Muhammad Imran Tousif | University of Education Lahore (original) (raw)

Papers by Muhammad Imran Tousif

Industrial Crops and Products, May 1, 2019

Abstract Plant from Caragana genus have interesting potential in folklore medicines and have been... more Abstract Plant from Caragana genus have interesting potential in folklore medicines and have been explored for various pharmacological activities. The aim of this study was to probe into the chemical and biological effects of different extracts of Caragana ambigua Stocks (Fabaceae). Total phenolic and total flavonoids contents were determined using standard spectrophotometric methods, whereas, the secondary metabolites composition was established by ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) analysis. Antioxidant potential was estimated via 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging, ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), phosphomolybdenum and metal chelating assays and the enzyme (cholinesterases, α-amylase, α-glucosidase and tyrosinase) inhibition potential were also assessed. Moreover, in silico docking studies were performed to highlight possible interactions between three major secondary metabolites and the tested enzymes. The ethyl acetate extract exhibited higher phenolic (85.87 ± 2.96 mg GAE/g) and flavonoid (66.45 ± 0.37 mg RE/g) contents, which, we propose, are responsible for its higher radical scavenging, reducing power, total antioxidant capacity and tyrosinase inhibition. The n-hexane extract showed stronger anti-cholinesterase and anti-diabetic property. Similarly, UHPLC-MS profiling of methanol and ethyl acetate extracts identified the presence of flavonoids, phenolics, alkaloids, and coumarin derivatives. Three of the dominant compounds (isobergaptene, jujubasaponin IV and phellodensin D), were docked against all enzymes and their affinity were compared. Among these compounds, phellodensin D showed highest affinity towards all the studied enzymes. It is therefore, concluded that extracts from C. ambigua showed potent antioxidant and enzyme inhibition potential with potent bioactive molecules which could open new industrial applications.

Journal of Molecular Structure, Nov 1, 2023

Chemistry of Natural Compounds, 2019

A new aromatic polyketide compound, arabomycin (1), along with three known metabolites (2-4), was... more A new aromatic polyketide compound, arabomycin (1), along with three known metabolites (2-4), was isolated from the culture broth of the marine bacterium Streptomyces sp. PGC 32. The structure of the new compound was established through 1D and 2D NMR analyses and high-resolution mass spectrometry, and the known compounds were elucidated on the basis of 1 H and 13 C NMR information in comparison with literature values. Arabomycin (1) was found to have potential antibacterial action against the Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus, and Gram-negative bacteria Escherichia coli and Enterobacter sp.

Industrial Crops and Products, Dec 1, 2019

The plants of genus Neurada have been utilized for medicinal purposes. This study probed into the... more The plants of genus Neurada have been utilized for medicinal purposes. This study probed into the biological propensities and endeavored to achieve the detailed phytochemical profile, via ultra-high performance liquid chromatography-UHPLC-MS analysis and high performance liquid chromatography photodiode array-HPLC-PDA of different polarity extracts (methanol, n-hexane, chloroform, and n-butanol) of Neurada procumbens L. The biological features were studied by determining the antioxidant potential via 2,2-Diphenyl-1-picrylhydrazyl-DPPH, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid-ABTS, ferric reducing antioxidant power-FRAP, cupric reducing antioxidant power-CUPRAC, phoshomolybdenum and metal chelation assays and clinically significant major enzymes (cholinesterases, α-amylase, α-glucosidase and tyrosinase inhibition). Moreover, the correlation among the biological activities and total bioactive contents of the extracts were studied via multivariate statistical analysis. The methanol and n-butanol extracts revealed the presence of total phenolic and flavonoid contents in highest concentrations which tend to correlate with their maximum anti-oxidant capacities for radical scavenging and reducing power assays. The n-hexane extract was most active in the phosphomolybdenum assays whereas the chloroform extracts showed the highest metal chelation potential. The UHPLC-MS phytochemical profiling of the methanolic extract (both positive and negative ionization mode) revealed the existence of eighteen phytochemicals representing four different classes (phenolics, flavonoids, sesquiterpenoids, and alkaloids). The n-butanol extract inhibited acetylcholinesterase (4.10 mg galantamine equivalent-GALAE/g) and tyrosinase (127.65 mg kojic acid equivalent-KAE/g) significantly as compared to the control. The n-hexane extract exhibited highest inhibition against butyrylcholinesterase (1.93 mg GALAE/g) and α-glucosidase (59.0 mmol acarbose equivalent-ACAE/g), while chloroform extract exhibited prominent inhibitory action inimical to α-amylase (0.65 mmol ACAE/g). Overall, it was observed that FRAP and tyrosinase activities were the most contributive biological activities for the formation of first component with p values of 0.018 and 0.023, respectively, whereas, for the formation of second component, glucosidase activity was the most contributive assay with p value of 0.002. It can be concluded that the manifestation of dynamic phytochemicals with multipharmacological potential marks N. procumbens as a prospective origin of health-promoting molecules warrants further exploration as a unique therapeutic medicinal plant.

Phytochemistry Letters, Sep 1, 2017

The chromatographic purification of the n-hexane soluble part of methanolic extract of Seriphidiu... more The chromatographic purification of the n-hexane soluble part of methanolic extract of Seriphidium oliverianum (J. Gay ex Besser) yielded six secondary metabolites that includes two new natural products; diethyl-2,6-diphenyl-4-(3-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate (1) and 2,6-diacetyl-3,5-dimethyl-4-(3-nitrophenyl)-1,4-dihydropyridine (2) along with four known phytochemicals; tetradecyl ferulate (3), octadecyl coumarate (4), methyl coumarate (5) and methyl grevillate (6). The new compounds were characterized with the help of 1D-, 2D-NMR and high resolution mass spectrometric techniques, whereas, the known compounds were identified through 1D-NMR and mass spectrometry, and in comparison with the literature values. Compound 1 is synthetically known, however, it is first time reported from any natural source, whereas, compound 2 has never been reported from any other source before. All the isolates were screened in anti-urease and anti-glucosidase bioassay but were found inactive.

Arabian Journal of Chemistry, May 1, 2023

Frontiers in Chemistry, Nov 11, 2022

fraction was the most active against Serratia marcescens, Stenotrophomonas maltophilia, Bacillus ... more fraction was the most active against Serratia marcescens, Stenotrophomonas maltophilia, Bacillus subtilis, Klebsiella pneumonia, and Staphylococcus aureus, while the rest of the fractions showed good to moderate activity. Enzyme inhibition assays showed that ROEA fraction exhibited the highest activity with IC 50 values of 2.78 ± 0.42 and 3.95 ± 0.13 mg/mL against urease and carbonic anhydrase (CA), respectively. Furthermore, the docking studies of some of the major compounds identified in the extract revealed a strong correlation with their inhibitory activity. All extracts and fractions were also tested for their thrombolytic activity, and the ROB fraction showed a notable potential. Antiviral assay led to remarkable outcomes. Thus, it can be inferred that aerial parts of R. odorata are potential sources of bioactive components with several significant pharmacological activities. KEYWORDS Rondeletia odorata, phytochemical profiling, in vitro biological activity, docking studies, cytotoxicity assessments Frontiers in Chemistry frontiersin.org 02 Khursheed et al.

South African Journal of Botany, May 1, 2021

Abstract Alnus nitida (Spach) Endl has been used as a remedy for inflammation-related disorders i... more Abstract Alnus nitida (Spach) Endl has been used as a remedy for inflammation-related disorders in the folk medicinal system by the inhabitants of the northern areas of Pakistan. This study deals with the phytochemical composition, cytotoxicity, and anti-inflammatory activity of various extracts of A. nitida. The phytochemical composition was established by measuring total phenolic contents through spectrophotometrically and chemical profiling was done by GC–MS analysis. Cytotoxicity was tested against four cancer cell lines (SK-MEL-28, KB, BT-549, and SK-OV-3) and two noncancerous kidney cell lines (LLC-PK1 and Vero). The hexanes extract showed promising activity against SK-MEL-28, BT-549, SK-OV-3 and LLC-PK1 cell lines with IC50 values ranging from 13–78 μg/mL and hydro-methanol extract inhibited the growth of KB, BT-549, and SK-OV-3 with IC50 values ranging from 25–42 μg/mL. The hydro-methanol, hexanes, and chloroform extracts showed anti-inflammatory activity by inhibiting iNOS in LPS-induced macrophages with IC50 values of 69, 60, and 88 µg/mL, respectively. Besides, palmitic acid and parthenolide were theoretically docked against iNOS synthase. Parthenolide showed higher inhibition affinity (- 6.16 kcal/mol, Ki = 30.63 µM) in comparison to the control ligand (- 5.28 kcal/mol, Ki = 135.78 µM). This study showed that the A. nitida extracts possess anti-inflammatory and cytotoxic activities, indicating A. nitida as a potential candidate for the discovery of novel phytopharmaceutical products and industrial applications.

Molecules, Oct 18, 2022

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Chemistry & Biodiversity, Oct 17, 2022

Chirality, Nov 19, 2013

The electronic circular dichroism (ECD) spectra of two sesquiterpenoids (1 and 2) related to oplo... more The electronic circular dichroism (ECD) spectra of two sesquiterpenoids (1 and 2) related to oplopanone, obtained from a methanolic extract of the plant Serphidium stenocephalum (Artemisia stenocephala), were measured and reproduced by means of timedependent density functional theory (TDDFT) calculations, establishing their absolute configuration. The application of ketone octant rule for carbonyl n-π* ECD band to compounds 1 and 2, which include an acyclic carbonyl group, was critically assessed. The peculiar oplopanone skeleton makes a straightforward application of the octant rule impossible, because of the uncertainty related to the shape of the so-called third nodal surface separating front and back octants. The various group contributions to the carbonyl n-π* ECD band were estimated with TDDFT calculations on selected molecular models obtained by consecutive dissections from 1.

Anti-inflammatory & anti-allergy agents in medicinal chemistry, Dec 10, 2018

Serine protease, Human Neutrophil Elastase (HNE), has been shown to be useful in medical science,... more Serine protease, Human Neutrophil Elastase (HNE), has been shown to be useful in medical science, however, its over production and malfunctioning may produce devastating effects and cause serious damage to the host. Unfortunately, the present approved drug, sivelestat, only alleviates the symptoms of the diseases caused by malfunction of HNE but not the disease progression. Therefore, there is a crucial need to search potent and safer molecules as elastase inhibitors and to develop better anti-inflammatory drugs in future. In addition, nature is the best architect that may provide a safer future drug candidate as HNEproduction/activity inhibitor. Since phenolic natural products are already known as antiinflammatory compounds, either by acting as antioxidants or by any other mechanism, thus, this review article summarizes the discovery and elastase inhibitory activity of ∼180 phenolics isolated from diverse natural sources during more than one decade, i.e. 2005-2017.

Food bioscience, Apr 1, 2023

Frontiers in chemical sciences, Jun 30, 2020

Two steroids glycosides solamargine (1) and dioscine (2) were isolated from methanolic extract of... more Two steroids glycosides solamargine (1) and dioscine (2) were isolated from methanolic extract of Solanum surattense and evaluated for their anticancer activity. The structure of the isolated compounds was identified through 1D and 2D NMR techniques, mass spectrometry and in comparison, with the literature values of their spectroscopic data. Both compounds showed the excellent anticancer activity against NIH-3T3 fibroblast cancer cell line with IC50 value of 7.55±1.5 and 3.3±1.9µg/ml respectively which were comparable with control Cyclohexamide which showed the IC50 value of 0.8± 0.2 µg/ml.

Chemistry & Biodiversity, Mar 29, 2022

Leptadenia pyrotechnica(Forssk.) Decne is growing in Cholistan desert, and is known for its laxat... more Leptadenia pyrotechnica(Forssk.) Decne is growing in Cholistan desert, and is known for its laxative, analgesic, anabolic and astringent, antioxidant, anti‐inflammatory, antibacterial, antitumor, hypolipidemic and antiatherosclerotic properties. The present study disclosed the metabolic picture ofL. pyrotechnicaand validates its folk uses. LP‐H fraction constitute 25.79±0.11 mgGAE/g extract and 20.64±0.33 mgRE/g extract of phenolic and flavonoid content, respectively, followed by LP‐E (23.15±0.33 mgGAE/g extract and 19.40±0.13 mgRE/g extract), however, LP‐E exhibited the highest free radical scavenging (DPPH: 21.05±0.45mgTE/g and ABTS: 68.12±0.53 mgTE/g) and metal reducing (FRAP: 44.93±1.66, CUPRAC: 117.42±1.28 mgTE/g, respectively) activities. The total antioxidant capacity in Phosphomolybdenum assay (1.52±0.14 mmolTE/g) and ferrous ion chelating (11.57±0.29 mgEDTAE/g) activities were observed highest for LP‐H extract. In cholinesterase's inhibitory assays, the LP‐E and LP‐W extracts exhibited inhibitory values as 2.43 and 2.40±0.07 mgGALAE/g extract, respectively against AChE, while against BChE the LP‐H displayed the highest value as 5.98±0.44 mgGALAE/g extract. The LP‐H fraction also showed the highest inhibition potential (7.72±0.14 mmol ACAE/g and 0.55±0.01 mmol ACAE/g, respectively) against α‐glucosidase and α‐amylase enzymes, while, in tyrosinase inhibitory assay, all the fractions exhibited significant activities in the range of 59.35±0.29 to 55.18±0.49 mgKAE/g extract. RP‐UHPLC/MS analysis of LP‐M disclosed the presence of 57 metabolites of various classes. A multivariate analysis and molecular docking study was also carried out to establish relationships between the metabolites and the biological activities, which finally validate the use ofL. pyrotechnicaas herbal medicine or component nutraceutical, food and cosmetic industry.

Journal of Molecular Structure

Critical Reviews in Food Science and Nutrition, May 31, 2023

Critical Reviews in Food Science and Nutrition

Industrial Crops and Products, May 1, 2019

Abstract Plant from Caragana genus have interesting potential in folklore medicines and have been... more Abstract Plant from Caragana genus have interesting potential in folklore medicines and have been explored for various pharmacological activities. The aim of this study was to probe into the chemical and biological effects of different extracts of Caragana ambigua Stocks (Fabaceae). Total phenolic and total flavonoids contents were determined using standard spectrophotometric methods, whereas, the secondary metabolites composition was established by ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) analysis. Antioxidant potential was estimated via 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging, ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), phosphomolybdenum and metal chelating assays and the enzyme (cholinesterases, α-amylase, α-glucosidase and tyrosinase) inhibition potential were also assessed. Moreover, in silico docking studies were performed to highlight possible interactions between three major secondary metabolites and the tested enzymes. The ethyl acetate extract exhibited higher phenolic (85.87 ± 2.96 mg GAE/g) and flavonoid (66.45 ± 0.37 mg RE/g) contents, which, we propose, are responsible for its higher radical scavenging, reducing power, total antioxidant capacity and tyrosinase inhibition. The n-hexane extract showed stronger anti-cholinesterase and anti-diabetic property. Similarly, UHPLC-MS profiling of methanol and ethyl acetate extracts identified the presence of flavonoids, phenolics, alkaloids, and coumarin derivatives. Three of the dominant compounds (isobergaptene, jujubasaponin IV and phellodensin D), were docked against all enzymes and their affinity were compared. Among these compounds, phellodensin D showed highest affinity towards all the studied enzymes. It is therefore, concluded that extracts from C. ambigua showed potent antioxidant and enzyme inhibition potential with potent bioactive molecules which could open new industrial applications.

Journal of Molecular Structure, Nov 1, 2023

Chemistry of Natural Compounds, 2019

A new aromatic polyketide compound, arabomycin (1), along with three known metabolites (2-4), was... more A new aromatic polyketide compound, arabomycin (1), along with three known metabolites (2-4), was isolated from the culture broth of the marine bacterium Streptomyces sp. PGC 32. The structure of the new compound was established through 1D and 2D NMR analyses and high-resolution mass spectrometry, and the known compounds were elucidated on the basis of 1 H and 13 C NMR information in comparison with literature values. Arabomycin (1) was found to have potential antibacterial action against the Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus, and Gram-negative bacteria Escherichia coli and Enterobacter sp.

Industrial Crops and Products, Dec 1, 2019

The plants of genus Neurada have been utilized for medicinal purposes. This study probed into the... more The plants of genus Neurada have been utilized for medicinal purposes. This study probed into the biological propensities and endeavored to achieve the detailed phytochemical profile, via ultra-high performance liquid chromatography-UHPLC-MS analysis and high performance liquid chromatography photodiode array-HPLC-PDA of different polarity extracts (methanol, n-hexane, chloroform, and n-butanol) of Neurada procumbens L. The biological features were studied by determining the antioxidant potential via 2,2-Diphenyl-1-picrylhydrazyl-DPPH, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid-ABTS, ferric reducing antioxidant power-FRAP, cupric reducing antioxidant power-CUPRAC, phoshomolybdenum and metal chelation assays and clinically significant major enzymes (cholinesterases, α-amylase, α-glucosidase and tyrosinase inhibition). Moreover, the correlation among the biological activities and total bioactive contents of the extracts were studied via multivariate statistical analysis. The methanol and n-butanol extracts revealed the presence of total phenolic and flavonoid contents in highest concentrations which tend to correlate with their maximum anti-oxidant capacities for radical scavenging and reducing power assays. The n-hexane extract was most active in the phosphomolybdenum assays whereas the chloroform extracts showed the highest metal chelation potential. The UHPLC-MS phytochemical profiling of the methanolic extract (both positive and negative ionization mode) revealed the existence of eighteen phytochemicals representing four different classes (phenolics, flavonoids, sesquiterpenoids, and alkaloids). The n-butanol extract inhibited acetylcholinesterase (4.10 mg galantamine equivalent-GALAE/g) and tyrosinase (127.65 mg kojic acid equivalent-KAE/g) significantly as compared to the control. The n-hexane extract exhibited highest inhibition against butyrylcholinesterase (1.93 mg GALAE/g) and α-glucosidase (59.0 mmol acarbose equivalent-ACAE/g), while chloroform extract exhibited prominent inhibitory action inimical to α-amylase (0.65 mmol ACAE/g). Overall, it was observed that FRAP and tyrosinase activities were the most contributive biological activities for the formation of first component with p values of 0.018 and 0.023, respectively, whereas, for the formation of second component, glucosidase activity was the most contributive assay with p value of 0.002. It can be concluded that the manifestation of dynamic phytochemicals with multipharmacological potential marks N. procumbens as a prospective origin of health-promoting molecules warrants further exploration as a unique therapeutic medicinal plant.

Phytochemistry Letters, Sep 1, 2017

The chromatographic purification of the n-hexane soluble part of methanolic extract of Seriphidiu... more The chromatographic purification of the n-hexane soluble part of methanolic extract of Seriphidium oliverianum (J. Gay ex Besser) yielded six secondary metabolites that includes two new natural products; diethyl-2,6-diphenyl-4-(3-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate (1) and 2,6-diacetyl-3,5-dimethyl-4-(3-nitrophenyl)-1,4-dihydropyridine (2) along with four known phytochemicals; tetradecyl ferulate (3), octadecyl coumarate (4), methyl coumarate (5) and methyl grevillate (6). The new compounds were characterized with the help of 1D-, 2D-NMR and high resolution mass spectrometric techniques, whereas, the known compounds were identified through 1D-NMR and mass spectrometry, and in comparison with the literature values. Compound 1 is synthetically known, however, it is first time reported from any natural source, whereas, compound 2 has never been reported from any other source before. All the isolates were screened in anti-urease and anti-glucosidase bioassay but were found inactive.

Arabian Journal of Chemistry, May 1, 2023

Frontiers in Chemistry, Nov 11, 2022

fraction was the most active against Serratia marcescens, Stenotrophomonas maltophilia, Bacillus ... more fraction was the most active against Serratia marcescens, Stenotrophomonas maltophilia, Bacillus subtilis, Klebsiella pneumonia, and Staphylococcus aureus, while the rest of the fractions showed good to moderate activity. Enzyme inhibition assays showed that ROEA fraction exhibited the highest activity with IC 50 values of 2.78 ± 0.42 and 3.95 ± 0.13 mg/mL against urease and carbonic anhydrase (CA), respectively. Furthermore, the docking studies of some of the major compounds identified in the extract revealed a strong correlation with their inhibitory activity. All extracts and fractions were also tested for their thrombolytic activity, and the ROB fraction showed a notable potential. Antiviral assay led to remarkable outcomes. Thus, it can be inferred that aerial parts of R. odorata are potential sources of bioactive components with several significant pharmacological activities. KEYWORDS Rondeletia odorata, phytochemical profiling, in vitro biological activity, docking studies, cytotoxicity assessments Frontiers in Chemistry frontiersin.org 02 Khursheed et al.

South African Journal of Botany, May 1, 2021

Abstract Alnus nitida (Spach) Endl has been used as a remedy for inflammation-related disorders i... more Abstract Alnus nitida (Spach) Endl has been used as a remedy for inflammation-related disorders in the folk medicinal system by the inhabitants of the northern areas of Pakistan. This study deals with the phytochemical composition, cytotoxicity, and anti-inflammatory activity of various extracts of A. nitida. The phytochemical composition was established by measuring total phenolic contents through spectrophotometrically and chemical profiling was done by GC–MS analysis. Cytotoxicity was tested against four cancer cell lines (SK-MEL-28, KB, BT-549, and SK-OV-3) and two noncancerous kidney cell lines (LLC-PK1 and Vero). The hexanes extract showed promising activity against SK-MEL-28, BT-549, SK-OV-3 and LLC-PK1 cell lines with IC50 values ranging from 13–78 μg/mL and hydro-methanol extract inhibited the growth of KB, BT-549, and SK-OV-3 with IC50 values ranging from 25–42 μg/mL. The hydro-methanol, hexanes, and chloroform extracts showed anti-inflammatory activity by inhibiting iNOS in LPS-induced macrophages with IC50 values of 69, 60, and 88 µg/mL, respectively. Besides, palmitic acid and parthenolide were theoretically docked against iNOS synthase. Parthenolide showed higher inhibition affinity (- 6.16 kcal/mol, Ki = 30.63 µM) in comparison to the control ligand (- 5.28 kcal/mol, Ki = 135.78 µM). This study showed that the A. nitida extracts possess anti-inflammatory and cytotoxic activities, indicating A. nitida as a potential candidate for the discovery of novel phytopharmaceutical products and industrial applications.

Molecules, Oct 18, 2022

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Chemistry & Biodiversity, Oct 17, 2022

Chirality, Nov 19, 2013

The electronic circular dichroism (ECD) spectra of two sesquiterpenoids (1 and 2) related to oplo... more The electronic circular dichroism (ECD) spectra of two sesquiterpenoids (1 and 2) related to oplopanone, obtained from a methanolic extract of the plant Serphidium stenocephalum (Artemisia stenocephala), were measured and reproduced by means of timedependent density functional theory (TDDFT) calculations, establishing their absolute configuration. The application of ketone octant rule for carbonyl n-π* ECD band to compounds 1 and 2, which include an acyclic carbonyl group, was critically assessed. The peculiar oplopanone skeleton makes a straightforward application of the octant rule impossible, because of the uncertainty related to the shape of the so-called third nodal surface separating front and back octants. The various group contributions to the carbonyl n-π* ECD band were estimated with TDDFT calculations on selected molecular models obtained by consecutive dissections from 1.

Anti-inflammatory & anti-allergy agents in medicinal chemistry, Dec 10, 2018

Serine protease, Human Neutrophil Elastase (HNE), has been shown to be useful in medical science,... more Serine protease, Human Neutrophil Elastase (HNE), has been shown to be useful in medical science, however, its over production and malfunctioning may produce devastating effects and cause serious damage to the host. Unfortunately, the present approved drug, sivelestat, only alleviates the symptoms of the diseases caused by malfunction of HNE but not the disease progression. Therefore, there is a crucial need to search potent and safer molecules as elastase inhibitors and to develop better anti-inflammatory drugs in future. In addition, nature is the best architect that may provide a safer future drug candidate as HNEproduction/activity inhibitor. Since phenolic natural products are already known as antiinflammatory compounds, either by acting as antioxidants or by any other mechanism, thus, this review article summarizes the discovery and elastase inhibitory activity of ∼180 phenolics isolated from diverse natural sources during more than one decade, i.e. 2005-2017.

Food bioscience, Apr 1, 2023

Frontiers in chemical sciences, Jun 30, 2020

Two steroids glycosides solamargine (1) and dioscine (2) were isolated from methanolic extract of... more Two steroids glycosides solamargine (1) and dioscine (2) were isolated from methanolic extract of Solanum surattense and evaluated for their anticancer activity. The structure of the isolated compounds was identified through 1D and 2D NMR techniques, mass spectrometry and in comparison, with the literature values of their spectroscopic data. Both compounds showed the excellent anticancer activity against NIH-3T3 fibroblast cancer cell line with IC50 value of 7.55±1.5 and 3.3±1.9µg/ml respectively which were comparable with control Cyclohexamide which showed the IC50 value of 0.8± 0.2 µg/ml.

Chemistry & Biodiversity, Mar 29, 2022

Leptadenia pyrotechnica(Forssk.) Decne is growing in Cholistan desert, and is known for its laxat... more Leptadenia pyrotechnica(Forssk.) Decne is growing in Cholistan desert, and is known for its laxative, analgesic, anabolic and astringent, antioxidant, anti‐inflammatory, antibacterial, antitumor, hypolipidemic and antiatherosclerotic properties. The present study disclosed the metabolic picture ofL. pyrotechnicaand validates its folk uses. LP‐H fraction constitute 25.79±0.11 mgGAE/g extract and 20.64±0.33 mgRE/g extract of phenolic and flavonoid content, respectively, followed by LP‐E (23.15±0.33 mgGAE/g extract and 19.40±0.13 mgRE/g extract), however, LP‐E exhibited the highest free radical scavenging (DPPH: 21.05±0.45mgTE/g and ABTS: 68.12±0.53 mgTE/g) and metal reducing (FRAP: 44.93±1.66, CUPRAC: 117.42±1.28 mgTE/g, respectively) activities. The total antioxidant capacity in Phosphomolybdenum assay (1.52±0.14 mmolTE/g) and ferrous ion chelating (11.57±0.29 mgEDTAE/g) activities were observed highest for LP‐H extract. In cholinesterase's inhibitory assays, the LP‐E and LP‐W extracts exhibited inhibitory values as 2.43 and 2.40±0.07 mgGALAE/g extract, respectively against AChE, while against BChE the LP‐H displayed the highest value as 5.98±0.44 mgGALAE/g extract. The LP‐H fraction also showed the highest inhibition potential (7.72±0.14 mmol ACAE/g and 0.55±0.01 mmol ACAE/g, respectively) against α‐glucosidase and α‐amylase enzymes, while, in tyrosinase inhibitory assay, all the fractions exhibited significant activities in the range of 59.35±0.29 to 55.18±0.49 mgKAE/g extract. RP‐UHPLC/MS analysis of LP‐M disclosed the presence of 57 metabolites of various classes. A multivariate analysis and molecular docking study was also carried out to establish relationships between the metabolites and the biological activities, which finally validate the use ofL. pyrotechnicaas herbal medicine or component nutraceutical, food and cosmetic industry.

Journal of Molecular Structure

Critical Reviews in Food Science and Nutrition, May 31, 2023

Critical Reviews in Food Science and Nutrition