Neptali Gutierrez - ESCUELA DE INGENIEROS MILITARES (original) (raw)

Maziar Divangahi related author profile picture

Jim Sun related author profile picture

Heinz Remold related author profile picture

Wolfram Brune related author profile picture

Tom Vanden Berghe related author profile picture

Yadira Simón Palacios related author profile picture

Luis Torre-Bouscoulet related author profile picture

Joanne Hildebrand related author profile picture

Malgorzata Krzyzowska related author profile picture

Susanne Kirschnek related author profile picture

Uploads

Papers by Neptali Gutierrez

Research paper thumbnail of Diverse Cell Death Mechanisms Are Simultaneously Activated in Macrophages Infected by Virulent Mycobacterium tuberculosis

Pathogens

Macrophages are necessary to eliminate pathogens. However, some pathogens have developed mechanis... more Macrophages are necessary to eliminate pathogens. However, some pathogens have developed mechanisms to avoid the immune response. One of them is modulating the cell death mechanism to favor pathogen survival. In this study, we evaluated if virulent Mycobacterium tuberculosis (M. tb) can simultaneously activate more than one cell death mechanism. We infected human monocyte-derived macrophages (MDM) in vitro with avirulent (H37Ra) and virulent (H37Rv) strains, and then we measured molecules involved in apoptosis, necroptosis, and pyroptosis. Our data showed that H37Rv infection increased the BCL-2 transcript and protein, decreased the BAX transcript, and increased phosphorylated BCL-2 at the protein level. Moreover, H37Rv infection increased the expression of the molecules involved in the necroptotic pathway, such as ASK1, p-38, RIPK1, RIPK3, and caspase-8, while H37Ra increased caspase-8 and decreased RIPK3 at the transcriptional level. In addition, NLRP3 and CASP1 expression was inc...

Research paper thumbnail of Cell Cycle Inhibitors in Cancer Therapy

Research paper thumbnail of PCR EN TIEMPO REAL

Research paper thumbnail of Guia ElabCitas y Ref Estilo Vancouver

Research paper thumbnail of CÓMO FUNCIONA UNA CÉLULA? FISIOLOGÍA CELULAR

FISIOLOGIA

FISILOGIA CELULAR

Research paper thumbnail of ENFERMEDAD-EPIGENETICA

Research paper thumbnail of Diverse Cell Death Mechanisms Are Simultaneously Activated in Macrophages Infected by Virulent Mycobacterium tuberculosis

Pathogens

Macrophages are necessary to eliminate pathogens. However, some pathogens have developed mechanis... more Macrophages are necessary to eliminate pathogens. However, some pathogens have developed mechanisms to avoid the immune response. One of them is modulating the cell death mechanism to favor pathogen survival. In this study, we evaluated if virulent Mycobacterium tuberculosis (M. tb) can simultaneously activate more than one cell death mechanism. We infected human monocyte-derived macrophages (MDM) in vitro with avirulent (H37Ra) and virulent (H37Rv) strains, and then we measured molecules involved in apoptosis, necroptosis, and pyroptosis. Our data showed that H37Rv infection increased the BCL-2 transcript and protein, decreased the BAX transcript, and increased phosphorylated BCL-2 at the protein level. Moreover, H37Rv infection increased the expression of the molecules involved in the necroptotic pathway, such as ASK1, p-38, RIPK1, RIPK3, and caspase-8, while H37Ra increased caspase-8 and decreased RIPK3 at the transcriptional level. In addition, NLRP3 and CASP1 expression was inc...

Research paper thumbnail of Cell Cycle Inhibitors in Cancer Therapy

Research paper thumbnail of PCR EN TIEMPO REAL

Research paper thumbnail of Guia ElabCitas y Ref Estilo Vancouver

Research paper thumbnail of CÓMO FUNCIONA UNA CÉLULA? FISIOLOGÍA CELULAR

FISIOLOGIA

FISILOGIA CELULAR

Research paper thumbnail of ENFERMEDAD-EPIGENETICA

Log In