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Research paper thumbnail of Tsukamurella strandjordae sp. nov., a Proposed New Species Causing Sepsis

Journal of Clinical Microbiology, 2001

We have isolated a gram-positive, weakly acid-alcohol-fast, irregular rod-shaped bacterium from c... more We have isolated a gram-positive, weakly acid-alcohol-fast, irregular rod-shaped bacterium from cultures of blood from a 5-year-old girl with acute myelogenous leukemia. This isolate was compared with 14 other strains including reference strains of Tsukamurella species by a polyphasic approach based on physiological and biochemical properties, whole-cell short-chain fatty acid and mycolic acid analyses, DNA-DNA hybridization, and sequencing of the 16S rRNA gene. This isolate represents a new taxon within the genus Tsukamurella for which we propose the name Tsukamurella strandjordae sp. nov. Our study also revealed that Tsukamurella paurometabola ATCC 25938 represents a misnamed Tsukamurella inchonensis isolate and confirms that Tsukamurella wratislaviensis belongs to the genus Rhodococcus.

Research paper thumbnail of Characterization of a Novel Group of Mycobacteria and Proposal of Mycobacterium sherrisii sp. nov

We describe here the characterization of five isolates of Mycobacterium simiae-like organisms rep... more We describe here the characterization of five isolates of Mycobacterium simiae-like organisms representing a novel group based on whole-cell fatty acid analysis and genotypic evaluation. Two of the five isolates in this study, W55 and W58, were previously considered to belong to M. simiae serotype 2. Analysis of cellular fatty acids by gas-liquid chromatography indicated a close clustering of this group, which was well differentiated from the other M. simiae-like species. Molecular characterization was performed by nucleic acid sequencing of the small subunit rRNA gene and the gene encoding the 65-kDa heat shock protein and genomic DNA hybridization. Sequence analysis of the entire 16S rRNA gene showed a unique sequence most closely related to those of M. triplex and M. simiae. The hsp65 partial gene sequence was identical for the five isolates, with 97% identity to the M. simiae type strain. However, qualitative whole genomic DNA hybridization analysis confirmed that this group is genetically distinct from M. simiae and M. triplex. Antimicrobial susceptibilities for this group resemble those of M. simiae and M. lentiflavum. We conclude that this group represents a unique Mycobacterium species for which we propose the name Mycobacterium sherrisii sp. nov.

Research paper thumbnail of A 4GHz 300mW 64b integer execution ALU with dual supply voltages in 90nm CMOS

Fast 32b and 64b ALU operations with single-cycle latency and throughput are essential ingredient... more Fast 32b and 64b ALU operations with single-cycle latency and throughput are essential ingredients of high-performance super-scalar microprocessor execution cores. In a typical ALU operation, the lower order 32b of the ALU output are required early for address ...

Research paper thumbnail of A 4GHz 300mW 64-bit integer execution ALU with dual supply voltages in 90-nm CMOS

IEEE Journal of Solid-state Circuits, 2005

IEEE JOURNAL OF SOLID-STATE CIRCUITS, VOL. 40, NO. 1, JANUARY 2005 ... A 4-GHz 300-mW 64-bit Inte... more IEEE JOURNAL OF SOLID-STATE CIRCUITS, VOL. 40, NO. 1, JANUARY 2005 ... A 4-GHz 300-mW 64-bit Integer Execution ALU ... With Dual Supply Voltages in 90-nm CMOS ... Sanu K. Mathew, Member, IEEE, Mark A. Anders, Member, IEEE, Brad Bloechel, Member, IEEE, ...

Research paper thumbnail of CC Chemokine Receptor (CCR)3/Eotaxin Is Followed by CCR4/Monocyte-derived Chemokine in Mediating Pulmonary T Helper Lymphocyte Type 2 Recruitment after Serial Antigen Challenge In Vivo

Isolated peripheral blood CD4 cells from allergic individuals express CC chemokine receptor (CCR)... more Isolated peripheral blood CD4 cells from allergic individuals express CC chemokine receptor (CCR)3 and CCR4 after expansion in vitro. In addition, human T helper type 2 (Th2) cells polarized in vitro selectively express CCR3 and CCR4 at certain stages of activation/differentiation and respond preferentially to the ligands eotaxin and monocyte-derived chemokine (MDC). However, controversy arises when the in vivo significance of this distinct expression is discussed. To address the functional role of CCR3/eotaxin and CCR4/MDC during the in vivo recruitment of Th2 cells, we have transferred effector Th cells into naive mice to induce allergic airway disease. Tracking of these cells after repeated antigen challenge has established that both CCR3/ eotaxin and CCR4/MDC axes contribute to the recruitment of Th2 cells to the lung, demonstrating the in vivo relevance of the expression of these receptors on Th2 cells. We have shown that involvement of the CCR3/eotaxin pathway is confined to early stages of the response in vivo, whereas repeated antigen stimulation results in the predominant use of the CCR4/MDC pathway. We propose that effector Th2 cells respond to both CCR3/eotaxin and CCR4/MDC pathways initially, but that a progressive increase in CCR4-positive cells results in the predominance of the CCR4/MDC axis in the long-term recruitment of Th2 cells in vivo.

Research paper thumbnail of The CD28Related Molecule ICOS Is Required for Effective T Cell–Dependent Immune Responses

Research paper thumbnail of Resolution of Bronchial Hyperresponsiveness and Pulmonary Inflammation Is Associated with IL3 and Tissue Leukocyte Apoptosis

Research paper thumbnail of Membership Application form Your full name Your university

Research paper thumbnail of Tsukamurella strandjordae sp. nov., a Proposed New Species Causing Sepsis

Journal of Clinical Microbiology, 2001

We have isolated a gram-positive, weakly acid-alcohol-fast, irregular rod-shaped bacterium from c... more We have isolated a gram-positive, weakly acid-alcohol-fast, irregular rod-shaped bacterium from cultures of blood from a 5-year-old girl with acute myelogenous leukemia. This isolate was compared with 14 other strains including reference strains of Tsukamurella species by a polyphasic approach based on physiological and biochemical properties, whole-cell short-chain fatty acid and mycolic acid analyses, DNA-DNA hybridization, and sequencing of the 16S rRNA gene. This isolate represents a new taxon within the genus Tsukamurella for which we propose the name Tsukamurella strandjordae sp. nov. Our study also revealed that Tsukamurella paurometabola ATCC 25938 represents a misnamed Tsukamurella inchonensis isolate and confirms that Tsukamurella wratislaviensis belongs to the genus Rhodococcus.

Research paper thumbnail of Characterization of a Novel Group of Mycobacteria and Proposal of Mycobacterium sherrisii sp. nov

We describe here the characterization of five isolates of Mycobacterium simiae-like organisms rep... more We describe here the characterization of five isolates of Mycobacterium simiae-like organisms representing a novel group based on whole-cell fatty acid analysis and genotypic evaluation. Two of the five isolates in this study, W55 and W58, were previously considered to belong to M. simiae serotype 2. Analysis of cellular fatty acids by gas-liquid chromatography indicated a close clustering of this group, which was well differentiated from the other M. simiae-like species. Molecular characterization was performed by nucleic acid sequencing of the small subunit rRNA gene and the gene encoding the 65-kDa heat shock protein and genomic DNA hybridization. Sequence analysis of the entire 16S rRNA gene showed a unique sequence most closely related to those of M. triplex and M. simiae. The hsp65 partial gene sequence was identical for the five isolates, with 97% identity to the M. simiae type strain. However, qualitative whole genomic DNA hybridization analysis confirmed that this group is genetically distinct from M. simiae and M. triplex. Antimicrobial susceptibilities for this group resemble those of M. simiae and M. lentiflavum. We conclude that this group represents a unique Mycobacterium species for which we propose the name Mycobacterium sherrisii sp. nov.

Research paper thumbnail of A 4GHz 300mW 64b integer execution ALU with dual supply voltages in 90nm CMOS

Fast 32b and 64b ALU operations with single-cycle latency and throughput are essential ingredient... more Fast 32b and 64b ALU operations with single-cycle latency and throughput are essential ingredients of high-performance super-scalar microprocessor execution cores. In a typical ALU operation, the lower order 32b of the ALU output are required early for address ...

Research paper thumbnail of A 4GHz 300mW 64-bit integer execution ALU with dual supply voltages in 90-nm CMOS

IEEE Journal of Solid-state Circuits, 2005

IEEE JOURNAL OF SOLID-STATE CIRCUITS, VOL. 40, NO. 1, JANUARY 2005 ... A 4-GHz 300-mW 64-bit Inte... more IEEE JOURNAL OF SOLID-STATE CIRCUITS, VOL. 40, NO. 1, JANUARY 2005 ... A 4-GHz 300-mW 64-bit Integer Execution ALU ... With Dual Supply Voltages in 90-nm CMOS ... Sanu K. Mathew, Member, IEEE, Mark A. Anders, Member, IEEE, Brad Bloechel, Member, IEEE, ...

Research paper thumbnail of CC Chemokine Receptor (CCR)3/Eotaxin Is Followed by CCR4/Monocyte-derived Chemokine in Mediating Pulmonary T Helper Lymphocyte Type 2 Recruitment after Serial Antigen Challenge In Vivo

Isolated peripheral blood CD4 cells from allergic individuals express CC chemokine receptor (CCR)... more Isolated peripheral blood CD4 cells from allergic individuals express CC chemokine receptor (CCR)3 and CCR4 after expansion in vitro. In addition, human T helper type 2 (Th2) cells polarized in vitro selectively express CCR3 and CCR4 at certain stages of activation/differentiation and respond preferentially to the ligands eotaxin and monocyte-derived chemokine (MDC). However, controversy arises when the in vivo significance of this distinct expression is discussed. To address the functional role of CCR3/eotaxin and CCR4/MDC during the in vivo recruitment of Th2 cells, we have transferred effector Th cells into naive mice to induce allergic airway disease. Tracking of these cells after repeated antigen challenge has established that both CCR3/ eotaxin and CCR4/MDC axes contribute to the recruitment of Th2 cells to the lung, demonstrating the in vivo relevance of the expression of these receptors on Th2 cells. We have shown that involvement of the CCR3/eotaxin pathway is confined to early stages of the response in vivo, whereas repeated antigen stimulation results in the predominant use of the CCR4/MDC pathway. We propose that effector Th2 cells respond to both CCR3/eotaxin and CCR4/MDC pathways initially, but that a progressive increase in CCR4-positive cells results in the predominance of the CCR4/MDC axis in the long-term recruitment of Th2 cells in vivo.

Research paper thumbnail of The CD28Related Molecule ICOS Is Required for Effective T Cell–Dependent Immune Responses

Research paper thumbnail of Resolution of Bronchial Hyperresponsiveness and Pulmonary Inflammation Is Associated with IL3 and Tissue Leukocyte Apoptosis

Research paper thumbnail of Membership Application form Your full name Your university

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