Ochuko Lucky | Federal Institute Of Industrial Research Oshodi (original) (raw)

Papers by Ochuko Lucky

SUMMARY: Soy and orange peel (C. sinensis) oils were fed to albino male rats to determine their e... more SUMMARY: Soy and orange peel (C. sinensis) oils were fed to albino male rats to determine their effects on malondialdehyde (MDA), total phospholipid (TP) content and oxidative stress biomarkers of brain tissue. Beside mouse chow, four diets were designed to contain 50% of their energy as carbohydrate, 35% as fat, and 15% as protein, and one lipid-free diet which had distilled water substituted for fat. Groups of five rats were each fed one of these diets, while a fifth group was fed pelletized mouse chow. A significant difference (p<0.05) was observed in the TP of the mouse chow group. The TP was highest (p<0.05) in those fed the soy and orange peel oil blend as compared to those fed these oils separately. Feeding soy oil led to decreased MDA in brain tissues and influenced the TP content. Significantly lower (p<0.05) GSH and SOD activities were observed in the groups fed soy oil+orange peel oil, and soy oil diets respectively. Higher significant (p<0.05) activities were observed in the orange oil fed group. Significantly higher (p<0.05) catalase activity was observed in the lipid free diet fed group, which was followed by orange peel oil, and soy oil+orange peel oil diets, respectively. A combination of both oils may be useful in the management of certain neurological diseases or illnesses and protect against other oxidative stress complications. RESUMEN: Efecto de los aceite de soja y de cáscara de naranja (Citrus sinensis) y sus mezclas sobre fosfolípi-dos totales, peroxidación lipídica y el sistema de defensa antioxidante, en tejidos cerebrales de normo ratas. Ratas albinas machos fueron alimentadas con aceites de soja y de cáscara de naranja (C. sinensis) para determinar su efecto sobre el malondialdehído (MDA), fosfolípidos (TP) y el contenido total de biomarcadores del estrés oxidativo de su tejido cerebral. Además de alimento para ratones, cuatro dietas fueron diseñadas conteniendo el 50% de la energía en forma de carbohidratos, el 35% en forma de grasa, y el 15% como proteína, y una cuarta dieta libre de lípidos donde se había sustituido la grasa por agua destilada. Grupos de cinco ratas fueron alimen-tadas cada uno con estas dietas, mientras que un quinto grupo fue alimentado con alimento para ratones pele-tizado. Se observó una diferencia significativa (p<0,05) en TP del grupo alimentado concomida para ratón. Los TP fue mayor (p<0,05) en los alimentados con mezcla de aceite de soja y de cáscaras de naranja, en comparación 2 • O.L. Erukainure et al. con los alimentados con estos aceites por separado. La alimentación con aceite de soja llevó a una disminución del MDA en los tejidos del cerebro e influyó en el contenido de TP. Se observó un descenso significativo (p<0,05) de las actividades de GSH y SOD en los grupos alimentados con aceite de soja+aceite de piel de naranja, y con las dietas de aceite de soja. Se observaron actividades significativamente más altas (p<0,05) en el grupo alimen-tado con aceite de naranja. Una actividad catalasa significativamente mas alta (p<0,05) se observó en el grupo alimentado con una dieta libre de lípidos, seguido por grupo alimentado con aceite de la cáscara de naranja y el que incluía en su dieta la mezcla aceite de soja+cáscara de naranja respectivamente. La combinación de ambos aceites puede ser útil en el control de ciertas enfermedades neurológicas y en la protección contra las complica-ciones del estrés oxidativo.

The antioxidant activities of blends of selected fruit extracts used in diabetic management was i... more The antioxidant activities of blends of selected fruit extracts used in diabetic management was investigated. Hot water extracts of unripe pawpaw fruit, guava leaf and grape fruit were formulated into drinks. Two samples (F1 and F2) were produced, with roselle calyx serving as a carrier in sample F2. Total phenolic and flavonoid contents were determined using standard procedures. The scavenging effects on hydroxyl, nitric oxide, superoxide radicals, and DPPH as well as protection against reactive oxygen species induced lipid damage in hepatic tissues were evaluated. The drinks exhibited high antioxidant activities both in vitro and ex vivo, as indicated by their ability to scavenge DPPH, hydroxyl radicals and nitric oxides, high reducing power, and protection against biological macromolecular oxidative damage. This suggests that the drinks could be protective against diabetic-induced complications. The presence of phenolic compounds
may be responsible for these effective antioxidant properties.

Hyperglyceamia-induced oxidative stress has been reported to be associated with testicular failur... more Hyperglyceamia-induced oxidative stress has been reported to be associated with testicular failure leading to
sexual dysfunction, impotence and infertility. The effect of blend of roselle calyx and selected fruits on the testicular
antioxidant activities and sperm quality of diabetic rats was investigated. Diabetes was induced by a single
intraperitoneal injection of alloxan. Treatment lasted for 14 days after induction. The rats were sacrificed by cervical
dislocation. Testicular tissues were used for assessment of GSH, catalase, SOD and lipid peroxidation. Sperm cells
were analyzed for sperm motility, counts and abnormality. Induction of diabetes led to a significant decrease in
GSH level, elevated SOD and catalase activities. These were significantly modified by the blend. The blends were
observed to reduce malondialdehyde level. Induction of diabetes led to significant decrease in the studied sperm
quality parameters, treatment with the blend significantly improved these qualities. This study indicates improved
testicular antioxidant activities and sperm qualities by single and double doses of the fruit blend suggesting its
protective potential against spermatotoxic and testicular toxicity in diabetics.