Enhancing a multi-purpose artificial urine for culture and gene expression studies of uropathogenic Escherichia coli strains (original) (raw)
Rimbi, Patricia T., O'Boyle, Nicky, Douce, Gillian R. 
ORCID: https://orcid.org/0000-0002-6654-7346, Pizza, Mariagrazia, Rosini, Roberto and Roe, Andrew J. ORCID: https://orcid.org/0000-0003-3698-6134(2024) Enhancing a multi-purpose artificial urine for culture and gene expression studies of uropathogenic Escherichia coli strains.Journal of Applied Microbiology, 135(4), lxae067. (doi: 10.1093/jambio/lxae067) (PMID:38486355)
Abstract
Aims: The main objective of this study was to modify a recently reported multi-purpose artificial urine (MP-AU) for culture and gene expression studies of uropathogenic Escherichia coli (UPEC) strains. Methods and results: We used liquid chromatography mass spectrometry (LC-MS) to identify and adjust the metabolic profile of MP-AU closer to that of pooled human urine (PHU). Modification in this way facilitated growth of UPEC strains with growth rates similar to those obtained in PHU. Transcriptomic analysis of UPEC strains cultured in enhanced artificial urine (enhanced AU) and PHU showed that the gene expression profiles are similar, with less than 7% of genes differentially expressed between the two conditions. Conclusions: Enhancing an MP-AU with metabolites identified in PHU allows the enhanced AU to be used as a substitute for the culture and in vitro gene expression studies of UPEC strains.
| Item Type: | Articles |
|---|---|
| Additional Information: | PTR was funded by a University of Glasgow PhD studentship in collaboration with GSK. NOB was supported by the Biotechnology and Biological Sciences Research Council [BB/R006539/1]. GRD and AJR were supported by the Biotechnology and Biological Sciences Research Council [BB/W015781/1]. AJR was also supported by the Medical Research Council [MR/V011499/1]. |
| Keywords: | artificial urine, uropathogen, E. coli, transcriptome. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Roe, Professor Andrew and Douce, Dr Gillian and Rimbi, Patricia |
| Creator Roles: | Rimbi, P.Formal analysis, Investigation, Methodology, Visualization, Writing – original draft, Writing – review and editingDouce, G.Conceptualization, Funding acquisition, Methodology, Project administration, SupervisionRoe, A.Conceptualization, Methodology, Project administration, Supervision, Writing – review and editing |
| Authors: | Rimbi, P. T., O'Boyle, N., Douce, G. R., Pizza, M., Rosini, R., and Roe, A. J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Journal of Applied Microbiology |
| Publisher: | Oxford University Press |
| ISSN: | 1364-5072 |
| ISSN (Online): | 1365-2672 |
| Published Online: | 14 March 2024 |
| Copyright Holders: | Copyright © 2024 The Author(s) |
| First Published: | First published in Journal of Applied Microbiology 135(4): lxae067 |
| Publisher Policy: | Reproduced under a Creative Commons license |
University Staff: Request a correction | Enlighten Editors: Update this record
Funder and Project Information
The Role of Dietary D-serine in Health and Disease
Andrew Roe
BB/R006539/1
SII - Bacteriology
D-amino acids as modulatros of the genotoxic polyketide colibactin
Andrew Roe
BB/W015781/1
SII - Bacteriology
Aversion Therapy for Bacteria: Repurposing Aurodox
Andrew Roe
MR/V011499/1
Chemistry
Deposit and Record Details
| ID Code: | 322723 |
|---|---|
| Depositing User: | Ms Jacqui Brannan |
| Datestamp: | 14 Mar 2024 15:53 |
| Last Modified: | 11 Jun 2024 11:18 |
| Date of acceptance: | 13 March 2024 |
| Date of first online publication: | 14 March 2024 |
| Date Deposited: | 14 March 2024 |
| Data Availability Statement: | Yes |