Michael Black | ESPOL (Escuela Superior Politécnica del Litoral) (original) (raw)
Papers by Michael Black
PloS one, 2014
Adipose stem cell (ASC) differentiation is necessary for the proper maintenance and function of a... more Adipose stem cell (ASC) differentiation is necessary for the proper maintenance and function of adipose tissue. The procurement and characterization of multipotent ASCs has enabled investigation into the molecular determinants driving human adipogenesis. Here, the transcription factor MYC was identified as a significant regulator of ASC differentiation. Expression of MYC transcript and protein was found to accumulate during the initial course of differentiation. Loss-of-function analysis using siRNA mediated knockdown of MYC demonstrated inhibition of hormonally stimulated adipogenesis. MYC exhibited an early and sustained expression pattern that preceded down regulation of key suppressor genes, as well as induction of transcriptional and functional effectors. Glucocorticoid stimulation was identified as a necessary component for MYC induction and was found to impact adipogenesis in a concentration-dependent manner. Global gene expression analysis of MYC knockdown in ASC enriched fo...
Toxicological sciences : an official journal of the Society of Toxicology, 2014
Relative to microarrays, RNA-seq has been reported to offer higher precision estimates of transcr... more Relative to microarrays, RNA-seq has been reported to offer higher precision estimates of transcript abundance, a greater dynamic range, and detection of novel transcripts. However, previous comparisons of the 2 technologies have not covered dose-response experiments that are relevant to toxicology. Male F344 rats were exposed for 13 weeks to 5 doses of bromobenzene, and liver gene expression was measured using both microarrays and RNA-seq. Multiple normalization methods were evaluated for each technology, and gene expression changes were statistically analyzed using both analysis of variance and benchmark dose (BMD). Fold-change values were highly correlated between the 2 technologies, whereas the -log p values showed lower correlation. RNA-seq detected fewer statistically significant genes at lower doses, but more significant genes based on fold change except when a negative binomial transformation was applied. Overlap in genes significant by both p value and fold change was appro...
Deep Sea Research Part II: Topical Studies in Oceanography, 1998
Lecture Notes in Computer Science, 2014
2011 5th International IEEE/EMBS Conference on Neural Engineering, 2011
IEEE Transactions on Neural Systems and Rehabilitation Engineering, 2000
Annals of Surgical Oncology, 2007
Lecture Notes in Computer Science, 1996
Lecture Notes in Computer Science, 2010
Proceedings of the Second International Conference on Automatic Face and Gesture Recognition, 1996
2006 International Conference of the IEEE Engineering in Medicine and Biology Society, 2006
International Journal of Computer Vision, 2010
International Journal of Computer Vision, 1996
Image and Vision Computing, 1998
This paper overviews the PLAYBOT project, a long-term, large-scale research program whose goal is... more This paper overviews the PLAYBOT project, a long-term, large-scale research program whose goal is to provide a directable robot which may enable physically disabled children to access and manipulate toys. This domain is the first test domain, but there is nothing inherent in the design of PLAYBOT that prohibits its extension to other tasks. The research is guided by several important goals: vision is the primary sensor; vision is task directed; the robot must be able to visually search its environment; object and event recognition are ...
IEEE Transactions on Image Processing, 1998
Relations between anisotropic diffusion and robust statistics are described in this paper. Specif... more Relations between anisotropic diffusion and robust statistics are described in this paper. Specifically, we show that anisotropic diffusion can be seen as a robust estimation procedure that estimates a piecewise smooth image from a noisy input image. The "edge-stopping" function in the anisotropic diffusion equation is closely related to the error norm and influence function in the robust estimation framework. This connection leads to a new "edge-stopping" function based on Tukey's biweight robust estimator that preserves sharper boundaries than previous formulations and improves the automatic stopping of the diffusion. The robust statistical interpretation also provides a means for detecting the boundaries (edges) between the piecewise smooth regions in an image that has been smoothed with anisotropic diffusion. Additionally, we derive a relationship between anisotropic diffusion and regularization with line processes. Adding constraints on the spatial organization of the line processes allows us to develop new anisotropic diffusion equations that result in a qualitative improvement in the continuity of edges.
Computer Vision and Image Understanding, 1999
Computer Vision and Image Understanding, 1996
PloS one, 2014
Adipose stem cell (ASC) differentiation is necessary for the proper maintenance and function of a... more Adipose stem cell (ASC) differentiation is necessary for the proper maintenance and function of adipose tissue. The procurement and characterization of multipotent ASCs has enabled investigation into the molecular determinants driving human adipogenesis. Here, the transcription factor MYC was identified as a significant regulator of ASC differentiation. Expression of MYC transcript and protein was found to accumulate during the initial course of differentiation. Loss-of-function analysis using siRNA mediated knockdown of MYC demonstrated inhibition of hormonally stimulated adipogenesis. MYC exhibited an early and sustained expression pattern that preceded down regulation of key suppressor genes, as well as induction of transcriptional and functional effectors. Glucocorticoid stimulation was identified as a necessary component for MYC induction and was found to impact adipogenesis in a concentration-dependent manner. Global gene expression analysis of MYC knockdown in ASC enriched fo...
Toxicological sciences : an official journal of the Society of Toxicology, 2014
Relative to microarrays, RNA-seq has been reported to offer higher precision estimates of transcr... more Relative to microarrays, RNA-seq has been reported to offer higher precision estimates of transcript abundance, a greater dynamic range, and detection of novel transcripts. However, previous comparisons of the 2 technologies have not covered dose-response experiments that are relevant to toxicology. Male F344 rats were exposed for 13 weeks to 5 doses of bromobenzene, and liver gene expression was measured using both microarrays and RNA-seq. Multiple normalization methods were evaluated for each technology, and gene expression changes were statistically analyzed using both analysis of variance and benchmark dose (BMD). Fold-change values were highly correlated between the 2 technologies, whereas the -log p values showed lower correlation. RNA-seq detected fewer statistically significant genes at lower doses, but more significant genes based on fold change except when a negative binomial transformation was applied. Overlap in genes significant by both p value and fold change was appro...
Deep Sea Research Part II: Topical Studies in Oceanography, 1998
Lecture Notes in Computer Science, 2014
2011 5th International IEEE/EMBS Conference on Neural Engineering, 2011
IEEE Transactions on Neural Systems and Rehabilitation Engineering, 2000
Annals of Surgical Oncology, 2007
Lecture Notes in Computer Science, 1996
Lecture Notes in Computer Science, 2010
Proceedings of the Second International Conference on Automatic Face and Gesture Recognition, 1996
2006 International Conference of the IEEE Engineering in Medicine and Biology Society, 2006
International Journal of Computer Vision, 2010
International Journal of Computer Vision, 1996
Image and Vision Computing, 1998
This paper overviews the PLAYBOT project, a long-term, large-scale research program whose goal is... more This paper overviews the PLAYBOT project, a long-term, large-scale research program whose goal is to provide a directable robot which may enable physically disabled children to access and manipulate toys. This domain is the first test domain, but there is nothing inherent in the design of PLAYBOT that prohibits its extension to other tasks. The research is guided by several important goals: vision is the primary sensor; vision is task directed; the robot must be able to visually search its environment; object and event recognition are ...
IEEE Transactions on Image Processing, 1998
Relations between anisotropic diffusion and robust statistics are described in this paper. Specif... more Relations between anisotropic diffusion and robust statistics are described in this paper. Specifically, we show that anisotropic diffusion can be seen as a robust estimation procedure that estimates a piecewise smooth image from a noisy input image. The "edge-stopping" function in the anisotropic diffusion equation is closely related to the error norm and influence function in the robust estimation framework. This connection leads to a new "edge-stopping" function based on Tukey's biweight robust estimator that preserves sharper boundaries than previous formulations and improves the automatic stopping of the diffusion. The robust statistical interpretation also provides a means for detecting the boundaries (edges) between the piecewise smooth regions in an image that has been smoothed with anisotropic diffusion. Additionally, we derive a relationship between anisotropic diffusion and regularization with line processes. Adding constraints on the spatial organization of the line processes allows us to develop new anisotropic diffusion equations that result in a qualitative improvement in the continuity of edges.
Computer Vision and Image Understanding, 1999
Computer Vision and Image Understanding, 1996