Marcelo Santos | Universidade Estacio de Sa, Brasil (original) (raw)
Papers by Marcelo Santos
Revista de Sociologia e Política, 2009
Redalyc. Red de Revistas Científicas de América Latina y el Caribe, España y Portugal Sistema de ... more Redalyc. Red de Revistas Científicas de América Latina y el Caribe, España y Portugal Sistema de Información Científica Redalyc Hemeroteca. ...
The Protein Journal, 2011
Plant Signaling & Behavior, 2009
Revista Brasileira de Farmacognosia
Myrciaria floribunda (H. West ex Willd.) O. Berg, Myrtaceae, is popularly known as “camboim-amare... more Myrciaria floribunda (H. West ex Willd.) O. Berg, Myrtaceae, is popularly known as “camboim-amarelo” and was collected at Restinga de Jurubatiba (RJ, Brazil). Leaves from this species were submitted to hydrodistillation to extract its essential oil. Monoterpenes were the main compounds found (53.9%), and 1,8-cineole was the major constituent (38.4%). Studies were carried out to evaluate the effects of this essential oil on the development of two species of agricultural pests (Oncopeltus fasciatus and Dysdercus peruvianus). The essential oil was considered effective against D. peruvianus and O. fasciatus, causing mortality in both insects. The LD50 values (μg/insect) observed were 112.44 μg/insect (O. fasciatus) and 309.64 μg/insect (D. peruvianus) after one day of treatment, and 72.18 μg/insect (O. fasciatus) and 94.42 μg/insect (D. peruvianus) after 22 days of treatment. The present study reports for the first time the bioinsecticidal activity of essential oil of Myrciaria floribun...
Revista Brasileira de Farmacognosia
Essential oils are known for their insect control potential, which is mainly attributed to the pr... more Essential oils are known for their insect control potential, which is mainly attributed to the presence of terpenes that interfere with hormonal and physiological processes of arthropods. The aim of the present study was to evaluate the effects of essential oil from the leaves of Eugenia sulcata Spring ex Mart., Myrtaceae, on the development of two species of agricultural pest insects, Dysdercus peruvianus and Oncopeltus fasciatus. Results showed that the essential oil induced mortality, and reduced numbers of adults. Topical treatment of Oncopeltus fasciatus using pure essential oil caused significant mortality rates (96.67%), while Dysdercus peruvianus had a higher tolerance, with 80% mortality at the end of the experiments. Results suggest that essential oil from the leaves of Eugenia sulcata may be used in agriculture for insect pest control.
Memórias do Instituto Oswaldo Cruz, 2015
Peptides, 2011
Antifungal proteins and peptides, essential compounds for plant defense, have been isolated from ... more Antifungal proteins and peptides, essential compounds for plant defense, have been isolated from several tissues of various plants. These proteins could be used as a natural alternative to control phytopathogenic fungi. In this report a heterodimeric antifungal protein named Pa-AFP1, showing higher identity with the 2S albumin family, was purified by using 70-100% ammonium sulfate saturation and further purification steps such as anionic exchange Q-Sepharose chromatography associated with HPLC reversed-phase C4 chromatography. Analysis by Tricine-SDS-PAGE revealed two peptidic molecular masses of approximately 4500 Da and 7000 Da, in the presence of β-mercaptoethanol, while by removing the reducing agent a single protein with molecular mass of about 11,500 Da was obtained. Moreover, dimer mass was confirmed by MALDI-TOF analyses (11,569.76 Da). The antifungal protein, named Pa-AFP1, efficiently inhibited the growth of filamentous fungi Colletotrichum gloeosporioides, and was added to a short list of 2S albumins with antimicrobial properties. Otherwise, this same peptide showed no activity toward bacteria and yeasts. In summary, this compound could be used in the future to develop biotechnological products for the control of phytopathogenic fungi.
Neomitranthes obscura (DC.) N. Silveira is an endemic plant of Brazilian Atlantic Forest and wide... more Neomitranthes obscura (DC.) N. Silveira is an endemic plant of Brazilian Atlantic Forest and widely spread in the sandbanks of "Restinga de Jurubatiba" National Park. It is popularly known by local population as "camboim-de-cachorro" or "cambuí-preto" and recognized by its black ripe fruits. However, specimens with yellow ripe fruits were localized in the "Restinga de Jurubatiba" National Park. The aim of the present study was to evaluate chemical composition of essential oils obtained from leaves and fruits of N. obscura specimens with different fruit color (black and yellow) by GC and GC-MS. Essential oils from leaves of specimens with black and yellow fruits indicated a predominance of sesquiterpenes (81.1% and 84.8%, resp.). Meanwhile, essential oil from black fruits presented a predominance of monoterpenes (50.5%), while essential oil from yellow fruits had sesquiterpenes (39.9%) as major substances. Despite previous studies about this sp...
In Vitro Cellular & Developmental Biology - Plant, 2006
LATIN AMERICAN JOURNAL OF PHARMACY
Manilkara subsericea (Mart.) Dubard (Sapotaceae) is popularly known in Brazil as “guracica”. The ... more Manilkara subsericea (Mart.) Dubard (Sapotaceae) is popularly known in Brazil as “guracica”. The species from the genus Manilkara have been studied and several substances were found, including triterpenes. In the present study, we describe the anticholinesterasic activity of an extract of M. subsericea containg beta-amyrin acetate (76.3 %) and alpha-amyrin acetate (23.7 %) using a TLC bioassay
Developmental & Comparative Immunology, 2011
LATIN AMERICAN JOURNAL OF PHARMACY
The chemical composition of the essential oils from leaves and stems of Eugenia sulcata Spring ex... more The chemical composition of the essential oils from leaves and stems of Eugenia sulcata Spring ex Mart., obtained by hydrodistillation, was analyzed by GC-MS and quantified by CG-FID. In all, 37 components were identified and sesquiterpenes represented the largest fraction of both oils, in the leaves (58.2 %) and stems (85.3 %). The major constituent found in the essential oil from leaves and stems of E. sulcata was β-caryophyllene, corresponding to 24.6 % and 18.8 %, respectively. The substances αcubebene (1.1 %), β-copaene (0.5 %), cis-muurola-3,5-diene (0.6 %), cis-muurola-4(14),5-diene (1.3 %), γhimachalene (2.0 %), epizonarene (0.8 %), trans-calamenene (4.4 %) and trans-cadina-1,4-diene (3.4 %) were identified for the first time as chemical constituents of essential oil from leaves of E. sulcata. To our knowledge, this was the first phytochemical contribution to the essential oil from stems of E. sulcata. It was also performed the acetylcholinesterase inhibitory bioassay of the...
American Journal of Hematology, 1997
LATIN AMERICAN JOURNAL OF PHARMACY
Alzheimer’s disease is a serious cognitive disorder with high impact, especially over older peopl... more Alzheimer’s disease is a serious cognitive disorder with high impact, especially over older people. The therapeutic approach for this disease involves inhibition of the acetylcholinesterase enzyme, but these drugs belong to the same chemical class and just relief the symptoms and provide no cure. On this context, discovering of new non-alkaloid drugs to inhibit this enzyme is an open field. Neomitranthes obscura (DC.) N. Silveira belongs to the family Myrtaceae and is restricted to Brazil. It is common known by as “camboim-de-cachorro” and to our knowledge, remains almost unexplored regarding its biological properties. The present study aimed to evaluate anticholinesterase activity of essential oil from N. obscura. In all, 17 compounds were identified and the essential oil presented an IC50 of 75.93 ± 0.85 μg/mL.
Parasitology research, 2014
A chemical investigation and bioassays against fifth-instar nymphae of the hematophagous insect R... more A chemical investigation and bioassays against fifth-instar nymphae of the hematophagous insect Rhodnius prolixus, vector of Chagas disease, were conducted with the essential oil from Zanthoxylum caribaeum. The main results may be summarized as follows: (i) 54 components were identified, corresponding to 90.4% of the relative composition; sesquiterpenes (47.3%) and monoterpenes (41.2%) are the major constituents; (ii) muurola-4,5-trans-diene and isodaucene are described for the first time as chemical constituents of the essential oil from leaves of this species; (iii) topical treatment with the crude essential oil induced high levels of paralysis (from 18.88 to 33.33%) and mortality (from 80 to 98.9%) depending on the dose applied (0.5 to 5.0 μl per insect); (iv) feeding treatment with the crude essential oil also induced high levels of mortality (from 48.8 to 100%) but low levels of paralysis (from 2.22 to 7.77%) depending on the dose applied (0.5 to 5.0 μl/ml of blood); (v) in the...
Cyanogenesis in sporophytes of pteridophytes evidenced by the picric acid test). Cyanogenic glyco... more Cyanogenesis in sporophytes of pteridophytes evidenced by the picric acid test). Cyanogenic glycosides are defense substances found in some plants that are able to produce cyanhydric acid on hydrolysis. The present work aimed to study the occ urrence of cyanogenesis in pteridophytes of rocky areas of Pedra de Itacoatiara, State Park of Serra da Tiririca, Rio de Janeiro State. Nineteen
Ethnobotany Research and Applications, 2015
Revista de Sociologia e Política, 2009
Redalyc. Red de Revistas Científicas de América Latina y el Caribe, España y Portugal Sistema de ... more Redalyc. Red de Revistas Científicas de América Latina y el Caribe, España y Portugal Sistema de Información Científica Redalyc Hemeroteca. ...
The Protein Journal, 2011
Plant Signaling & Behavior, 2009
Revista Brasileira de Farmacognosia
Myrciaria floribunda (H. West ex Willd.) O. Berg, Myrtaceae, is popularly known as “camboim-amare... more Myrciaria floribunda (H. West ex Willd.) O. Berg, Myrtaceae, is popularly known as “camboim-amarelo” and was collected at Restinga de Jurubatiba (RJ, Brazil). Leaves from this species were submitted to hydrodistillation to extract its essential oil. Monoterpenes were the main compounds found (53.9%), and 1,8-cineole was the major constituent (38.4%). Studies were carried out to evaluate the effects of this essential oil on the development of two species of agricultural pests (Oncopeltus fasciatus and Dysdercus peruvianus). The essential oil was considered effective against D. peruvianus and O. fasciatus, causing mortality in both insects. The LD50 values (μg/insect) observed were 112.44 μg/insect (O. fasciatus) and 309.64 μg/insect (D. peruvianus) after one day of treatment, and 72.18 μg/insect (O. fasciatus) and 94.42 μg/insect (D. peruvianus) after 22 days of treatment. The present study reports for the first time the bioinsecticidal activity of essential oil of Myrciaria floribun...
Revista Brasileira de Farmacognosia
Essential oils are known for their insect control potential, which is mainly attributed to the pr... more Essential oils are known for their insect control potential, which is mainly attributed to the presence of terpenes that interfere with hormonal and physiological processes of arthropods. The aim of the present study was to evaluate the effects of essential oil from the leaves of Eugenia sulcata Spring ex Mart., Myrtaceae, on the development of two species of agricultural pest insects, Dysdercus peruvianus and Oncopeltus fasciatus. Results showed that the essential oil induced mortality, and reduced numbers of adults. Topical treatment of Oncopeltus fasciatus using pure essential oil caused significant mortality rates (96.67%), while Dysdercus peruvianus had a higher tolerance, with 80% mortality at the end of the experiments. Results suggest that essential oil from the leaves of Eugenia sulcata may be used in agriculture for insect pest control.
Memórias do Instituto Oswaldo Cruz, 2015
Peptides, 2011
Antifungal proteins and peptides, essential compounds for plant defense, have been isolated from ... more Antifungal proteins and peptides, essential compounds for plant defense, have been isolated from several tissues of various plants. These proteins could be used as a natural alternative to control phytopathogenic fungi. In this report a heterodimeric antifungal protein named Pa-AFP1, showing higher identity with the 2S albumin family, was purified by using 70-100% ammonium sulfate saturation and further purification steps such as anionic exchange Q-Sepharose chromatography associated with HPLC reversed-phase C4 chromatography. Analysis by Tricine-SDS-PAGE revealed two peptidic molecular masses of approximately 4500 Da and 7000 Da, in the presence of β-mercaptoethanol, while by removing the reducing agent a single protein with molecular mass of about 11,500 Da was obtained. Moreover, dimer mass was confirmed by MALDI-TOF analyses (11,569.76 Da). The antifungal protein, named Pa-AFP1, efficiently inhibited the growth of filamentous fungi Colletotrichum gloeosporioides, and was added to a short list of 2S albumins with antimicrobial properties. Otherwise, this same peptide showed no activity toward bacteria and yeasts. In summary, this compound could be used in the future to develop biotechnological products for the control of phytopathogenic fungi.
Neomitranthes obscura (DC.) N. Silveira is an endemic plant of Brazilian Atlantic Forest and wide... more Neomitranthes obscura (DC.) N. Silveira is an endemic plant of Brazilian Atlantic Forest and widely spread in the sandbanks of "Restinga de Jurubatiba" National Park. It is popularly known by local population as "camboim-de-cachorro" or "cambuí-preto" and recognized by its black ripe fruits. However, specimens with yellow ripe fruits were localized in the "Restinga de Jurubatiba" National Park. The aim of the present study was to evaluate chemical composition of essential oils obtained from leaves and fruits of N. obscura specimens with different fruit color (black and yellow) by GC and GC-MS. Essential oils from leaves of specimens with black and yellow fruits indicated a predominance of sesquiterpenes (81.1% and 84.8%, resp.). Meanwhile, essential oil from black fruits presented a predominance of monoterpenes (50.5%), while essential oil from yellow fruits had sesquiterpenes (39.9%) as major substances. Despite previous studies about this sp...
In Vitro Cellular & Developmental Biology - Plant, 2006
LATIN AMERICAN JOURNAL OF PHARMACY
Manilkara subsericea (Mart.) Dubard (Sapotaceae) is popularly known in Brazil as “guracica”. The ... more Manilkara subsericea (Mart.) Dubard (Sapotaceae) is popularly known in Brazil as “guracica”. The species from the genus Manilkara have been studied and several substances were found, including triterpenes. In the present study, we describe the anticholinesterasic activity of an extract of M. subsericea containg beta-amyrin acetate (76.3 %) and alpha-amyrin acetate (23.7 %) using a TLC bioassay
Developmental & Comparative Immunology, 2011
LATIN AMERICAN JOURNAL OF PHARMACY
The chemical composition of the essential oils from leaves and stems of Eugenia sulcata Spring ex... more The chemical composition of the essential oils from leaves and stems of Eugenia sulcata Spring ex Mart., obtained by hydrodistillation, was analyzed by GC-MS and quantified by CG-FID. In all, 37 components were identified and sesquiterpenes represented the largest fraction of both oils, in the leaves (58.2 %) and stems (85.3 %). The major constituent found in the essential oil from leaves and stems of E. sulcata was β-caryophyllene, corresponding to 24.6 % and 18.8 %, respectively. The substances αcubebene (1.1 %), β-copaene (0.5 %), cis-muurola-3,5-diene (0.6 %), cis-muurola-4(14),5-diene (1.3 %), γhimachalene (2.0 %), epizonarene (0.8 %), trans-calamenene (4.4 %) and trans-cadina-1,4-diene (3.4 %) were identified for the first time as chemical constituents of essential oil from leaves of E. sulcata. To our knowledge, this was the first phytochemical contribution to the essential oil from stems of E. sulcata. It was also performed the acetylcholinesterase inhibitory bioassay of the...
American Journal of Hematology, 1997
LATIN AMERICAN JOURNAL OF PHARMACY
Alzheimer’s disease is a serious cognitive disorder with high impact, especially over older peopl... more Alzheimer’s disease is a serious cognitive disorder with high impact, especially over older people. The therapeutic approach for this disease involves inhibition of the acetylcholinesterase enzyme, but these drugs belong to the same chemical class and just relief the symptoms and provide no cure. On this context, discovering of new non-alkaloid drugs to inhibit this enzyme is an open field. Neomitranthes obscura (DC.) N. Silveira belongs to the family Myrtaceae and is restricted to Brazil. It is common known by as “camboim-de-cachorro” and to our knowledge, remains almost unexplored regarding its biological properties. The present study aimed to evaluate anticholinesterase activity of essential oil from N. obscura. In all, 17 compounds were identified and the essential oil presented an IC50 of 75.93 ± 0.85 μg/mL.
Parasitology research, 2014
A chemical investigation and bioassays against fifth-instar nymphae of the hematophagous insect R... more A chemical investigation and bioassays against fifth-instar nymphae of the hematophagous insect Rhodnius prolixus, vector of Chagas disease, were conducted with the essential oil from Zanthoxylum caribaeum. The main results may be summarized as follows: (i) 54 components were identified, corresponding to 90.4% of the relative composition; sesquiterpenes (47.3%) and monoterpenes (41.2%) are the major constituents; (ii) muurola-4,5-trans-diene and isodaucene are described for the first time as chemical constituents of the essential oil from leaves of this species; (iii) topical treatment with the crude essential oil induced high levels of paralysis (from 18.88 to 33.33%) and mortality (from 80 to 98.9%) depending on the dose applied (0.5 to 5.0 μl per insect); (iv) feeding treatment with the crude essential oil also induced high levels of mortality (from 48.8 to 100%) but low levels of paralysis (from 2.22 to 7.77%) depending on the dose applied (0.5 to 5.0 μl/ml of blood); (v) in the...
Cyanogenesis in sporophytes of pteridophytes evidenced by the picric acid test). Cyanogenic glyco... more Cyanogenesis in sporophytes of pteridophytes evidenced by the picric acid test). Cyanogenic glycosides are defense substances found in some plants that are able to produce cyanhydric acid on hydrolysis. The present work aimed to study the occ urrence of cyanogenesis in pteridophytes of rocky areas of Pedra de Itacoatiara, State Park of Serra da Tiririca, Rio de Janeiro State. Nineteen
Ethnobotany Research and Applications, 2015