Angela Lindner | University of Florida (original) (raw)
Papers by Angela Lindner
Archives of Microbiology, 2000
Archives of Microbiology, 2005
The oxidation potential of type II groundwater methanotroph, strain CSC1, expressing soluble meth... more The oxidation potential of type II groundwater methanotroph, strain CSC1, expressing soluble methane monooxygenase, was measured in the presence of 10 ortho-substituted biphenyls with varying electronics, sterics, and hydrophobicity character for comparison with type II Methylosinus trichosporium OB3b. Strain CSC1 showed faster rates with all compounds tested, with the exception of 2-nitrobiphenyl, 2-hydroxybiphenyl, and 2-aminobiphenyl. Products of oxidation observed upon incubation of strain CSC1 with biphenyl and 2-hydroxybiphenyl were hydroxylated biphenyls that revealed less preference for the para position and different dihydroxylation positions, respectively, in comparison to those observed with M. trichosporium OB3b. Only the intramolecular hydrogen migration, or NIH-shift, product was observed in the case of 2-chlorobiphenyl, whereas M. trichosporium OB3b yielded a variety of chlorohydroxybiphenyls. Quantitative structure–biodegradation relationships constructed with the maximum observed oxygen uptake rates as a dependent variable and a variety of descriptors showed an influence of substituent electronic character on the oxidation activity of strain CSC1. However, compound hydrophobicity and not compound size, as was observed with M. trichosporium OB3b, was shown to influence rates to a greater extent. This suggests that transport of the compound through the cell membrane and to the sMMO active site is rate-determining for strain CSC1.
Environmental Toxicology and Chemistry, 2003
Indoor and Built Environment, 2010
Bioresource Technology, 2009
International Journal of Systematic and Evolutionary Microbiology, 2007
Bulletin of Environmental Contamination and Toxicology, 2005
... Toxicology Effects of Л/p/ia-Pinene and Trichloroethylene on Oxidation Potentials of Methanot... more ... Toxicology Effects of Л/p/ia-Pinene and Trichloroethylene on Oxidation Potentials of Methanotrophic Bacteria A. Pacheco, AS Lindner ... Culture Collection (ATCC) 33003) and Type II Methylosinus trichosporium OB3b (ATCC 35070), obtained from Dr. Jeremy Semrau (University ...
In a commitment to zero ozone depletion, the United Nations and the U.S. Environmental Protection... more In a commitment to zero ozone depletion, the United Nations and the U.S. Environmental Protection Agency (EPA) have called for the phase-out of the manufacture and import of hydrochlorofluorocarbons (HCFCs), used as auxiliary blowing agents (ABAs) in the manufacture of polyurethane foams. As a result, more environmentally benign alternative ABAs are being sought by the foam-blowing industry. This study examined the life cycle of HCFC-22, hydrofluorocarbon-134a (HFC-134a), and cyclopentane, which are currently used or considered as potential alternative ABAs in the manufacture of rigid polyurethane foams that serve as insulation in a model North American refrigerator. The raw material extraction/refining, manufacturing, use, and disposal stages of the life cycle of each ABA were considered, and their resulting relative impacts on ozone depletion and global warming were compared. The manufacturing, use, and disposal stages were determined to affect ozone depletion and global warming to the largest extent, emphasizing the need for a greater focus on pollution prevention opportunities in these stages. The HFC-134a life cycle yields no impact on ozone depletion and a significantly decreased global warming impact compared with its predecessor, HCFC-22, and a tradeoff of slightly higher global warming impact and fewer added safety concerns compared with its more flammable counterpart, cyclopentane.
Archives of Microbiology, 2000
Archives of Microbiology, 2005
The oxidation potential of type II groundwater methanotroph, strain CSC1, expressing soluble meth... more The oxidation potential of type II groundwater methanotroph, strain CSC1, expressing soluble methane monooxygenase, was measured in the presence of 10 ortho-substituted biphenyls with varying electronics, sterics, and hydrophobicity character for comparison with type II Methylosinus trichosporium OB3b. Strain CSC1 showed faster rates with all compounds tested, with the exception of 2-nitrobiphenyl, 2-hydroxybiphenyl, and 2-aminobiphenyl. Products of oxidation observed upon incubation of strain CSC1 with biphenyl and 2-hydroxybiphenyl were hydroxylated biphenyls that revealed less preference for the para position and different dihydroxylation positions, respectively, in comparison to those observed with M. trichosporium OB3b. Only the intramolecular hydrogen migration, or NIH-shift, product was observed in the case of 2-chlorobiphenyl, whereas M. trichosporium OB3b yielded a variety of chlorohydroxybiphenyls. Quantitative structure–biodegradation relationships constructed with the maximum observed oxygen uptake rates as a dependent variable and a variety of descriptors showed an influence of substituent electronic character on the oxidation activity of strain CSC1. However, compound hydrophobicity and not compound size, as was observed with M. trichosporium OB3b, was shown to influence rates to a greater extent. This suggests that transport of the compound through the cell membrane and to the sMMO active site is rate-determining for strain CSC1.
Environmental Toxicology and Chemistry, 2003
Indoor and Built Environment, 2010
Bioresource Technology, 2009
International Journal of Systematic and Evolutionary Microbiology, 2007
Bulletin of Environmental Contamination and Toxicology, 2005
... Toxicology Effects of Л/p/ia-Pinene and Trichloroethylene on Oxidation Potentials of Methanot... more ... Toxicology Effects of Л/p/ia-Pinene and Trichloroethylene on Oxidation Potentials of Methanotrophic Bacteria A. Pacheco, AS Lindner ... Culture Collection (ATCC) 33003) and Type II Methylosinus trichosporium OB3b (ATCC 35070), obtained from Dr. Jeremy Semrau (University ...
In a commitment to zero ozone depletion, the United Nations and the U.S. Environmental Protection... more In a commitment to zero ozone depletion, the United Nations and the U.S. Environmental Protection Agency (EPA) have called for the phase-out of the manufacture and import of hydrochlorofluorocarbons (HCFCs), used as auxiliary blowing agents (ABAs) in the manufacture of polyurethane foams. As a result, more environmentally benign alternative ABAs are being sought by the foam-blowing industry. This study examined the life cycle of HCFC-22, hydrofluorocarbon-134a (HFC-134a), and cyclopentane, which are currently used or considered as potential alternative ABAs in the manufacture of rigid polyurethane foams that serve as insulation in a model North American refrigerator. The raw material extraction/refining, manufacturing, use, and disposal stages of the life cycle of each ABA were considered, and their resulting relative impacts on ozone depletion and global warming were compared. The manufacturing, use, and disposal stages were determined to affect ozone depletion and global warming to the largest extent, emphasizing the need for a greater focus on pollution prevention opportunities in these stages. The HFC-134a life cycle yields no impact on ozone depletion and a significantly decreased global warming impact compared with its predecessor, HCFC-22, and a tradeoff of slightly higher global warming impact and fewer added safety concerns compared with its more flammable counterpart, cyclopentane.