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Papers by ASHA RANI

Research paper thumbnail of AAV-Mediated Gene Therapy for Retinal Degeneration in the rd10 Mouse Containing a Recessive PDE Mutation

Investigative Ophthalmology & Visual Science, 2008

Research paper thumbnail of Viral Vector–mediated Delivery of Estrogen Receptor-α to the Hippocampus Improves Spatial Learning in Estrogen Receptor-α Knockout Mice

Molecular Therapy, 2008

Estrogen, which influences both classical genomic and rapid membrane-associated signaling cascade... more Estrogen, which influences both classical genomic and rapid membrane-associated signaling cascades, has been implicated in the regulation of hippocampal function, including spatial learning. Gene mutation studies suggest that estrogen effects are mediated by estrogen receptor-alpha (ER-alpha); however, because gonadal steroids influence the organization of the hippocampus during development, it has been difficult to distinguish developmental effects from those specific to adults. In this study we show that lentiviral delivery of the gene encoding ER-alpha to the hippocampus of adult ER-alpha-knockout (ER-alphaKO) mice restores hippocampal responsiveness to estrogen and rescues spatial learning. We propose that constitutive estrogen receptor activity is important for maintaining hippocampus-dependent memory function in adults.

Research paper thumbnail of Gene Therapy Restores Vision-Dependent Behavior as Well as Retinal Structure and Function in a Mouse Model of RPE65 Leber Congenital Amaurosis

Research paper thumbnail of AAV-mediated gene therapy for retinal degeneration in the rd10 mouse containing a recessive PDEbeta mutation

Research paper thumbnail of Comparison of lifelong and late life exercise on oxidative stress in the cerebellum

Neurobiology of Aging, 2009

Research paper thumbnail of Influence of late-life exposure to environmental enrichment or exercise on hippocampal function and CA1 senescent physiology

Neurobiology of Aging

Aged (20–22 months) male Fischer 344 rats were randomly assigned to sedentary (A-SED), environmen... more Aged (20–22 months) male Fischer 344 rats were randomly assigned to sedentary (A-SED), environmentally-enriched (A-ENR), or exercise (A-EX) conditions. After 10–12 weeks of differential experience, the 3 groups of aged rats and young sedentary controls were tested for physical and cognitive function. Spatial discrimination learning and memory consolidation, tested on the water maze, were enhanced in environmentally-enriched compared with sedentary. A-EX exhibited improved and impaired performance on the cue and spatial task, respectively. Impaired spatial learning in A-EX was likely due to a bias in response selection associated with exercise training, as object recognition memory improved for A-EX rats. An examination of senescent hippocampal physiology revealed that enrichment and exercise reversed age-related changes in long-term depression (LTD) and long-term potentiation (LTP). Rats in the enrichment group exhibited an increase in cell excitability compared with the other 2 groups of aged animals. The results indicate that differential experience biased the selection of a spatial or a response strategy and factors common across the 2 conditions, such as increased hippocampal activity associated with locomotion, contribute to reversal of senescent synaptic plasticity.

Research paper thumbnail of Regional hippocampal differences in AKT survival signaling across the lifespan: implications for CA1 vulnerability with aging

Cell Death and Differentiation, 2009

Research paper thumbnail of 226Ra, 232Th and 40K analysis in soil samples from some areas of Punjab and Himachal Pradesh, India using gamma ray spectrometry

Radiation Measurements, 2005

Research paper thumbnail of Analysis of uranium and its correlation with some physico-chemical properties of drinking water samples from Amritsar, Punjab

Journal of Environmental Monitoring, 2003

Research paper thumbnail of ANALYSIS OF URANIUM IN DRINKING WATER SAMPLES USING LASER INDUCED FLUORIMETRY

Health Physics, 2006

Uranium concentration in drinking water samples collected from some areas of Punjab and Himachal ... more Uranium concentration in drinking water samples collected from some areas of Punjab and Himachal Pradesh has been measured using a laser induced fluorimetry technique. The sources of water comprise hand pumps and tube wells. Uranium concentration in the water samples from Punjab varies from 1.39 +/- 0.16 to 98.25 +/- 2.06 ppb with a mean value of 19.84 +/- 0.87 ppb. The uranium concentration in most of the drinking water samples from Punjab exceeds the safe limit recommended by the World Health Organization. However, the uranium concentration in water samples from Himachal Pradesh is well within the recommended levels. The annual effective dose equivalent associated with drinking water due to uranium concentration is estimated from its annual intake using dosimetric information based on ICRP Report 72. The resulting value of the annual effective dose from drinking water sources is in the range of 0.13 to 81.59 muSv. The annual effective dose received by the population due to the consumption of drinking water from these areas is well within the recommended limit. In order to check the accuracy of the technique a few water samples were also analyzed using a fission track registration technique. A good agreement has been observed between the uranium values determined by these techniques.

Research paper thumbnail of Natural radioactivity levels in soil samples from some areas of Himachal Pradesh, India using γ-ray spectrometry

Atmospheric Environment, 2005

Research paper thumbnail of Kamaljeet singh

Research paper thumbnail of AAV-Mediated Gene Therapy for Retinal Degeneration in the rd10 Mouse Containing a Recessive PDE Mutation

Investigative Ophthalmology & Visual Science, 2008

Research paper thumbnail of Viral Vector–mediated Delivery of Estrogen Receptor-α to the Hippocampus Improves Spatial Learning in Estrogen Receptor-α Knockout Mice

Molecular Therapy, 2008

Estrogen, which influences both classical genomic and rapid membrane-associated signaling cascade... more Estrogen, which influences both classical genomic and rapid membrane-associated signaling cascades, has been implicated in the regulation of hippocampal function, including spatial learning. Gene mutation studies suggest that estrogen effects are mediated by estrogen receptor-alpha (ER-alpha); however, because gonadal steroids influence the organization of the hippocampus during development, it has been difficult to distinguish developmental effects from those specific to adults. In this study we show that lentiviral delivery of the gene encoding ER-alpha to the hippocampus of adult ER-alpha-knockout (ER-alphaKO) mice restores hippocampal responsiveness to estrogen and rescues spatial learning. We propose that constitutive estrogen receptor activity is important for maintaining hippocampus-dependent memory function in adults.

Research paper thumbnail of Gene Therapy Restores Vision-Dependent Behavior as Well as Retinal Structure and Function in a Mouse Model of RPE65 Leber Congenital Amaurosis

Research paper thumbnail of AAV-mediated gene therapy for retinal degeneration in the rd10 mouse containing a recessive PDEbeta mutation

Research paper thumbnail of Comparison of lifelong and late life exercise on oxidative stress in the cerebellum

Neurobiology of Aging, 2009

Research paper thumbnail of Influence of late-life exposure to environmental enrichment or exercise on hippocampal function and CA1 senescent physiology

Neurobiology of Aging

Aged (20–22 months) male Fischer 344 rats were randomly assigned to sedentary (A-SED), environmen... more Aged (20–22 months) male Fischer 344 rats were randomly assigned to sedentary (A-SED), environmentally-enriched (A-ENR), or exercise (A-EX) conditions. After 10–12 weeks of differential experience, the 3 groups of aged rats and young sedentary controls were tested for physical and cognitive function. Spatial discrimination learning and memory consolidation, tested on the water maze, were enhanced in environmentally-enriched compared with sedentary. A-EX exhibited improved and impaired performance on the cue and spatial task, respectively. Impaired spatial learning in A-EX was likely due to a bias in response selection associated with exercise training, as object recognition memory improved for A-EX rats. An examination of senescent hippocampal physiology revealed that enrichment and exercise reversed age-related changes in long-term depression (LTD) and long-term potentiation (LTP). Rats in the enrichment group exhibited an increase in cell excitability compared with the other 2 groups of aged animals. The results indicate that differential experience biased the selection of a spatial or a response strategy and factors common across the 2 conditions, such as increased hippocampal activity associated with locomotion, contribute to reversal of senescent synaptic plasticity.

Research paper thumbnail of Regional hippocampal differences in AKT survival signaling across the lifespan: implications for CA1 vulnerability with aging

Cell Death and Differentiation, 2009

Research paper thumbnail of 226Ra, 232Th and 40K analysis in soil samples from some areas of Punjab and Himachal Pradesh, India using gamma ray spectrometry

Radiation Measurements, 2005

Research paper thumbnail of Analysis of uranium and its correlation with some physico-chemical properties of drinking water samples from Amritsar, Punjab

Journal of Environmental Monitoring, 2003

Research paper thumbnail of ANALYSIS OF URANIUM IN DRINKING WATER SAMPLES USING LASER INDUCED FLUORIMETRY

Health Physics, 2006

Uranium concentration in drinking water samples collected from some areas of Punjab and Himachal ... more Uranium concentration in drinking water samples collected from some areas of Punjab and Himachal Pradesh has been measured using a laser induced fluorimetry technique. The sources of water comprise hand pumps and tube wells. Uranium concentration in the water samples from Punjab varies from 1.39 +/- 0.16 to 98.25 +/- 2.06 ppb with a mean value of 19.84 +/- 0.87 ppb. The uranium concentration in most of the drinking water samples from Punjab exceeds the safe limit recommended by the World Health Organization. However, the uranium concentration in water samples from Himachal Pradesh is well within the recommended levels. The annual effective dose equivalent associated with drinking water due to uranium concentration is estimated from its annual intake using dosimetric information based on ICRP Report 72. The resulting value of the annual effective dose from drinking water sources is in the range of 0.13 to 81.59 muSv. The annual effective dose received by the population due to the consumption of drinking water from these areas is well within the recommended limit. In order to check the accuracy of the technique a few water samples were also analyzed using a fission track registration technique. A good agreement has been observed between the uranium values determined by these techniques.

Research paper thumbnail of Natural radioactivity levels in soil samples from some areas of Himachal Pradesh, India using γ-ray spectrometry

Atmospheric Environment, 2005

Research paper thumbnail of Kamaljeet singh

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