L. Slim | Faculté De Pharmacie De Monastir (original) (raw)
Papers by L. Slim
Médecine et Maladies Infectieuses, 2015
We aimed to evaluate the GeneXpert(®) MTB/RIF test for the diagnosis of extrapulmonary tuberculos... more We aimed to evaluate the GeneXpert(®) MTB/RIF test for the diagnosis of extrapulmonary tuberculosis. The test simultaneously detects Mycobacterium tuberculosis complex and resistance to rifampicin. We analyzed 153 clinical samples collected in a tertiary hospital in Sfax, Tunisia, between 2013 and 2014. We performed the GeneXpert(®) test, a Ziehl-Neelsen and auramine-rhodamine staining, conventional culture on MGIT 960 and LJ media, and we tested the resistance to anti-tuberculosis drugs on MGIT 960 and LJ media for each sample. Diagnosis was based on clinical, radiological, microbiological, pathological, and therapeutic data. We considered that 59 patients out of 153 presented with tuberculosis. PCR was positive in 50 samples and all of these samples were susceptible to rifampicin. Sensitivity, specificity, positive predictive value, and negative predictive value of the GeneXpert(®) test were 84.7%, 96.8%, 94.3%, and 91%, respectively, compared with diagnosis. We observed a statistically significant difference between the direct test and the GeneXpert(®) test, and between culture and the GeneXpert(®) test. No statistically significant difference was observed between pathological results and the GeneXpert(®) test. Sensitivity of the GeneXpert(®) test was 87.5% in biopsies, 80% in pus and abscesses, and 66.7% in biological fluids. All strains were susceptible to rifampicin with culture and GeneXpert(®) test. The GeneXpert(®) test helped detect a higher proportion of M. tuberculosis complex. It does not replace conventional diagnostic methods but it is a useful addition to achieve better sensitivity and obtain rapid results.
International Journal of Mycobacteriology, 2015
Presse médicale (Paris, France : 1983), Jan 18, 1993
La Tunisie médicale, 1989
Médecine et Maladies Infectieuses, 1997
Journal of Clinical Microbiology, 2002
Twelve 16S-23S ribosomal DNA intergenic spacer (ITS-PCR) types were identified among 57 Staphyloc... more Twelve 16S-23S ribosomal DNA intergenic spacer (ITS-PCR) types were identified among 57 Staphylococcus intermedius isolates from humans and other animals. Six ITS-PCR types were host specific, and most human and canine strains belonged to the same types (A and J). Pigeon, horse, and mink strains appeared more heterogeneous.
Revue des Maladies Respiratoires, 2014
Revue des Maladies Respiratoires, 2014
Revue des Maladies Respiratoires, 2012
Revue des Maladies Respiratoires, 2007
Revue des Maladies Respiratoires, 2015
Revue des Maladies Respiratoires, 2004
Revue des Maladies Respiratoires, 2014
Revue des Maladies Respiratoires, 2015
Revue des Maladies Respiratoires, 2006
La Tunisie médicale, 1996
Médecine et Maladies Infectieuses, 2015
We aimed to evaluate the GeneXpert(®) MTB/RIF test for the diagnosis of extrapulmonary tuberculos... more We aimed to evaluate the GeneXpert(®) MTB/RIF test for the diagnosis of extrapulmonary tuberculosis. The test simultaneously detects Mycobacterium tuberculosis complex and resistance to rifampicin. We analyzed 153 clinical samples collected in a tertiary hospital in Sfax, Tunisia, between 2013 and 2014. We performed the GeneXpert(®) test, a Ziehl-Neelsen and auramine-rhodamine staining, conventional culture on MGIT 960 and LJ media, and we tested the resistance to anti-tuberculosis drugs on MGIT 960 and LJ media for each sample. Diagnosis was based on clinical, radiological, microbiological, pathological, and therapeutic data. We considered that 59 patients out of 153 presented with tuberculosis. PCR was positive in 50 samples and all of these samples were susceptible to rifampicin. Sensitivity, specificity, positive predictive value, and negative predictive value of the GeneXpert(®) test were 84.7%, 96.8%, 94.3%, and 91%, respectively, compared with diagnosis. We observed a statistically significant difference between the direct test and the GeneXpert(®) test, and between culture and the GeneXpert(®) test. No statistically significant difference was observed between pathological results and the GeneXpert(®) test. Sensitivity of the GeneXpert(®) test was 87.5% in biopsies, 80% in pus and abscesses, and 66.7% in biological fluids. All strains were susceptible to rifampicin with culture and GeneXpert(®) test. The GeneXpert(®) test helped detect a higher proportion of M. tuberculosis complex. It does not replace conventional diagnostic methods but it is a useful addition to achieve better sensitivity and obtain rapid results.
International Journal of Mycobacteriology, 2015
Presse médicale (Paris, France : 1983), Jan 18, 1993
La Tunisie médicale, 1989
Médecine et Maladies Infectieuses, 1997
Journal of Clinical Microbiology, 2002
Twelve 16S-23S ribosomal DNA intergenic spacer (ITS-PCR) types were identified among 57 Staphyloc... more Twelve 16S-23S ribosomal DNA intergenic spacer (ITS-PCR) types were identified among 57 Staphylococcus intermedius isolates from humans and other animals. Six ITS-PCR types were host specific, and most human and canine strains belonged to the same types (A and J). Pigeon, horse, and mink strains appeared more heterogeneous.
Revue des Maladies Respiratoires, 2014
Revue des Maladies Respiratoires, 2014
Revue des Maladies Respiratoires, 2012
Revue des Maladies Respiratoires, 2007
Revue des Maladies Respiratoires, 2015
Revue des Maladies Respiratoires, 2004
Revue des Maladies Respiratoires, 2014
Revue des Maladies Respiratoires, 2015
Revue des Maladies Respiratoires, 2006
La Tunisie médicale, 1996