A novel Lawsonia intracellularis autotransporter protein is a prominent antigen (original) (raw)
Watson, E., Clark, E.M., Alberdi, M.P., Inglis, N.F., Porter, M., Imrie, L., Mclean, K., Manson, E., Lainson, A. and Smith, D.G.E.(2011) A novel Lawsonia intracellularis autotransporter protein is a prominent antigen.Clinical and Vaccine Immunology, 18(8), pp. 1282-1287. (doi: 10.1128/CVI.05073-11) (PMID:21697340) (PMCID:PMC3147349)
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Publisher's URL: http://dx.doi.org/10.1128/CVI.05073-11
Abstract
Investigation of antigenic determinants of the microaerophilic obligate intracellular bacterium Lawsonia intracellularis using a mass spectrometry approach identified a novel bacterial protein present in an extract of cell culture medium aspirated from heavily infected in vitro cell cultures. Western immunoblotting analysis of SDS-PAGE-resolved proteins using immune sera pooled from L. intracellularis-infected pigs revealed the presence of a strongly immunoreactive band of similar to 72 kDa. Liquid chromatography-electrospray ionization-tandem mass spectrometry analysis of this component and database mining using a fully annotated L. intracellularis genome sequence and the comprehensive GenBank prokaryotic genomic database highlighted the presence of a protein that shares little sequence similarity with other prokaryotic proteins and appears to be highly species specific. Detailed bioinformatic analyses identified the protein as member of the autotransporter protein family of surface-exposed proteins, and the designation LatA (Lawsonia autotransporter protein A) is suggested. Recognition of recombinant LatA on Western blots by a panel of sera from infected and control pigs corresponded 100% with a commercial serodiagnostic that relies on in vitro culture of this fastidious organism. LatA therefore represents a potential candidate for the development of a rapid and species-specific serodiagnostic reagent.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Smith, Professor David and Watson, Dr Eleanor and Alberdi, Dr Maria |
| Authors: | Watson, E., Clark, E.M., Alberdi, M.P., Inglis, N.F., Porter, M., Imrie, L., Mclean, K., Manson, E., Lainson, A., and Smith, D.G.E. |
| Subjects: | Q Science > QR Microbiology > QR180 Immunology |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Clinical and Vaccine Immunology |
| ISSN: | 1556-6811 |
| ISSN (Online): | 1556-679X |
| Published Online: | 22 June 2011 |
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Funder and Project Information
1
Molecular and functional characterisation of a type III secretion system (TTSS) of the obligate intracellular bacterium Lawsonia ....
David Smith
BB/C510532/1
Infection Immunity and Inflammation Life Sciences
Deposit and Record Details
| ID Code: | 55174 |
|---|---|
| Depositing User: | Mr Stuart Morrison |
| Datestamp: | 19 Oct 2011 10:46 |
| Last Modified: | 01 May 2025 17:49 |
| Date of first online publication: | 22 June 2011 |