Jie Zheng | Guangdong University of Foreign Studies (original) (raw)
Papers by Jie Zheng
Zhonghua Bing Li Xue Za Zhi Chinese Journal of Pathology, Feb 1, 2006
To study the clinicopathological features and differential diagnoses of mixed epithelial and stro... more To study the clinicopathological features and differential diagnoses of mixed epithelial and stromal tumor of the kidney. Clinical and pathological characteristics of 4 cases of mixed epithelial and stromal tumor of the kidney were studied. Three patients were female and one was male. All patients presented with flank pain and hematuria. Radiologic studies revealed cystic and solid masses involving the kidney. Grossly the tumors had a solid and cyst appearance. Microscopically, the tumors were composed of a mixture of stromal and epithelial elements. The epithelial elements were variable in cell types including cuboidal, hobnail and columnar cells. One case showed Müllerian and intestinal epithelial differentiations. Stromal elements essentially consisted of spindle cells, with thick-walled blood vessels and bands of smooth muscle cells as distinctive features of the tumor. Immunohistochemical staining revealed that the epithelial components were positive for AE1/AE3, whereas the stromal components were positive for ER, PR, and SMA. All patients underwent nephrectomy and were well without evidence of recurrence. Mixed epithelial and stromal tumor of the kidney is a benign neoplasm with distinct histopathological features. It should be distinguished from many other renal neoplasms. Surgical intervention is a preferred therapy.
2008 World Automation Congress, 2008
ABSTRACT
Progress in Biomedical Optics and Imaging, Feb 10, 2011
Zhongguo Yi Xue Ke Xue Yuan Xue Bao Acta Academiae Medicinae Sinicae, Apr 1, 2007
OBJECTIVE: To explore whether the antibody subtypes against the extracellular 1-2 (EC1-2) epitope... more OBJECTIVE: To explore whether the antibody subtypes against the extracellular 1-2 (EC1-2) epitopes of pemphigus vulgaris antigen (PVA) are related to the pathogenesis of PVA.METHODS: EC1-2 fusion protein, emulsified with complete Freund's adjuvant (CFA) or aluminum hydroxide hydrate [Al ( OH)3], was used to immunize C57BL/6 mouse. After immunization, the cytokine types, specific antibody titers, and antibody subtypes were detected. Also, a neonatal mice model was used to evaluate the pathogenesis of different antibodies.RESULTS: Th1 type cytokine interferon gamma (IFNgamma) was elevated in CFA group, while Th 2 type cytokine interleukin-4 (IL-4) was increased in Al (OH)3 group. The antibody subtypes were different in both groups. After the two groups were transferred with antisera separately, the neonatal mice developed erosion on the skin from Al(OH)3 group, with acantholysis histopathologically and bright immuno-fluorescence deposition, which was not seen in CFA group.CONCLUSION: Different antibody subtypes may contribute to the pathogenesis of disease.
Zhongguo Gu Shang China Journal of Orthopaedics and Traumatology, Dec 1, 2012
To evaluate efficacy of closed reduction and percutaneous hollow screw fixation with prototypical... more To evaluate efficacy of closed reduction and percutaneous hollow screw fixation with prototypical retractor in treating calcaneal fracture. From January 2009 to June 2011, 39 patients (43 feet) with calcaneal fracture were treated by closed reduction and percutaneous hollow screw fixation with prototypical retractor. There were 33 males and 6 females, aged from 19 to 61 years with an average of 36.7 years. According to type of Sanders, type II were in 19 feet, type III were in 24 feet. Preoperative and postoperative X-ray were estimated, the data of height, width, Bölher angle, Gissane angle of calcaneous were collected, and ankle function were estimated according to AOFAS system. All patients were followed up from 6 to 36 months with an average of (15.4 +/- 3.1) months. All wounds were healed well, no skin edge necrosis and infections occurred. Before operation, the height of calcaneous was average of (32.45 +/- 3.51) mm, width was (41.60 +/- 2.42) mm, Bölher angle was (8.64 +/- 13.2) degrees and Gissan angle was (136.35 +/- 15.23) degrees; while after operation, the height of calcaneous was average of (43.62 +/- 1.02) mm, width was (38.02 +/- 1.28) mm, Bölher angle was (26.87 +/- 5.32) degrees and Gissan angle was (120.78 +/- 5.34) degrees, and had significanty differences between preoperative and postoperative treatment (P<0.05). AOFAS score was improved from preoperative (35.64 +/- 11.23) to postoperative (76.18 +/- 9.87), and 29 cases got excellent results, 11 good and 3 fair. COMCLUSION: Closed reduction and percutaneous hollow screw fixation auxiliary by the retractor for the treatment is a good way, which has advantages of simple operation, satisfactory reduction fixation, reliable fixation, minimally invasive, less complications and rapid recovery.
Bulletin of the American Physical Society, Mar 6, 2015
The birth and the growth of Boson peak -insights from the normal modes analysis of granular exper... more The birth and the growth of Boson peak -insights from the normal modes analysis of granular experiments? JIE ZHANG, LING ZHANG, JIE ZHENG, Shanghai Jiaotong University -The origin of the Boson peak in amorphous materials has been a long-lasting puzzle for more than decades for researchers in the field. In order to understand the physics of the boson peak, we have experimentally measured the density of states (DOS) from the hexagonal lattice to the disordered structures in 2D packing of granular materials, which are made of photo-elastic disks allowing a precise measurement of contact forces between disks to determine the dynamical matrix of the system. These disks are wrapped with Teflon tapes to mimic frictionless particles so that the rotational degree of freedom can be ignored to a good approximation. By varying the pressure of the disordered crystal, we find the strong evidence that the first Van Hove singularity gradually evolves into the Boson peak. In geometrically disordered packing, the position of the Boson peak is influenced by the degree of the geometric disorder. Starting from a geometrically ordered crystal packing, a slight increase of the geometrical disorder will cause the Boson peak to have a sudden discontinues shift to a lower frequency. We find that such a shift might be related to the change of the topology of the strong and weak force networks in the system, which is currently still under investigation.
2013 Oceans San Diego, Sep 1, 2013
Advanced Materials Research, Dec 13, 2013
AgSnO2 contact materials have begun to replace toxic AgCdO materials in switching devices such as... more AgSnO2 contact materials have begun to replace toxic AgCdO materials in switching devices such as contactors and circuit breakers since 1970s [1,, which is partly due to the fact that AgSnO2 materials have shown higher service lives, better welding resistance and less arc erosion in contactors of larger size [. Furthermore, the growing awareness of environmental problems makes it desirable to replace AgCdO contact materials [. However, these materials suffer from an inadequate welding behavior compared with AgCdO [. It was observed that AgSnO2 made by traditional metallurgical process exhibited high overtemperatures attributed to high contact resistance caused by surface layers of accumulated SnO2, which was due to the poor wettability of SnO2 grains in the silver melt in combination with their high thermal stability [6,7]. Another important point was the large size and high hardness of SnO2 particle prepared by traditional process, which reduced the workability of AgSnO2 contact materials [8]. In this work, the authors prepared Ti4+-doped SnO2 using sol-gel technique and investigated the microstructure, size, distribution and crystallization of Ti4+-doped tin oxide grains as well as the doping effects on the electrical conductivity of these materials.
Solar Energy Materials and Solar Cells, 2012
The phase change behaviors of polyethylene glycol (PEG)/porous carbon composites are extensively ... more The phase change behaviors of polyethylene glycol (PEG)/porous carbon composites are extensively studied. Three types of shape-stabilized PCMs were prepared using expanded graphite (EG), active carbon (AC) and ordered mesoporous carbon (CMK-5) as supporting materials with various pore structures. They have distinct influences on the phase change behaviors of PEG/porous carbon composites. The highest stabilized PEG content is 70 wt% for AC and 90% for both EG and CMK-5. The phase change enthalpy and the PEG crystallinity increases in the order AC o CMK-5 o EG at the same PEG content in the composite. The study suggests that pores with micrometer size exhibit the same level of shape stabilization ability as the pores with nanometer size, while being able to minimize the enthalpy loss due to the interactions between the pores and the PEG chains. This study will provide insights into the design of composite PCMs through tailoring the pore structures.
Engineering, 2013
As there exists sorts of distributed generators in microgrid, an integrated control strategy cont... more As there exists sorts of distributed generators in microgrid, an integrated control strategy containing different control methods against corresponding generators should be applied. The strategy in this paper involves PQ control and droop control methods. The former aims at letting generators like PV output maximum power. The latter stems from inverter parallel technique and applies to controlling generators which can keep the network voltage steady to make the parallel system reach the minimum circulation point. Due to the unworthiness of droop control applied in low-voltage microgrid of which the impedance ratio is rather high, the paper adopts the droop control introducing virtual generator and virtual impedance. Based on theoretical analysis, simulation in Matlab is also implemented to verify the feasibility of the strategy.
The American journal of tropical medicine and hygiene
Data generated during the course of research activities carried out by the International Centers ... more Data generated during the course of research activities carried out by the International Centers of Excellence for Malaria Research (ICEMR) is heterogeneous, large, and multi-scaled. The complexity of federated and global data operations and the diverse uses planned for the data pose tremendous challenges and opportunities for collaborative research. In this article, we present the foundational principles for data management across the ICEMR Program, the logistics associated with multiple aspects of the data life cycle, and describe a pilot centralized web information system created in PlasmoDB to query a subset of this data. The paradigm proposed as a solution for the data operations in the ICEMR Program is widely applicable to large, multifaceted research projects, and could be reproduced in other contexts that require sophisticated data management.
Bioconjugate chemistry, Jan 14, 2015
In the native physiological environment, inorganic nanoparticles (NPs) often induce nonspecific p... more In the native physiological environment, inorganic nanoparticles (NPs) often induce nonspecific protein adsorption, which could significantly alter the function of the proteins they labeled. As a result, small fluorescent dyes are still widely used in the imaging of proteins in animals due to their minimal interference with protein function. Here, we used monomeric insulin as a model and compared its bioactivity before and after labeling with renal-clearable near-infrared-emitting gold NPs. These NPs were chosen because they have high resistance to serum protein adsorption and low nonspecific accumulation. We have found that a 1:1 insulin-NP ratio can be achieved, where the insulin-NPs show minimal serum protein binding with fully retained bioactivity comparable to that of unlabeled insulin. These results show a proof of concept that renal-clearable NPs can behave like small molecules in protein labeling without changing the individual protein's function, laying down a foundatio...
Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences
To evaluate the influence of pH values of fixatives and antigen retrieval buffers on immunostains... more To evaluate the influence of pH values of fixatives and antigen retrieval buffers on immunostains. Samples of normal skin, gastric mucosa, uteri leiomyoma and breast carcinoma were fixed in buffered formalin with different pH values, namely 3.0, 5.0, 7.0, 8.0 and 10.0 to detect EMA, SMA, ER and CK (AE1/AE3) expression. Evinision two-step method was employed. Antigen retrieval was achieved by microwave using sodium citrate solution with pH 6.0, 7.0, 8.0 or pH 8.0 EDTA solution respectively. The number and intensity of positive cells varied due to different conditions. The change of positive cell's number is important for CK (AE1/AE3) expression, and the change of positive intensity is important for SMA and EMA expression. (1) The best results could be obtained by the use of 10% neutral or alkalescent buffered formalin. (2) Antigen retrieval effect is better in the solution of pH 7.0, 8.0 than in that of pH 6.0. EDTA(pH 8.0) appears better for skin. The best effect of Estrogen Receptor needs pH 8.0 sodium citrate solution. (3) The background could be easily induced by the fixative over pH 8.0. Antigen retrieval solution over pH 8.0 could result in detachment.
Zhonghua bing li xue za zhi Chinese journal of pathology
To study effects of alternative transcripts of ING1 transfection on human cancer cell lines. p47/... more To study effects of alternative transcripts of ING1 transfection on human cancer cell lines. p47/ING1A and p33/ING1B expression vehicles were constructed and introduced into a human breast cancer cell line MCF-7 and a human lung cancer cell line PAa, both expressing wild-type p53 protein. Growth characteristics of the transfectants and potentially related genes were analyzed. The levels of p47/ING1A and p33/ING1B protein elevated respectively in tumor cells of MCF-7 and PAa after transfected with p47/ING1A and p33/ING1B, and the latter was much higher than that of the former. Ectopic overexpression of p33/ING1B effectively blocked tumor cell growth and arrested cells in the G(0) approximately G(1) phase of the cell cycle (P < 0.01), while p47/ING1A gave no effect on cell growth or cell cycle. Tumor cells overexpressing p33/ING1B contained more p21(WAF1) protein than that of the control cells, with undisturbed p53 protein level. Expression of two different transcripts of ING1 may have different effects on tumor cell growth. p33/ING1B may cooperate with p53 in stimulating expression of p21(WAF1) gene, thus to arrest cell cycle and to inhibit tumor cell growth. p33/ING1B may be considered to be a candidate as a partner of p53 in gene therapy.
Zhonghua bing li xue za zhi Chinese journal of pathology
To study the mechanism of antisense epidermal growth factor receptor cDNA in growth suppression o... more To study the mechanism of antisense epidermal growth factor receptor cDNA in growth suppression of glioblastomas cells. Glioblastoma U87MG cells, which over-express epidermal growth factor receptor (EGFR), were transfected with antisense-EGFR constructs. Several clones with stable expression of lower or undetectable levels of EGFR protein were obtained. The effect of antisense-EGFR on cell differentiation was studied using morphological evaluation and western blotting analysis of glial fibrillary acidic protein (GFAP) expression. The effect of antisense-EGFR on cell cycle was studied by flow cytometry and immunohistochemical analysis of p53, Rb, p16 and CDK4 expressions. The effect of antisense-EGFR on telomerase activity was studied by telomeric repeat amplification protocol (TRAP) assay. U87MG cells that were transfected with antisense-EGFR constructs had smaller cell bodies and longer processes, and expressed higher level of GFAP compared with that of the control cells. Flow cytometric analysis showed that the proportion of cells in G(0)/G(1) phases of the cell cycle in the antisense EGFR cDNA transfected clones increased significantly when compared with control cells, whereas the proportion of cells in S phase decreased markedly. In addition, immunohistochemical analysis showed that the expression of wild-type p53 was significantly increased in the antisense-EGFR cDNA transfected clones, whereas the expressions of Rb, p16 and CDK4 were not altered. TRAP assay revealed that telomerase activity in the antisense-EGFR clones was significantly decreased. Antisense-EGFR transfection inhibits U87MG cell growth by inducing cell differentiation and p53 expression, G(1) cell cycle arrest and inhibition of telomerase activity.
To look for a rapid low-cost technique for the detection of HBV variants.
2007 International Conference on Wireless Communications, Networking and Mobile Computing, 2007
... Proc., vol.3, pp.11251130, September 2002. [6] Nahdia Tabassum, AKM AhsanulHaque and Yoshiyo... more ... Proc., vol.3, pp.11251130, September 2002. [6] Nahdia Tabassum, AKM AhsanulHaque and Yoshiyori Urano, GSEN: An efficient energy consumption routing scheme for wireless sensor network, ICNICONSMCL'06, pp. 117 122. ...
Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences
To study the effects of antisense epidermal growth factor receptor (EGFR) cDNA transfection on te... more To study the effects of antisense epidermal growth factor receptor (EGFR) cDNA transfection on telomerase activity and telomere length of glioblastomas U87MG cells and the mechanism. Glioblastoma U87MG cells, which over-expressed EGFR, were transfected with antisense-EGFR cDNA constructs. Several clones stably expressing lower or undetectable levels of EGFR protein were obtained. The effect of antisense-EGFR cDNA on telomerase activity was assessed by Telomeric Repeat Amplification Protocol (TRAP) assay. The effect of antisense-EGFR cDNA on telomere length was determined by Southern blot hybridization. The mRNA expressions of hTERT, hTEP1 and c-myc were analyzed by semi-quantitative PCR. U87MG cells that were transfected with antisense-EGFR cDNA expressed lower level of EGFR and were less responsive to the growth stimulation of EGF compared with the control cells. Telomerase activity was significantly decreased in the antisense-EGFR clones. Telomere length was shortened. The mRNA expression of hTERT was slightly decreased in the antisense-EGFR clones, whereas the expressions of hTEP1 and c-myc were not altered. Antisense-EGFR cDNA transfection can sufficiently inhibit EGFR signal transduction pathway, decrease telomerase activity and shorten telomere length, which may be a new mechanism of antisense-EGFR approach in tumor suppression. The down-regulation of hTERT mRNA may contribute to the decreased telomerase activity in the antisense-EGFR clones.
Zhonghua Bing Li Xue Za Zhi Chinese Journal of Pathology, Feb 1, 2006
To study the clinicopathological features and differential diagnoses of mixed epithelial and stro... more To study the clinicopathological features and differential diagnoses of mixed epithelial and stromal tumor of the kidney. Clinical and pathological characteristics of 4 cases of mixed epithelial and stromal tumor of the kidney were studied. Three patients were female and one was male. All patients presented with flank pain and hematuria. Radiologic studies revealed cystic and solid masses involving the kidney. Grossly the tumors had a solid and cyst appearance. Microscopically, the tumors were composed of a mixture of stromal and epithelial elements. The epithelial elements were variable in cell types including cuboidal, hobnail and columnar cells. One case showed Müllerian and intestinal epithelial differentiations. Stromal elements essentially consisted of spindle cells, with thick-walled blood vessels and bands of smooth muscle cells as distinctive features of the tumor. Immunohistochemical staining revealed that the epithelial components were positive for AE1/AE3, whereas the stromal components were positive for ER, PR, and SMA. All patients underwent nephrectomy and were well without evidence of recurrence. Mixed epithelial and stromal tumor of the kidney is a benign neoplasm with distinct histopathological features. It should be distinguished from many other renal neoplasms. Surgical intervention is a preferred therapy.
2008 World Automation Congress, 2008
ABSTRACT
Progress in Biomedical Optics and Imaging, Feb 10, 2011
Zhongguo Yi Xue Ke Xue Yuan Xue Bao Acta Academiae Medicinae Sinicae, Apr 1, 2007
OBJECTIVE: To explore whether the antibody subtypes against the extracellular 1-2 (EC1-2) epitope... more OBJECTIVE: To explore whether the antibody subtypes against the extracellular 1-2 (EC1-2) epitopes of pemphigus vulgaris antigen (PVA) are related to the pathogenesis of PVA.METHODS: EC1-2 fusion protein, emulsified with complete Freund's adjuvant (CFA) or aluminum hydroxide hydrate [Al ( OH)3], was used to immunize C57BL/6 mouse. After immunization, the cytokine types, specific antibody titers, and antibody subtypes were detected. Also, a neonatal mice model was used to evaluate the pathogenesis of different antibodies.RESULTS: Th1 type cytokine interferon gamma (IFNgamma) was elevated in CFA group, while Th 2 type cytokine interleukin-4 (IL-4) was increased in Al (OH)3 group. The antibody subtypes were different in both groups. After the two groups were transferred with antisera separately, the neonatal mice developed erosion on the skin from Al(OH)3 group, with acantholysis histopathologically and bright immuno-fluorescence deposition, which was not seen in CFA group.CONCLUSION: Different antibody subtypes may contribute to the pathogenesis of disease.
Zhongguo Gu Shang China Journal of Orthopaedics and Traumatology, Dec 1, 2012
To evaluate efficacy of closed reduction and percutaneous hollow screw fixation with prototypical... more To evaluate efficacy of closed reduction and percutaneous hollow screw fixation with prototypical retractor in treating calcaneal fracture. From January 2009 to June 2011, 39 patients (43 feet) with calcaneal fracture were treated by closed reduction and percutaneous hollow screw fixation with prototypical retractor. There were 33 males and 6 females, aged from 19 to 61 years with an average of 36.7 years. According to type of Sanders, type II were in 19 feet, type III were in 24 feet. Preoperative and postoperative X-ray were estimated, the data of height, width, Bölher angle, Gissane angle of calcaneous were collected, and ankle function were estimated according to AOFAS system. All patients were followed up from 6 to 36 months with an average of (15.4 +/- 3.1) months. All wounds were healed well, no skin edge necrosis and infections occurred. Before operation, the height of calcaneous was average of (32.45 +/- 3.51) mm, width was (41.60 +/- 2.42) mm, Bölher angle was (8.64 +/- 13.2) degrees and Gissan angle was (136.35 +/- 15.23) degrees; while after operation, the height of calcaneous was average of (43.62 +/- 1.02) mm, width was (38.02 +/- 1.28) mm, Bölher angle was (26.87 +/- 5.32) degrees and Gissan angle was (120.78 +/- 5.34) degrees, and had significanty differences between preoperative and postoperative treatment (P<0.05). AOFAS score was improved from preoperative (35.64 +/- 11.23) to postoperative (76.18 +/- 9.87), and 29 cases got excellent results, 11 good and 3 fair. COMCLUSION: Closed reduction and percutaneous hollow screw fixation auxiliary by the retractor for the treatment is a good way, which has advantages of simple operation, satisfactory reduction fixation, reliable fixation, minimally invasive, less complications and rapid recovery.
Bulletin of the American Physical Society, Mar 6, 2015
The birth and the growth of Boson peak -insights from the normal modes analysis of granular exper... more The birth and the growth of Boson peak -insights from the normal modes analysis of granular experiments? JIE ZHANG, LING ZHANG, JIE ZHENG, Shanghai Jiaotong University -The origin of the Boson peak in amorphous materials has been a long-lasting puzzle for more than decades for researchers in the field. In order to understand the physics of the boson peak, we have experimentally measured the density of states (DOS) from the hexagonal lattice to the disordered structures in 2D packing of granular materials, which are made of photo-elastic disks allowing a precise measurement of contact forces between disks to determine the dynamical matrix of the system. These disks are wrapped with Teflon tapes to mimic frictionless particles so that the rotational degree of freedom can be ignored to a good approximation. By varying the pressure of the disordered crystal, we find the strong evidence that the first Van Hove singularity gradually evolves into the Boson peak. In geometrically disordered packing, the position of the Boson peak is influenced by the degree of the geometric disorder. Starting from a geometrically ordered crystal packing, a slight increase of the geometrical disorder will cause the Boson peak to have a sudden discontinues shift to a lower frequency. We find that such a shift might be related to the change of the topology of the strong and weak force networks in the system, which is currently still under investigation.
2013 Oceans San Diego, Sep 1, 2013
Advanced Materials Research, Dec 13, 2013
AgSnO2 contact materials have begun to replace toxic AgCdO materials in switching devices such as... more AgSnO2 contact materials have begun to replace toxic AgCdO materials in switching devices such as contactors and circuit breakers since 1970s [1,, which is partly due to the fact that AgSnO2 materials have shown higher service lives, better welding resistance and less arc erosion in contactors of larger size [. Furthermore, the growing awareness of environmental problems makes it desirable to replace AgCdO contact materials [. However, these materials suffer from an inadequate welding behavior compared with AgCdO [. It was observed that AgSnO2 made by traditional metallurgical process exhibited high overtemperatures attributed to high contact resistance caused by surface layers of accumulated SnO2, which was due to the poor wettability of SnO2 grains in the silver melt in combination with their high thermal stability [6,7]. Another important point was the large size and high hardness of SnO2 particle prepared by traditional process, which reduced the workability of AgSnO2 contact materials [8]. In this work, the authors prepared Ti4+-doped SnO2 using sol-gel technique and investigated the microstructure, size, distribution and crystallization of Ti4+-doped tin oxide grains as well as the doping effects on the electrical conductivity of these materials.
Solar Energy Materials and Solar Cells, 2012
The phase change behaviors of polyethylene glycol (PEG)/porous carbon composites are extensively ... more The phase change behaviors of polyethylene glycol (PEG)/porous carbon composites are extensively studied. Three types of shape-stabilized PCMs were prepared using expanded graphite (EG), active carbon (AC) and ordered mesoporous carbon (CMK-5) as supporting materials with various pore structures. They have distinct influences on the phase change behaviors of PEG/porous carbon composites. The highest stabilized PEG content is 70 wt% for AC and 90% for both EG and CMK-5. The phase change enthalpy and the PEG crystallinity increases in the order AC o CMK-5 o EG at the same PEG content in the composite. The study suggests that pores with micrometer size exhibit the same level of shape stabilization ability as the pores with nanometer size, while being able to minimize the enthalpy loss due to the interactions between the pores and the PEG chains. This study will provide insights into the design of composite PCMs through tailoring the pore structures.
Engineering, 2013
As there exists sorts of distributed generators in microgrid, an integrated control strategy cont... more As there exists sorts of distributed generators in microgrid, an integrated control strategy containing different control methods against corresponding generators should be applied. The strategy in this paper involves PQ control and droop control methods. The former aims at letting generators like PV output maximum power. The latter stems from inverter parallel technique and applies to controlling generators which can keep the network voltage steady to make the parallel system reach the minimum circulation point. Due to the unworthiness of droop control applied in low-voltage microgrid of which the impedance ratio is rather high, the paper adopts the droop control introducing virtual generator and virtual impedance. Based on theoretical analysis, simulation in Matlab is also implemented to verify the feasibility of the strategy.
The American journal of tropical medicine and hygiene
Data generated during the course of research activities carried out by the International Centers ... more Data generated during the course of research activities carried out by the International Centers of Excellence for Malaria Research (ICEMR) is heterogeneous, large, and multi-scaled. The complexity of federated and global data operations and the diverse uses planned for the data pose tremendous challenges and opportunities for collaborative research. In this article, we present the foundational principles for data management across the ICEMR Program, the logistics associated with multiple aspects of the data life cycle, and describe a pilot centralized web information system created in PlasmoDB to query a subset of this data. The paradigm proposed as a solution for the data operations in the ICEMR Program is widely applicable to large, multifaceted research projects, and could be reproduced in other contexts that require sophisticated data management.
Bioconjugate chemistry, Jan 14, 2015
In the native physiological environment, inorganic nanoparticles (NPs) often induce nonspecific p... more In the native physiological environment, inorganic nanoparticles (NPs) often induce nonspecific protein adsorption, which could significantly alter the function of the proteins they labeled. As a result, small fluorescent dyes are still widely used in the imaging of proteins in animals due to their minimal interference with protein function. Here, we used monomeric insulin as a model and compared its bioactivity before and after labeling with renal-clearable near-infrared-emitting gold NPs. These NPs were chosen because they have high resistance to serum protein adsorption and low nonspecific accumulation. We have found that a 1:1 insulin-NP ratio can be achieved, where the insulin-NPs show minimal serum protein binding with fully retained bioactivity comparable to that of unlabeled insulin. These results show a proof of concept that renal-clearable NPs can behave like small molecules in protein labeling without changing the individual protein's function, laying down a foundatio...
Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences
To evaluate the influence of pH values of fixatives and antigen retrieval buffers on immunostains... more To evaluate the influence of pH values of fixatives and antigen retrieval buffers on immunostains. Samples of normal skin, gastric mucosa, uteri leiomyoma and breast carcinoma were fixed in buffered formalin with different pH values, namely 3.0, 5.0, 7.0, 8.0 and 10.0 to detect EMA, SMA, ER and CK (AE1/AE3) expression. Evinision two-step method was employed. Antigen retrieval was achieved by microwave using sodium citrate solution with pH 6.0, 7.0, 8.0 or pH 8.0 EDTA solution respectively. The number and intensity of positive cells varied due to different conditions. The change of positive cell's number is important for CK (AE1/AE3) expression, and the change of positive intensity is important for SMA and EMA expression. (1) The best results could be obtained by the use of 10% neutral or alkalescent buffered formalin. (2) Antigen retrieval effect is better in the solution of pH 7.0, 8.0 than in that of pH 6.0. EDTA(pH 8.0) appears better for skin. The best effect of Estrogen Receptor needs pH 8.0 sodium citrate solution. (3) The background could be easily induced by the fixative over pH 8.0. Antigen retrieval solution over pH 8.0 could result in detachment.
Zhonghua bing li xue za zhi Chinese journal of pathology
To study effects of alternative transcripts of ING1 transfection on human cancer cell lines. p47/... more To study effects of alternative transcripts of ING1 transfection on human cancer cell lines. p47/ING1A and p33/ING1B expression vehicles were constructed and introduced into a human breast cancer cell line MCF-7 and a human lung cancer cell line PAa, both expressing wild-type p53 protein. Growth characteristics of the transfectants and potentially related genes were analyzed. The levels of p47/ING1A and p33/ING1B protein elevated respectively in tumor cells of MCF-7 and PAa after transfected with p47/ING1A and p33/ING1B, and the latter was much higher than that of the former. Ectopic overexpression of p33/ING1B effectively blocked tumor cell growth and arrested cells in the G(0) approximately G(1) phase of the cell cycle (P < 0.01), while p47/ING1A gave no effect on cell growth or cell cycle. Tumor cells overexpressing p33/ING1B contained more p21(WAF1) protein than that of the control cells, with undisturbed p53 protein level. Expression of two different transcripts of ING1 may have different effects on tumor cell growth. p33/ING1B may cooperate with p53 in stimulating expression of p21(WAF1) gene, thus to arrest cell cycle and to inhibit tumor cell growth. p33/ING1B may be considered to be a candidate as a partner of p53 in gene therapy.
Zhonghua bing li xue za zhi Chinese journal of pathology
To study the mechanism of antisense epidermal growth factor receptor cDNA in growth suppression o... more To study the mechanism of antisense epidermal growth factor receptor cDNA in growth suppression of glioblastomas cells. Glioblastoma U87MG cells, which over-express epidermal growth factor receptor (EGFR), were transfected with antisense-EGFR constructs. Several clones with stable expression of lower or undetectable levels of EGFR protein were obtained. The effect of antisense-EGFR on cell differentiation was studied using morphological evaluation and western blotting analysis of glial fibrillary acidic protein (GFAP) expression. The effect of antisense-EGFR on cell cycle was studied by flow cytometry and immunohistochemical analysis of p53, Rb, p16 and CDK4 expressions. The effect of antisense-EGFR on telomerase activity was studied by telomeric repeat amplification protocol (TRAP) assay. U87MG cells that were transfected with antisense-EGFR constructs had smaller cell bodies and longer processes, and expressed higher level of GFAP compared with that of the control cells. Flow cytometric analysis showed that the proportion of cells in G(0)/G(1) phases of the cell cycle in the antisense EGFR cDNA transfected clones increased significantly when compared with control cells, whereas the proportion of cells in S phase decreased markedly. In addition, immunohistochemical analysis showed that the expression of wild-type p53 was significantly increased in the antisense-EGFR cDNA transfected clones, whereas the expressions of Rb, p16 and CDK4 were not altered. TRAP assay revealed that telomerase activity in the antisense-EGFR clones was significantly decreased. Antisense-EGFR transfection inhibits U87MG cell growth by inducing cell differentiation and p53 expression, G(1) cell cycle arrest and inhibition of telomerase activity.
To look for a rapid low-cost technique for the detection of HBV variants.
2007 International Conference on Wireless Communications, Networking and Mobile Computing, 2007
... Proc., vol.3, pp.11251130, September 2002. [6] Nahdia Tabassum, AKM AhsanulHaque and Yoshiyo... more ... Proc., vol.3, pp.11251130, September 2002. [6] Nahdia Tabassum, AKM AhsanulHaque and Yoshiyori Urano, GSEN: An efficient energy consumption routing scheme for wireless sensor network, ICNICONSMCL'06, pp. 117 122. ...
Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences
To study the effects of antisense epidermal growth factor receptor (EGFR) cDNA transfection on te... more To study the effects of antisense epidermal growth factor receptor (EGFR) cDNA transfection on telomerase activity and telomere length of glioblastomas U87MG cells and the mechanism. Glioblastoma U87MG cells, which over-expressed EGFR, were transfected with antisense-EGFR cDNA constructs. Several clones stably expressing lower or undetectable levels of EGFR protein were obtained. The effect of antisense-EGFR cDNA on telomerase activity was assessed by Telomeric Repeat Amplification Protocol (TRAP) assay. The effect of antisense-EGFR cDNA on telomere length was determined by Southern blot hybridization. The mRNA expressions of hTERT, hTEP1 and c-myc were analyzed by semi-quantitative PCR. U87MG cells that were transfected with antisense-EGFR cDNA expressed lower level of EGFR and were less responsive to the growth stimulation of EGF compared with the control cells. Telomerase activity was significantly decreased in the antisense-EGFR clones. Telomere length was shortened. The mRNA expression of hTERT was slightly decreased in the antisense-EGFR clones, whereas the expressions of hTEP1 and c-myc were not altered. Antisense-EGFR cDNA transfection can sufficiently inhibit EGFR signal transduction pathway, decrease telomerase activity and shorten telomere length, which may be a new mechanism of antisense-EGFR approach in tumor suppression. The down-regulation of hTERT mRNA may contribute to the decreased telomerase activity in the antisense-EGFR clones.