Alain Efstratiou | National and Kapodistrian University of Athens (original) (raw)
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Papers by Alain Efstratiou
Catheterization and Cardiovascular Diagnosis, 1994
A young woman who survived after suffering in the immediate postpartum period a spontaneous coron... more A young woman who survived after suffering in the immediate postpartum period a spontaneous coronary dissection with resultant myocardial infarction and a pulmonary embolism with pulmonary infarct is presented. Diagnostic and therapeutic considerations for this unique occurrence are discussed and reviewed.
Advances in Experimental Medicine and Biology, 1997
Microbiology, 1998
The genetic diversity of clinical isolates of Streptococcus pyogenes serotype M 5 has been charac... more The genetic diversity of clinical isolates of Streptococcus pyogenes serotype M 5 has been characterized. Strain genotypes were defined by macrorestriction profile, 165 ribotype, emm gene subtype, insertion element IS7239 profile, and exotoxin gene determinant. By these criteria, clinical isolates of M 5 constituted a multiplicity of strain clusters rather than a homogeneous population as found for certain serotypes. Distance matrices and an unrooted tree were constructed from macrorestriction data with three rarely cutting endonucleases, determined by PFGE. A single IS7239 profile was common to 85% of isolates but there was great diversity of both ribotype and macrorestriction profile, and 18 different emm gene subtypes were detected by PCR-RFLP. DNA sequence analysis of the antigen-coding 5' (hypervariable) region of emm gene amplicons (about 240 bp) showed that 14/18 exhibited up to 6 % divergence. Four amplicons had highly divergent sequencescorresponding to those previously determined for emm6, emm 11, emm 18 and emm77. Further serological and hybridization studies were used to analyse the discrepancy between the Lancef ield serotype of these strains (M5) and their emm genotype. Overall, this study shows a high degree of genetic diversity in serotype M5, with implications for the Lancefield scheme itself, for the epidemiology of group A streptococci, and for recombinant DNA strategies for M protein-based vaccine development. UK
Journal of the American College of Cardiology, 1995
Epidemiology and Infection, 2009
Journal of The American College of Cardiology, 1996
The American Journal of Cardiology, 1996
Catheterization and Cardiovascular Diagnosis, 1994
A young woman who survived after suffering in the immediate postpartum period a spontaneous coron... more A young woman who survived after suffering in the immediate postpartum period a spontaneous coronary dissection with resultant myocardial infarction and a pulmonary embolism with pulmonary infarct is presented. Diagnostic and therapeutic considerations for this unique occurrence are discussed and reviewed.
Advances in Experimental Medicine and Biology, 1997
Microbiology, 1998
The genetic diversity of clinical isolates of Streptococcus pyogenes serotype M 5 has been charac... more The genetic diversity of clinical isolates of Streptococcus pyogenes serotype M 5 has been characterized. Strain genotypes were defined by macrorestriction profile, 165 ribotype, emm gene subtype, insertion element IS7239 profile, and exotoxin gene determinant. By these criteria, clinical isolates of M 5 constituted a multiplicity of strain clusters rather than a homogeneous population as found for certain serotypes. Distance matrices and an unrooted tree were constructed from macrorestriction data with three rarely cutting endonucleases, determined by PFGE. A single IS7239 profile was common to 85% of isolates but there was great diversity of both ribotype and macrorestriction profile, and 18 different emm gene subtypes were detected by PCR-RFLP. DNA sequence analysis of the antigen-coding 5' (hypervariable) region of emm gene amplicons (about 240 bp) showed that 14/18 exhibited up to 6 % divergence. Four amplicons had highly divergent sequencescorresponding to those previously determined for emm6, emm 11, emm 18 and emm77. Further serological and hybridization studies were used to analyse the discrepancy between the Lancef ield serotype of these strains (M5) and their emm genotype. Overall, this study shows a high degree of genetic diversity in serotype M5, with implications for the Lancefield scheme itself, for the epidemiology of group A streptococci, and for recombinant DNA strategies for M protein-based vaccine development. UK
Journal of the American College of Cardiology, 1995
Epidemiology and Infection, 2009
Journal of The American College of Cardiology, 1996
The American Journal of Cardiology, 1996