Yigit UYANIKGIL | Ege University (original) (raw)
Papers by Yigit UYANIKGIL
Child's nervous system, Mar 13, 2024
Arsiv Kaynak Tarama Dergisi, Dec 30, 2022
Intraabdominal adhesions are an important health problem that is seen in the postoperative period... more Intraabdominal adhesions are an important health problem that is seen in the postoperative period and negatively affects the quality of life. Chemical and thermal factors that occur in the peritoneal cavity and serosal surfaces, causing abdominal trauma, or infection and foreign body reactions may cause adhesion formation. Although the classification of intraabdominal adhesions is generally based on adhesion density and severity of prognosis, there is not yet a worldwide accepted standard classification system. Intraabdominal adhesions show clinical reflections with negative consequences such as pain, infertility, prolonged hospital stay after surgery and economic burden. In conclusion, adhesions encountered in the postoperative period are a serious problem and further studies should be adapted from laboratory environment to clinical research models in order to prevent adhesion formation. This review was prepared to review the literature on intraabdominal adhesion formation, histopathology, grading, prevention and clinical significance.
Ege Tıp Dergisi, Mar 1, 2009
Giriş: Leptin, ob gen tarafından kodlanan 16-kDa ağırlığında bir moleküldür. Đlk olarak yağ dokus... more Giriş: Leptin, ob gen tarafından kodlanan 16-kDa ağırlığında bir moleküldür. Đlk olarak yağ dokusunda tanımlanan leptinin son zamanlarda vücutta çeşitli dokularda da ifade edildiği gözlenmiştir. Çeşitli çalışmalar leptinin midede de fonksiyon gösterebileceğini önermektedir. Bu çalışmada, sıçan mide dokusunda leptinin immünohistokimyasal dağılımını ve ifadesini araştırmak amaçlanmıştır. Gereç ve Yöntem: Bu çalışmada 190-210 gr ağırlığında erkek sıçanlar kullanılmıştır. Işık mikroskopik incelemeler için Wistar albino 5 sıçan anastezi edilmiş ve heparinize edilip fosfat tamponlu % 4 paraformaldehit ile intrakardiyak perfüze edilmiştir. Rutin histolojik takip uygulanan mide dokusu daha sonra 5 µm kalınlıkta kesilip Leptin immunohistokimyasal boyaması yapılmıştır. Bulgular: Midenin fundus bölgesinde leptin (+) boyanan gerek esas hücre, gerekse pariyetal hücre sayısı korpus bölgesine oranla anlamlı derecede artmış bulundu. Korpustaki leptin (+) hücreler kıyaslandığında esas hücre ve pariyetal hücre sayıları arasında anlamlı bir fark saptanmadı; diğer taraftan fundustaki leptin (+) esas hücre sayısının leptin (+) pariyetal hücre sayısından anlamlı şekilde fazla olduğu gözlendi. Ayrıca, myenterik pleksusta boyanma gözlemlendi. Sonuç:Bu çalışmada diğer çalışmalardan farklı olarak temel olarak gastrointestinal hareketleri kontrol eden myenterik pleksusda da leptinin varlığı gösterilmektedir. Leptinin sinerjistik etki gösterip beslenmenin düzenlenmesinde kısa süreli regülasyon ve/veya uzun süreli regülasyonda rol oynayabileceği gösterilmektedir.
Immunopharmacology and Immunotoxicology, Jun 9, 2020
Background: Human epidermal growth factor receptor 2 (HER2) is overexpressed in a subset of cance... more Background: Human epidermal growth factor receptor 2 (HER2) is overexpressed in a subset of cancers including 25% of breast cancers. Since combination therapy consisting of multiple therapeutic approaches is considered a promising regimen, we examined combination treatment modalities in a xenograft model in Balb/c mice injected with 4T1-HER2 cells. We used HER2/neu-loaded bone marrow-derived dendritic cells (BM-DC's) along with anti-PD-L1 monoclonal antibody in a new combination immunotherapy model. Methods: The combination was composed of an active immunotherapy (i.e. BM-DC-based vaccine) designed to boost the immune response against target antigen and was augmented by using anti-PD-L1 mAb to prevent immune evasion by the xenografted tumors. The vaccine combination was further supported using a QS-21 saponin adjuvant and the immune response was evaluated. Results: Mice treated with HER2/neu-loaded BM-DCs, combined with QS-21 and anti-PD-L1 mAb had significantly decreased tumor sizes and their splenocytes had enhanced cytotoxic activity, based on the lactate dehydrogenase (LDH) assay, compared to vaccine and adjuvant groups alone. The same vaccination group demonstrated a remarkable increase in IFN-c secreting CD8þ T-cells analyzed by flow cytometry. ELISA data also revealed a significant increase in the serum anti-HER2 IgG1 response; in addition, there was significant splenocyte proliferation upon stimulation with antigen compared to vaccine and adjuvant groups. Consistently, a significant infiltration of CD4þ, CD8þ immune cells in and around the tumors was observed. Conclusions: Our data suggest that the BM-DC þ HER2/neu þ QS-21 þ anti-PD-L1 vaccine combination paradigm synergistically generates anti-tumor activity and immune responses against HER2 overexpressing breast cancer in mice.
DOAJ (DOAJ: Directory of Open Access Journals), Jun 1, 2010
Cellular signaling pathways have important roles in cellular growth, differentiation, inflammator... more Cellular signaling pathways have important roles in cellular growth, differentiation, inflammatory response and apoptosis and in regulation of cellular responses under various chemical stimulators. Different proteins which belong to these pathways may be exposed to loss-of-function or gain-of-function mutations; this may lead to many clinical phenotypes including primarily cancer. In this review information about basic working principles of these pathways and diseases related to them are included.
Ege Tıp Dergisi, Dec 12, 2022
Aim: Mesenchymal stem cells can easily differentiate into cardiomyocytes in vitro conditions usin... more Aim: Mesenchymal stem cells can easily differentiate into cardiomyocytes in vitro conditions using various protocols. However, the agents used in these protocols have been reported to have some adverse effects on cell viability. Azacitidine is used to differentiate mesenchymal stem cells into cardiac muscle cells. The aim of the present study was to investigate the effects of Exenatide a GLP-1 receptor agonist, on differentiation and viability of human adipose tissue derived stem cells into cardiomyocytes. Materials and Methods: The effects of Azacytidine and Exenatide on cell viability and proliferation of human adipose tissue derived stem cells were analyzed with cytotoxicity assay. For differentiation procedure, of human adipose tissue derived stem cells were incubated with Azacytidine and Exenatide through four weeks. The morphological alterations of human adipose tissue derived stem cells were monitored and the expressions of cardiomyogenic differentiation markers (cTnI, GATA4 ve MYH7) were evaluated immunohistochemically. Also, cardiac troponin I (cTnI) levels in the cultures were measured using enzyme-linked immunosorbent assay. Results were evaluated by one way analysis of variance (ANOVA) and post-hoc test. Results: Treatment of the human adipose tissue derived stem cells with Azacytidine significantly decreased cell viability (54.4%) compared to control whereas treatment of cells with Azacytidine + Exenatide prevented cell death in a dose-dependent manner. Cells treated with Azacytidine and Exenatide showed significant morphological alterations consistent with cardiyomyogenic differentiation, and increase in expression cardiomyogenic markers. cTnI levels were found significantly higher in cultures treated separately and together with Azacytidine and Exenatide compared to control. Conclusion: Overall, these findings suggested that GLP-1 receptor agonist Exenatide may have beneficial effects on cardiomyogenic differention of human adipose tissue derived stem cells by reducing cell damage caused by Azacytidine.
Dicle Medical Journal, Sep 2, 2022
Objective: Mesenchymal stem cells (MSCs) are also promising in immunosuppressed patients after or... more Objective: Mesenchymal stem cells (MSCs) are also promising in immunosuppressed patients after organ and tissue transplantation, in addition to their current wide range of uses and research areas. Sunitinib is a receptor tyrosine kinase with immunosuppressive properties and its cytotoxic activity in different types of cells is known. Our study aimed to elucidate the effect of oxytocin on sunitinib-treated MSCs. Methods: For this purpose, commercially available rat adipose tissue-derived MSC (ADMSCs) was used. The individual or combinational effect of the active substances on viability was evaluated with WST-1, the effect on apoptosis Annexin V, the effect on oxidative stress markers MDA, CAT, GPX, and SOD ELISA tests. Results: The IC50 value of sunitinib was determined as 44.57 µM at the 48th hour, and it was determined that oxytocin had no cytotoxic effect in doses up to 100 µM. Treatment of the two agents in combination increased the cytotoxic effect of sunitinib. Oxytocin attenuated the effect of sunitinib on apoptosis and lipid peroxidation. Conclusion: It is important to investigate the efficacy of these two substances individually and in combination with ADMSCs with further experiments to evaluate the potential use of oxytocin in organ and tissue transplantations.
Ege Tıp Dergisi, Mar 15, 2022
The axolotl has an extraordinary capacity to regenerate damaged and lost structures, especially t... more The axolotl has an extraordinary capacity to regenerate damaged and lost structures, especially the nervous system, limbs, organs such as the eye and heart, without causing scarring. It has become a very important model organism by attracting the attention of scientists working in both developmental biology and regenerative medicine and stem cell biology. The axolotl, which is amphibian and tetrapod, is a more preferred model due to its ease of maintenance and reproduction compared to other organisms such as African clawed frog (Xenopus laevis) or zebrafish (Danio rerio), which are relatively difficult to study. The main purposes of this review are the definition and origin of the axolotl, its taxonomy, anatomy, reproduction, nutrition, habitat, to give a perspective to scientists who want to work on this model organism by giving examples to the scientific data and study fields of the last 20 years by addressing issues such as how it contributes to scientific studies as a model organism.
Dicle Medical Journal, Dec 15, 2019
Amaç: Bu çalışmada, bilinen bazı boyama yöntemlerinin modifiye edilerek stria longitudinalis medi... more Amaç: Bu çalışmada, bilinen bazı boyama yöntemlerinin modifiye edilerek stria longitudinalis medialis ve lateralis'in histo-morfolojik olarak incelenmesi ve elde edilecek bulguların bundan sonra yapılacak olan benzer çalışmalara ışık tutması amaçlanmıştır. Yöntemler: Çalışmamızda, %10'luk formaldehit ile tespit edilmiş, makroskobik patolojisi gözlenmeyen, doku bütünlüğü bozulmamış yetişkin kadavra beyin hemisferleri arasından elde edilen dokular kullanıldı. Beş kadavra beyninden alınan örneklerden üçü uygun boyama metoduna ulaşabilmek amacıyla kullanıldı. Doku fiksasyon metodu, Hematoksilen-eozin boyama metodu ve Luxol fast blue boyama metodu uygulandı. Bulgular: Histolojik olarak ilk defa corpus callosum (CC), indusium griseum (IG) ve stria longitudinalis (SL) aynı görüntü üzerinde tespit edildi. SL'in lifleri, vasküler ve morfolojik yapısı belirtildi. Sonuç: Umuyoruz ki, SL ile ilgili histolojik, immünohistokimyasal ve anatomik yaklaşımlar ışığında yaptığımız bu girişim, bu konu üzerindeki bazı karanlık yönleri aydınlatacaktır. Böylece bizden sonra SL üzerinde çalışacak araştırmacıların istifadesine sunulmak üzere akademik bir hafıza oluşturacaktır.
The present study was carried out to determine hemocyte types of medical leech, Hirudo medicinali... more The present study was carried out to determine hemocyte types of medical leech, Hirudo medicinalis. Four hemocytes types were identified; prohemocytes, plasmatocytes, granulocytes and eleocytes. They were characterized by light microscopy according to size, presence or absence of granules and nucleus/cytoplasm ratio. The prohemocytes were the smallest cells with large nuclei in the hemolymph. Plasmatocytes were polymorphic, varied from ovoid to spindle-shaped cells. Plasmatocytes were the most abundant hemocyte type in the hemolymph of H. medicinalis. Granulocytes were elliptical in shape and characterized by the presence of cytoplasmic granules. Eleocytes were spherical cells with homogeneous and slightly granular cytoplasm. The aim of this study was to characterise the hemocytes in H. medicinalis and to determine whether any differences from other invertebrates in terms of hemocyte types due to use in medical
Archives of Medical Science, Oct 22, 2009
Early developmental process of mammalian embryo is almost completely directed by the behavior of ... more Early developmental process of mammalian embryo is almost completely directed by the behavior of stem cells, which is controlled by both environmental and intrinsic factors. These cells commonly subject to dividing, migration, deterioration or death. Comparing to all other tissues in the body, central nervous system has a considerably limited capacity to regenerate. Recent knowledge on neural stem cells has brought novel approaches as to the use of stem cells in the treatment of some neurodegenerative disorders such as Parkinson, Alzheimer disease and amyotrophic lateral sclerosis, as well as in the management of spinal cord injuries. However, scientific literature requires detailed information regarding the proliferation and differentiation of stem cells and the mechanisms controlling the migration of these cells to the targeted central nervous system site. Development of new therapeutic protocols using stem cells and their effective clinical application in the future would bring light to cope with a number of systemic diseases, especially neurological disorders. This review has considered the biological features of stem cells, stem cell plasticity, potential application of stem cells in neurological diseases and cancer, highlighting the promises as well as the problems of this treatment approach.
PubMed, Sep 1, 2023
Background: In stem cell applications, apart from bone marrow and adipose tissue, compact bone is... more Background: In stem cell applications, apart from bone marrow and adipose tissue, compact bone is also used as an alternative. However, studies on this subject are limited. In our study, we investigated the effect of stem cell derived from compact bone on rat zygomatic arch defect. Methods: Fifteen rats were included in the study. Five rats were killed to obtain stem cells before the experiment. The rats were divided into 2 groups with 5 rats each. In group 1, compact bone-derived stem cell was applied. In group 2, adipose tissue-derived stem cell was applied. Right zygomatic arch defect was created in rats in both groups. Zygomatic bones were decellularized by cryosurgery. Stem cells were transferred to zygomatic bones. The number of stem cells, stem cell differentiation, and superficial markers obtained from the groups were examined. Histologically, cell structure, osteocyte count and osteopontin scores, elemental composition of the groups, percentages of resemblance to intact bone, osteocytes numbers, and cells were examined by electron microscopy of the bones in the groups after killing. Results: The number of stem cells administered to the groups was 5 × 107 and 3.2 × 107 for group 1 and group 2, respectively (P > 0.05). Histologically, the morphology of the cells in group 1 was found to be healthier than group 2. The number of osteocytes was 97.56 ± 15.4 and 132.93 ± 10.8 in group 1 and group 2, respectively (P < 0.05). The osteopontin score was 3.47 ± 0.73 and 65 ± 0.64 in group 1 and group 2, respectively (P < 0.05). In the electron microscope examination, the morphologies of the cells in group 1 were seen more normal. The Ca/P ratio of the groups was 1.51 and 1.59 in group 1 and group 2, respectively (P > 0.05). Osteocyte counts were 10.7 ± 2.8 and 6.1 ± 1.2 in group 1 and group 2, respectively (P < 0.05). Morphological similarity percentages to normal bone were 88.4% and 79.6% in group 1 and group 2, respectively (P > 0.05). Conclusion: Stem cells obtained from compact bone gave positive results in zygomatic arch defect. This method can also be used as an alternative in stem cell applications.
Journal of cancer science and research, 2018
Objective: Caffeine (CAF), which is in the methylxanthines group (1,3,7-trimethylxanthine), is a ... more Objective: Caffeine (CAF), which is in the methylxanthines group (1,3,7-trimethylxanthine), is a neurologically active food component that is widely consumed and has a stimulating effect on the central nervous system. CAF taken in high doses during pregnancy rapidly crosses the placenta and causes many negative conditions such as low birth weight infants, premature births, spontaneous abortion, stillbirth, and principally fetal growth retardation. On the other hand, melatonin (MEL) is an endogenous hormone secreted from the pineal gland that plays a role in the regulation of many biological functions such as sleep, biological rhythm, reproduction, immunity and has neuroprotective effects. In this study, we aimed to investigate the possible effects of exogenous MEL on the fetal hippocampus damage caused by high-dose CAF administration in pregnant rats. Methods: In the study, 32 adult Wistar albino female rats were divided into four experimental groups after conception (n=8). No compo...
Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi
The aim of this study was to investigate the effects of prenatal stress (PS) on mRNA levels of DN... more The aim of this study was to investigate the effects of prenatal stress (PS) on mRNA levels of DNA methyltransferases (DNMTs) and histone deacetylases (HDACs) in cerebral cortex and hippocampus of female rats. PS was induced in rats with dexamethasone (Dex). From gestation day 14 to 21, pregnant rats were injected daily with Dex (100 μg/kg) or saline. After birth, at 3 months of age, female rats were decapitated (n=5). The effects of Dex on epigenetic mechanisms were investigated by real-time PCR through mRNA levels of DNMT1, DNMT3a, DNMT3b, HDAC1 and HDAC2. Statistical significant differences were determined with one-way analysis of variance. Prenatal Dex exposure caused significant increases in DNMT3a, HDAC1 and HDAC2 mRNA levels in cortex and hippocampus. We further found that DNMT3b mRNA levels significantly increased in hippocampus but decreased in cortex of Dex group. No significant differences were found in DNMT1 mRNA levels. It was concluded that PS may trigger dysregulation...
Child's nervous system, Mar 13, 2024
Arsiv Kaynak Tarama Dergisi, Dec 30, 2022
Intraabdominal adhesions are an important health problem that is seen in the postoperative period... more Intraabdominal adhesions are an important health problem that is seen in the postoperative period and negatively affects the quality of life. Chemical and thermal factors that occur in the peritoneal cavity and serosal surfaces, causing abdominal trauma, or infection and foreign body reactions may cause adhesion formation. Although the classification of intraabdominal adhesions is generally based on adhesion density and severity of prognosis, there is not yet a worldwide accepted standard classification system. Intraabdominal adhesions show clinical reflections with negative consequences such as pain, infertility, prolonged hospital stay after surgery and economic burden. In conclusion, adhesions encountered in the postoperative period are a serious problem and further studies should be adapted from laboratory environment to clinical research models in order to prevent adhesion formation. This review was prepared to review the literature on intraabdominal adhesion formation, histopathology, grading, prevention and clinical significance.
Ege Tıp Dergisi, Mar 1, 2009
Giriş: Leptin, ob gen tarafından kodlanan 16-kDa ağırlığında bir moleküldür. Đlk olarak yağ dokus... more Giriş: Leptin, ob gen tarafından kodlanan 16-kDa ağırlığında bir moleküldür. Đlk olarak yağ dokusunda tanımlanan leptinin son zamanlarda vücutta çeşitli dokularda da ifade edildiği gözlenmiştir. Çeşitli çalışmalar leptinin midede de fonksiyon gösterebileceğini önermektedir. Bu çalışmada, sıçan mide dokusunda leptinin immünohistokimyasal dağılımını ve ifadesini araştırmak amaçlanmıştır. Gereç ve Yöntem: Bu çalışmada 190-210 gr ağırlığında erkek sıçanlar kullanılmıştır. Işık mikroskopik incelemeler için Wistar albino 5 sıçan anastezi edilmiş ve heparinize edilip fosfat tamponlu % 4 paraformaldehit ile intrakardiyak perfüze edilmiştir. Rutin histolojik takip uygulanan mide dokusu daha sonra 5 µm kalınlıkta kesilip Leptin immunohistokimyasal boyaması yapılmıştır. Bulgular: Midenin fundus bölgesinde leptin (+) boyanan gerek esas hücre, gerekse pariyetal hücre sayısı korpus bölgesine oranla anlamlı derecede artmış bulundu. Korpustaki leptin (+) hücreler kıyaslandığında esas hücre ve pariyetal hücre sayıları arasında anlamlı bir fark saptanmadı; diğer taraftan fundustaki leptin (+) esas hücre sayısının leptin (+) pariyetal hücre sayısından anlamlı şekilde fazla olduğu gözlendi. Ayrıca, myenterik pleksusta boyanma gözlemlendi. Sonuç:Bu çalışmada diğer çalışmalardan farklı olarak temel olarak gastrointestinal hareketleri kontrol eden myenterik pleksusda da leptinin varlığı gösterilmektedir. Leptinin sinerjistik etki gösterip beslenmenin düzenlenmesinde kısa süreli regülasyon ve/veya uzun süreli regülasyonda rol oynayabileceği gösterilmektedir.
Immunopharmacology and Immunotoxicology, Jun 9, 2020
Background: Human epidermal growth factor receptor 2 (HER2) is overexpressed in a subset of cance... more Background: Human epidermal growth factor receptor 2 (HER2) is overexpressed in a subset of cancers including 25% of breast cancers. Since combination therapy consisting of multiple therapeutic approaches is considered a promising regimen, we examined combination treatment modalities in a xenograft model in Balb/c mice injected with 4T1-HER2 cells. We used HER2/neu-loaded bone marrow-derived dendritic cells (BM-DC's) along with anti-PD-L1 monoclonal antibody in a new combination immunotherapy model. Methods: The combination was composed of an active immunotherapy (i.e. BM-DC-based vaccine) designed to boost the immune response against target antigen and was augmented by using anti-PD-L1 mAb to prevent immune evasion by the xenografted tumors. The vaccine combination was further supported using a QS-21 saponin adjuvant and the immune response was evaluated. Results: Mice treated with HER2/neu-loaded BM-DCs, combined with QS-21 and anti-PD-L1 mAb had significantly decreased tumor sizes and their splenocytes had enhanced cytotoxic activity, based on the lactate dehydrogenase (LDH) assay, compared to vaccine and adjuvant groups alone. The same vaccination group demonstrated a remarkable increase in IFN-c secreting CD8þ T-cells analyzed by flow cytometry. ELISA data also revealed a significant increase in the serum anti-HER2 IgG1 response; in addition, there was significant splenocyte proliferation upon stimulation with antigen compared to vaccine and adjuvant groups. Consistently, a significant infiltration of CD4þ, CD8þ immune cells in and around the tumors was observed. Conclusions: Our data suggest that the BM-DC þ HER2/neu þ QS-21 þ anti-PD-L1 vaccine combination paradigm synergistically generates anti-tumor activity and immune responses against HER2 overexpressing breast cancer in mice.
DOAJ (DOAJ: Directory of Open Access Journals), Jun 1, 2010
Cellular signaling pathways have important roles in cellular growth, differentiation, inflammator... more Cellular signaling pathways have important roles in cellular growth, differentiation, inflammatory response and apoptosis and in regulation of cellular responses under various chemical stimulators. Different proteins which belong to these pathways may be exposed to loss-of-function or gain-of-function mutations; this may lead to many clinical phenotypes including primarily cancer. In this review information about basic working principles of these pathways and diseases related to them are included.
Ege Tıp Dergisi, Dec 12, 2022
Aim: Mesenchymal stem cells can easily differentiate into cardiomyocytes in vitro conditions usin... more Aim: Mesenchymal stem cells can easily differentiate into cardiomyocytes in vitro conditions using various protocols. However, the agents used in these protocols have been reported to have some adverse effects on cell viability. Azacitidine is used to differentiate mesenchymal stem cells into cardiac muscle cells. The aim of the present study was to investigate the effects of Exenatide a GLP-1 receptor agonist, on differentiation and viability of human adipose tissue derived stem cells into cardiomyocytes. Materials and Methods: The effects of Azacytidine and Exenatide on cell viability and proliferation of human adipose tissue derived stem cells were analyzed with cytotoxicity assay. For differentiation procedure, of human adipose tissue derived stem cells were incubated with Azacytidine and Exenatide through four weeks. The morphological alterations of human adipose tissue derived stem cells were monitored and the expressions of cardiomyogenic differentiation markers (cTnI, GATA4 ve MYH7) were evaluated immunohistochemically. Also, cardiac troponin I (cTnI) levels in the cultures were measured using enzyme-linked immunosorbent assay. Results were evaluated by one way analysis of variance (ANOVA) and post-hoc test. Results: Treatment of the human adipose tissue derived stem cells with Azacytidine significantly decreased cell viability (54.4%) compared to control whereas treatment of cells with Azacytidine + Exenatide prevented cell death in a dose-dependent manner. Cells treated with Azacytidine and Exenatide showed significant morphological alterations consistent with cardiyomyogenic differentiation, and increase in expression cardiomyogenic markers. cTnI levels were found significantly higher in cultures treated separately and together with Azacytidine and Exenatide compared to control. Conclusion: Overall, these findings suggested that GLP-1 receptor agonist Exenatide may have beneficial effects on cardiomyogenic differention of human adipose tissue derived stem cells by reducing cell damage caused by Azacytidine.
Dicle Medical Journal, Sep 2, 2022
Objective: Mesenchymal stem cells (MSCs) are also promising in immunosuppressed patients after or... more Objective: Mesenchymal stem cells (MSCs) are also promising in immunosuppressed patients after organ and tissue transplantation, in addition to their current wide range of uses and research areas. Sunitinib is a receptor tyrosine kinase with immunosuppressive properties and its cytotoxic activity in different types of cells is known. Our study aimed to elucidate the effect of oxytocin on sunitinib-treated MSCs. Methods: For this purpose, commercially available rat adipose tissue-derived MSC (ADMSCs) was used. The individual or combinational effect of the active substances on viability was evaluated with WST-1, the effect on apoptosis Annexin V, the effect on oxidative stress markers MDA, CAT, GPX, and SOD ELISA tests. Results: The IC50 value of sunitinib was determined as 44.57 µM at the 48th hour, and it was determined that oxytocin had no cytotoxic effect in doses up to 100 µM. Treatment of the two agents in combination increased the cytotoxic effect of sunitinib. Oxytocin attenuated the effect of sunitinib on apoptosis and lipid peroxidation. Conclusion: It is important to investigate the efficacy of these two substances individually and in combination with ADMSCs with further experiments to evaluate the potential use of oxytocin in organ and tissue transplantations.
Ege Tıp Dergisi, Mar 15, 2022
The axolotl has an extraordinary capacity to regenerate damaged and lost structures, especially t... more The axolotl has an extraordinary capacity to regenerate damaged and lost structures, especially the nervous system, limbs, organs such as the eye and heart, without causing scarring. It has become a very important model organism by attracting the attention of scientists working in both developmental biology and regenerative medicine and stem cell biology. The axolotl, which is amphibian and tetrapod, is a more preferred model due to its ease of maintenance and reproduction compared to other organisms such as African clawed frog (Xenopus laevis) or zebrafish (Danio rerio), which are relatively difficult to study. The main purposes of this review are the definition and origin of the axolotl, its taxonomy, anatomy, reproduction, nutrition, habitat, to give a perspective to scientists who want to work on this model organism by giving examples to the scientific data and study fields of the last 20 years by addressing issues such as how it contributes to scientific studies as a model organism.
Dicle Medical Journal, Dec 15, 2019
Amaç: Bu çalışmada, bilinen bazı boyama yöntemlerinin modifiye edilerek stria longitudinalis medi... more Amaç: Bu çalışmada, bilinen bazı boyama yöntemlerinin modifiye edilerek stria longitudinalis medialis ve lateralis'in histo-morfolojik olarak incelenmesi ve elde edilecek bulguların bundan sonra yapılacak olan benzer çalışmalara ışık tutması amaçlanmıştır. Yöntemler: Çalışmamızda, %10'luk formaldehit ile tespit edilmiş, makroskobik patolojisi gözlenmeyen, doku bütünlüğü bozulmamış yetişkin kadavra beyin hemisferleri arasından elde edilen dokular kullanıldı. Beş kadavra beyninden alınan örneklerden üçü uygun boyama metoduna ulaşabilmek amacıyla kullanıldı. Doku fiksasyon metodu, Hematoksilen-eozin boyama metodu ve Luxol fast blue boyama metodu uygulandı. Bulgular: Histolojik olarak ilk defa corpus callosum (CC), indusium griseum (IG) ve stria longitudinalis (SL) aynı görüntü üzerinde tespit edildi. SL'in lifleri, vasküler ve morfolojik yapısı belirtildi. Sonuç: Umuyoruz ki, SL ile ilgili histolojik, immünohistokimyasal ve anatomik yaklaşımlar ışığında yaptığımız bu girişim, bu konu üzerindeki bazı karanlık yönleri aydınlatacaktır. Böylece bizden sonra SL üzerinde çalışacak araştırmacıların istifadesine sunulmak üzere akademik bir hafıza oluşturacaktır.
The present study was carried out to determine hemocyte types of medical leech, Hirudo medicinali... more The present study was carried out to determine hemocyte types of medical leech, Hirudo medicinalis. Four hemocytes types were identified; prohemocytes, plasmatocytes, granulocytes and eleocytes. They were characterized by light microscopy according to size, presence or absence of granules and nucleus/cytoplasm ratio. The prohemocytes were the smallest cells with large nuclei in the hemolymph. Plasmatocytes were polymorphic, varied from ovoid to spindle-shaped cells. Plasmatocytes were the most abundant hemocyte type in the hemolymph of H. medicinalis. Granulocytes were elliptical in shape and characterized by the presence of cytoplasmic granules. Eleocytes were spherical cells with homogeneous and slightly granular cytoplasm. The aim of this study was to characterise the hemocytes in H. medicinalis and to determine whether any differences from other invertebrates in terms of hemocyte types due to use in medical
Archives of Medical Science, Oct 22, 2009
Early developmental process of mammalian embryo is almost completely directed by the behavior of ... more Early developmental process of mammalian embryo is almost completely directed by the behavior of stem cells, which is controlled by both environmental and intrinsic factors. These cells commonly subject to dividing, migration, deterioration or death. Comparing to all other tissues in the body, central nervous system has a considerably limited capacity to regenerate. Recent knowledge on neural stem cells has brought novel approaches as to the use of stem cells in the treatment of some neurodegenerative disorders such as Parkinson, Alzheimer disease and amyotrophic lateral sclerosis, as well as in the management of spinal cord injuries. However, scientific literature requires detailed information regarding the proliferation and differentiation of stem cells and the mechanisms controlling the migration of these cells to the targeted central nervous system site. Development of new therapeutic protocols using stem cells and their effective clinical application in the future would bring light to cope with a number of systemic diseases, especially neurological disorders. This review has considered the biological features of stem cells, stem cell plasticity, potential application of stem cells in neurological diseases and cancer, highlighting the promises as well as the problems of this treatment approach.
PubMed, Sep 1, 2023
Background: In stem cell applications, apart from bone marrow and adipose tissue, compact bone is... more Background: In stem cell applications, apart from bone marrow and adipose tissue, compact bone is also used as an alternative. However, studies on this subject are limited. In our study, we investigated the effect of stem cell derived from compact bone on rat zygomatic arch defect. Methods: Fifteen rats were included in the study. Five rats were killed to obtain stem cells before the experiment. The rats were divided into 2 groups with 5 rats each. In group 1, compact bone-derived stem cell was applied. In group 2, adipose tissue-derived stem cell was applied. Right zygomatic arch defect was created in rats in both groups. Zygomatic bones were decellularized by cryosurgery. Stem cells were transferred to zygomatic bones. The number of stem cells, stem cell differentiation, and superficial markers obtained from the groups were examined. Histologically, cell structure, osteocyte count and osteopontin scores, elemental composition of the groups, percentages of resemblance to intact bone, osteocytes numbers, and cells were examined by electron microscopy of the bones in the groups after killing. Results: The number of stem cells administered to the groups was 5 × 107 and 3.2 × 107 for group 1 and group 2, respectively (P > 0.05). Histologically, the morphology of the cells in group 1 was found to be healthier than group 2. The number of osteocytes was 97.56 ± 15.4 and 132.93 ± 10.8 in group 1 and group 2, respectively (P < 0.05). The osteopontin score was 3.47 ± 0.73 and 65 ± 0.64 in group 1 and group 2, respectively (P < 0.05). In the electron microscope examination, the morphologies of the cells in group 1 were seen more normal. The Ca/P ratio of the groups was 1.51 and 1.59 in group 1 and group 2, respectively (P > 0.05). Osteocyte counts were 10.7 ± 2.8 and 6.1 ± 1.2 in group 1 and group 2, respectively (P < 0.05). Morphological similarity percentages to normal bone were 88.4% and 79.6% in group 1 and group 2, respectively (P > 0.05). Conclusion: Stem cells obtained from compact bone gave positive results in zygomatic arch defect. This method can also be used as an alternative in stem cell applications.
Journal of cancer science and research, 2018
Objective: Caffeine (CAF), which is in the methylxanthines group (1,3,7-trimethylxanthine), is a ... more Objective: Caffeine (CAF), which is in the methylxanthines group (1,3,7-trimethylxanthine), is a neurologically active food component that is widely consumed and has a stimulating effect on the central nervous system. CAF taken in high doses during pregnancy rapidly crosses the placenta and causes many negative conditions such as low birth weight infants, premature births, spontaneous abortion, stillbirth, and principally fetal growth retardation. On the other hand, melatonin (MEL) is an endogenous hormone secreted from the pineal gland that plays a role in the regulation of many biological functions such as sleep, biological rhythm, reproduction, immunity and has neuroprotective effects. In this study, we aimed to investigate the possible effects of exogenous MEL on the fetal hippocampus damage caused by high-dose CAF administration in pregnant rats. Methods: In the study, 32 adult Wistar albino female rats were divided into four experimental groups after conception (n=8). No compo...
Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi
The aim of this study was to investigate the effects of prenatal stress (PS) on mRNA levels of DN... more The aim of this study was to investigate the effects of prenatal stress (PS) on mRNA levels of DNA methyltransferases (DNMTs) and histone deacetylases (HDACs) in cerebral cortex and hippocampus of female rats. PS was induced in rats with dexamethasone (Dex). From gestation day 14 to 21, pregnant rats were injected daily with Dex (100 μg/kg) or saline. After birth, at 3 months of age, female rats were decapitated (n=5). The effects of Dex on epigenetic mechanisms were investigated by real-time PCR through mRNA levels of DNMT1, DNMT3a, DNMT3b, HDAC1 and HDAC2. Statistical significant differences were determined with one-way analysis of variance. Prenatal Dex exposure caused significant increases in DNMT3a, HDAC1 and HDAC2 mRNA levels in cortex and hippocampus. We further found that DNMT3b mRNA levels significantly increased in hippocampus but decreased in cortex of Dex group. No significant differences were found in DNMT1 mRNA levels. It was concluded that PS may trigger dysregulation...