Inci Tuney | Ege University (original) (raw)

Papers by Inci Tuney

The International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences, 2016

Fresenius Environmental Bulletin

Copper is essential to living organisms, but at elevated concentrations, copper may become toxic ... more Copper is essential to living organisms, but at elevated concentrations, copper may become toxic for living sys- tems. During this research the effects of copper were evalu- ated at molecular levels in a marine green algae Ulva lac- tuca. In addition, the protection role of polyamines against DNA mutations and strand breaks against the copper treat- ment searched by RAPD-PCR analysis. The main changes observed in the RADP profiles have been resulted both in appearance or disappearance of different bands and varia- tion of their intensity.

Journal of photochemistry and photobiology. B, Biology, Jan 9, 2009

Nitszchia palea (Kutzing) W. Smith 1856 is a uni- cellular diatom which has a wide distribution a... more Nitszchia palea (Kutzing) W. Smith 1856 is a uni- cellular diatom which has a wide distribution all over the world, especially in fresh waters and soil. In our study freshwater Nitszchia palea community isolated from soil and growth in Diatom Medium (DM) in laboratory conditions with 24 hours illumination. Cells treated with cadmium acetate which is a highly toxic element and generally carried by industrial wastes to freshwater environ- ment. Cells exposed to 3 concentrations of cadmium acetate (0.1 mM, 1 mM, 5 mM and a con- trol group) for 7, 24 and 48 hours. After every treatment cells were prepared for scanning electron microscopy (SEM) and RAPD-PCR analysis (Random Amplified Polymorphic DNA- Polymerase Chain Reaction). The effect of cad- mium on frustule deformation was determined by SEM photography and the genetic deformation by RAPD-PCR. Dose dependent frustule deforma- tion was observed especially with 1mM and 5mM concentrations. Duration also effected the cell wall deformation. The RAPD technique was used to detect DNA damage. The main changes observed in the RAPD profiles resulted in both appearance and disappearance of different bands and variation of their intensity. In our opinion, cadmium affected the cell wall structure and it may be lead inhibition of silica biosynthesis on genetic levels.

Cystoseira sp. is one of the most widely distributed genera of the Fucales order and provides an ... more Cystoseira sp. is one of the most widely distributed genera of the Fucales order and provides an essential habitat for many epiphytes, invertebrates, and fish. Cystoseira sp. is found mostly in temperate regions of the Northern Hemisphere, such as the Mediterranean, Indian, and Pacific Oceans. Cystoseira sp. is characterized by highly differentiated basal and apical regions and presence of catenate pneumatocysts air-vesicles. Random Amplified Polymorphic DNA (RAPD-PCR) method is commonly used in toxicity, mutation detection and identification studies in macro and microalgae. Among PCR based studies it is widely recognised that RAPD-PCR is a rapid and reliable method for intra and inter-specific differentiation of most plant species. In this study RAPD method was used to determine genetic diversity between Cystoseria crinita (Desf.) Bory populations. 6 different populations of Cystoseria crinita (Desf.) Bory used as a material in the study. 11 different 10 bp RAPD-primers used and 8 of them gave acceptable bands. Some species identification keys for Cystoseira species are not generally give satisfying results. The Cystoseira species looks the same in some cases. Besides the morphological features are change according to seasons, enviromental conditions, life cycles and some enviromental effects. For these reasons molecular methods may be used to support morphological identification.

The purpose of this project was to investigate the total protein, sugar and phenolic content in p... more The purpose of this project was to investigate the total protein, sugar and phenolic content in phosphate extracts of marine macroalgae species from the Aegean Sea. Colpomenia sinuosa (Mertens ex Roth) Derbès & Solier, Petalonia fascia (O.F. Müller) Kuntze, Scytosiphon lomentaria (Lyngbye) Link., Dilophus spiralis (Montagne) G. Hamel, and Padina pavonia J.V. Lamouroux were collected from Izmir Bay during April, as well as Cystoseira sp. collected on the Urla, Bodrum, Fethiye and Kas coastlines during the spring and summer months. The phenolic content of five brown algae extracts varied from 2.5 mg to 21.8 mg GAE/10 g. Dilophus spiralis extracts demonstrated the lowest concentrations based on total protein assay, while Scytosiphon lomentaria extracts showed the highest sugar contents. The extract of Cystoseira sp. collected from Fethiye and Kas, gave the highest concentrations in May.

There are various methods published about DNA extraction from marine algae. These methods are th... more There are various methods published about DNA extraction from marine algae. These methods are the modifications of several DNA extraction methods from other organisms. Extraction of DNA from seaweeds are difficult processes because of the polysaccharide and polyphenole compounds of their thallus. In this study, DNA is extracted from a brown alga (Scytosiphon lomentaria and Cystoseira sp., Ectocarpus sp. ) collected from the Bay of Izmir by using modified CTAB (cetiltrimethylamonium bromide) protocol and used in PCR analysis. This modified method was also found efficient and applicaple for other molecular purposes.

rototheca is an aerobic, achlorophilic and unicellular organism with ovoid shape; it is often con... more rototheca is an aerobic, achlorophilic and unicellular organism with ovoid shape; it is often confused with yeasts. Prototheca cells are fairly common in a variety of enviro- ments such as sewage, freshwater, soil, rotting leaves, faeces, skin and various mammals (deer, cat, dog, cattle and humans). Prototheca species are widespread in housing areas, pens and pastures used by dairy cattle and can cause mastitis in dairy cattle. Protothecosis is an uncommon infection of humans and animals. Most infections are clinical and remain as chronic infections. Five species of Prototheca have been identified: P. moriformas, P. stagnora, P. ulmea, P. wickerhamii, P. zopfii; only the last two of these cause infection in humans and animals. Little is known about the ecology of Prototheca. Failure to isolate Prototheca spp. may be explained by the fact that they are readly over-grown by bacteria and fungi when culture is attemped from contaminated sources, as well as the fact that they superficially resemble yeasts. For this reason a specific culture media has been developed for Prototheca spp.

During this research, a total of 98 extracts from 13 al- gal species were tested in vitro for ant... more During this research, a total of 98 extracts from 13 al- gal species were tested in vitro for antimicrobial activities of algae against Candida sp., Enterococcus faecalis, Staphy- lococcus aureus, Streptococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli using the disc diffusion method. The ability of algae to perform antibacterial and antifungal activity was investigated at three different locali- ties. Cystoseira mediterranea and Ulva rigida were the only algae found in all three sites, and contamination was detected in algae collected from sites 1 and 3, with anti- bacterial capacity being highest in those from site 1, where samples were collected during red tide. Extractions were performed with four different solvents – ethanol, methanol, acetone and diethyl ether. In particular, diethyl ether ex- tracts of fresh C. mediterranea, Enteromorpha linza, U. rigida, Gracilaria gracilis and Ectocarpus siliculosus ap- peared to yield better results than those of methanol, etha- nol and acetone. However, diethyl ether extracts of some species, such as Padina pavonica, Colpomenia sniosa, Dic- tyota linearis, Dictyopteris membranacea, Ceramium ru- brum, and Acanthophora nojadiformis, gave different re- sults. Methanol extracts of A. nojadiformis showed effec- tive antibacterial activity against P. aeruginosa, and metha- nol extracts of C. rubrum showed antifungal and antibac- terial activity against Candida sp., E. coli, and P. aerugi- nosa. Acetone extracts of Hypnea sp. showed antibacterial activity against P. aeruginosa, and ethanol extract of Clado- phora sp. against E. faecalis only. Some of the collected samples were dried and extracts of fresh and dry samples were compared. Although fresh extracts of G. gracilis and E. siliculosus inhibited the tested microorganisms from populating, their dried extracts had no effect on gram- nega- tive and positive bacteria.

Methanol, acetone, diethyl ether, and ethanol extracts of 11 seaweed species from the coast of Ur... more Methanol, acetone, diethyl ether, and ethanol extracts of 11 seaweed species from the coast of Urla were tested in vitro for their antimicrobial activities against Candida sp., Enterococcus faecalis, Staphylococcus aureus, Streptococcus epidermidis, Pseudomonas aeruginosa, and Escherichia coli with the disc diffusion method. Diethyl ether was the best solution for extracting the effective antimicrobial materials from the algae species used in this experiment, with the exception of D. linearis, for which ethanol was the most effective extraction solution. Diethyl ether extracts of fresh Cystoseira mediterranea, Enteromorpha linza, Ulva rigida, Gracilaria gracilis, and Ectocarpus siliculosus showed effective results against all test organisms. However, diethyl ether extracts of some species, such as Padina pavonica, Colpomenia sniosa, Dictyota linearis, Dictyopteris membranacea, Ceramium rubrum, and Acanthophora nojadiformis, gave different results. A significant difference in antimicrobial activity was not observed between the acetone and methanol extracts of each alga. In addition, as a result of the comparison of dried and fresh extract antimicrobial activity, it was found that all test organisms were more sensitive to fresh extracts of the algae. Although fresh extracts of G. gracilis, D. linearis, and E. siliculosus inhibited the tested bacteria and yeast, their dried extracts had no inhibition activity on either Gram- negative or Gram-positive bacteria.

Journal of Photochemistry and Photobiology B-biology, 2009

Current Opinion in Biotechnology, 2011