Juliana Correia | FCUL - Academia.edu (original) (raw)

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Papers by Juliana Correia

The purpose of this study focused on the assessment of the potential of natural extracts from veg... more The purpose of this study focused on the assessment of the potential of natural extracts from vegetable biomass of the species Acacia melanoxylon R. Br. to obtain compounds of industrial interest, such as antioxidants and anti-inflammatory compounds, enhancing these wastes and making them economically viable. The experimental work used the hydrodistillation method with a Clevenger apparatus for the aerial parts (bark, leaves and branches) of Acacia melanoxylon species, to obtain essential oils. The residual decoction waters were evaluated for their phenolic composition. The decoction waters were evaluated for the total phenolic compounds, tannins and non-tannins by the Folin-Ciocalteau method using gallic acid as standard, expressing the results in GAE (gallic acid equivalents). The antioxidant activity was determined by the free radical DPPH • capture method and the ABTS •+ method, using Trolox as standard. Further characterization was done by fractionation of the extracts using column chromatography and thin layer chromatography. Fractions obtained were analyzed by CZE, having identified different phenolic compounds from simple to complex structures, some of which have never been reported for the genus Acacia. Finally, the anti-inflammatory activity was determined by the bovine serum albumin (BSA) inhibition method, using acetyl salicylic acid as standard. To the best of our knowledge, this is the first time that phenolic acids and flavonoids were identified in the aqueous extract of leaves/branches and bark of Acacia melanoxylon, and that the presence of O-acetylsalisylic acid is referred in Acacia species. This work allowed, not only to supplement the existing phytochemical study for this species, but also to evaluate the potential of the bioactive phenolic compounds identified, enhancing thus the valorization of biomass as an abundant source of natural compounds with pharmacological activity. It is expected to continue these lines of research for development of value added products based on these bioactive compounds useful for the food, pharmaceutical and chemical industries.

The purpose of this study focused on the assessment of the potential of natural extracts from veg... more The purpose of this study focused on the assessment of the potential of natural extracts from vegetable biomass of the species Acacia melanoxylon R. Br. to obtain compounds of industrial interest, such as antioxidants and anti-inflammatory compounds, enhancing these wastes and making them economically viable. The experimental work used the hydrodistillation method with a Clevenger apparatus for the aerial parts (bark, leaves and branches) of Acacia melanoxylon species, to obtain essential oils. The residual decoction waters were evaluated for their phenolic composition. The decoction waters were evaluated for the total phenolic compounds, tannins and non-tannins by the Folin-Ciocalteau method using gallic acid as standard, expressing the results in GAE (gallic acid equivalents). The antioxidant activity was determined by the free radical DPPH • capture method and the ABTS •+ method, using Trolox as standard. Further characterization was done by fractionation of the extracts using column chromatography and thin layer chromatography. Fractions obtained were analyzed by CZE, having identified different phenolic compounds from simple to complex structures, some of which have never been reported for the genus Acacia. Finally, the anti-inflammatory activity was determined by the bovine serum albumin (BSA) inhibition method, using acetyl salicylic acid as standard. To the best of our knowledge, this is the first time that phenolic acids and flavonoids were identified in the aqueous extract of leaves/branches and bark of Acacia melanoxylon, and that the presence of O-acetylsalisylic acid is referred in Acacia species. This work allowed, not only to supplement the existing phytochemical study for this species, but also to evaluate the potential of the bioactive phenolic compounds identified, enhancing thus the valorization of biomass as an abundant source of natural compounds with pharmacological activity. It is expected to continue these lines of research for development of value added products based on these bioactive compounds useful for the food, pharmaceutical and chemical industries.