal, 1983

The protein disulphide-bond isomerization activity of highly active homogeneous protein disulphid... more The protein disulphide-bond isomerization activity of highly active homogeneous protein disulphide-isomerase (measured by re-activation of 'scrambled' ribonuclease) is enhanced by EDTA and by phosphate buffers. As shown for previous less-active preparations, the enzyme has a narrow pH optimum around pH 7.8 and requires the presence of either a dithiol or a thiol. The dithiol dithiothreitol is effective at concentrations 100-fold lower than the monothiols reduced glutathione and cysteamine. The enzyme follows Michaelis-Menten kinetics with respect to these substrates; Km values are 4,620 and 380 microM respectively. The enzyme shows apparent inhibition by high concentrations of thiol or dithiol compounds (greater than 10 X Km), but the effect is mainly on the extent of reaction, not the initial rate. This is interpreted as indicating the formation of significant amounts of reduced ribonuclease in these more reducing conditions. The purified enzyme will also catalyse net reduc...

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Research paper thumbnail of Norleg

Nutrition & Food Science, 1991

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Research paper thumbnail of New Looks at Neglected Legumes

Nutrition & Food Science, 1989

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Modified procedure for rapid purification of protein disulphide-isomerase from bovine liver

Biochemical Society Transactions, 1982

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Chemical modification of essential residues in purified rat liver protein disulphide-isomerase

Biochemical Society Transactions, 1984

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Assessing patients' preferences for treatments for angina using a modified repertory grid method

Soc Sci Med 60 2585 2595, Jun 1, 2005

A current popular theme in medicine concerns whether and how patients should be involved in treat... more A current popular theme in medicine concerns whether and how patients should be involved in treatment choice. Assuming patient involvement is desirable, how should one go about eliciting preferences? A variety of quantitative and qualitative methods exist that may be used for this purpose, one of which is the repertory grid method. This method involves eliciting constructs (reasons) for preferences through comparing sets of three options. This method allows the structured elicitation of the reasons behind individual preferences, but also, when used with generalised procrustes analysis (GPA), allows aggregation of individual data to reveal general preference patterns. In this study the repertory grid method was used to examine patient preferences for angina treatments with the goal of, first, gaining some understanding of general patterns of patient preference, and second, examining the likely utility of the technique in this setting. A sample of 21 patients with mild and stable angina from two general practices in Norfolk, UK was interviewed using the repertory grid method to elicit the constructs underlying their preferences amongst seven angina treatments (including 'no treatment'). Individualised questionnaires were then produced and sent to the patients for self-completion, which required rating the extent to which each construct was relevant for each treatment (scored on visual analogue rating scales). Analysis of the ratings, using GPA, showed that the constructs clustered around two dimensions: 'some treatment' versus 'no treatment', and drug treatment versus surgical treatment. While some treatment was generally preferred to no treatment, individuals varied in preference for drug treatments or surgical treatments. Although the latter were generally perceived as 'effective' they were also perceived, for example, as 'invasive', 'frightening', related to 'negative experiences', and being more appropriate for when symptoms are severe ('proportionate'). We consider the implications of these results for involving patients in choosing amongst treatments.

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Research paper thumbnail of Understanding patients' preferences for treatment: the need for innovative methodologies

Bookmarks Related papers MentionsView impact

Research paper thumbnail of A study of patient expectations in a Norfolk general practice: Patient expectations in a Norfolk general practice

Health Expectations an International Journal of Public Participation in Health Care and Health Policy, Sep 1, 2010

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Research paper thumbnail of Characterisation and crystallisation of an 11S seed storage globulin from coconut (Cocos nucifera)

Food Chemistry, 1990

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Research paper thumbnail of Smoking cessation and vegetable consumption : reducing cancer through communication?

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Research paper thumbnail of Lambert, N. & Freedman, R.B.Structural properties of homogeneous protein disulphide-isomerase from bovine liver purified by a rapid high-yielding procedure. Biochem. J. 213, 225−234

Biochemical Journal

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Research paper thumbnail of Poor diet and smoking : the big killers : comparing health education in two hazard domains

British Food Journal

Encouraging the UK public to quit smoking has been a public health feature for over a century to ... more Encouraging the UK public to quit smoking has been a public health feature for over a century to a greater or lesser degree. Persuading people to consume five or more portions of fruits and vegetables is a far newer health policy, with a history of only some ten years. The article compares the established anti-smoking campaign with that of the

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Research paper thumbnail of The latency of rat liver microsomal protein disulfide-isomerase

Biochemical Journal

ABSTRACT

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Research paper thumbnail of Protocol for purification of pea storage proteins and characterization of their aggregation state

Biochemical Society Transactions, 1986

Bookmarks Related papers MentionsView impact

Research paper thumbnail of The dissociation of pea 11S storage protein by salt, as measured by light scattering

Biochemical Society Transactions, 1987

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Effect of ethoxyquin on glutathione levels and peroxidisability of rat liver membranes

Biochemical Society transactions, 1996

... of rat liver membranes. GEOFFREY W. PLUMB, NIGEL LAMBERT, STEPHEN J.CHAMBERS, HELEN L. CLARKE... more ... of rat liver membranes. GEOFFREY W. PLUMB, NIGEL LAMBERT, STEPHEN J.CHAMBERS, HELEN L. CLARKE, MARGARET M. MANSON, GORDON NEAL and GARY WILLIAMSON. Department of Food Molecular Biochemistry ...

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Research paper thumbnail of Influence of phenolics on protein binding to the antioxidant responsive element (ARE)

Biochemical Society transactions, 1996

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Research paper thumbnail of Purification of a cytosolic enzyme from human liver with phospholipid hydroperoxide glutathione peroxidase activity

The International journal of biochemistry

Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is a selenoprotein which inhibits perox... more Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is a selenoprotein which inhibits peroxidation of microsomes. The human enzyme, which may play an important role in protecting the cell from oxidative damage, has not been purified or characterized. PHGPx was isolated from human liver using ammonium sulphate fractionation, affinity chromatography on bromosulphophthalein-glutathione-agarose, gel filtration on Sephadex G-50, anion exchange chromatography on Mono Q resin and high resolution gel filtration on Superdex 75. The protein was purified about 112,000-fold, and 12 micrograms was obtained from 140 g of human liver with a 9% yield. PHGPx was active on hydrogen peroxide, cumene hydroperoxide, linoleic acid hydroperoxide and phosphatidylcholine hydroperoxide. The molecular weight, as estimated from non-denaturing gel filtration, was 16,100. The turnover number (37 degrees C, pH 7.6) on (beta-(13-hydroperoxy-cis-9, trans-11-octadecadienoyl)-gamma-palmitoyl)-L-alpha-phosphatid...

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Research paper thumbnail of Evaluation of the antioxidant properties of food extracts

Biochemical Society transactions, 1995

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Identification of protein disulphide-isomerase as a major acidic polypeptide in rat liver microsomal membranes

The Biochemical journal, 1983

Protein disulphide-isomerase was purified to homogeneity from rat liver by a rapid high-yielding ... more Protein disulphide-isomerase was purified to homogeneity from rat liver by a rapid high-yielding procedure. Structural properties of the pure enzyme were very similar to those of the bovine liver enzyme purified by the same method. The purified rat liver enzyme was subjected to two-dimensional gel electrophoresis in the presence and in the absence of microsomal membranes, and shown to co-electrophorese with a major acidic polypeptide clearly identifiable in the two-dimensional electrophoretic profile of microsomal membranes. This identification was confirmed by peptide 'mapping' of the pure enzyme and of the defined spot from a two-dimensional electrophoresis gel.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Kinetics and specificity of homogeneous protein disulphide-isomerase in protein disulphide isomerization and in thiol-protein-disulphide oxidoreduction

The Biochemical journal, 1983

The protein disulphide-bond isomerization activity of highly active homogeneous protein disulphid... more The protein disulphide-bond isomerization activity of highly active homogeneous protein disulphide-isomerase (measured by re-activation of 'scrambled' ribonuclease) is enhanced by EDTA and by phosphate buffers. As shown for previous less-active preparations, the enzyme has a narrow pH optimum around pH 7.8 and requires the presence of either a dithiol or a thiol. The dithiol dithiothreitol is effective at concentrations 100-fold lower than the monothiols reduced glutathione and cysteamine. The enzyme follows Michaelis-Menten kinetics with respect to these substrates; Km values are 4,620 and 380 microM respectively. The enzyme shows apparent inhibition by high concentrations of thiol or dithiol compounds (greater than 10 X Km), but the effect is mainly on the extent of reaction, not the initial rate. This is interpreted as indicating the formation of significant amounts of reduced ribonuclease in these more reducing conditions. The purified enzyme will also catalyse net reduc...

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