Host-associated niche metabolism controls enteric infection through fine-tuning the regulation of type 3 secretion (original) (raw)
Connolly, J. P.R. et al. (2018) Host-associated niche metabolism controls enteric infection through fine-tuning the regulation of type 3 secretion.Nature Communications, 9, 4187. (doi: 10.1038/s41467-018-06701-4) (PMID:30305622) (PMCID:PMC6180029)
Abstract
Niche-adaptation of a bacterial pathogen hinges on the ability to recognize the complexity of signals from the environment and integrate that information with the regulation of genes critical for infection. Here we report the transcriptome of the attaching and effacing pathogen Citrobacter rodentium during infection of its natural murine host. Pathogen gene expression in vivo was heavily biased towards the virulence factor repertoire and was found to be co-ordinated uniquely in response to the host. Concordantly, we identified the host-specific induction of a metabolic pathway that overlapped with the regulation of virulence. The essential type 3 secretion system and an associated suite of distinct effectors were found to be modulated co-ordinately through a unique mechanism involving metabolism of microbiota-derived 1,2-propanediol, which dictated the ability to colonize the host effectively. This study provides novel insights into how host-specific metabolic adaptation acts as a cue to fine-tune virulence.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work was supported by a Wellcome Trust Investigator award to Gad Frankel (107057/Z/15/Z), Wellcome Trust ISSF grants (105614/Z/14/Z and 204820/Z/16/Z) and BBSRC funding (BB/M029646/1 & BB/R006539/1) awarded to A.J.R. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Connolly, Dr James and Douce, Dr Gillian and Roe, Professor Andrew and Herzyk, Dr Pawel and O'Boyle, Dr Nicky |
| Authors: | Connolly, J. P.R., Slater, S. L., O'Boyle, N., Goldstone, R. J., Crepin, V. F., Ruano-Gallego, D., Herzyk, P., Smith, D. G. E., Douce, G. R., Frankel, G., and Roe, A. J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & ImmunityCollege of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | Nature Communications |
| Publisher: | Nature Research |
| ISSN: | 2041-1723 |
| ISSN (Online): | 2041-1723 |
| Published Online: | 10 October 2018 |
| Copyright Holders: | Copyright © 2018 The Authors |
| First Published: | First published in Nature Communications 9:4187 |
| Publisher Policy: | Reproduced under a Creative Commons license |
University Staff: Request a correction | Enlighten Editors: Update this record
Funder and Project Information
1
Institutional Strategic Support Fund (2016)
Anna Dominiczak
204820/Z/16/Z
RI CARDIOVASCULAR & MEDICAL SCIENCES
3
Institutional Strategic Support Fund (ISSF)
Anna Dominiczak
105614/Z/14/Z
RI CARDIOVASCULAR & MEDICAL SCIENCES
1
Where and Why: The Influence of Host Metabolism on Bacterial Niche Specificity
Andrew Roe
BB/M029646/1
III - BACTERIOLOGY
0
The Role of Dietary D-serine in Health and Disease
Andrew Roe
BB/R006539/1
III - Bacteriology
Deposit and Record Details
| ID Code: | 195717 |
|---|---|
| Depositing User: | Ms Rachael Briggs |
| Datestamp: | 11 Sep 2019 10:46 |
| Last Modified: | 28 May 2020 22:53 |
| Date of acceptance: | 14 September 2018 |
| Date of first online publication: | 10 October 2018 |
| Date Deposited: | 11 September 2019 |