The activities of suaveolol and other compounds from Hyptis suaveolens and Momordica charantia against the aetiological agents of African trypanosomiasis, leishmaniasis and malaria (original) (raw)
Oaikhena, E. E. et al. (2024) The activities of suaveolol and other compounds from Hyptis suaveolens and Momordica charantia against the aetiological agents of African trypanosomiasis, leishmaniasis and malaria.Experimental Parasitology, 263-64, 108807. (doi: 10.1016/j.exppara.2024.108807) (PMID:39043327)
Abstract
African trypanosomiasis and malaria are among the most severe health challenges to humans and livestock in Africa and new drugs are needed. Leaves of Hyptis suaveolens Kuntze (Lamiaceae) and Momordica charantia L. (Cucurbitaceae) were extracted with hexane, ethyl acetate, and then methanol, and subjected to silica gel column chromatography. Structures of six isolated compounds were elucidated through NMR and HR-EIMS spectrometry. Callistrisic acid, dehydroabietinol, suaveolic acid, suaveolol, and a mixture of suaveolol and suaveolic acid (SSA) were obtained from H. suaveolens, while karavilagenin D and momordicin I acetate were obtained from M. charantia. The isolated biomolecules were tested against trypomastigotes of Trypanosoma brucei brucei and T. congolense, and against Plasmodium falciparum. The most promising EC50 values were obtained for the purified suaveolol fraction, at 2.7 1± 0.36 μg/mL, and SSA, exhibiting an EC50 of 1.56 ± 0.17 μg/mL against T. b. brucei trypomastigotes. Suaveolic acid had low activity against T. b. brucei but displayed moderate activity against T. congolense trypomastigotes at 11.1 ± 0.5 μg/mL. Suaveolol and SSA were also tested against T. evansi, T. equiperdum, Leishmania major and L. mexicana but the antileishmanial activity was low. Neither of the active compounds, nor the mixture of the two, displayed any cytotoxic effect on human foreskin fibroblast (HFF) cells at even the highest concentration tested, being 200 μg/mL. We conclude that suaveolol and its mixture possessed significant and selective trypanocidal activity.
| Item Type: | Articles |
|---|---|
| Additional Information: | This study was supported by the Tertiary Education Trust Fund of the Federal Government of Nigeria as a form of studentship to O.E.E. M.A.U. was supported by a studentship from the Petroleum Technology Development Fund (Abuja, Nigeria) and a BBSRC Impact Accelerator Award to H.P.dK (BB/S506734/1), G.U.E. by a Royal Society Research Grant (RGS\R2\222269) and H.A.A.E. by a studentship from the Libyan government. H.P.dk and G.U.E. are members of the COST Action CA21111 (One Health drugs). |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Oaikhena, Miss Enimie and Quashie, Mr Neils and Ebiloma, Mr Godwin and Ungogo, Mr Marzuq and De Koning, Professor Harry and Elati, Hamza Ali Abd and Igoli, Professor John and Gray, Mr Alexander |
| Authors: | Oaikhena, E. E., Yahaya, U. A., Abdulsalami, S. M., Egbe, N. L., Adeyemi, M. M., Ungogo, M. A., Ebiloma, G. U., Zoiku, F. K., Fordjour, P. A., Elati, H. A.A., Quashie, N. B., Igoli, J. O., Gray, A. I., Lawson, C., Ferro, V. A., and de Koning, H. P. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Experimental Parasitology |
| Publisher: | Elsevier |
| ISSN: | 0014-4894 |
| ISSN (Online): | 1090-2449 |
| Published Online: | 21 July 2024 |
| Copyright Holders: | Copyright © 2024 The Authors |
| First Published: | First published in Experimental Parasitology 263-264: 108807 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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Funder and Project Information
BBSRC IAA application
Graeme Milligan
BB/S506734/1
School of Molecular Biosciences
Deposit and Record Details
| ID Code: | 330384 |
|---|---|
| Depositing User: | Mr Alastair Arthur |
| Datestamp: | 22 Jul 2024 13:47 |
| Last Modified: | 26 Oct 2024 11:56 |
| Date of acceptance: | 20 July 2024 |
| Date of first online publication: | 21 July 2024 |
| Date Deposited: | 22 July 2024 |
| Data Availability Statement: | Yes |