Aleksandar Djordjevic | University of Novi Sad, Faculty of Economics Subotica (original) (raw)

Papers by Aleksandar Djordjevic

Over the past few years, significant efforts have been made to decrease the effects of drought st... more Over the past few years, significant efforts have been made to decrease the effects of drought stress on plant productivity and quality. We propose that fullerenol nanoparticles (FNPs, molecular formula C 60 (OH) 24) may help alleviate drought stress by serving as an additional intercellular water supply. Specifically, FNPs are able to penetrate plant leaf and root tissues, where they bind water in various cell compartments. This hydroscopic activity suggests that FNPs could be beneficial in plants. The aim of the present study was to analyse the influence of FNPs on sugar beet plants exposed to drought stress. Our results indicate that intracellular water metabolism can be modified by foliar application of FNPs in drought exposed plants. Drought stress induced a significant increase in the compatible osmolyte proline in both the leaves and roots of control plants, but not in FNP treated plants. These results indicate that FNPs could act as intracellular binders of water, creating an additional water reserve, and enabling adaptation to drought stress. Moreover, analysis of plant antioxidant enzyme activities (CAT, APx and GPx), MDA and GSH content indicate that full-erenol foliar application could have some beneficial effect on alleviating oxidative effects of drought stress, depending on the concentration of nanoparticles applied. Although further studies are necessary to elucidate the biochemical impact of FNPs on plants; the present results could directly impact agricultural practice, where available water supplies are often a limiting factor in plant bioproductivity.

The Scientific World Journal, 2015

A comprehensive comparative analysis of antifungal potential of benzalkonium chloride and newly s... more A comprehensive comparative analysis of antifungal potential of benzalkonium chloride and newly synthesized fullerenol/benzalkonium chloride nanocomposite was conducted to assess the possible impact of carbon-based nanocarrier on antimicrobial properties of the commonly used biocide. Physical characterization of synthesized nanocomposite showed zeta potential of +37.4 mV and inhomogeneous particles size distribution, with nanocomposite particles' dimensions within 30-143 nm and maximum number of particles at 44 nm. The effect of pure and fullerenol nanocarrier-bound biocide was evaluated in eight Aspergillus species. In mycelial growth assay, nanocomposite was more potent, as fungicidal effect of 1.04/0.6 μg mL(-1) was obtained in all but one of the isolates (A. niger), while proportional concentration of pure biocide (0.6 μg mL(-1)) completely inhibited mycelial growth of only three Aspergillus species. However, conidia appear to be less susceptible to nanocomposite treatment, as lower fungistatic (MIC) and fungicidal (MFC) concentrations were obtained with biocide alone (MIC in range from 0.03 to 0.15 μg mL(-1) and MFC from 0.075 to 0.45 μg mL(-1)). To a different degree, both substances stimulated aflatoxin B1 production and inhibited ochratoxin A synthesis. Very low mycelium biomass yield, in range from 1.0 to 3.0 mg dry weight, was documented in both biocide and nanocomposite enriched medium.

Carbon nanomaterials have been the subject of extensive research over the past decade because of ... more Carbon nanomaterials have been the subject of extensive research over the past decade because of their use in many commercial products as well as their vast application in agriculture, medicine, electronic industry, cosmetics and others [1]. The molecules of fulleren C60 are by their nature hydrophobic and in water they tend to form nano aggregates (nC60). The high adsorptive capacity of nC60 can have an impact on transformation and transport of other hydrophobic organic compounds such as naphthalene, phenanthrene, pyrene, pesticides and others [2]. According to US National Toxicology Program’s twelfth report, chloroform is classified as carcinogen and is formed together with other disinfection by-products during water chlorination [3]. Suspended nano particles can either increase or decrease bioaccumulation of disinfection by-products [4]. Digestive tract of Daphnia magna is well known model system for toxicity evaluation of many diverse nano particles. Considering that nC60 can ch...

Advances in Materials Science and Engineering, 2015

Novel nanocomposite containing fullerenol nanoparticles (FNP) and porous silica nanoparticles (PS... more Novel nanocomposite containing fullerenol nanoparticles (FNP) and porous silica nanoparticles (PSNs) was constructed and characterized. The capability of FNP to serve as a pore-capping agent and for entrapping 9-aminoacridine (9-AA) inside the pores of the PSN material was also demonstrated. Nitrogen sorption measurements evidence the successful capping of the silica pores while thermogravimetric analysis of FNP loaded PSN indicates the existence of pore-loaded fullerenol molecules. Higher amount of the drug release was noted by exposing the material to weakly acidic conditions in comparison to physiological pH, which may find application in targeted treatment of weakly acidic tumor tissues.

To examine the difference in the ability of mouse fibroblasts (L929) to adhere to discs of hydrox... more To examine the difference in the ability of mouse fibroblasts (L929) to adhere to discs of hydroxyapatite (HAp) and hydroxyapatite/fullerenol nanocomposite (HAp/FNP)

Pharmacological Reports, 2009

The aim of this study was to investigate the possible protective role of fullerenol (FLR, C 60 (O... more The aim of this study was to investigate the possible protective role of fullerenol (FLR, C 60 (OH) 24 on doxorubicin (DOX)-induced lung toxicity using biochemical and histopathological approaches. Rats (Sprague-Dawley outbred) were randomly divided into five groups. The healthy control group received no medication (saline only). The other four groups had chemically induced breast cancer (1-methyl-1-nitrosourea; 50 mg/kg, ip). The second group was the cancer control group (saline only). The other three groups were DOX (8 mg/kg, ip), FLR/DOX (100 mg/kg, ip, 30 min before DOX; 8 mg/kg, ip), and FLR (100 mg/kg, ip), respectively. The levels of malondialdehyde (MDA) and oxidized glutathione (GSSG) in the lung tissue were higher in the group treated with DOX alone than in the control groups. The activities of catalase (CAT), glutathione reductase (GR), superoxide dismutase (SOD), and lactate dehydrogenase (LDH) were found to be increased in the lung tissue of the animals in the DOX group over all the other groups, while GSH-Px significantly decreased in activity compared with the control and FLR groups. There was no significant difference in MDA and GSSG levels and enzyme activities in either control (healthy; cancer) or FLR (FLR/DOX; FLR) groups. The acute change found in the DOX group was subpleural edema. In contrast, the groups treated with FLR appeared to be virtually histopathologically normal. In conclusion, this study clearly indicates that DOX treatment markedly impairs pulmonary function and that pre-treatment with FLR might prevent this toxicity in rats through inhibition of oxidative stress.

Digest Journal of Nanomaterials and Biostructures

Cited By (since 1996):1, Export Date: 18 October 2014

Oxidation Communications

The antioxidant properties of open cage water-soluble fullerenol C60(OH)24 were tested by measuri... more The antioxidant properties of open cage water-soluble fullerenol C60(OH)24 were tested by measuring their ability to scavenge stable 2,2-diphenyl-1-picryl¬hy¬dra¬zyl (DPPH) free radical and reactive hydroxyl radical (∙OH) during the Fenton reaction using electron spin resonance (ESR) spectroscopy. The fullerenol, obtained by newly developed method in our laboratory, scavenged bouth type of investigated radicals in dose-dependent manner in concentration range of 0.18-0.88 mmol/l. Generally, higher concentrations (0.71-0.88 mmol/l) of fullerenol possessed better inhibition of hydroxyl radical (50-82%) than of DPPH radical (28-50%). Fullerenol can exhibit its antioxidative properties by simultaneous hydrogen atom donation to investigated free radicals (DPPH and ∙OH). This reaction also gave rise to the generation of the fullerenol radical C60(OH)23O. which presence was confirmed by ESR. Interaction between hydroxyl radical and fullerenol also based on radical-addition reaction of 2n .O...

Digest Journal of Nanomaterials and Biostructures

Water soluble fullerene (C60) derivative fullerenol C60(OH)24 nano-particles (FNP) are a promisin... more Water soluble fullerene (C60) derivative fullerenol C60(OH)24 nano-particles (FNP) are a promising candidate for many biomedical applications due to, besides other properties, strong free-radical scavenging and antioxidative potential. Using the carrageenan-induced rat footpad oedema test, the anti-inflammatory effect of FNP have been estimated in comparison with those of amifostine (AMI) and indomethacin (IND). FNP and IND, dissolved in dimethylsulfoxide, and AMI, dissolved in saline, were intraperitoneally injected to rats in a dose range 12.5 - 75 mg/kg, 3 - 10 mg/kg, and 50 - 300 mg/kg, respectively. The control groups were given corresponding vehicles. The drugs or vehicles were given 30 min before carrageenan injection. Footpad swelling was measured 3 hours after carrageenan application. Calculation of a per cent of inhibition derived through comparison with the control groups was done. Histopathological examination of the inflamed foot skin biopsies was also performed. FNP do...

Materials Science Forum, 2004

Intercalates of C 60 were obtained by a direct synthesis of C 60 solution in xylene, ethylbenzene... more Intercalates of C 60 were obtained by a direct synthesis of C 60 solution in xylene, ethylbenzene, n-propylbenzene, n-butylbenzene and terc-butylbenzene with methanol, ethanol and isopropanol, respectively -"guest". Alcohol-solvent system interacts with C 60 and forms binary and ternary isostructure intercalate products. Infrared spectra of all derivatives showed no change compared to the pure C 60 . Binary intercalates are isostructural with the C 60 crystal structure , the same as ternary intercalates synthesized from a saturated solution of C 60 in xylene, n-butylbenzene and terc-butylbenzene. Intercalates do not have stoichiometric relation alcohol : C 60 . The presence of more voluminous solvents (terc-butylbenzene, xylene and n-butylbenzene) in ternary intercalates of C 60 affects the changes in intermolecular space of the C 60 crystal structure as well as the formation of new intermolecular interactions. In all cases of binary and ternary intercalated systems, alkyl-substituted benzene played the role of "donor" of the intercalating agent -"guest" in the C 60 crystal structures.

Nitric Oxide, 2004

Investigation of the possible nitric oxide-scavenging activity of hydroxylated derivative of full... more Investigation of the possible nitric oxide-scavenging activity of hydroxylated derivative of fullerene, fullerenol C 60 (OH) 24 , demonstrated that it expressed direct scavenging activity toward nitric oxide radical (NO) liberated within solution of sodium nitroprusside (SNP), a well known NO donor. In parallel, pre-treatment (30 0 ) with intratesticular injection of fullerenol (60 lg/each testis) prevented NO-induced decrease of catalase, glutathione transferase and glutathione peroxidase activities in the denucleated fraction of interstitial testicular cells of adult rats 2 h after intratesticular injection of SNP (20 lg/each testis). In addition, fullerenol decreased formation of thiobarbituric acid-reactive substances (TBA-RS) with similar efficiency as butylated hydroxy toluen (BHT), a well known antioxidant. Also, fullerenol expressed certain scavenging activity toward superoxide anion ðO À 2 Þ in xanthine/xanthine oxidase system. In summary, results obtained in this study confirmed free radical-scavenging activity of fullerenol, and according to our knowledge, it is the first evidence of direct NO-quenching activity of hydroxylated C 60 derivative in different milieu.

Tiwari/Advanced, 2013

ABSTRACT This chapter contains sections titled: Biologically Active Fullerene Derivatives Biologi... more ABSTRACT This chapter contains sections titled: Biologically Active Fullerene Derivatives Biologically Active Graphene Materials Bioimpact of Carbon Nanotubes Genotoxicity of Carbon Nanomaterials Ecotoxicological Effects of Carbon Nanomaterials

Ultrastructural Pathology, 2014

Cardioprotective effects of fullerenol C 60 (OH) 24 nanoparticles (FNP) were investigated in pigs... more Cardioprotective effects of fullerenol C 60 (OH) 24 nanoparticles (FNP) were investigated in pigs after a single treatment with doxorubicin (DOX). Semithin and ultrathin sections of myocardial tissue routinely prepared for transmission electron microscopy were analyzed. Extensive intracellular damage was confirmed in cardiomyocytes of DOX-treated animals. By means of ultrastructural analysis, a certain degree of parenchymal degeneration was confirmed even in animals treated with FNP alone, including both the oral and the intraperitoneal application of the substance. The cardioprotective effects of FNP in animals previously treated with DOX were recognized to a certain extent, but were not fully confirmed at the ultrastructural level. Nevertheless, the myocardial morphology of DOX-treated animals improved after the admission of FNP. Irregular orientation of myofibrils, myofibrillar disruption, intracellular edema, and vacuolization were reduced, but not completely eliminated. Reduction of these cellular alterations was achieved if FNP was applied orally 6 h prior to DOX treatment in a dose of 18 mg/kg. However, numerous defects, including the inner mitochondrial membrane and the plasma membrane disruption of certain cells persisted. In FNP/DOXtreated animals, the presence of multinuclear cells with mitosis-like figures resembling metaphase or anaphase were observed, indicating that DOX and FNP could have a complex influence on the cell cycle of cardiomyocytes. Based on this experiment, further careful increase in dosage may be advised to enhance FNPinduced cardioprotection. These investigations should, however, always be combined with ultrastructural analysis. The FNP/DOX interaction is an excellent model for the investigation of cardiomyocyte cell death and cell cycle mechanisms.

Pharmacological Reports, 2009

The aim of this study was to investigate the possible protective role of fullerenol (FLR, C 60 (O... more The aim of this study was to investigate the possible protective role of fullerenol (FLR, C 60 (OH) 24 on doxorubicin (DOX)-induced lung toxicity using biochemical and histopathological approaches. Rats (Sprague-Dawley outbred) were randomly divided into five groups. The healthy control group received no medication (saline only). The other four groups had chemically induced breast cancer (1-methyl-1-nitrosourea; 50 mg/kg, ip). The second group was the cancer control group (saline only). The other three groups were DOX (8 mg/kg, ip), FLR/DOX (100 mg/kg, ip, 30 min before DOX; 8 mg/kg, ip), and FLR (100 mg/kg, ip), respectively. The levels of malondialdehyde (MDA) and oxidized glutathione (GSSG) in the lung tissue were higher in the group treated with DOX alone than in the control groups. The activities of catalase (CAT), glutathione reductase (GR), superoxide dismutase (SOD), and lactate dehydrogenase (LDH) were found to be increased in the lung tissue of the animals in the DOX group over all the other groups, while GSH-Px significantly decreased in activity compared with the control and FLR groups. There was no significant difference in MDA and GSSG levels and enzyme activities in either control (healthy; cancer) or FLR (FLR/DOX; FLR) groups. The acute change found in the DOX group was subpleural edema. In contrast, the groups treated with FLR appeared to be virtually histopathologically normal. In conclusion, this study clearly indicates that DOX treatment markedly impairs pulmonary function and that pre-treatment with FLR might prevent this toxicity in rats through inhibition of oxidative stress.

Journal of Molecular Structure, 1998

New non-covalent intermolecular fullerene system containing discrete C 60 and alcohol molecules h... more New non-covalent intermolecular fullerene system containing discrete C 60 and alcohol molecules have been prepared from the saturated solutions of fullerene in toluene with ethanol, methanol and isopropanol.

Journal of the American Oil Chemists Society, 1991

The ethanolic extract of sage {Salvia officinalis L.} was separated into five fractions through r... more The ethanolic extract of sage {Salvia officinalis L.} was separated into five fractions through reextraction with supercritical CO2. Further fractionation of the most active antioxidant fractions by means of liquid chromatography, with silicic acid as absorbent, yielded 2H-10,4a-(epoxy methano}-phenantren-12-one-l,3,4,9,10,10ahexahydro-5,6-dihydroxy-9a-ethoxy-1,1-dimethyl-7-(1methylethyl), (rosmanol-9-ethyl ether}. The same compound was isolated from the alcoholic extract of the hyssop {Hyssopus officinalis L.}. Rosmanol-9-ethyl ether was shown to be one of the active antioxidant components in sage and hyssop, with activity much greater than butylated hydroxytoluene (BHT}.

Fullerene Science and Technology, 1998

In this paper the procedure for catalytical bromination of C 60 with elementary bromine with FeBr... more In this paper the procedure for catalytical bromination of C 60 with elementary bromine with FeBr 3 as a catalyst is described. In this procedures only one reaction product -C 60 Br 24 is obtained. The twenty four bromine atoms are symmetrically distributed over the C 60 sphere, which was confirmed by thermogravimetric analysis. The yield of bromine derivative in this reaction is 98%.

Colloids and Surfaces B: Biointerfaces, 2007

Polyhydroxylated fullerenes, named fullerenols (C 60 (OH) n ; n = 12-26) are excellent antioxidan... more Polyhydroxylated fullerenes, named fullerenols (C 60 (OH) n ; n = 12-26) are excellent antioxidants. Harmful effects of ionizing radiation on living organism are mainly mediated by free radical species and fullerenols attract an attention as a potential radioprotectors. Our preliminary investigations on mice and rats subjected to radiation injury show that fullerenol C 60 (OH) 24 provides high survival rate of irradiated small rodents. Radioprotective effect was comparable to that of the standard radioprotector amifostine.

Over the past few years, significant efforts have been made to decrease the effects of drought st... more Over the past few years, significant efforts have been made to decrease the effects of drought stress on plant productivity and quality. We propose that fullerenol nanoparticles (FNPs, molecular formula C 60 (OH) 24) may help alleviate drought stress by serving as an additional intercellular water supply. Specifically, FNPs are able to penetrate plant leaf and root tissues, where they bind water in various cell compartments. This hydroscopic activity suggests that FNPs could be beneficial in plants. The aim of the present study was to analyse the influence of FNPs on sugar beet plants exposed to drought stress. Our results indicate that intracellular water metabolism can be modified by foliar application of FNPs in drought exposed plants. Drought stress induced a significant increase in the compatible osmolyte proline in both the leaves and roots of control plants, but not in FNP treated plants. These results indicate that FNPs could act as intracellular binders of water, creating an additional water reserve, and enabling adaptation to drought stress. Moreover, analysis of plant antioxidant enzyme activities (CAT, APx and GPx), MDA and GSH content indicate that full-erenol foliar application could have some beneficial effect on alleviating oxidative effects of drought stress, depending on the concentration of nanoparticles applied. Although further studies are necessary to elucidate the biochemical impact of FNPs on plants; the present results could directly impact agricultural practice, where available water supplies are often a limiting factor in plant bioproductivity.

The Scientific World Journal, 2015

A comprehensive comparative analysis of antifungal potential of benzalkonium chloride and newly s... more A comprehensive comparative analysis of antifungal potential of benzalkonium chloride and newly synthesized fullerenol/benzalkonium chloride nanocomposite was conducted to assess the possible impact of carbon-based nanocarrier on antimicrobial properties of the commonly used biocide. Physical characterization of synthesized nanocomposite showed zeta potential of +37.4 mV and inhomogeneous particles size distribution, with nanocomposite particles' dimensions within 30-143 nm and maximum number of particles at 44 nm. The effect of pure and fullerenol nanocarrier-bound biocide was evaluated in eight Aspergillus species. In mycelial growth assay, nanocomposite was more potent, as fungicidal effect of 1.04/0.6 μg mL(-1) was obtained in all but one of the isolates (A. niger), while proportional concentration of pure biocide (0.6 μg mL(-1)) completely inhibited mycelial growth of only three Aspergillus species. However, conidia appear to be less susceptible to nanocomposite treatment, as lower fungistatic (MIC) and fungicidal (MFC) concentrations were obtained with biocide alone (MIC in range from 0.03 to 0.15 μg mL(-1) and MFC from 0.075 to 0.45 μg mL(-1)). To a different degree, both substances stimulated aflatoxin B1 production and inhibited ochratoxin A synthesis. Very low mycelium biomass yield, in range from 1.0 to 3.0 mg dry weight, was documented in both biocide and nanocomposite enriched medium.

Carbon nanomaterials have been the subject of extensive research over the past decade because of ... more Carbon nanomaterials have been the subject of extensive research over the past decade because of their use in many commercial products as well as their vast application in agriculture, medicine, electronic industry, cosmetics and others [1]. The molecules of fulleren C60 are by their nature hydrophobic and in water they tend to form nano aggregates (nC60). The high adsorptive capacity of nC60 can have an impact on transformation and transport of other hydrophobic organic compounds such as naphthalene, phenanthrene, pyrene, pesticides and others [2]. According to US National Toxicology Program’s twelfth report, chloroform is classified as carcinogen and is formed together with other disinfection by-products during water chlorination [3]. Suspended nano particles can either increase or decrease bioaccumulation of disinfection by-products [4]. Digestive tract of Daphnia magna is well known model system for toxicity evaluation of many diverse nano particles. Considering that nC60 can ch...

Advances in Materials Science and Engineering, 2015

Novel nanocomposite containing fullerenol nanoparticles (FNP) and porous silica nanoparticles (PS... more Novel nanocomposite containing fullerenol nanoparticles (FNP) and porous silica nanoparticles (PSNs) was constructed and characterized. The capability of FNP to serve as a pore-capping agent and for entrapping 9-aminoacridine (9-AA) inside the pores of the PSN material was also demonstrated. Nitrogen sorption measurements evidence the successful capping of the silica pores while thermogravimetric analysis of FNP loaded PSN indicates the existence of pore-loaded fullerenol molecules. Higher amount of the drug release was noted by exposing the material to weakly acidic conditions in comparison to physiological pH, which may find application in targeted treatment of weakly acidic tumor tissues.

To examine the difference in the ability of mouse fibroblasts (L929) to adhere to discs of hydrox... more To examine the difference in the ability of mouse fibroblasts (L929) to adhere to discs of hydroxyapatite (HAp) and hydroxyapatite/fullerenol nanocomposite (HAp/FNP)

Pharmacological Reports, 2009

The aim of this study was to investigate the possible protective role of fullerenol (FLR, C 60 (O... more The aim of this study was to investigate the possible protective role of fullerenol (FLR, C 60 (OH) 24 on doxorubicin (DOX)-induced lung toxicity using biochemical and histopathological approaches. Rats (Sprague-Dawley outbred) were randomly divided into five groups. The healthy control group received no medication (saline only). The other four groups had chemically induced breast cancer (1-methyl-1-nitrosourea; 50 mg/kg, ip). The second group was the cancer control group (saline only). The other three groups were DOX (8 mg/kg, ip), FLR/DOX (100 mg/kg, ip, 30 min before DOX; 8 mg/kg, ip), and FLR (100 mg/kg, ip), respectively. The levels of malondialdehyde (MDA) and oxidized glutathione (GSSG) in the lung tissue were higher in the group treated with DOX alone than in the control groups. The activities of catalase (CAT), glutathione reductase (GR), superoxide dismutase (SOD), and lactate dehydrogenase (LDH) were found to be increased in the lung tissue of the animals in the DOX group over all the other groups, while GSH-Px significantly decreased in activity compared with the control and FLR groups. There was no significant difference in MDA and GSSG levels and enzyme activities in either control (healthy; cancer) or FLR (FLR/DOX; FLR) groups. The acute change found in the DOX group was subpleural edema. In contrast, the groups treated with FLR appeared to be virtually histopathologically normal. In conclusion, this study clearly indicates that DOX treatment markedly impairs pulmonary function and that pre-treatment with FLR might prevent this toxicity in rats through inhibition of oxidative stress.

Digest Journal of Nanomaterials and Biostructures

Cited By (since 1996):1, Export Date: 18 October 2014

Oxidation Communications

The antioxidant properties of open cage water-soluble fullerenol C60(OH)24 were tested by measuri... more The antioxidant properties of open cage water-soluble fullerenol C60(OH)24 were tested by measuring their ability to scavenge stable 2,2-diphenyl-1-picryl¬hy¬dra¬zyl (DPPH) free radical and reactive hydroxyl radical (∙OH) during the Fenton reaction using electron spin resonance (ESR) spectroscopy. The fullerenol, obtained by newly developed method in our laboratory, scavenged bouth type of investigated radicals in dose-dependent manner in concentration range of 0.18-0.88 mmol/l. Generally, higher concentrations (0.71-0.88 mmol/l) of fullerenol possessed better inhibition of hydroxyl radical (50-82%) than of DPPH radical (28-50%). Fullerenol can exhibit its antioxidative properties by simultaneous hydrogen atom donation to investigated free radicals (DPPH and ∙OH). This reaction also gave rise to the generation of the fullerenol radical C60(OH)23O. which presence was confirmed by ESR. Interaction between hydroxyl radical and fullerenol also based on radical-addition reaction of 2n .O...

Digest Journal of Nanomaterials and Biostructures

Water soluble fullerene (C60) derivative fullerenol C60(OH)24 nano-particles (FNP) are a promisin... more Water soluble fullerene (C60) derivative fullerenol C60(OH)24 nano-particles (FNP) are a promising candidate for many biomedical applications due to, besides other properties, strong free-radical scavenging and antioxidative potential. Using the carrageenan-induced rat footpad oedema test, the anti-inflammatory effect of FNP have been estimated in comparison with those of amifostine (AMI) and indomethacin (IND). FNP and IND, dissolved in dimethylsulfoxide, and AMI, dissolved in saline, were intraperitoneally injected to rats in a dose range 12.5 - 75 mg/kg, 3 - 10 mg/kg, and 50 - 300 mg/kg, respectively. The control groups were given corresponding vehicles. The drugs or vehicles were given 30 min before carrageenan injection. Footpad swelling was measured 3 hours after carrageenan application. Calculation of a per cent of inhibition derived through comparison with the control groups was done. Histopathological examination of the inflamed foot skin biopsies was also performed. FNP do...

Materials Science Forum, 2004

Intercalates of C 60 were obtained by a direct synthesis of C 60 solution in xylene, ethylbenzene... more Intercalates of C 60 were obtained by a direct synthesis of C 60 solution in xylene, ethylbenzene, n-propylbenzene, n-butylbenzene and terc-butylbenzene with methanol, ethanol and isopropanol, respectively -"guest". Alcohol-solvent system interacts with C 60 and forms binary and ternary isostructure intercalate products. Infrared spectra of all derivatives showed no change compared to the pure C 60 . Binary intercalates are isostructural with the C 60 crystal structure , the same as ternary intercalates synthesized from a saturated solution of C 60 in xylene, n-butylbenzene and terc-butylbenzene. Intercalates do not have stoichiometric relation alcohol : C 60 . The presence of more voluminous solvents (terc-butylbenzene, xylene and n-butylbenzene) in ternary intercalates of C 60 affects the changes in intermolecular space of the C 60 crystal structure as well as the formation of new intermolecular interactions. In all cases of binary and ternary intercalated systems, alkyl-substituted benzene played the role of "donor" of the intercalating agent -"guest" in the C 60 crystal structures.

Nitric Oxide, 2004

Investigation of the possible nitric oxide-scavenging activity of hydroxylated derivative of full... more Investigation of the possible nitric oxide-scavenging activity of hydroxylated derivative of fullerene, fullerenol C 60 (OH) 24 , demonstrated that it expressed direct scavenging activity toward nitric oxide radical (NO) liberated within solution of sodium nitroprusside (SNP), a well known NO donor. In parallel, pre-treatment (30 0 ) with intratesticular injection of fullerenol (60 lg/each testis) prevented NO-induced decrease of catalase, glutathione transferase and glutathione peroxidase activities in the denucleated fraction of interstitial testicular cells of adult rats 2 h after intratesticular injection of SNP (20 lg/each testis). In addition, fullerenol decreased formation of thiobarbituric acid-reactive substances (TBA-RS) with similar efficiency as butylated hydroxy toluen (BHT), a well known antioxidant. Also, fullerenol expressed certain scavenging activity toward superoxide anion ðO À 2 Þ in xanthine/xanthine oxidase system. In summary, results obtained in this study confirmed free radical-scavenging activity of fullerenol, and according to our knowledge, it is the first evidence of direct NO-quenching activity of hydroxylated C 60 derivative in different milieu.

Tiwari/Advanced, 2013

ABSTRACT This chapter contains sections titled: Biologically Active Fullerene Derivatives Biologi... more ABSTRACT This chapter contains sections titled: Biologically Active Fullerene Derivatives Biologically Active Graphene Materials Bioimpact of Carbon Nanotubes Genotoxicity of Carbon Nanomaterials Ecotoxicological Effects of Carbon Nanomaterials

Ultrastructural Pathology, 2014

Cardioprotective effects of fullerenol C 60 (OH) 24 nanoparticles (FNP) were investigated in pigs... more Cardioprotective effects of fullerenol C 60 (OH) 24 nanoparticles (FNP) were investigated in pigs after a single treatment with doxorubicin (DOX). Semithin and ultrathin sections of myocardial tissue routinely prepared for transmission electron microscopy were analyzed. Extensive intracellular damage was confirmed in cardiomyocytes of DOX-treated animals. By means of ultrastructural analysis, a certain degree of parenchymal degeneration was confirmed even in animals treated with FNP alone, including both the oral and the intraperitoneal application of the substance. The cardioprotective effects of FNP in animals previously treated with DOX were recognized to a certain extent, but were not fully confirmed at the ultrastructural level. Nevertheless, the myocardial morphology of DOX-treated animals improved after the admission of FNP. Irregular orientation of myofibrils, myofibrillar disruption, intracellular edema, and vacuolization were reduced, but not completely eliminated. Reduction of these cellular alterations was achieved if FNP was applied orally 6 h prior to DOX treatment in a dose of 18 mg/kg. However, numerous defects, including the inner mitochondrial membrane and the plasma membrane disruption of certain cells persisted. In FNP/DOXtreated animals, the presence of multinuclear cells with mitosis-like figures resembling metaphase or anaphase were observed, indicating that DOX and FNP could have a complex influence on the cell cycle of cardiomyocytes. Based on this experiment, further careful increase in dosage may be advised to enhance FNPinduced cardioprotection. These investigations should, however, always be combined with ultrastructural analysis. The FNP/DOX interaction is an excellent model for the investigation of cardiomyocyte cell death and cell cycle mechanisms.

Pharmacological Reports, 2009

The aim of this study was to investigate the possible protective role of fullerenol (FLR, C 60 (O... more The aim of this study was to investigate the possible protective role of fullerenol (FLR, C 60 (OH) 24 on doxorubicin (DOX)-induced lung toxicity using biochemical and histopathological approaches. Rats (Sprague-Dawley outbred) were randomly divided into five groups. The healthy control group received no medication (saline only). The other four groups had chemically induced breast cancer (1-methyl-1-nitrosourea; 50 mg/kg, ip). The second group was the cancer control group (saline only). The other three groups were DOX (8 mg/kg, ip), FLR/DOX (100 mg/kg, ip, 30 min before DOX; 8 mg/kg, ip), and FLR (100 mg/kg, ip), respectively. The levels of malondialdehyde (MDA) and oxidized glutathione (GSSG) in the lung tissue were higher in the group treated with DOX alone than in the control groups. The activities of catalase (CAT), glutathione reductase (GR), superoxide dismutase (SOD), and lactate dehydrogenase (LDH) were found to be increased in the lung tissue of the animals in the DOX group over all the other groups, while GSH-Px significantly decreased in activity compared with the control and FLR groups. There was no significant difference in MDA and GSSG levels and enzyme activities in either control (healthy; cancer) or FLR (FLR/DOX; FLR) groups. The acute change found in the DOX group was subpleural edema. In contrast, the groups treated with FLR appeared to be virtually histopathologically normal. In conclusion, this study clearly indicates that DOX treatment markedly impairs pulmonary function and that pre-treatment with FLR might prevent this toxicity in rats through inhibition of oxidative stress.

Journal of Molecular Structure, 1998

New non-covalent intermolecular fullerene system containing discrete C 60 and alcohol molecules h... more New non-covalent intermolecular fullerene system containing discrete C 60 and alcohol molecules have been prepared from the saturated solutions of fullerene in toluene with ethanol, methanol and isopropanol.

Journal of the American Oil Chemists Society, 1991

The ethanolic extract of sage {Salvia officinalis L.} was separated into five fractions through r... more The ethanolic extract of sage {Salvia officinalis L.} was separated into five fractions through reextraction with supercritical CO2. Further fractionation of the most active antioxidant fractions by means of liquid chromatography, with silicic acid as absorbent, yielded 2H-10,4a-(epoxy methano}-phenantren-12-one-l,3,4,9,10,10ahexahydro-5,6-dihydroxy-9a-ethoxy-1,1-dimethyl-7-(1methylethyl), (rosmanol-9-ethyl ether}. The same compound was isolated from the alcoholic extract of the hyssop {Hyssopus officinalis L.}. Rosmanol-9-ethyl ether was shown to be one of the active antioxidant components in sage and hyssop, with activity much greater than butylated hydroxytoluene (BHT}.

Fullerene Science and Technology, 1998

In this paper the procedure for catalytical bromination of C 60 with elementary bromine with FeBr... more In this paper the procedure for catalytical bromination of C 60 with elementary bromine with FeBr 3 as a catalyst is described. In this procedures only one reaction product -C 60 Br 24 is obtained. The twenty four bromine atoms are symmetrically distributed over the C 60 sphere, which was confirmed by thermogravimetric analysis. The yield of bromine derivative in this reaction is 98%.

Colloids and Surfaces B: Biointerfaces, 2007

Polyhydroxylated fullerenes, named fullerenols (C 60 (OH) n ; n = 12-26) are excellent antioxidan... more Polyhydroxylated fullerenes, named fullerenols (C 60 (OH) n ; n = 12-26) are excellent antioxidants. Harmful effects of ionizing radiation on living organism are mainly mediated by free radical species and fullerenols attract an attention as a potential radioprotectors. Our preliminary investigations on mice and rats subjected to radiation injury show that fullerenol C 60 (OH) 24 provides high survival rate of irradiated small rodents. Radioprotective effect was comparable to that of the standard radioprotector amifostine.