Ihsan Keleş | Erciyes University (original) (raw)

Papers by Ihsan Keleş

Research paper thumbnail of Sığırların leptospirozisinden serum sialik asit ve lipid- bağlı sialik asit düzeyleri üzerine araştırma

Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi, 2000

Research paper thumbnail of Adenosine deaminase in the diagnosis of white muscle diseases in lambs

Medycyna Weterynaryjna, 2006

The aim of the study was to investigate the importance of ADA serum and its isoenzyme activity in... more The aim of the study was to investigate the importance of ADA serum and its isoenzyme activity in diagnosing white muscle disease in lambs. The animal material used in the study was forty seven Akkaraman lambs - twenty seven of which had clinical signs of white muscle disease (white muscle disease group) and twenty healthy lambs (control group). Blood samples were taken and Se levels, CK, LDH, AST, ADA and ADA izoenzyme activity were determined. Following this, the animals in the white muscle disease group received I mg sodium selenit + 60 mg vitamin E intramuscularly as a single dose. Fourteen days following sodyum selenit + vitamin E application blood samples were again taken and the same analysis repeated. Apart from ADA2 activity, other enzyme activities were significantly high (p < 0.001), and serum Se concentrations were significantly low (p < 0.001) in animals of the white muscle disease group compared to the control group. When the parameters obtained from the white muscle disease group were compared before and after treatment it was indicated that all enzyme parameters decreased significantly (p < 0.001) after treatment. On the other hand, serum Se concentration increased (p < 0.01) after treatment in the diseased group. The results confirm that determining ADA serum and its isoenzymes, together with CK, LDH and AST values seems to be useful in diagnosing white muscle disease

Research paper thumbnail of Effect of ozonated olive oil on experimentally induced skin infection by Streptococcus pyogenes and Staphylococcus aureus in rats

Antibiotic resistance problem is one of the most important problems in treating infectious diseas... more Antibiotic resistance problem is one of the most important problems in treating infectious diseases. To overcome such problems, agents apart from antibiotics that can be used in the treatment of infectious diseases and have no resistance problem should be explored. Natural substances, such as vegetable oils and ozone are possible resources. Thus, in the present study,we investigated the effect of ozonated olive oil on experimentally induced skin infection by Streptococcus pyogenes and Staphylococcus aureus in rats. The study was performed on 3 main groups. Experimental skin infection was induced by S. pyogenes (Group 1) or S. aureus (Group 2). Animals in Groups 1.1 (n=10) and 2.1 (n=10) received ozonated olive oil, in Groups 1.2 (n=10) and 2.2 (n=10) received olive oil, in Groups 1.3 (n=10) and 2.3 (n=10) received fucidic acid on the infection side in their skin at 24, 28, 32, 40, 48, 56, 64, 72, 80, 88, 96 hours. Groups 1.4 (n=11) and 2.4 (n=11) did not receive any treatment after infection. Group 3 (n=11) received only serum physiologic at above mentioned periods. After occurrence of the skin infection; ozonated olive oil had anti-microbial, anti-inflammatory effects and observed to have healing activity at 72-80 h after infection. Furthermore, the effects of ozonated olive oil were comparable with the effects of fusidic acid which its activity proved against skin infections with Staphylococcus and Streptococcus.

Research paper thumbnail of The efficacy of ricobendazole and ivermectin on naturally infected sheep with Trichostrongylidae sp. in the region of Van

Journal of Animal and Veterinary Advances, 2009

Abstract: The efficacy of two broad spectrum antelmentics (Ricobendazole and Ivermectin) against ... more Abstract: The efficacy of two broad spectrum antelmentics (Ricobendazole and Ivermectin) against gastrointestinal nematode infection was compared in sheep. Naturally infected thirty sheep with Trichostrongylidae sp., which were diagnosed by McMaster technique and ...

Research paper thumbnail of In-vitro studies on mechanisms of immunosuppression associated with bovine respiratory syncytial virus

Journal of Comparative Pathology, May 1, 1998

Bovine respiratory syncytial virus (BRSV) depressed the proliferative reactivity of normal ovine ... more Bovine respiratory syncytial virus (BRSV) depressed the proliferative reactivity of normal ovine peripheral blood lymphocytes to phytohaemagglutinin (PHA). This BRSV-induced reduction in proliferative reactivity was not reversed or ameliorated by the addition of (1) indomethacin or flunixin rneglurnine, substances known to inhibit the production of prostaglandins, or (2) the cytokines, interleukin-1 (IL-1) and interleukin-2 (IL-2), or (3) rat growth factor. The results suggest that the suppression of ovine lymphocyte reactivity to PHA associated with BRSV was not caused by the release of cyclooxygenase products such as prostaglandins, or the production of inhibitors of IL-1 or IL-2.

Research paper thumbnail of The effects of bovine respiratory syncytial virus on the phagocytic and antigen-presenting capacity of peripheral blood monocytes and monocytic cell lines derived from lambs and calves

Journal of Comparative Pathology, May 1, 1998

Human respiratory syncytial virus and bovine respiratory syncytial virus (BRSV) suppress lymphocy... more Human respiratory syncytial virus and bovine respiratory syncytial virus (BRSV) suppress lymphocyte response.s to mitogens, in the present study, the possible etfects of BRSV on some functions of antigen-presenting cells (APC) were investigated by exposing ovine monocytic cells to the virus before their use as APC. The depletion of monocytes from peripheral blood mononuclear cells resulted in the near total abrogation of proliferative responses to phytohaemagglutinin (PHA). Reactivity was restored by the addition of homologous monocytic cells derived from ovine peripheral blood monocytes as APC. The exposure of these monocytic cells to BRSV for 48h before their use as APC significantly reduced the proliferative responses of uninfected ovine lymphocytes to PHA. Furthermore, the exposure of bovine peripheral blood monocytcs and bovine and ovine monocytic cell lines to BRSV tbr 48h rcduced their capacity to phagocytize latex beads.

Research paper thumbnail of Seroprevalence of Q Fever in Cattle and Sheep in the East of Turkey

Asian Journal of Animal and Veterinary Advances, Apr 15, 2009

Research paper thumbnail of P. haemolytica A1′den Elde Edilen Lökotoksinin Bazı Hücresel Fonksiyonlar Üzerine Etkilerinin Araştırılması

Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi, Jun 1, 2001

Research paper thumbnail of Süksinilkolin'in Köpeklerde Elektrokardiyogram ve Kalp Atım Sayısı Üzerine Etkisi

DergiPark (Istanbul University), Mar 1, 2007

Süksinilkolinin 12 adet değişik yaş, cinsiyet ve ağırlıkta sağlıklı, melez köpekte elektrokardiyo... more Süksinilkolinin 12 adet değişik yaş, cinsiyet ve ağırlıkta sağlıklı, melez köpekte elektrokardiyogram ve kalp atım sayısı üzerine etkisi araştırıldı. 0.3 mg/kg dozunda kas içi olarak uygulanan süksinilkolin etkisiyle EKG'de: (a) Bradikardi. (b) Tam kalp bloğu. (c) S-T yükselmesi. (d) T dalgası'nda pik. (e) Q-T uzaması. (f) Kardiyak arrest. (g) QRS kompleksinin amplitüdünde artış görüldü. Bir köpekte de sinüs taşikardi belirlendi. Elektrokardiyogramdaki bu değişiklikler, elektrolitlerde özellikle de potasyum seviyesinde görülen artışın ve düzensizliğin miyokardiyal hücrelerdeki fizyolojik etkileriyle açıklanabilir.

Research paper thumbnail of The effects of virus-specific antibodies on the replication of bovine respiratory syncytial virus in vitro and on clinical disease and immune responses in lambs

Veterinary Immunology and Immunopathology, Apr 1, 1998

Low concentrations of antibodies, specific to human respiratory syncytial virus (RSV) have been s... more Low concentrations of antibodies, specific to human respiratory syncytial virus (RSV) have been shown to enhance virus replication in human monocytic cell lines by several workers. In the present study, replication of bovine RSV in ovine peripheral blood monocytes was shown to be enhanced in the presence of low concentration of bovine RSV-specific antibodies. Antibodies had no enhancing effect on virus replication in secondary lamb testis cells or monocytic cell lines derived from peripheral blood monocytes. The possible effects of low titres of bovine RSV-specific antibodies on the development of clinical disease were examined by inoculating groups of lambs with a mixture of virus and antibodies and assessing the severity of clinical disease and by measuring venous oxygen (PO2) and carbon dioxide (PCO2) tensions, as hypoxia has been associated with respiratory diseases. Inoculation of bovine RSV and virus-specific antibody complexes to lambs did not enhance clinical disease and had no effect on the clinical chemistry, haematology and PO2 and PCO2 tensions. Groups of lambs inoculated with virus alone or virus-antibody complexes developed significant humoral and cellular immune responses. There was no significant difference in the cellular immune responses of lambs exposed to virus alone and lambs exposed to virus-antibody mixture, as measured by virus-specific lymphocyte transformation or by cytotoxicity assays but the period of virus shedding was longer in lambs inoculated with a mixture of virus and immune serum.

Research paper thumbnail of Replication of bovine respiratory syncytial virus in bovine and ovine peripheral blood lymphocytes and monocytes and monocytic cell lines

Veterinary Microbiology, Apr 1, 1998

The present study compared the replication of bovine respiratory syncytial virus (BRSV) in bovine... more The present study compared the replication of bovine respiratory syncytial virus (BRSV) in bovine and ovine peripheral blood mononuclear cells, ovine and bovine monocytic cell lines and ovine alveolar macrophages. Low titres of virus were detected in ovine and bovine lymphocytes and monocytes 24±96 h post-exposure to the virus but there was no apparent replication of the virus in ovine alveolar macrophages during the culture period. The virus replicated to higher but statistically insignificant titres in ovine and bovine peripheral blood monocytes than in lymphocytes, with lymphocytes yielding peak titres significantly earlier. The secondary cell lines obtained from ovine liver and bone marrow also supported the replication of BRSV to high titres. The titres of BRSV in ovine and bovine lymphocytes and monocytes were significantly lower than in secondary cell lines. The addition of human recombinant tumour necrosis factor alpha after exposure to the virus or preincubation of ovine or bovine monocytic cells with either human recombinant interleukin 2 or phorbol myristate acetate before exposure to BRSV, did not significantly affect virus titre. Preincubation of cells with indomethacin or actinomycin significantly lowered virus titre (p<0.05).

Research paper thumbnail of The effects of bovine respiratory syncytial on normal ovine lymphocyte responses to mitogens or antigens in vitro

Comparative Immunology Microbiology and Infectious Diseases, 1999

In the present study peripheral blod mononuclear cells (MNC) obtained from normal uninfected lamb... more In the present study peripheral blod mononuclear cells (MNC) obtained from normal uninfected lambs were used to study the possible effects of bovine respiratory syncytial virus (BRSV) on lymphocyte responses to the mitogens, phytohaemagglutinin (PHA), concanavalin A (Con A) and pokeweed mitogen (PWM) in vitro. Live BRSV had a depressive effect on the proliferative responses of normal MNC to PHA, Con A and PWM. Inactivated BRSV and a commercial preparation of prostaglandin E2 were also found to depress the proliferative responses of normal ovine MNC to PHA but recombinant tumour necrosis factor-alpha (TNF-alpha) had no such effect. Serum samples obtained from BRSV-infected lambs contained substances inhibitory to PHA-driven lymphocyte blastogenesis. Memory blastogenic responses to border disease virus (BDV) of lymyphocytes obtained from lambs previously primed with BDV were significantly reduced when lymphocytes were exposed to infectious BRSV.

Research paper thumbnail of Vaccination with glutaraldehyde-fixed bovine respiratory syncytial virus (BRSV)-infected cells stimulates a better immune response in lambs than vaccination with heat-inactivated cell-free BRSV

Vaccine, Jul 1, 1998

The lamb model was used to investigate the possible protective efjects of vaccination with inacti... more The lamb model was used to investigate the possible protective efjects of vaccination with inactivated viral antigens against experimental infection with bovine respiratory syncytial virus. Two groups of eight lambs were vaccinated with either glutaraldehydeinactivated cell-associated virus or heat-inactivated cell-free virus and subsequently challenged with live virus, along with a group of naive lambs. The virus was shed for significantly longer periods, and the virus titres in nasal secretions were significantly higher in the group of naive lambs than in the two groups of vaccinated lambs. The period of virus-shedding in nasal secretions and virus titres was significantly lower (p ~0.01) in the group of lambs immunized with the cell-associated preparation. The same antigen stimulated better cellular immune responses as measured by virus-specific cytotoxicity or by virus-specific lymphocyte proliferation. However priming with inactivated vaccines had no significant effect on lymphocyte responses to phytohaemagglutinin, which was found to be significantly reduced (p < 0.01) following challenge with live virus.

Research paper thumbnail of Flor Zehirlenmesi ve Tedavisi

Research paper thumbnail of Atropine Sulphate Test Can Be an Aetiologic Indicator of Vagal Bradycardia Developed in a Megaoesophagus Case

Bulletin of The Veterinary Institute in Pulawy, 2007

An acquired megaoesophagus in a calf has been described. The diagnosis of megaoesophagus was made... more An acquired megaoesophagus in a calf has been described. The diagnosis of megaoesophagus was made based on clinical and laboratory examinations, using contrast radiography, and necropsy findings. Atropine sulphate was used to enlighten the relationship between damage in the N. vagus and bradycardia, which developed in the megaoesophagus case.

Research paper thumbnail of Serum Iron, Total Iron-Binding Capacity, Unbound Iron-Binding Capacity, Transferrin Saturation, Serum Copper, and Hematological Parameters in Pregnant Akkaraman Ewes Infected with Gastro-Intestinal Parasites

Turkish Journal of Veterinary & Animal Sciences, 2006

The aim of this study was to investigate serum iron parameters, and copper and iron deficiency an... more The aim of this study was to investigate serum iron parameters, and copper and iron deficiency anemia in ewes infected with gastro-intestinal parasites. This study was conducted with 60 pregnant Akkaraman ewes. In the statistical analyses, values obtained from both parasite infected and uninfected ewes were compared. Significant decreases were observed in PCV (P < 0.05), Hb (P < 0.05), RBC (P < 0.05), total protein (P < 0.05), albumin (P < 0.05), globulin (P < 0.05), and MCHC (P < 0.01), and a significant increase was seen in the WBC value (P < 0.05) in the parasite infected group. Furthermore, the parasite infected group had lower serum Fe (P < 0.001), TS % (P < 0.001), and serum Cu (P < 0.01) levels, and had higher UIBC values (P < 0.05) compared to the values obtain from the uninfected group. Therefore, in addition to antiparasite drug treatment in parasite infected pregnant ewes, iron preparations should also be administered in order to treat ...

Research paper thumbnail of Neonatal Buzaği İshallerinin İmmunokromotografik Test Kitleri İle Hizli Etiyolojik Teşhisi

SUMMARY In the present study, the most important etiologic agents of diarrhoea were diagnosed usi... more SUMMARY In the present study, the most important etiologic agents of diarrhoea were diagnosed using an immunochromatografic method. In this study, 51 calves with diarrhoea were used as material. Fecal samples were obtained from the calves with diarrhoea using rectal stimuli and etiological diagnosis was made using a quick immunochromatografic test kit. Furthermore, parasitological examination of the feces was made then treatment was applied according to diagnosis. One or more than one enteropathogen was diagnosed in 64.7% calves. The most common culprit were E. coli K99 (27.45%) and rotavirus (27.45%) infection(s) as alone or mix. Coronavirus determined only in one calf (1.96%). Mix etiology (3.92% E.coli K99 + Rotavirus, 1.96% E.coli K99 + E.coli CSA 31A, 1.96% Rotavirus + Cryptosporidium) was determined at 7.84%. In diarrhoeic calves, 11.76% of them had only parasiter enteropathogens (Eimeria 5.88%, Cryptosporidium 3.92%, Ascarid 1.96%). As a result; quick immunochromatografic tes...

Research paper thumbnail of Studies on the effects of x-ray on erythrocyte zinc and copper concentrations in rabbits after treatment with antioxidants

Biological trace element research, 2003

The aim of this study was to investigate the effect of supplemental antioxidant vitamins and mine... more The aim of this study was to investigate the effect of supplemental antioxidant vitamins and minerals on the erythrocyte concentrations of zinc and copper in rabbits after exposure to X-rays. The animals were divided into two experimental and one control group (CG). The first group (VG) was given daily oral doses of vitamins E and C; supplemental amounts of manganese, zinc, and copper were mixed with the feed and given to the second group of experimental animals (MG). Blood samples were taken from all groups before and after 4 wk of vitamin and mineral administration and after irradiation with a total dose of 550-rad X-rays. The administration of minerals caused the most significant increases of Zn and Cu. Even after irradiation, the zinc levels in the irradiated animals were higher than in the nonirradiated vitamin-supplemented animals (p<0.05). The results suggest that supplementation with antioxidant vitamins and minerals may have a protective effect against X-ray-induced damage.

Research paper thumbnail of Dermatitis solaris and its treatment in van cats

Indian Veterinary …, 2006

... Titre du document / Document title. Dermatitis solaris and its treatment in van cats. Auteur(... more ... Titre du document / Document title. Dermatitis solaris and its treatment in van cats. Auteur(s) / Author(s). KARACA Mehmet ; TUTUNCU Mehmet ; AKKAN Hasan Altan ; YUKSEL Hayati ; KELES Ihsan ; AGAOGLU Zahid ; Revue / Journal Title. ...

Research paper thumbnail of Serologic and microscopic studies on Babesiosis in horses in the Eastern border of Turkey

Journal of Equine Veterinary Science, 2003

W e wanted to determine the prevalence of Babesiosis in horses living in the Eastern border of Tu... more W e wanted to determine the prevalence of Babesiosis in horses living in the Eastern border of Turkey. One hundred ten horses were used. The incidence rate of Babesiosis was determined microscopically and by indirect fluorescence antibody (IFA) test. Microscopic examination of the blood smears revealed a 58.18% rate of infection with Babesia equi. On the other hand, IFA test results revealed 64.5% B equi and 4.5% B caballi infection rates, and a 0.9% infection with both. The different prevalence determined after microscopic examination and serologic test could be the result of latent progress of the disease, presence of pre-immunity, and possibility of missing some of the cases at microscopic examination. Overall, a high percentage of disease prevalence was observed compared with other studies performed in the different parts of Turkey. The reasons for this may be that the animals used in the current study were from the Eastern border of Turkey and are part of the illegal border trade with Iran, where prevalence of the disease in that country is not known. The other is that the owners of the horses used in the current study had not received information about how to combat tick infestation.

Research paper thumbnail of Sığırların leptospirozisinden serum sialik asit ve lipid- bağlı sialik asit düzeyleri üzerine araştırma

Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi, 2000

Research paper thumbnail of Adenosine deaminase in the diagnosis of white muscle diseases in lambs

Medycyna Weterynaryjna, 2006

The aim of the study was to investigate the importance of ADA serum and its isoenzyme activity in... more The aim of the study was to investigate the importance of ADA serum and its isoenzyme activity in diagnosing white muscle disease in lambs. The animal material used in the study was forty seven Akkaraman lambs - twenty seven of which had clinical signs of white muscle disease (white muscle disease group) and twenty healthy lambs (control group). Blood samples were taken and Se levels, CK, LDH, AST, ADA and ADA izoenzyme activity were determined. Following this, the animals in the white muscle disease group received I mg sodium selenit + 60 mg vitamin E intramuscularly as a single dose. Fourteen days following sodyum selenit + vitamin E application blood samples were again taken and the same analysis repeated. Apart from ADA2 activity, other enzyme activities were significantly high (p < 0.001), and serum Se concentrations were significantly low (p < 0.001) in animals of the white muscle disease group compared to the control group. When the parameters obtained from the white muscle disease group were compared before and after treatment it was indicated that all enzyme parameters decreased significantly (p < 0.001) after treatment. On the other hand, serum Se concentration increased (p < 0.01) after treatment in the diseased group. The results confirm that determining ADA serum and its isoenzymes, together with CK, LDH and AST values seems to be useful in diagnosing white muscle disease

Research paper thumbnail of Effect of ozonated olive oil on experimentally induced skin infection by Streptococcus pyogenes and Staphylococcus aureus in rats

Antibiotic resistance problem is one of the most important problems in treating infectious diseas... more Antibiotic resistance problem is one of the most important problems in treating infectious diseases. To overcome such problems, agents apart from antibiotics that can be used in the treatment of infectious diseases and have no resistance problem should be explored. Natural substances, such as vegetable oils and ozone are possible resources. Thus, in the present study,we investigated the effect of ozonated olive oil on experimentally induced skin infection by Streptococcus pyogenes and Staphylococcus aureus in rats. The study was performed on 3 main groups. Experimental skin infection was induced by S. pyogenes (Group 1) or S. aureus (Group 2). Animals in Groups 1.1 (n=10) and 2.1 (n=10) received ozonated olive oil, in Groups 1.2 (n=10) and 2.2 (n=10) received olive oil, in Groups 1.3 (n=10) and 2.3 (n=10) received fucidic acid on the infection side in their skin at 24, 28, 32, 40, 48, 56, 64, 72, 80, 88, 96 hours. Groups 1.4 (n=11) and 2.4 (n=11) did not receive any treatment after infection. Group 3 (n=11) received only serum physiologic at above mentioned periods. After occurrence of the skin infection; ozonated olive oil had anti-microbial, anti-inflammatory effects and observed to have healing activity at 72-80 h after infection. Furthermore, the effects of ozonated olive oil were comparable with the effects of fusidic acid which its activity proved against skin infections with Staphylococcus and Streptococcus.

Research paper thumbnail of The efficacy of ricobendazole and ivermectin on naturally infected sheep with Trichostrongylidae sp. in the region of Van

Journal of Animal and Veterinary Advances, 2009

Abstract: The efficacy of two broad spectrum antelmentics (Ricobendazole and Ivermectin) against ... more Abstract: The efficacy of two broad spectrum antelmentics (Ricobendazole and Ivermectin) against gastrointestinal nematode infection was compared in sheep. Naturally infected thirty sheep with Trichostrongylidae sp., which were diagnosed by McMaster technique and ...

Research paper thumbnail of In-vitro studies on mechanisms of immunosuppression associated with bovine respiratory syncytial virus

Journal of Comparative Pathology, May 1, 1998

Bovine respiratory syncytial virus (BRSV) depressed the proliferative reactivity of normal ovine ... more Bovine respiratory syncytial virus (BRSV) depressed the proliferative reactivity of normal ovine peripheral blood lymphocytes to phytohaemagglutinin (PHA). This BRSV-induced reduction in proliferative reactivity was not reversed or ameliorated by the addition of (1) indomethacin or flunixin rneglurnine, substances known to inhibit the production of prostaglandins, or (2) the cytokines, interleukin-1 (IL-1) and interleukin-2 (IL-2), or (3) rat growth factor. The results suggest that the suppression of ovine lymphocyte reactivity to PHA associated with BRSV was not caused by the release of cyclooxygenase products such as prostaglandins, or the production of inhibitors of IL-1 or IL-2.

Research paper thumbnail of The effects of bovine respiratory syncytial virus on the phagocytic and antigen-presenting capacity of peripheral blood monocytes and monocytic cell lines derived from lambs and calves

Journal of Comparative Pathology, May 1, 1998

Human respiratory syncytial virus and bovine respiratory syncytial virus (BRSV) suppress lymphocy... more Human respiratory syncytial virus and bovine respiratory syncytial virus (BRSV) suppress lymphocyte response.s to mitogens, in the present study, the possible etfects of BRSV on some functions of antigen-presenting cells (APC) were investigated by exposing ovine monocytic cells to the virus before their use as APC. The depletion of monocytes from peripheral blood mononuclear cells resulted in the near total abrogation of proliferative responses to phytohaemagglutinin (PHA). Reactivity was restored by the addition of homologous monocytic cells derived from ovine peripheral blood monocytes as APC. The exposure of these monocytic cells to BRSV for 48h before their use as APC significantly reduced the proliferative responses of uninfected ovine lymphocytes to PHA. Furthermore, the exposure of bovine peripheral blood monocytcs and bovine and ovine monocytic cell lines to BRSV tbr 48h rcduced their capacity to phagocytize latex beads.

Research paper thumbnail of Seroprevalence of Q Fever in Cattle and Sheep in the East of Turkey

Asian Journal of Animal and Veterinary Advances, Apr 15, 2009

Research paper thumbnail of P. haemolytica A1′den Elde Edilen Lökotoksinin Bazı Hücresel Fonksiyonlar Üzerine Etkilerinin Araştırılması

Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi, Jun 1, 2001

Research paper thumbnail of Süksinilkolin'in Köpeklerde Elektrokardiyogram ve Kalp Atım Sayısı Üzerine Etkisi

DergiPark (Istanbul University), Mar 1, 2007

Süksinilkolinin 12 adet değişik yaş, cinsiyet ve ağırlıkta sağlıklı, melez köpekte elektrokardiyo... more Süksinilkolinin 12 adet değişik yaş, cinsiyet ve ağırlıkta sağlıklı, melez köpekte elektrokardiyogram ve kalp atım sayısı üzerine etkisi araştırıldı. 0.3 mg/kg dozunda kas içi olarak uygulanan süksinilkolin etkisiyle EKG'de: (a) Bradikardi. (b) Tam kalp bloğu. (c) S-T yükselmesi. (d) T dalgası'nda pik. (e) Q-T uzaması. (f) Kardiyak arrest. (g) QRS kompleksinin amplitüdünde artış görüldü. Bir köpekte de sinüs taşikardi belirlendi. Elektrokardiyogramdaki bu değişiklikler, elektrolitlerde özellikle de potasyum seviyesinde görülen artışın ve düzensizliğin miyokardiyal hücrelerdeki fizyolojik etkileriyle açıklanabilir.

Research paper thumbnail of The effects of virus-specific antibodies on the replication of bovine respiratory syncytial virus in vitro and on clinical disease and immune responses in lambs

Veterinary Immunology and Immunopathology, Apr 1, 1998

Low concentrations of antibodies, specific to human respiratory syncytial virus (RSV) have been s... more Low concentrations of antibodies, specific to human respiratory syncytial virus (RSV) have been shown to enhance virus replication in human monocytic cell lines by several workers. In the present study, replication of bovine RSV in ovine peripheral blood monocytes was shown to be enhanced in the presence of low concentration of bovine RSV-specific antibodies. Antibodies had no enhancing effect on virus replication in secondary lamb testis cells or monocytic cell lines derived from peripheral blood monocytes. The possible effects of low titres of bovine RSV-specific antibodies on the development of clinical disease were examined by inoculating groups of lambs with a mixture of virus and antibodies and assessing the severity of clinical disease and by measuring venous oxygen (PO2) and carbon dioxide (PCO2) tensions, as hypoxia has been associated with respiratory diseases. Inoculation of bovine RSV and virus-specific antibody complexes to lambs did not enhance clinical disease and had no effect on the clinical chemistry, haematology and PO2 and PCO2 tensions. Groups of lambs inoculated with virus alone or virus-antibody complexes developed significant humoral and cellular immune responses. There was no significant difference in the cellular immune responses of lambs exposed to virus alone and lambs exposed to virus-antibody mixture, as measured by virus-specific lymphocyte transformation or by cytotoxicity assays but the period of virus shedding was longer in lambs inoculated with a mixture of virus and immune serum.

Research paper thumbnail of Replication of bovine respiratory syncytial virus in bovine and ovine peripheral blood lymphocytes and monocytes and monocytic cell lines

Veterinary Microbiology, Apr 1, 1998

The present study compared the replication of bovine respiratory syncytial virus (BRSV) in bovine... more The present study compared the replication of bovine respiratory syncytial virus (BRSV) in bovine and ovine peripheral blood mononuclear cells, ovine and bovine monocytic cell lines and ovine alveolar macrophages. Low titres of virus were detected in ovine and bovine lymphocytes and monocytes 24±96 h post-exposure to the virus but there was no apparent replication of the virus in ovine alveolar macrophages during the culture period. The virus replicated to higher but statistically insignificant titres in ovine and bovine peripheral blood monocytes than in lymphocytes, with lymphocytes yielding peak titres significantly earlier. The secondary cell lines obtained from ovine liver and bone marrow also supported the replication of BRSV to high titres. The titres of BRSV in ovine and bovine lymphocytes and monocytes were significantly lower than in secondary cell lines. The addition of human recombinant tumour necrosis factor alpha after exposure to the virus or preincubation of ovine or bovine monocytic cells with either human recombinant interleukin 2 or phorbol myristate acetate before exposure to BRSV, did not significantly affect virus titre. Preincubation of cells with indomethacin or actinomycin significantly lowered virus titre (p<0.05).

Research paper thumbnail of The effects of bovine respiratory syncytial on normal ovine lymphocyte responses to mitogens or antigens in vitro

Comparative Immunology Microbiology and Infectious Diseases, 1999

In the present study peripheral blod mononuclear cells (MNC) obtained from normal uninfected lamb... more In the present study peripheral blod mononuclear cells (MNC) obtained from normal uninfected lambs were used to study the possible effects of bovine respiratory syncytial virus (BRSV) on lymphocyte responses to the mitogens, phytohaemagglutinin (PHA), concanavalin A (Con A) and pokeweed mitogen (PWM) in vitro. Live BRSV had a depressive effect on the proliferative responses of normal MNC to PHA, Con A and PWM. Inactivated BRSV and a commercial preparation of prostaglandin E2 were also found to depress the proliferative responses of normal ovine MNC to PHA but recombinant tumour necrosis factor-alpha (TNF-alpha) had no such effect. Serum samples obtained from BRSV-infected lambs contained substances inhibitory to PHA-driven lymphocyte blastogenesis. Memory blastogenic responses to border disease virus (BDV) of lymyphocytes obtained from lambs previously primed with BDV were significantly reduced when lymphocytes were exposed to infectious BRSV.

Research paper thumbnail of Vaccination with glutaraldehyde-fixed bovine respiratory syncytial virus (BRSV)-infected cells stimulates a better immune response in lambs than vaccination with heat-inactivated cell-free BRSV

Vaccine, Jul 1, 1998

The lamb model was used to investigate the possible protective efjects of vaccination with inacti... more The lamb model was used to investigate the possible protective efjects of vaccination with inactivated viral antigens against experimental infection with bovine respiratory syncytial virus. Two groups of eight lambs were vaccinated with either glutaraldehydeinactivated cell-associated virus or heat-inactivated cell-free virus and subsequently challenged with live virus, along with a group of naive lambs. The virus was shed for significantly longer periods, and the virus titres in nasal secretions were significantly higher in the group of naive lambs than in the two groups of vaccinated lambs. The period of virus-shedding in nasal secretions and virus titres was significantly lower (p ~0.01) in the group of lambs immunized with the cell-associated preparation. The same antigen stimulated better cellular immune responses as measured by virus-specific cytotoxicity or by virus-specific lymphocyte proliferation. However priming with inactivated vaccines had no significant effect on lymphocyte responses to phytohaemagglutinin, which was found to be significantly reduced (p < 0.01) following challenge with live virus.

Research paper thumbnail of Flor Zehirlenmesi ve Tedavisi

Research paper thumbnail of Atropine Sulphate Test Can Be an Aetiologic Indicator of Vagal Bradycardia Developed in a Megaoesophagus Case

Bulletin of The Veterinary Institute in Pulawy, 2007

An acquired megaoesophagus in a calf has been described. The diagnosis of megaoesophagus was made... more An acquired megaoesophagus in a calf has been described. The diagnosis of megaoesophagus was made based on clinical and laboratory examinations, using contrast radiography, and necropsy findings. Atropine sulphate was used to enlighten the relationship between damage in the N. vagus and bradycardia, which developed in the megaoesophagus case.

Research paper thumbnail of Serum Iron, Total Iron-Binding Capacity, Unbound Iron-Binding Capacity, Transferrin Saturation, Serum Copper, and Hematological Parameters in Pregnant Akkaraman Ewes Infected with Gastro-Intestinal Parasites

Turkish Journal of Veterinary & Animal Sciences, 2006

The aim of this study was to investigate serum iron parameters, and copper and iron deficiency an... more The aim of this study was to investigate serum iron parameters, and copper and iron deficiency anemia in ewes infected with gastro-intestinal parasites. This study was conducted with 60 pregnant Akkaraman ewes. In the statistical analyses, values obtained from both parasite infected and uninfected ewes were compared. Significant decreases were observed in PCV (P < 0.05), Hb (P < 0.05), RBC (P < 0.05), total protein (P < 0.05), albumin (P < 0.05), globulin (P < 0.05), and MCHC (P < 0.01), and a significant increase was seen in the WBC value (P < 0.05) in the parasite infected group. Furthermore, the parasite infected group had lower serum Fe (P < 0.001), TS % (P < 0.001), and serum Cu (P < 0.01) levels, and had higher UIBC values (P < 0.05) compared to the values obtain from the uninfected group. Therefore, in addition to antiparasite drug treatment in parasite infected pregnant ewes, iron preparations should also be administered in order to treat ...

Research paper thumbnail of Neonatal Buzaği İshallerinin İmmunokromotografik Test Kitleri İle Hizli Etiyolojik Teşhisi

SUMMARY In the present study, the most important etiologic agents of diarrhoea were diagnosed usi... more SUMMARY In the present study, the most important etiologic agents of diarrhoea were diagnosed using an immunochromatografic method. In this study, 51 calves with diarrhoea were used as material. Fecal samples were obtained from the calves with diarrhoea using rectal stimuli and etiological diagnosis was made using a quick immunochromatografic test kit. Furthermore, parasitological examination of the feces was made then treatment was applied according to diagnosis. One or more than one enteropathogen was diagnosed in 64.7% calves. The most common culprit were E. coli K99 (27.45%) and rotavirus (27.45%) infection(s) as alone or mix. Coronavirus determined only in one calf (1.96%). Mix etiology (3.92% E.coli K99 + Rotavirus, 1.96% E.coli K99 + E.coli CSA 31A, 1.96% Rotavirus + Cryptosporidium) was determined at 7.84%. In diarrhoeic calves, 11.76% of them had only parasiter enteropathogens (Eimeria 5.88%, Cryptosporidium 3.92%, Ascarid 1.96%). As a result; quick immunochromatografic tes...

Research paper thumbnail of Studies on the effects of x-ray on erythrocyte zinc and copper concentrations in rabbits after treatment with antioxidants

Biological trace element research, 2003

The aim of this study was to investigate the effect of supplemental antioxidant vitamins and mine... more The aim of this study was to investigate the effect of supplemental antioxidant vitamins and minerals on the erythrocyte concentrations of zinc and copper in rabbits after exposure to X-rays. The animals were divided into two experimental and one control group (CG). The first group (VG) was given daily oral doses of vitamins E and C; supplemental amounts of manganese, zinc, and copper were mixed with the feed and given to the second group of experimental animals (MG). Blood samples were taken from all groups before and after 4 wk of vitamin and mineral administration and after irradiation with a total dose of 550-rad X-rays. The administration of minerals caused the most significant increases of Zn and Cu. Even after irradiation, the zinc levels in the irradiated animals were higher than in the nonirradiated vitamin-supplemented animals (p<0.05). The results suggest that supplementation with antioxidant vitamins and minerals may have a protective effect against X-ray-induced damage.

Research paper thumbnail of Dermatitis solaris and its treatment in van cats

Indian Veterinary …, 2006

... Titre du document / Document title. Dermatitis solaris and its treatment in van cats. Auteur(... more ... Titre du document / Document title. Dermatitis solaris and its treatment in van cats. Auteur(s) / Author(s). KARACA Mehmet ; TUTUNCU Mehmet ; AKKAN Hasan Altan ; YUKSEL Hayati ; KELES Ihsan ; AGAOGLU Zahid ; Revue / Journal Title. ...

Research paper thumbnail of Serologic and microscopic studies on Babesiosis in horses in the Eastern border of Turkey

Journal of Equine Veterinary Science, 2003

W e wanted to determine the prevalence of Babesiosis in horses living in the Eastern border of Tu... more W e wanted to determine the prevalence of Babesiosis in horses living in the Eastern border of Turkey. One hundred ten horses were used. The incidence rate of Babesiosis was determined microscopically and by indirect fluorescence antibody (IFA) test. Microscopic examination of the blood smears revealed a 58.18% rate of infection with Babesia equi. On the other hand, IFA test results revealed 64.5% B equi and 4.5% B caballi infection rates, and a 0.9% infection with both. The different prevalence determined after microscopic examination and serologic test could be the result of latent progress of the disease, presence of pre-immunity, and possibility of missing some of the cases at microscopic examination. Overall, a high percentage of disease prevalence was observed compared with other studies performed in the different parts of Turkey. The reasons for this may be that the animals used in the current study were from the Eastern border of Turkey and are part of the illegal border trade with Iran, where prevalence of the disease in that country is not known. The other is that the owners of the horses used in the current study had not received information about how to combat tick infestation.