Retromer-Vps5: membrane tubules, tomogram 7. (original) (raw)

2018-10-10 REL PDBe PDBe PDBe 2018-07-31 2018-09-05 2018-09-26 2018-10-10 Retromer-Vps5: membrane tubules, tomogram 7. Kovtun O Leneva N Ariotti N Teasdale RD Owen DJ Collins BM Briggs JAG Kovtun O Leneva N Bykov YS Ariotti N Teasdale RD Schaffer M Engel BD Owen DJ Briggs JAG Collins BM Structure of the membrane-assembled retromer coat determined by cryo-electron tomography. Nature UK 561 561 564 2018 30224749 doi:10.1038/s41586-018-0526-z 1476-4687 0006 NATUAS EMD-0162 associated EM volume Retromer-Vps5 complex assembled on the membrane. Retromer-Vps5 complex assembled on the membrane. 0 1 2 Vps26/Vps35/Vps29 trimer recruited to the membrane via Vps5 PX-BAR protein. Chaetomium thermophilum Escherichia coli tomography threeDArray 1.1 7.5 20.0 C8H19KN2O5S HEPES-KOH 200.0 NaCl sodium chloride ETHANE 95 292 LEICA EM GP The solution-assembled complex was incubated with Folch liposomes at room temperature. BBI solutions 10 NO SECTIONING FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 70.0 2.7 105000. FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GIF Quantum LS 20 GATAN K2 QUANTUM (4k x 4k) SUPER-RESOLUTION 1 3.17 Tomographic tilt series were acquired with the dose-symmetric tilt-scheme (Hagen et al., J Struct Biol. 2017) 1 After motion correction in "alignframes" (IMOD), each of the images in the tilt series was low-pass filtered according to the electron-dose acquired by the sample (Grant and Grigorieff, 2015). BACK PROJECTION IMOD 41 emd_0162.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 928928 225 00 47 928 928 225 5011.2 5011.2 1215.0 90.0 90.0 90.0 X Y Z -1626.608799999999974 1465.605499999999893 112.031409999999994 204.947020000000009 5.4 5.4 5.4 AUTHOR ::::EMDATABANK.org::::EMD-0162:::: Retromer-Vps5 membrane tubules, tomogram 7.