"Frozen evolution" of an RNA virus suggests accidental release as a potential cause of arbovirus re-emergence (original) (raw)

Pascall, D. J. et al. (2020) "Frozen evolution" of an RNA virus suggests accidental release as a potential cause of arbovirus re-emergence.PLoS Biology, 18(4), e3000673. (doi: 10.1371/journal.pbio.3000673) (PMID:32343693) (PMCID:PMC7188197)

Abstract

The mechanisms underlying virus emergence are rarely well understood, making the appearance of outbreaks largely unpredictable. Bluetongue virus serotype 8 (BTV-8), an arthropod-borne virus of ruminants, emerged in livestock in northern Europe in 2006, spreading to most European countries by 2009 and causing losses of billions of euros. Although the outbreak was successfully controlled through vaccination by early 2010, puzzlingly, a closely related BTV-8 strain re-emerged in France in 2015, triggering a second outbreak that is still ongoing. The origin of this virus and the mechanisms underlying its re-emergence are unknown. Here, we performed phylogenetic analyses of 164 whole BTV-8 genomes sampled throughout the two outbreaks. We demonstrate consistent clock-like virus evolution during both epizootics but found negligible evolutionary change between them. We estimate that the ancestor of the second outbreak dates from the height of the first outbreak in 2008. This implies that the virus had not been replicating for multiple years prior to its re-emergence in 2015. Given the absence of any known natural mechanism that could explain BTV-8 persistence over this long period without replication, we hypothesise that the second outbreak could have been initiated by accidental exposure of livestock to frozen material contaminated with virus from approximately 2008. Our work highlights new targets for pathogen surveillance programmes in livestock and illustrates the power of genomic epidemiology to identify pathways of infectious disease emergence.

Item Type: Articles
Status: Published
Refereed: Yes
Glasgow Author(s) Enlighten ID: Singer, Dr Josh and Palmarini, Professor Massimo and Da Silva Filipe, Dr Ana and Biek, Professor Roman and Mertens, Professor Peter and Nomikou, Dr Kyriaki and Pascall, Dr David and Jacquot, Dr Maude
Creator Roles: Pascall, D. J.Data curation, Formal analysis, Investigation, Methodology, Visualization, Writing – original draft, Writing – review and editingNomikou, K.Data curation, Formal analysis, Investigation, Methodology, Project administration, Writing – review and editingda Silva Filipe, A.Investigation, Methodology, Writing – review and editingJacquot, M.Investigation, Methodology, Writing – review and editingSinger, J. B.Software, Writing – review and editingPalmarini, M.Conceptualization, Funding acquisition, Project administration, Supervision, Writing – original draft, Writing – review and editing
Authors: Pascall, D. J., Nomikou, K., Breard, E., Zientara, S., Da Silva Filipe, A., Hoffmann, B., Jacquot, M., Singer, J. B., De Clercq, K., Bøtner, A., Sailleau, C., Viarouge, C., Batten, C., Puggioni, G., Ligio, C., Savini, G., van Rijn, P. A., Mertens, P. P.C., Biek, R., and Palmarini, M.
College/School: College of Medical Veterinary and Life Sciences > School of Infection & ImmunityCollege of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus ResearchCollege of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
Journal Name: PLoS Biology
Publisher: Public Library of Science
ISSN: 1544-9173
ISSN (Online): 1545-7885
Copyright Holders: Copyright © 2020 The Authors
First Published: First published in PLoS Biology First published in PLoS Biology 18(4): e3000673
Publisher Policy: Reproduced under a Creative Commons License

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Funder and Project Information

PALE-Blu

Roman Biek

Biek, Dr Roman

Institute of Biodiversity, Animal Health and Comparative Medicine

Phylogeography, transmission dynamics and pathogenesis of bluetongue

Massimo Palmarini

092806/Z/10/Z

III - Centre for Virus Research

Host determinants of disease outcomes in arboviral infections

Massimo Palmarini

206369/Z/17/Z

III - Centre for Virus Research

Deposit and Record Details

ID Code: 214032
Depositing User: Dr Mary Donaldson
Datestamp: 29 Jun 2020 13:21
Last Modified: 29 Oct 2024 15:31
Date of acceptance: 24 March 2020
Date of first online publication: 28 April 2020
Date Deposited: 29 June 2020
Data Availability Statement: Yes