Amir Sohail | Govt.College University Faisalabad Pakistan (original) (raw)

Papers by Amir Sohail

Agronomy, 2022

Rice (Oryza sativa L.) and Arabidopsis thaliana (L.) life cycles involve several major phase chan... more Rice (Oryza sativa L.) and Arabidopsis thaliana (L.) life cycles involve several major phase changes, throughout which MADS-box genes have a variety of functions. MADS-box genes are well recognized for their functions in floral induction and development, and some have multiple functions in apparently unrelated developmental stages. For example, in Arabidopsis, AGL15 and AGL6 play roles in both vegetative development and floral transition. Similarly, in rice, OsMADS1 is involved in flowering time and seed development, and OsMADS26 is expressed not only in the roots, but also in the leaves, shoots, panicles, and seeds. The roles of other MADS-box genes responsible for the regulation of specific traits in both rice and Arabidopsis are also discussed. Several are key components of gene regulatory networks involved in root development under diverse environmental factors such as drought, heat, and salt stress, and are also involved in the shift from vegetative to flowering growth in respo...

This research was carried out to check genetic variability, heritability and genetic advance in 1... more This research was carried out to check genetic variability, heritability and genetic advance in 11 F4 bread wheat (Triticum aestivum L.) genotypes (10 F4 lines and one check) in a randomized block design with three replications at the University of Agriculture Peshawar, Pakistan during 2015-16. Data was/were taken on parameters such as days to heading (days), plant height (cm), flag leaf area (cm 2), spike length (cm), grain weight spike-1 (g), 1000-grain weight (g), grain yield plant-1 (g), biological yield plant-1 (g) and harvest index (%). The statistically significant difference(s) was/were detected for the investigated traits. The high magnitude of heritability (˃0.62) was noted for all parameters except spike length (0.57) which was moderate. Low expected genetic advance was recorded for days to heading (3.90%) and spike length (8.13%), moderate expected genetic advance was observed for plant height (9.95%), grain weight spike-1 (11.54%) and 1000 grain weight (13.41%), while h...

2020 3rd International Conference on Computing, Mathematics and Engineering Technologies (iCoMET), 2020

Electricity shortages are severe for developing countries like Pakistan as a result of massive po... more Electricity shortages are severe for developing countries like Pakistan as a result of massive population growth. To solve this problem Renewable energy resources (RER’s) and energy storage systems (ESS) can play a considerable role. In this paper, we have analyzed the effects on the operational cost of residential electricity consumers by installing the rooftop photovoltaic (PV) and ESS on their smart homes. Additionally, the home to grid (H2G) energy exchange framework is also included to explore its benefits on energy management. Home Energy Management (HEM) problem is formulated with the integration of PV and ESS by considering user preferences and other system boundaries. A Grey wolf optimization (GWO) algorithm is used to find out optimal scheduling of appliances. The findings of the simulations indicate that the proposed solution can improve the performance of the home electricity scheduling, reduced the effect of uncertainty on the system and decrease the overall energy consumption cost.

PAFMJ, 2021

Objective: To compare the pain relief efficacy of platelet rich plasma injection with corticoster... more Objective: To compare the pain relief efficacy of platelet rich plasma injection with corticosteroid injection in knee osteoarthritis using numeric rating scale. Study Design: Quasi experimental study. Place and Duration of Study: Department of Anesthesia and Pain Management, Combined Military Hospital Peshawar, from Jan 2018 to Dec 2019. Methodology: Total of 310 patients who underwent knee injection for osteoarthritis were included in this study. Patients were divided into two groups; group A and group B comprising of 155 patients each. Patients in group ‘A’ received intra articular corticosteroid injection while patients in group ‘B’ received intra articular platelet rich plasma injection for knee osteoarthritis. Pain assessment via numerical rating score was done at the start of the treatment and at 6 months. Results: In group A female to male ratio was 2.69:1 while in group B the female to male ratio was 2.78:1. Mean age of ‘group A’ was 58.52 ± 11.87 years and that of ‘group B...

Pakistan Journal of Phytopathology, 2021

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Objective: To compare patients undergoing cesarean section under spinal and general anesthesia in... more Objective: To compare patients undergoing cesarean section under spinal and general anesthesia in terms of neonatal APGAR scores and patient satisfaction. Study Design: Comparative cross-sectional study. Place and Duration of Study: Departments of Anesthesia and Gynecology and Obstetrics, Combined Military Hospital Peshawar, for six months from Mar to Aug 2019. Methodology: In this study, 120 females with a singleton pregnancy of 36-40 weeks, and requiring a non-emergency elective cesarean section were enrolled through consecutive sampling in the study. The patients were randomly allocated into two equal groups of 60 women each through random table numbers. Group A received spinal anesthesia and group B general anesthesia. APGAR score was assessed at 5 minutes after delivery, and score of ≥7 was taken as satisfactory. Patient's satisfaction level regarding anesthesia was recorded on a visual analogue scale of 0 (extremely unsatisfied) to 10 (completely satisfied), and score of ≥...

Purpose – The research study aims to assess the women’s Business education and leadership trainin... more Purpose – The research study aims to assess the women’s Business education and leadership training program at a higher institutional level in order to examine how the program impacts and contributes to women’s empowerment. Further, this research sought to explore women’s leadership training aspect that has not explicitly elucidated. Besides, this study looks upon women’s self-perception by empowering them with skills and leadership capabilities to change hard-core gender stereotypes in society. Research methodology – The sample size comprised of 82 enrolled female students in the training program. The qualitative statistics (i.e., three focus groups) evaluated with the Kirkpatrick model. The Wilcoxon signed-rank test was applied to compare pre-and post-training quantitative data (i.e., questionnaire). Effect size is measured to check the effectiveness of the training program. Findings – The post-training evaluation revealed higher scores in knowledge, tools, and leadership skills. T...

From assessing the fertility status of soil, 20 soil samples were collected from different locati... more From assessing the fertility status of soil, 20 soil samples were collected from different location of district Dargai area and analyzed in the laboratory of Soil and Environmental Sciences Department, the University of Agriculture-Peshawar. The soil samples were found medium in texture. Almost all soils have alkaline PH, and from moderately to strongly calcareous. The organic matter content was in the range of marginal and deficient. AB-DTPA extractable P was deficient in 11 soil samples, while adequate in 3 soil samples and marginal in 4 soil samples. The K content is deficient in 13 soil samples while 7 soil samples were marginal. The data collected from physical and chemical analysis of soil samples were statistically analyzed by using standard statistical procedure as described by Steel and Terrie [1]. The result obtained showed that there were significant differences among the physico-chemical properties of soil of Dargai. Dargai soils are non-saline in all soil depth and alka...

Recurrent selection is a vital selection method for improving the traits of interest in maize cro... more Recurrent selection is a vital selection method for improving the traits of interest in maize crop. This research was carried out to estimate genetic variability, heritability, index of variation and genotypic and phenotypic correlations among half sib recurrent families for various traits. Sixty four half sib recurrent families were evaluated in 8×8 lattice square design with two replications at Cereal Crops Research Institute CCRI, Pirsabak during 2017. Results showed highly significant differences among the half sib families for all the studied traits. High heritability (h2 ˃ 0.60) were recorded for all traits except plant height and ear height which exhibited moderate heritability. High index of variation (I.V ˃ 1) was observed for all traits. After completion of one cycle of recurrent selection in half sib families, the percent gain cycle-1 was recorded negative for physio-morphic traits, while for grain yield the percent gain cycle-1 was positive. All physiological traits show...

Recurrent selection is a cyclic selection procedure for improving the mean performance of plant p... more Recurrent selection is a cyclic selection procedure for improving the mean performance of plant populations. The goal of current research was to determine the response of recurrent selection in CIMMYT maize population CZP-132011 using half sib families and to estimate selection differential, heritability, expected response and percent gain cycle-1 for yield attributes. Sixty four half sib recurrent families were evaluated in 8×8 lattice square design with two replication at Cereal Crops Research Institute (CCRI), Pirsabak during 2017. Results showed highly significant differences among the half sib families for all the traits. Selection differential values were positive for yield and yield attributing traits. High heritability values (h2 ˃ 0.60) were recorded for all traits except 1000 grain weight (0.54), which exhibited moderate heritability. Based on broad sense heritability and selection differential, the expected response were positive for ears plant1 (0.15), ear length (1.48 c...

This study was planned for the production of lipases by solid-state fermentation (SSF) using agro... more This study was planned for the production of lipases by solid-state fermentation (SSF) using agro-industrial wastes as substrate. A total of 14 bacterial strains were isolated, screened and compared for lipase production. Among the evaluated strains, Bacillus subtilis strain NL-39 was selected on the basis of best lipase production. The maximum enzyme activity (34.93 U/ml) was shown by B. subtilis using SSF with soya bean meal. The lipase production by other low cost agro-industrial waste, including sunflower meal, wheat bran, oat bran, rice bran and sugar cane bagasse was 23.83, 12.17, 10.40 10.00 and 16.23 U/ml, respectively. The lipase production by B. subtilis using peels of different fruits, including banana, orange, water melon and melon as carbon source, was 27.17, 21.37, 10.57 and 8.43 U/ml, respectively. The corn cob produced 12.27 U/ml while waste oils of various industries produced 16.17 U/ml (Shan oil), 13.67 U/ml (automobile), 13.37 U/ml (unbranded waste cooking oil) an...

Frontiers in Genetics, 2021

In angiosperms, anther development comprises of various complex and interrelated biological proce... more In angiosperms, anther development comprises of various complex and interrelated biological processes, critically needed for pollen viability. The transitory callose layer serves to separate the meiocytes. It helps in primexine formation, while the timely degradation of tapetal cells is essential for the timely callose wall dissolution and pollen wall formation by providing nutrients for pollen growth. In rice, many genes have been reported and functionally characterized that are involved in callose regulation and pollen wall patterning, including timely programmed cell death (PCD) of the tapetum, but the mechanism of pollen development largely remains ambiguous. We identified and functionally characterized a rice mutant dcet1, having a complete male-sterile phenotype caused by defects in anther callose wall, exine patterning, and tapetal PCD. DCET1 belongs to the RNA recognition motif (RRM)-containing family also called as the ribonucleoprotein (RNP) domain or RNA-binding domain (R...

The paper examines the Impact of foreign direct investment (FDI) on economic growth in Pakistan. ... more The paper examines the Impact of foreign direct investment (FDI) on economic growth in Pakistan. The study has used data from 2000 to 2010 by using Two- Stage least squares method of simultaneous equations the results have been estimated. The results show that there exists a negative relationship between economic growth, proxies by gross domestic product (GDP) and foreign direct investment in Pakistan. Domestic investment, exports size and political stability were found to be very appropriate in locational choice of foreign direct investment in Pakistan. The enhancement of foreign direct investment in Pakistan government should ensure political stability and encourage more domestic investment and also make sure about trade globalization and foreign direct investments policies in today’s era for attracting more foreign direct investment in Pakistan. Keywords : FDI, GDP, Exports Growth and Domestic Investment.

Analytica Chimica Acta, 2020

Affinity chromatography is a powerful technology for phosphopeptide enrichment from body fluids. ... more Affinity chromatography is a powerful technology for phosphopeptide enrichment from body fluids. Saliva is a non-invasive body fluid for disease diagnosis, while few studies applied affinity enrichment for saliva phosphoproteome. In this study, we tested two kinds of affinity chromatography materials, Ti4+-IMAC (immobilized metal affinity chromatography) and CaTiO3, for the enrichment of phosphopeptides. Through comparison, Ti4+-IMAC method was demonstrated as the superior one, which was utilized for the comprehensive analysis of salivary phosphoproteome. More than 360 phosphoproteins were specifically extracted and identified from human saliva. Ti4+-IMAC method was further applied to compare the phosphoprotein profiling in the saliva of lung cancer group and normal control group through label-free quantification. Accordingly, 477 and 699 phosphopeptides were enriched, respectively, which corresponded to 339 and 466 proteins. In total, 796 unique phosphopeptides were revealed for 517 saliva phosphoproteins. In particular, 709 phosphorylation sites were identified, among which 26 were up-regulated (>1.5) and 149 were down-regulated (<0.66) in lung cancer. Their corresponding proteins were mainly associated with cancer promotion, system disorder, and organismal injury. Our data collectively demonstrated that salivary phosphopeptides can be comprehensively characterized through Ti4+-IMAC method. These discovered phosphoprotein candidates might be used for lung cancer detection through salivary diagnostics.

Molecules, 2021

Introduction: The most common forms of vitamin D in human and mouse serum are vitamin D3 and vita... more Introduction: The most common forms of vitamin D in human and mouse serum are vitamin D3 and vitamin D2 and their metabolites. The aim of this study is to determine whether diet and sunlight directly affect the circulating concentrations of vitamin D metabolites in a mouse model. We investigated the serum concentrations of eight vitamin D metabolites—vitamin D (vitamin D3 + vitamin D2), 25OHD (25OHD3 + 25OHD2), 1α25(OH)2D (1α25(OH)2D2, and 1α25(OH)2D3)—including their epimer, 3-epi-25OHD (3-epi-25OHD3 and 3-epi-25OHD2), and a bile acid precursor 7alpha-hydroxy-4-cholesten-3-one (7αC4), which is known to cause interference in liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Method: The LC-MS/MS method was validated according to FDA-US guidelines. The validated method was used for the analysis of mouse serum samples. Forty blood samples from mice were collected and divided into three groups. The first group, the DDD mice, were fed a vitamin D-deficient diet (25 IU V...

2021 International Conference on Content-Based Multimedia Indexing (CBMI), 2021

Biochimie, 2020

Hepatocellular carcinoma (HCC) is a common neoplastic transformation of the hepatocytes, which ha... more Hepatocellular carcinoma (HCC) is a common neoplastic transformation of the hepatocytes, which has high morbidity and mortality worldwide, particularly in Eastern Asia. HCC is also developed as a consequence of chronic liver cirrhosis, and both diseases are difficult to diagnosis and differentiate. Accurate noninvasive biomarkers for HCC and cirrhosis are urgently needed. In the search for novel candidates, small extracellular vesicles (sEVs) were isolated from the serum of liver cancer patients, liver cirrhosis patients, healthy control subjects, as well as the culture media of hepatocellular carcinoma cells (HepG2) and normal hepatocyte cells (Lo2). Isolated sEVs were confirmed by size distribution analysis, morphological analysis, and surface biomarker tests. Mass spectrometry based label-free quantification revealed 61 and 63 differentially expressed proteins in the serum sEVs of liver cirrhosis patients and liver cancer patients (p < 0.05), respectively. The proteomics data of cell-derived sEVs were combined for the selection of valuable candidates. Promising proteins were further verified by immunoassay, including thrombospondin-1 (THBS1), fibulin-1(FBLN1), and fibrinogen gamma chain (FGG), which could differentiate healthy control from liver cancer or liver cirrhosis. Our findings verified the hypothesis that cancer-related proteomics signatures are present in the sEVs of patient's serum and might be monitored for the evaluation of liver cancer and liver cirrhosis.