İbrahim KiliÇ | University of Gaziantep (original) (raw)

Papers by İbrahim KiliÇ

DergiPark (Istanbul University), Dec 31, 2020

A commercial strain of Candida albicans10231 was grown and isolated using Sabouraud's Dextrose Ag... more A commercial strain of Candida albicans10231 was grown and isolated using Sabouraud's Dextrose Agar Media. The samples were then subjected to lyophilization before protein extraction. TCA/acetone/methanol/phenol method has been used for protein extraction. For estimation of the percentage of extracted protein, BCA Protein Macro Assay Kit was applied. The samples were then transferred to the spectrophotometer for reading at wave length of 562 nm. The results show that the profile of the quantity of protein concentrations obtained upon supplementation of the following chemicals: PBS, ammonium bicarbonate, and 50mM of SDS buffer, were 0.133mg/mL, 0.080mg/mL, and 0.068mg/mL respectively.

International Journal of Pharmacology, Aug 1, 2016

The American Journal of Chinese Medicine, 2007

The aim of this study is to investigate the antitumor activity of Plantago major L. extract in Eh... more The aim of this study is to investigate the antitumor activity of Plantago major L. extract in Ehrlich ascites tumor (EAT) bearing Balb/C mice in vivo. Thirty male Balb/C mice were divided into 5 groups: 3 treatment groups and 2 control groups (6 per group). Treatment groups and the negative control group were injected with EAT (1 x 10(6) cells) intraperitoneally to develop ascites tumor. P. major L. extract (1%, 2% and 3% concentration extracts, 0.1 ml/day/mouse) were given p.o. for 10 alternate days. The control group was treated with 0.9% NaCl solution (0.1 ml/day/mouse). The changes of body weight in animals were recorded. On the 11th day, all of the mice were sacrified and their tissues were stained with haematoxylen and eosin for pathological studies. Body weights of in 3 treatment groups and the negative control group were elevated because of tumor burden. The maximal weight gain was recorded in the negative control group and the minimal weight gain was recorded in Group I. Pathological studies showed that P. major L. extract (especially 1% concentration) has inhibitive effect on EAT. P. major has an inhibitory effect on EAT in a dose dependent manner.

DergiPark (Istanbul University), Dec 31, 2020

Allergy is an important health problem affecting public health. Allergic diseases occur when the ... more Allergy is an important health problem affecting public health. Allergic diseases occur when the immune system reacts to non-harmful substances with the effect of genetic predisposition and environmental factors. According to the data of the World Allergy Organization (WAO), the prevalence of allergies in different countries varies between 10-40%. Pollen, mold, animal hair, house dust mite, medicines, and foods are the most common allergen agents. Common mushrooms in nature have the potential to produce allergenic proteins. Penicillium, Aspergillus, Rhizopus, and Mucor species, which are allergic fungi, are widely found in nature. In our country, 614 patients with respiratory allergy have been reported to develop allergic reactions against Aspergillus fumigatus, Trichophyton rubrum, Mucor, Penicillium notatum, Aspergillus niger, and Alternaria tenius. In recent years, the cases of allergies caused by molds have increased significantly and studies to determine the causing allergens have accelerated. Mucor mucedo (brown bread mold) was used in our study. Mucor mucedo produced in our laboratory was collected and allergen fungus protein was extracted by 2 different extraction methods. By preparing protein samples from prepared mushroom extracts, the total concentration of potential allergen proteins was determined by the BCA method. According to the data obtained, it was determined that the protein concentration of the mushroom samples dried by that were subjected to dialysis was higher than ethanol. As a result, Mucor mucedo was found to have a high protein concentration and revealed basic data for further analysis.

International Journal of Applied Research in Natural Products, Dec 14, 2012

This study was designed to address whether simultaneous primary chemo-hormonal therapy provides a... more This study was designed to address whether simultaneous primary chemo-hormonal therapy provides additional activity compared with chemotherapy alone in breast cancer patients with operable or locally advanced disease. Between January 1997 and January 2002, 211 consecutive patients with T2-4, N0-1, M0 breast cancer were randomized to receive either epirubicin alone (EPI) or epirubicin plus tamoxifen (EPI-TAM). Ki67 expression was evaluated immunohistochemically in tumor specimens obtained before chemotherapy by incision biopsy and at definitive surgery. Tumor shrinkage of >50% was obtained in 76% of patients randomized in the EPI arm and 81.9% of patients randomized in the EPI-TAM arm (not significant). The corresponding rates of clinical and pathological complete response were 20.2 and 21.9% (not significant), and 4.8 and 6.7% (not significant), respectively. Pathologically complete response was more frequently observed in estrogen receptor (ER)-negative (ER-) tumors (P=0.04) and correlated with elevated baseline Ki67 expression (P<0.01). Both EPI and EPI-TAM treatments resulted in a significant reduction in Ki67 expression, either in overall patients (P=0.000) or in patients with ER+ breast cancer (P=0.000). The reduction in Ki67 immunostaining in the EPI-TAM arm was greater than in the EPI arm, leading to a lower Ki67 expression at post-operative residual histology (P=0.0041). The addition of tamoxifen to epirubicin chemotherapy did not improve the response rate but led to a significantly higher reduction in the Ki67 expression. Baseline elevated Ki67 expression and the ERstatus were both associated with a greater chance of obtaining a pathological complete response at residual histology.

Plant Pathology Journal, Dec 15, 2006

Dünya'da alternatif tedavilerde, bitkisel ekstreler birçok hastalıkta tedavi amaçlı kullanılmakta... more Dünya'da alternatif tedavilerde, bitkisel ekstreler birçok hastalıkta tedavi amaçlı kullanılmaktadır. Ülkemizin farklı iklim ve ekolojik koşullara sahip olması, bulunan floranın sayısal açıdan yüksek oranda bitki türü ve çeşitliliği barındırmasından dolayı ülkemizde medikal amaçlı tüketimi yapılan çok sayıda bitki türü vardır. Bu bitkilerin çoğunun antimikrobiyal etkilerinin varlığı hem yurtdışında hem de ülkemizde yapılan araştırmalar sonucunda bildirilmiştir. Doğada kendiliğinden yetişen bazı bitki ekstrelerinin ve uçucu yağlarının bakterilere olduğu kadar, mantarlara karşı da antifungal aktivite gösterdiği yapılan çalışmalarda tespit edilmiştir. Bu çalışmada çıkarılan özütlerin Stenotrophomonas maltophilia bakterisine karşı antimiktorbiyal etkileri ve DNA koruyucu potansiyeli çalışılmıştır. Antimikrobiyal aktivite disk difüzyon yöntemi ile çalışılmıştır. DNA koruyucu aktivite pBR322 plazmid DNA'sı ile H2O2 ve UV-C kullanılarak çalışılmıştır. Su ve Methanol özütlerinin Stenotrophomonas maltophilia üzerine etkili olduğu saptanmıştır. Diğer yandan DNA koruyucu aktivite bakımından oldukça yüksek koruyucu aktivite gösterdiği çalışma kapsamında belirlenmiştir.

The Eurasia Proceedings of Science Technology Engineering and Mathematics, Aug 19, 2018

Allergic diseases are a major public health problem in the modern societies. Pollens dispersed th... more Allergic diseases are a major public health problem in the modern societies. Pollens dispersed through wind are one of the major aeroallergens. When they are released to atmosphere in sufficient amount, they can cause the development of diseases such as asthma, rhinitis, rhinoconjunctivitis etc. in allergically hypersensitive individuals. The spreading of pollens are influenced by their amount in the air, their structures, geographic areas and the climate. Therefore, the types of pollens that hypersensitive individuals who live in different regions are exposed to may differ and different allergic reactions may occur in affected individuals. The high amount of allergens in pollens enhance the sensitivity to pollens. Thus the research in region-specific plant species allergenic effects is very important. Pollens of linden (Tilia cordata), Anatolian oak (Quercus ithaburensis) and birch (Betula alba) are important allergen sources in Gaziantep province. Pollen allergens are water soluble, stable proteins or glycoproteins of molecular weight between 5-80 kD. A single pollen type usually contains several different allergens. Pollens from linden (T. cordata), Anatolian oak (Q. ithaburensis) and birch (B. alba) were collected during pollination period and their extracts were prepared. For identification of pollens morphology slides were prepared according to Wodehouse's method and images were taken under light microscope. Total concentrations of potential allergen proteins were determined from prepared pollen extracts using the BCA method. In this study, we aimed to prepare extracts of pollens from linden, Anatolian oak and birch widely grown in wooded areas of Gaziantep University for the study of allergens and their use for diagnosing allergic diseases.

The Eurasia Proceedings of Science Technology Engineering and Mathematics, Aug 19, 2018

Allergens can be defined as antigens that stimulate specific IgE (Immunoglobulin E) antibody form... more Allergens can be defined as antigens that stimulate specific IgE (Immunoglobulin E) antibody formation and react with it. In this study, the morphologies of Pistachia vera allergenic pollens belonging to Anacardiaceae family in South Eastern Anatolia were examined by light microscopy and pollens extracts were prepared from these species. The specimens used in morphological studies were obtained from Gaziantep University campus area. Slides of pollens were prepared according to Wodehouse (1935) method and the morphological characteristics of pollens were determined using microphotographs taken by light microscopy. Pollens crude extracts were obtaıned from the flora grown in this region. Extraction method established by Aytug et al. (1991) was used to prepare crude extracts from the collected pollens, 'Coca' solution was added extensively and sterile filtration technique was used for sterilization. BCA assay and absorbance measurements were used to determine the protein concentration. Standard curve was generated using concentrations and absorbance values of standard protein samples prepared at different concentrations (0, 1-1mg/ml). The protein concentration of the pollens extracts was calculated using the correct equation obtained from this curve. These crude extracts contain many major and minor allergens that can be used to diagnose allergic diseases. Due to the very high concentration of allergenic proteins in P. vera, it can be included in allergen kits. Moreover, allergy tests should be conducted to those living in this region. The results obtained from this study can both contribute to systematic studies and to the treatment of allergic diseases caused by these types of pollens.

African Journal of Biotechnology, Jun 27, 2011

Stethoscopes are one of the most commonly used medical devices and have been reported to be poten... more Stethoscopes are one of the most commonly used medical devices and have been reported to be potential sources of hospital acquired infections. In this study, we aimed to find out the bacterial contamination of stethoscopes used by health-care staff. Swab samples were taken from the surface of the diaphragm of the stethoscopes used by health personnel in four hospitals including three secondline and one third-line health care institution in Turkey. The samples were inoculated onto bacteriological and mycological media. For identification of the microorganisms, conventional methods and Vitek2 (Biomérieux) were performed. Swab samples were taken from 121 stethoscopes. We found bacterial and fungal contamination on 92 (76%) of the stethoscopes. 15 out 90 (16.3%) had potential pathogens including methicillin susceptible Staphylococcus aureus (5), methicillin resistance Staphylococcus aureus (4), Escherichia coli (3), Acinetobacter baumannii, Acinetobacter haemolyticus and Enterococcus spp. Of the 121 health-care persons, only 61 regularly cleaned their stethoscopes by various disinfectants. The statistical difference between the two groups in terms of pathogen and microorganism isolation was not determined (p>0.05). Although stethoscopes are uncritical medical devices, they could contain pathogen microorganisms and they might be a potential source of hospital acquired infections.

Phosphorus Sulfur and Silicon and The Related Elements, Oct 11, 2013

Abstract The synthesis and characterization of 18 novel thiosemicarbazones have been investigated... more Abstract The synthesis and characterization of 18 novel thiosemicarbazones have been investigated as part of a research program on development of compounds with antibacterial and antioxidant activities. Among the tested compounds, 2-(4-hydroxybenzylidene)-N-[4-(trifluoromethoxy)phenyl]hydrazine carbothioamide (3g) and 2-(thiophen-2-ylmethylidene)-N-[4-(morpholin-4-yl)phenyl]hydrazine carbothioamide (4b) showed excellent inhibition potency at low concentration (0.5 μg/mL) against Gram-positive pathogens (Enterococcus faecalis and Staphylococcus aureus). All tested compounds were also found to possess antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation. [Supplementary materials are available for this article. Go to the publisher's online edition of Phosphorus, Sulfur, and Silicon and the Related Elements for the following free supplemental files: Additional text, tables, and figures.] GRAPHICAL ABSTRACT

Journal of chemistry and nutritional biochemistry, Dec 30, 2022

Background: In this study, it was aimed to test the anti-tumoral effects of melatonin in vivo. In... more Background: In this study, it was aimed to test the anti-tumoral effects of melatonin in vivo. In the literature review, no study was found that examined the anti-tumoral effect of melatonin at night on an experimental tumor model in vivo. Therefore, in the study, the anti-tumoral effect of melatonin was tested in solid tumor tissue formed in Swiss albino male mice. Methods: Swiss albino male mice formed Swiss albino male mice in vivo and 80 Swiss albino male mice weighing 25-30 g and aged 10-12 weeks were used. The subjects were divided into 10 groups in total (n=8). These groups are divided into two main groups as light and dark. All animals except the control group were injected intramuscularly (i.m.) with EAT to create EST in the right leg scapula of the subjects. Different doses of melatonin were injected intraperitoneally (i.p.) daily to the experimental groups. EST was created by inoculating EAT 2 days after administration of melatonin. Melatonin application was made between 18:00 and 20:00 in the evening. In addition, light groups were exposed to 580 µW fluorescent light between 02:00 and 04:00 at night. Daily food and water consumption and weight gains of all animals were followed and recorded, and the application was continued for 14 days. Cardiac blood was collected from all animals with heparinized syringes on the 15th day of the experiment and tumor tissues were removed ambuloc. At the end of the experiment, all animals were sacrificed under ether anesthesia. Results: The MDA and GSH parameters in the cardiac blood taken from the subjects were examined and the diameters of the tumor tissues were measured and examined under a light microscope. Weight change in all groups was not statistically significant (p>0.05). It was found that the increases and decreases in the MDA and GSH values of the light and dark groups were not statistically significant (p>0.05). It was determined that there was no statistically significant difference between the tumor diameters of the control light groups and the tumor diameters of the other light groups (p> 0.05). While the difference between the control of dark groups and the tumor diameters of Group K2 was not statistically significant (p>0.05), the increase in tumor diameter of the subjects of Group K3 and Group K4 was found to be statistically significant (p<0.05). Conclusion: As a result; in this study, which investigated the effects of melatonin on experimental EST in dark and light environments, it was revealed that it did not show any positive effect on MDA and GSH levels.

The Eurasia Proceedings of Science Technology Engineering and Mathematics, Dec 31, 2021

The Covid-19 pandemic, including our country, has affected 34 million people around the world and... more The Covid-19 pandemic, including our country, has affected 34 million people around the world and caused high death rates and still is. One of the most important points in this process is disinfection, and both surface and air disinfectants have been used frequently. Disinfectants, which are extremely diversified in the market, have started to create danger while protecting us from the virus. It is known that the disinfectants used prevent the contagion of the virus, but we do not have clear information about whether they have destructive effects on the healthy cells of the people who use them. Therefore, in our study, it was aimed to determine the effects of hand disinfectants produced by various brands on our healthy cells. In our study, first of all, the most widely used disinfectants were obtained from the market, and then the cytotoxic (lethal) activity of these disinfectants on healthy cells was determined by in vitro MTT ((3-(4,5-Dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide) analysis. As a result of this study, it was observed that apart from the disinfectant containing hypochlorous acid, other disinfectants have cytotoxic activity against healthy human cells. Despite the increasing need during the pandemic process, we recommend that these disinfectant substances, which are put on the market in large numbers and in a wide variety, should be inspected and necessary measures should be taken so that they can be sold after passing through the quality control stages.

The Eurasia Proceedings of Science Technology Engineering and Mathematics, Aug 19, 2018

Pistacia vera fruit, one of the leading products of today's sweet and snack sector, which has bee... more Pistacia vera fruit, one of the leading products of today's sweet and snack sector, which has been seen in the history of the kingdom, are able to support daily nutritional needs and treatment of many diseases due to the phenolic and flavonoid content in the literature. Especially the studies on colon cancer and breast cancer are remarkable potential antitumoral activity of pistachio examined. The aim of this study is to examine in more detail the P. vera plant, in which we have previously tested the methanol-hexane extracts of the seed and the test and obtained positive results. As well as observing the cytotoxic activities of the plant extracts and active ingredients obtained from different parts on the lung cancer cells. After counting A549 and HUVEC cell lines which replicated in the medium, were added on 96 well plate. Then, different part of plants extract and 2 major active ingredients also added. Then MTT dye was applied and measured spectrophotometrically. P. vera extracts and active ingredients which studied cytotoxic activity research were found to be effective in all cell lines in general. Particularly P. vera's remaining after the fruit parts, methanol extract which obtained from waste containing leaf and stem parts and PVK-1 active agent showed selective activity on normal cells and cancer cells, therefore we consider that it has a high therapeutic index. We believe that this plant should be imparted to sciences and health sector with further studies.

Zenodo (CERN European Organization for Nuclear Research), Jun 30, 2021

The aim of this study was to present information about the traditional use and phytochemistry of ... more The aim of this study was to present information about the traditional use and phytochemistry of T. polium, to discuss contradictory views about chemotaxonomy and its toxic effect on liver and kidneys, and to make suggestions about controversial areas and gaps in the literature. Literature data showed that T. polium has toxic effect on kidney tissue. Moreover, in some of the studies on the liver and in all clinical reports, T. polium has also been proven to have toxic effect on the liver. The components responsible for toxicity are thought to be neo-clerodane diterpenoids. However, it has been reported that flavonoids and some polyphenols in the plant also show antioxidant and anti-inflammatory effects. It has been concluded that more attention should be paid to the use of this plant. More clinical studies are needed to better understand the effects of T. polium on the liver. The effects of the plant on blood serum parameters and histological changes on the liver tissue should be documented in more detail. It was also concluded that that regular consumption of T. polium should be avoided for long periods of time.

Cellular and Molecular Biology

Early diagnosis of breast cancer can increase the survivability of the patients and the patient’s... more Early diagnosis of breast cancer can increase the survivability of the patients and the patient’s quality of life. There is growing evidence demonstrating the active role of LncRNA-GAS5 and miR-103 in cancer biology. APOBEC enzymes are important players in immunity and may contribute to carcinogenesis. Mutation and expression alteration in the APOBEC gene family was found to have a strong correlation with breast cancer risk. This study aimed to evaluate the expression level of lncRNA-GAS5 and its target APOBEC3C in women with breast cancer through expression evaluation of miR-103. Moreover, the interaction between lncRNA-GAS5 and miR-103 was studied. In the present study, forty paired tumor and normal samples classified based on breast cancer subtypes and clinical features of patients were analyzed using gene expression studies. Immunohistochemical analysis of the gene products was performed to classify tumors. The RNA samples were extracted from breast tissue. Real-time PCR was con...

The Eurasia Proceedings of Science Technology Engineering and Mathematics, Dec 31, 2020

It is estimated that 1.3 billion waste is produced annually in the world and this amount is expec... more It is estimated that 1.3 billion waste is produced annually in the world and this amount is expected to increase to 38-67% by the end of 2025. Considering the waste production of pistachio, it is seen that around 132 165 thousand tons of waste products have been accumulated after the product processing phase due to excessive consumption and wide usage area. However, it has been reported that there are important secondary metabolites such as "masticadienonic acid", "masticadienolic acid", "tirucallol" and "pistasionic acid" in the extract content of unused waste stem parts. Masticadienonic acid has been shown in the literature to have antiproliferative and apoptotic activity. It has been determined that masticadienolic acid component has cytotoxic activity on five different cancer cell lines (Leukemia, Breast, Prostate, Colon and CNS). Examining the studies on the Tirukallol component, it was determined that it inhibits adhesion molecules in human endothelial cells. Pistacionic acid is a newly purified and characterized compound and only anticholinesterase and antidiabetic activities have been studied. The aim of our study is to determine whether these components obtained from stem parts have any cytotoxic effect on Mcf7 cells. Within the scope of the study, the stems were obtained from pistachio processing plants and kept at room temperature, in a cool environment. In order to obtain the compounds from sap extracts, column chromatography, ion layer chromatography (TLC) and crystallization methods which are moving phase systems were used. Mcf7 cells were exposed to different concentrations of the purified components and their cytotoxic activity was evaluated using the MTT test. Mcf7 cells were exposed to different concentrations of the purified components and their cytotoxic activity was evaluated using the MTT test. The results of our study are important preliminary data in the literature and we believe that they may contribute to further studies.

African Journal of Biotechnology, 2008

Lawsonia inermis commonly is used as cosmetic material. However many studies had shown that L. in... more Lawsonia inermis commonly is used as cosmetic material. However many studies had shown that L. inermis had antitumoural, antimicrobial and anti-tuberculostatic effects. The aim of this study was to investigate whether L. inermis can destroy cancer cells by induction of apoptosis due to decreasing of intracellular H+ ion level or increasing intracellular free radicals and H2O2 levels in cancer cells as a result of oxidative effect or not. We used 70 female Swiss albino mice and divided them into four groups. Group 1 was given only tap water. Group 2 was given only L. inermis. Group 3 was given Ehrlich ascites tumour (EAT) cells + tap water and Group 4 was given EAT + L. inermis. At the result of this study the thickness of subcutaneous lipid tissue, diameters of gluteal mass, the pH levels of gluteal mass, the GSH levels at the liver tissue samples and the MDA levels of the liver tissue samples of these groups were measured. This study showed that, L. inermis can be used as a supplem...

Turkiye’de ozellikle Karadeniz bolgesinde yetisen Corylus avellana L. ( C. avellana L.) turu find... more Turkiye’de ozellikle Karadeniz bolgesinde yetisen Corylus avellana L. ( C. avellana L.) turu findiktan yilda 10,000 tondan daha fazla findik yapragi ve 300 bin tondan fazla da yesil kabuk artigi aciga cikmaktadir. Bu nedenle bu calismamizda findik yesil yaprak ve kabugunun biyolojik aktivitelerini belirlemek istedik. Bu calismada, findik yesil kabugu ve yapragi metanol ile oda sicakliginda uygun optimizasyon kosullarinda 72 saat muamele edilerek ektraktlari elde edilmistir. Elde edilen ekstraklardan antibakteriyal aktivite testleri 8 farkli standart bakteri susu kullanilarak agar kuyu difuzyon yontemi kullanilarak analiz edilmistir. Ekstraklarda antiradikalik aktivite tayini icin DPPH (1,1-difenil-2-pikrilhidrazil) yontemi kullanilmistir. Bu calismada ekstraklarin H 2 O 2 ve UV-C’ye maruz birakilarak olusan DNA hasari uzerine etkileri pBR322 plazmid DNA kullanilarak arastirilmistir. Calismanin sonucunda findik yesil yaprak ve kabuklarinin antiradikalik aktivite gosterdikleri tespit ...

DergiPark (Istanbul University), Dec 31, 2020

A commercial strain of Candida albicans10231 was grown and isolated using Sabouraud's Dextrose Ag... more A commercial strain of Candida albicans10231 was grown and isolated using Sabouraud's Dextrose Agar Media. The samples were then subjected to lyophilization before protein extraction. TCA/acetone/methanol/phenol method has been used for protein extraction. For estimation of the percentage of extracted protein, BCA Protein Macro Assay Kit was applied. The samples were then transferred to the spectrophotometer for reading at wave length of 562 nm. The results show that the profile of the quantity of protein concentrations obtained upon supplementation of the following chemicals: PBS, ammonium bicarbonate, and 50mM of SDS buffer, were 0.133mg/mL, 0.080mg/mL, and 0.068mg/mL respectively.

International Journal of Pharmacology, Aug 1, 2016

The American Journal of Chinese Medicine, 2007

The aim of this study is to investigate the antitumor activity of Plantago major L. extract in Eh... more The aim of this study is to investigate the antitumor activity of Plantago major L. extract in Ehrlich ascites tumor (EAT) bearing Balb/C mice in vivo. Thirty male Balb/C mice were divided into 5 groups: 3 treatment groups and 2 control groups (6 per group). Treatment groups and the negative control group were injected with EAT (1 x 10(6) cells) intraperitoneally to develop ascites tumor. P. major L. extract (1%, 2% and 3% concentration extracts, 0.1 ml/day/mouse) were given p.o. for 10 alternate days. The control group was treated with 0.9% NaCl solution (0.1 ml/day/mouse). The changes of body weight in animals were recorded. On the 11th day, all of the mice were sacrified and their tissues were stained with haematoxylen and eosin for pathological studies. Body weights of in 3 treatment groups and the negative control group were elevated because of tumor burden. The maximal weight gain was recorded in the negative control group and the minimal weight gain was recorded in Group I. Pathological studies showed that P. major L. extract (especially 1% concentration) has inhibitive effect on EAT. P. major has an inhibitory effect on EAT in a dose dependent manner.

DergiPark (Istanbul University), Dec 31, 2020

Allergy is an important health problem affecting public health. Allergic diseases occur when the ... more Allergy is an important health problem affecting public health. Allergic diseases occur when the immune system reacts to non-harmful substances with the effect of genetic predisposition and environmental factors. According to the data of the World Allergy Organization (WAO), the prevalence of allergies in different countries varies between 10-40%. Pollen, mold, animal hair, house dust mite, medicines, and foods are the most common allergen agents. Common mushrooms in nature have the potential to produce allergenic proteins. Penicillium, Aspergillus, Rhizopus, and Mucor species, which are allergic fungi, are widely found in nature. In our country, 614 patients with respiratory allergy have been reported to develop allergic reactions against Aspergillus fumigatus, Trichophyton rubrum, Mucor, Penicillium notatum, Aspergillus niger, and Alternaria tenius. In recent years, the cases of allergies caused by molds have increased significantly and studies to determine the causing allergens have accelerated. Mucor mucedo (brown bread mold) was used in our study. Mucor mucedo produced in our laboratory was collected and allergen fungus protein was extracted by 2 different extraction methods. By preparing protein samples from prepared mushroom extracts, the total concentration of potential allergen proteins was determined by the BCA method. According to the data obtained, it was determined that the protein concentration of the mushroom samples dried by that were subjected to dialysis was higher than ethanol. As a result, Mucor mucedo was found to have a high protein concentration and revealed basic data for further analysis.

International Journal of Applied Research in Natural Products, Dec 14, 2012

This study was designed to address whether simultaneous primary chemo-hormonal therapy provides a... more This study was designed to address whether simultaneous primary chemo-hormonal therapy provides additional activity compared with chemotherapy alone in breast cancer patients with operable or locally advanced disease. Between January 1997 and January 2002, 211 consecutive patients with T2-4, N0-1, M0 breast cancer were randomized to receive either epirubicin alone (EPI) or epirubicin plus tamoxifen (EPI-TAM). Ki67 expression was evaluated immunohistochemically in tumor specimens obtained before chemotherapy by incision biopsy and at definitive surgery. Tumor shrinkage of >50% was obtained in 76% of patients randomized in the EPI arm and 81.9% of patients randomized in the EPI-TAM arm (not significant). The corresponding rates of clinical and pathological complete response were 20.2 and 21.9% (not significant), and 4.8 and 6.7% (not significant), respectively. Pathologically complete response was more frequently observed in estrogen receptor (ER)-negative (ER-) tumors (P=0.04) and correlated with elevated baseline Ki67 expression (P<0.01). Both EPI and EPI-TAM treatments resulted in a significant reduction in Ki67 expression, either in overall patients (P=0.000) or in patients with ER+ breast cancer (P=0.000). The reduction in Ki67 immunostaining in the EPI-TAM arm was greater than in the EPI arm, leading to a lower Ki67 expression at post-operative residual histology (P=0.0041). The addition of tamoxifen to epirubicin chemotherapy did not improve the response rate but led to a significantly higher reduction in the Ki67 expression. Baseline elevated Ki67 expression and the ERstatus were both associated with a greater chance of obtaining a pathological complete response at residual histology.

Plant Pathology Journal, Dec 15, 2006

Dünya'da alternatif tedavilerde, bitkisel ekstreler birçok hastalıkta tedavi amaçlı kullanılmakta... more Dünya'da alternatif tedavilerde, bitkisel ekstreler birçok hastalıkta tedavi amaçlı kullanılmaktadır. Ülkemizin farklı iklim ve ekolojik koşullara sahip olması, bulunan floranın sayısal açıdan yüksek oranda bitki türü ve çeşitliliği barındırmasından dolayı ülkemizde medikal amaçlı tüketimi yapılan çok sayıda bitki türü vardır. Bu bitkilerin çoğunun antimikrobiyal etkilerinin varlığı hem yurtdışında hem de ülkemizde yapılan araştırmalar sonucunda bildirilmiştir. Doğada kendiliğinden yetişen bazı bitki ekstrelerinin ve uçucu yağlarının bakterilere olduğu kadar, mantarlara karşı da antifungal aktivite gösterdiği yapılan çalışmalarda tespit edilmiştir. Bu çalışmada çıkarılan özütlerin Stenotrophomonas maltophilia bakterisine karşı antimiktorbiyal etkileri ve DNA koruyucu potansiyeli çalışılmıştır. Antimikrobiyal aktivite disk difüzyon yöntemi ile çalışılmıştır. DNA koruyucu aktivite pBR322 plazmid DNA'sı ile H2O2 ve UV-C kullanılarak çalışılmıştır. Su ve Methanol özütlerinin Stenotrophomonas maltophilia üzerine etkili olduğu saptanmıştır. Diğer yandan DNA koruyucu aktivite bakımından oldukça yüksek koruyucu aktivite gösterdiği çalışma kapsamında belirlenmiştir.

The Eurasia Proceedings of Science Technology Engineering and Mathematics, Aug 19, 2018

Allergic diseases are a major public health problem in the modern societies. Pollens dispersed th... more Allergic diseases are a major public health problem in the modern societies. Pollens dispersed through wind are one of the major aeroallergens. When they are released to atmosphere in sufficient amount, they can cause the development of diseases such as asthma, rhinitis, rhinoconjunctivitis etc. in allergically hypersensitive individuals. The spreading of pollens are influenced by their amount in the air, their structures, geographic areas and the climate. Therefore, the types of pollens that hypersensitive individuals who live in different regions are exposed to may differ and different allergic reactions may occur in affected individuals. The high amount of allergens in pollens enhance the sensitivity to pollens. Thus the research in region-specific plant species allergenic effects is very important. Pollens of linden (Tilia cordata), Anatolian oak (Quercus ithaburensis) and birch (Betula alba) are important allergen sources in Gaziantep province. Pollen allergens are water soluble, stable proteins or glycoproteins of molecular weight between 5-80 kD. A single pollen type usually contains several different allergens. Pollens from linden (T. cordata), Anatolian oak (Q. ithaburensis) and birch (B. alba) were collected during pollination period and their extracts were prepared. For identification of pollens morphology slides were prepared according to Wodehouse's method and images were taken under light microscope. Total concentrations of potential allergen proteins were determined from prepared pollen extracts using the BCA method. In this study, we aimed to prepare extracts of pollens from linden, Anatolian oak and birch widely grown in wooded areas of Gaziantep University for the study of allergens and their use for diagnosing allergic diseases.

The Eurasia Proceedings of Science Technology Engineering and Mathematics, Aug 19, 2018

Allergens can be defined as antigens that stimulate specific IgE (Immunoglobulin E) antibody form... more Allergens can be defined as antigens that stimulate specific IgE (Immunoglobulin E) antibody formation and react with it. In this study, the morphologies of Pistachia vera allergenic pollens belonging to Anacardiaceae family in South Eastern Anatolia were examined by light microscopy and pollens extracts were prepared from these species. The specimens used in morphological studies were obtained from Gaziantep University campus area. Slides of pollens were prepared according to Wodehouse (1935) method and the morphological characteristics of pollens were determined using microphotographs taken by light microscopy. Pollens crude extracts were obtaıned from the flora grown in this region. Extraction method established by Aytug et al. (1991) was used to prepare crude extracts from the collected pollens, 'Coca' solution was added extensively and sterile filtration technique was used for sterilization. BCA assay and absorbance measurements were used to determine the protein concentration. Standard curve was generated using concentrations and absorbance values of standard protein samples prepared at different concentrations (0, 1-1mg/ml). The protein concentration of the pollens extracts was calculated using the correct equation obtained from this curve. These crude extracts contain many major and minor allergens that can be used to diagnose allergic diseases. Due to the very high concentration of allergenic proteins in P. vera, it can be included in allergen kits. Moreover, allergy tests should be conducted to those living in this region. The results obtained from this study can both contribute to systematic studies and to the treatment of allergic diseases caused by these types of pollens.

African Journal of Biotechnology, Jun 27, 2011

Stethoscopes are one of the most commonly used medical devices and have been reported to be poten... more Stethoscopes are one of the most commonly used medical devices and have been reported to be potential sources of hospital acquired infections. In this study, we aimed to find out the bacterial contamination of stethoscopes used by health-care staff. Swab samples were taken from the surface of the diaphragm of the stethoscopes used by health personnel in four hospitals including three secondline and one third-line health care institution in Turkey. The samples were inoculated onto bacteriological and mycological media. For identification of the microorganisms, conventional methods and Vitek2 (Biomérieux) were performed. Swab samples were taken from 121 stethoscopes. We found bacterial and fungal contamination on 92 (76%) of the stethoscopes. 15 out 90 (16.3%) had potential pathogens including methicillin susceptible Staphylococcus aureus (5), methicillin resistance Staphylococcus aureus (4), Escherichia coli (3), Acinetobacter baumannii, Acinetobacter haemolyticus and Enterococcus spp. Of the 121 health-care persons, only 61 regularly cleaned their stethoscopes by various disinfectants. The statistical difference between the two groups in terms of pathogen and microorganism isolation was not determined (p>0.05). Although stethoscopes are uncritical medical devices, they could contain pathogen microorganisms and they might be a potential source of hospital acquired infections.

Phosphorus Sulfur and Silicon and The Related Elements, Oct 11, 2013

Abstract The synthesis and characterization of 18 novel thiosemicarbazones have been investigated... more Abstract The synthesis and characterization of 18 novel thiosemicarbazones have been investigated as part of a research program on development of compounds with antibacterial and antioxidant activities. Among the tested compounds, 2-(4-hydroxybenzylidene)-N-[4-(trifluoromethoxy)phenyl]hydrazine carbothioamide (3g) and 2-(thiophen-2-ylmethylidene)-N-[4-(morpholin-4-yl)phenyl]hydrazine carbothioamide (4b) showed excellent inhibition potency at low concentration (0.5 μg/mL) against Gram-positive pathogens (Enterococcus faecalis and Staphylococcus aureus). All tested compounds were also found to possess antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation. [Supplementary materials are available for this article. Go to the publisher's online edition of Phosphorus, Sulfur, and Silicon and the Related Elements for the following free supplemental files: Additional text, tables, and figures.] GRAPHICAL ABSTRACT

Journal of chemistry and nutritional biochemistry, Dec 30, 2022

Background: In this study, it was aimed to test the anti-tumoral effects of melatonin in vivo. In... more Background: In this study, it was aimed to test the anti-tumoral effects of melatonin in vivo. In the literature review, no study was found that examined the anti-tumoral effect of melatonin at night on an experimental tumor model in vivo. Therefore, in the study, the anti-tumoral effect of melatonin was tested in solid tumor tissue formed in Swiss albino male mice. Methods: Swiss albino male mice formed Swiss albino male mice in vivo and 80 Swiss albino male mice weighing 25-30 g and aged 10-12 weeks were used. The subjects were divided into 10 groups in total (n=8). These groups are divided into two main groups as light and dark. All animals except the control group were injected intramuscularly (i.m.) with EAT to create EST in the right leg scapula of the subjects. Different doses of melatonin were injected intraperitoneally (i.p.) daily to the experimental groups. EST was created by inoculating EAT 2 days after administration of melatonin. Melatonin application was made between 18:00 and 20:00 in the evening. In addition, light groups were exposed to 580 µW fluorescent light between 02:00 and 04:00 at night. Daily food and water consumption and weight gains of all animals were followed and recorded, and the application was continued for 14 days. Cardiac blood was collected from all animals with heparinized syringes on the 15th day of the experiment and tumor tissues were removed ambuloc. At the end of the experiment, all animals were sacrificed under ether anesthesia. Results: The MDA and GSH parameters in the cardiac blood taken from the subjects were examined and the diameters of the tumor tissues were measured and examined under a light microscope. Weight change in all groups was not statistically significant (p>0.05). It was found that the increases and decreases in the MDA and GSH values of the light and dark groups were not statistically significant (p>0.05). It was determined that there was no statistically significant difference between the tumor diameters of the control light groups and the tumor diameters of the other light groups (p> 0.05). While the difference between the control of dark groups and the tumor diameters of Group K2 was not statistically significant (p>0.05), the increase in tumor diameter of the subjects of Group K3 and Group K4 was found to be statistically significant (p<0.05). Conclusion: As a result; in this study, which investigated the effects of melatonin on experimental EST in dark and light environments, it was revealed that it did not show any positive effect on MDA and GSH levels.

The Eurasia Proceedings of Science Technology Engineering and Mathematics, Dec 31, 2021

The Covid-19 pandemic, including our country, has affected 34 million people around the world and... more The Covid-19 pandemic, including our country, has affected 34 million people around the world and caused high death rates and still is. One of the most important points in this process is disinfection, and both surface and air disinfectants have been used frequently. Disinfectants, which are extremely diversified in the market, have started to create danger while protecting us from the virus. It is known that the disinfectants used prevent the contagion of the virus, but we do not have clear information about whether they have destructive effects on the healthy cells of the people who use them. Therefore, in our study, it was aimed to determine the effects of hand disinfectants produced by various brands on our healthy cells. In our study, first of all, the most widely used disinfectants were obtained from the market, and then the cytotoxic (lethal) activity of these disinfectants on healthy cells was determined by in vitro MTT ((3-(4,5-Dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide) analysis. As a result of this study, it was observed that apart from the disinfectant containing hypochlorous acid, other disinfectants have cytotoxic activity against healthy human cells. Despite the increasing need during the pandemic process, we recommend that these disinfectant substances, which are put on the market in large numbers and in a wide variety, should be inspected and necessary measures should be taken so that they can be sold after passing through the quality control stages.

The Eurasia Proceedings of Science Technology Engineering and Mathematics, Aug 19, 2018

Pistacia vera fruit, one of the leading products of today's sweet and snack sector, which has bee... more Pistacia vera fruit, one of the leading products of today's sweet and snack sector, which has been seen in the history of the kingdom, are able to support daily nutritional needs and treatment of many diseases due to the phenolic and flavonoid content in the literature. Especially the studies on colon cancer and breast cancer are remarkable potential antitumoral activity of pistachio examined. The aim of this study is to examine in more detail the P. vera plant, in which we have previously tested the methanol-hexane extracts of the seed and the test and obtained positive results. As well as observing the cytotoxic activities of the plant extracts and active ingredients obtained from different parts on the lung cancer cells. After counting A549 and HUVEC cell lines which replicated in the medium, were added on 96 well plate. Then, different part of plants extract and 2 major active ingredients also added. Then MTT dye was applied and measured spectrophotometrically. P. vera extracts and active ingredients which studied cytotoxic activity research were found to be effective in all cell lines in general. Particularly P. vera's remaining after the fruit parts, methanol extract which obtained from waste containing leaf and stem parts and PVK-1 active agent showed selective activity on normal cells and cancer cells, therefore we consider that it has a high therapeutic index. We believe that this plant should be imparted to sciences and health sector with further studies.

Zenodo (CERN European Organization for Nuclear Research), Jun 30, 2021

The aim of this study was to present information about the traditional use and phytochemistry of ... more The aim of this study was to present information about the traditional use and phytochemistry of T. polium, to discuss contradictory views about chemotaxonomy and its toxic effect on liver and kidneys, and to make suggestions about controversial areas and gaps in the literature. Literature data showed that T. polium has toxic effect on kidney tissue. Moreover, in some of the studies on the liver and in all clinical reports, T. polium has also been proven to have toxic effect on the liver. The components responsible for toxicity are thought to be neo-clerodane diterpenoids. However, it has been reported that flavonoids and some polyphenols in the plant also show antioxidant and anti-inflammatory effects. It has been concluded that more attention should be paid to the use of this plant. More clinical studies are needed to better understand the effects of T. polium on the liver. The effects of the plant on blood serum parameters and histological changes on the liver tissue should be documented in more detail. It was also concluded that that regular consumption of T. polium should be avoided for long periods of time.

Cellular and Molecular Biology

Early diagnosis of breast cancer can increase the survivability of the patients and the patient’s... more Early diagnosis of breast cancer can increase the survivability of the patients and the patient’s quality of life. There is growing evidence demonstrating the active role of LncRNA-GAS5 and miR-103 in cancer biology. APOBEC enzymes are important players in immunity and may contribute to carcinogenesis. Mutation and expression alteration in the APOBEC gene family was found to have a strong correlation with breast cancer risk. This study aimed to evaluate the expression level of lncRNA-GAS5 and its target APOBEC3C in women with breast cancer through expression evaluation of miR-103. Moreover, the interaction between lncRNA-GAS5 and miR-103 was studied. In the present study, forty paired tumor and normal samples classified based on breast cancer subtypes and clinical features of patients were analyzed using gene expression studies. Immunohistochemical analysis of the gene products was performed to classify tumors. The RNA samples were extracted from breast tissue. Real-time PCR was con...

The Eurasia Proceedings of Science Technology Engineering and Mathematics, Dec 31, 2020

It is estimated that 1.3 billion waste is produced annually in the world and this amount is expec... more It is estimated that 1.3 billion waste is produced annually in the world and this amount is expected to increase to 38-67% by the end of 2025. Considering the waste production of pistachio, it is seen that around 132 165 thousand tons of waste products have been accumulated after the product processing phase due to excessive consumption and wide usage area. However, it has been reported that there are important secondary metabolites such as "masticadienonic acid", "masticadienolic acid", "tirucallol" and "pistasionic acid" in the extract content of unused waste stem parts. Masticadienonic acid has been shown in the literature to have antiproliferative and apoptotic activity. It has been determined that masticadienolic acid component has cytotoxic activity on five different cancer cell lines (Leukemia, Breast, Prostate, Colon and CNS). Examining the studies on the Tirukallol component, it was determined that it inhibits adhesion molecules in human endothelial cells. Pistacionic acid is a newly purified and characterized compound and only anticholinesterase and antidiabetic activities have been studied. The aim of our study is to determine whether these components obtained from stem parts have any cytotoxic effect on Mcf7 cells. Within the scope of the study, the stems were obtained from pistachio processing plants and kept at room temperature, in a cool environment. In order to obtain the compounds from sap extracts, column chromatography, ion layer chromatography (TLC) and crystallization methods which are moving phase systems were used. Mcf7 cells were exposed to different concentrations of the purified components and their cytotoxic activity was evaluated using the MTT test. Mcf7 cells were exposed to different concentrations of the purified components and their cytotoxic activity was evaluated using the MTT test. The results of our study are important preliminary data in the literature and we believe that they may contribute to further studies.

African Journal of Biotechnology, 2008

Lawsonia inermis commonly is used as cosmetic material. However many studies had shown that L. in... more Lawsonia inermis commonly is used as cosmetic material. However many studies had shown that L. inermis had antitumoural, antimicrobial and anti-tuberculostatic effects. The aim of this study was to investigate whether L. inermis can destroy cancer cells by induction of apoptosis due to decreasing of intracellular H+ ion level or increasing intracellular free radicals and H2O2 levels in cancer cells as a result of oxidative effect or not. We used 70 female Swiss albino mice and divided them into four groups. Group 1 was given only tap water. Group 2 was given only L. inermis. Group 3 was given Ehrlich ascites tumour (EAT) cells + tap water and Group 4 was given EAT + L. inermis. At the result of this study the thickness of subcutaneous lipid tissue, diameters of gluteal mass, the pH levels of gluteal mass, the GSH levels at the liver tissue samples and the MDA levels of the liver tissue samples of these groups were measured. This study showed that, L. inermis can be used as a supplem...

Turkiye’de ozellikle Karadeniz bolgesinde yetisen Corylus avellana L. ( C. avellana L.) turu find... more Turkiye’de ozellikle Karadeniz bolgesinde yetisen Corylus avellana L. ( C. avellana L.) turu findiktan yilda 10,000 tondan daha fazla findik yapragi ve 300 bin tondan fazla da yesil kabuk artigi aciga cikmaktadir. Bu nedenle bu calismamizda findik yesil yaprak ve kabugunun biyolojik aktivitelerini belirlemek istedik. Bu calismada, findik yesil kabugu ve yapragi metanol ile oda sicakliginda uygun optimizasyon kosullarinda 72 saat muamele edilerek ektraktlari elde edilmistir. Elde edilen ekstraklardan antibakteriyal aktivite testleri 8 farkli standart bakteri susu kullanilarak agar kuyu difuzyon yontemi kullanilarak analiz edilmistir. Ekstraklarda antiradikalik aktivite tayini icin DPPH (1,1-difenil-2-pikrilhidrazil) yontemi kullanilmistir. Bu calismada ekstraklarin H 2 O 2 ve UV-C’ye maruz birakilarak olusan DNA hasari uzerine etkileri pBR322 plazmid DNA kullanilarak arastirilmistir. Calismanin sonucunda findik yesil yaprak ve kabuklarinin antiradikalik aktivite gosterdikleri tespit ...