Daniela Nicuta | Vasile Alecsandri University of Bacau, Romania (original) (raw)

Papers by Daniela Nicuta

Analele Stiintifice ale Universitatii "Alexandru Ioan Cuza" din Iasi Sec. II a. Genetica si Biologie Moleculara, Aug 10, 2007

Analele Stiintifice ale Universitatii "Alexandru Ioan Cuza" din Iasi Sec. II a. Genetica si Biologie Moleculara, Jan 3, 2007

Analele Stiintifice ale Universitatii "Alexandru Ioan Cuza" din Iasi Sec. II a. Genetica si Biologie Moleculara, Aug 15, 2003

Key words: Brasica juncea, in vitro, micropropagation. Abstract: This paper presents the morphoge... more Key words: Brasica juncea, in vitro, micropropagation. Abstract: This paper presents the morphogenetic reaction of different explants of Brassica juncea Czern. Species in in vitro cultures. The obtained results showed that the micropropagation of this plant could be recommended only for ...

DOAJ (DOAJ: Directory of Open Access Journals), Dec 1, 2011

The UV-A radiation acts on cellular constituents indirectly through oxidative mechanisms involvin... more The UV-A radiation acts on cellular constituents indirectly through oxidative mechanisms involving the radicals' formation. These species have a relatively short lifespan, but are highly reactive, reacts with most cellular constituents: nucleic acids, proteins, lipids, polysaccharides. The rapeseed samples provided by Agricultural Research Center Secuieni, Neamţ (Romania) were irradiated with a wide band source (UV-B, UV-A, visible) with different non-radiation doses, equivalent to several hours of sun exposure. The exposure to UV radiation determines changes in the metabolism, within the growth and development processes, having positive or negative influences on plant health and vitality. The purpose of this study is to analyze different rapeseed samples in order to identify the differences according to non-radiation effect. Regarding the growth and development of height stems it has been observed that roots length and stem height of rape samples are highest at irradiation time around 280 minutes.

Environmental Engineering and Management Journal, 2012

This study reports the effect of UV-VIS rays on the spectroscopic indexes and the growth paramete... more This study reports the effect of UV-VIS rays on the spectroscopic indexes and the growth parameters of canola cotyledon. Canola seeds were exposed to incoherent continuously radiation with wavelengths between 300 and 750 nm emitted by a high pressure lamp, for different exposure times. An untreated sample is used for comparison purposes. Seed germination and allometric scaling were related to irradiance treatments. The unsaturation and carbonyl spectroscopic indexes changes in the canola cotyledon stems from irradiated seeds were evaluated by Fourier Transform Infrared FTIR spectrophotometric methods. FTIR spectra suggest that the processes of irradiation do not affect the chemical fingerprints (ca. 900-1200 cm −1) and had no significant effect (p > 0.05) to the molecular spectrum in lipid related functional groups. On the other side, the results of non parametric Kruskal-Wallis Test confirm that all investigated samples are independent. The main significant effect was obtained at root level to the sample irradiated for 160 minutes (χ 2 = 11.358, df = 3, p =0.010). The growth of the organ parts was characterized by a negative allometric factor for control, 280 minutes and 460 minutes irradiated samples. However, for 160 minutes irradiation time, an isometric scaling (a =1.013) was obtained. The results show that 177 kJ/m2 ultraviolet dose was not strong enough to induce changes in lipid conformation. Significant changes in terms of growth parameters of canola cotyledon can be observed mainly caused by continuously emission lines at 365 nm, 436 nm and 620 nm. In the cases of irradiated seeds the proportion of germinating seeds decreased compared with the control sample. The largest decrease occurs at 160 minutes irradiation time. In this case a strong absorption of the orange radiation on 620 nm is probable. It may be assumed that the canola seeds are negatively photoblastic, in most cases. This study will prospectively justify the response investigation of molecular structure and growth parameters to various external stresses induced on the canola seeds differentiated by the age of plant and by selected wavelengths.

This paper presents the quantitative determination of pigments extract of Thymus sp. from in vitr... more This paper presents the quantitative determination of pigments extract of Thymus sp. from in vitro culture and spontaneous flora. For in vitro culture were used phytohormones (indoleacetic acid-IAA, indole-3-butyric acid-IBA and benzylaminopurine-BAP) in order to obtain a high yield in development of Thymus sp. plants. The source of spontaneous flora of Thymus sp. was Bacau (Magura), Romania. The biochemical investigations realised on Thymus sp. using Thin Layer Chromatography and the UV-Vis spectrophotometry showed that the production of assimilating pigments is influenced by growth and lighting conditions of plants.

Analele Universităţii "Ovidius" Constanţa, Jul 1, 2022

The grape pomace hydroalcoholic extracts obtained by two different extraction methods were tested... more The grape pomace hydroalcoholic extracts obtained by two different extraction methods were tested for biostimulatory potential activity for Origanum vulgare L. cultures. The total polyphenols contents of extracts were evaluated by Folin-Ciocalteu method. Characteristics such as: pH, salinity, conductivity and total dissolved solids were determined. FTIR and UV spectra of extracts were also recorded. The effect of grape pomace extracts on growth and development of oregano plant were studied by carrying out in vitro propagation of oregano on Murashige and Skoog basal medium supplemented with different concentrations of grape pomace extracts. Biometric measurements, growth rate and biomass accumulation have been narrowly monitored for all samples and compared to the control sample. The results have shown that the morphogenetic response depends both on the proportion in which basal medium was supplemented and on the extraction method used. A stimulation of growth and development at a low concentration of grape pomace extracts has been noted. The best results were observed in the samples with 1 and 5 % of grape pomace extract obtained by classical extraction.

Ovidius University Annals of Chemistry

Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry, 2017

It is well known that growth and morphogenesis of plant tissue cultures can be improved by small ... more It is well known that growth and morphogenesis of plant tissue cultures can be improved by small amounts of some organic compounds. Heterocyclic compounds such as chromanones and thiazoles derivatives, valuable because of their potential biological activities, have also been reported as pesticides, herbicides and plant-growth regulators. In the present study, different concentrations of chromanones and thiazoles derivatives were employed to evaluate their effects on plantlets growth of Ocimum basilicum L. and Echinacea purpurea L. The studied compounds were proved to be growth inhibitors at high concentrations. A growth stimulation effect was registered at low concentration.

Propagation of Ornamental Plants, 2009

International Journal of Molecular Sciences

Cell and tissue plant cultures are used either to save vulnerable species from extinction or to m... more Cell and tissue plant cultures are used either to save vulnerable species from extinction or to multiply valuable genotypes, or both, and are widely applied for economically important plant species. For medicinal plants, the use of in vitro technologies for the production of secondary metabolites and pathogen-free plants has been greatly developed. Two opposite aspects characterize the in vitro micropropagation of medicinal plants: maintaining genetic fidelity for the perpetuation and preservation of elites, and the identification and exploitation of somaclonal variations associated with new, useful traits. A balance between what is advantageous and what is undesirable is necessary, and this implies the identification of somaclonal variability at all levels, from the phenotypic to molecular ones. This review addresses the somaclonal variation arising from the in vitro multiplication of medicinal plants from three perspectives: cytogenetics, genetics, and epigenetics. The possible ca...

Applied Sciences

The present study is focused on mathematical modeling by testing the benefits of modified potato ... more The present study is focused on mathematical modeling by testing the benefits of modified potato starch in the biomass production of microorganisms, such as the fungus type. Microorganisms need a carbon source for the biomass development. In different industries, microorganisms, such as the Penicillium type, are used for the extraction of different important compounds utilized in biotechnologies. The aim of this study is to establish some important parameters in order to stimulate the biomass production in the presence of chemically modified starch. The carbon sources used in this research are glucose, native potato starch, and chemically modified potato starch. The chemical modification of potato starch was realized with green chemical compounds in order to not influence biomass development. The chemical characterization of starch and modified starch was important in order to confirm the chemical modification of starch. The response function in mathematical modeling is the amount o...

ANALELE STIINTIFICE ALE UNIVERSITATII "ALEXANDRU IOAN CUZA" DIN IASI SEC. II A. GENETIC... more ANALELE STIINTIFICE ALE UNIVERSITATII "ALEXANDRU IOAN CUZA" DIN IASI SEC. II A. GENETICA SI BIOLOGIE MOLECULARA